• Applied Biological Chemistry
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• v.46 no.4
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• pp.333-337
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• 2003

This study was conducted to estimate the biofunctional characteristics, namely nitrite scavenging effect of barley leaves (BL) and their mixture (AM) with other antioxidants. Aqueous or methanol extracts were obtained from BL and AM. Aqueous and methanol extracts from barley leaves had relatively higher nitrite scavenging effects and its activities and contents increased in a concentration-dependent manner. The activities and contents of methanol extracts obtained from BL and AM were higher than those of aqueous extracts. Especially, AM containing BL and other antioxidant mixture had the highest activities and contents increased in a concentration-dependent manner. BL or AM were added to macrophage cells of RAW 264.7. Survival rates of the cells treated with BL and AM were measured to be different. $IC_{50}$ value decreased in a concentration-dependent manner by the addition of aqueous or methanol extracts from BL and AM. Especially, methanol extracts of AM had the highest nitrite scavenging effects. Thus, BL and AM may have protective effect against carcinogen and immune ability against reactive molecules through NO (nitric oxide) signal pathway. From the above results, barley leaves appear to contain natural anticancer and immune-related agents and may have potentiality to be used as functional food ingredients.

• Journal of Aquaculture
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• v.13 no.4
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• pp.285-293
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• 2000

Mortality suffered by the sea squirt in indoor experiment was higher at the stocking density of 12 kg than that at 3 and 6 kg. Daily mortality was also higher at 8 t:: thermal fluctuation than that at 3 and 5$^{\circ}C$. The thermal fluctuation observed at the depth of 5 m during June was higher, i.e. 7.4$^{\circ}C$ but it was just 2-4$^{\circ}C$ during mid-May. It did not fluctuate at all during the period from January to February. Mortality was higher at the stocking density of 12 kg during June than at 10 and 15 m depth.

• Korean Journal of Medicinal Crop Science
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• v.9 no.1
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• pp.8-14
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• 2001

The purpose of this research was to investigate the effects of extracts from cultured hairy roots of R. undulatum on human kidney epithelial cells. The cytotoxicity was measured by colorimetric assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), neutral red (NR) and sulforhodamine protein B (SRB) with human kidney epithelial cell lines A498. MTT, NR and SRB quantities decreased propotionally in cultured A498 cells treated with the water or chloroform extracts of cultured hairy roots at increasing concentrations. These results suggest that extracts of cultured hairy roots are cytotoxic on human epithelial cells. The cytotoxicity of chloroform fraction was stronger than that of water fraction. The values of $MTT_{50},\;NR_{50}\;SRB_{50}$ of the extracts of chloroform fraction and those of water fraction were measured to be $289.3{\mu}g/ml,\;302.7{\mu}g/ml,\;433.8{\mu}g/ml\;and\;475.8{\mu}g/ml,\;428.3{\mu}g/ml,\;549.5{\mu}g/ml$, in A498 cell line.

• 한국지구과학회:학술대회논문집
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• 2005.09a
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• pp.398-399
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• 2005

• Korean Journal of Materials Research
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• v.7 no.7
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• pp.559-563
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• 1997

Ta을 2MeV의 에너지로 가속시켜 1x$10^{17}$atoms/$\textrm{cm}^2$의 농도로 TiC(001)면에 이온 주입시킨 후 비행시간형 직충돌이온산란 분광법(time-of-flight impact-collision ion scattering spectroscopy; TOF-ICISS)을 사용하여 TiC(001)면의 Ta표면 편석을 연구하였다. TOF-ICISS는 표면 수층 깊이까지 원자구조를 측정할 수 있는 수법으로, 이온주입된 시편을 1$600^{\circ}C$에서 300sec동안 진공 가열하여 Ta 원자를 편석시킨 후 스펙트럼의 입사각도 의존성을 구함으로써 Ta원자의 편석 위치 및 농도구배를 조사하였다. [110]및 [100]방위에서 Ta과 Ti의 focusing peak가 서로 같은 입사각도에서 나타나며 편석된 Ta원자는 TiC의 Ti-site에 위치한다. Ta원자는 표면 최외층에만 편석되는 것이 아니라 수층에 걸쳐 Ti-site에 자리하고 있으며, Ta 원자의 농도는 표면 최외층에서 내부 층으로 깊어질수록 작아진다. 이온주입시 생성된 표면층의 탄소 격자 결함은 시편 가열시 벌크에 자리하는 탄소가 확산되어 없어진다.다.

• KSBB Journal
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• v.18 no.1
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• pp.45-50
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• 2003

In this study, the optimum conditions for mycelium culture of the mushroom Tricholoma matsutake and the inhibitory effect of the mycelium extracts no tyrosinase activity have been examined. When the extracts of the Tricholoma matsutake mycelia were tested for inhibitory activity on tyrosinase, it was found that the components extracted with ethyl acetate and water showed the highest inhibitory activity. The effect of antioxidants on the growth of mycelium and tyrosinase-inhibiting activity was also investigated. The results showed that tocopherol inhibited the growth in a concentration-dependent manner. In terms of tyrosinase-inhibiting activity, however, tocopherol was found to enhance the inhibitory activity.

• Proceedings of the Korea Water Resources Association Conference
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• 2019.05a
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• pp.282-287
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• 2019

본 연구의 목적은 음파를 이용하는 유속계로부터 관측된 후방산란강도와 실측된 부유사농도의 상관관계를 해석하여, 두 인자 사이의 관련 매개변수를 정량적으로 산정하고 관계식을 도출하는데 있다. 이를 위해 층별 유속계인 Aquadopp Profiler를 사용하여 사질 퇴적물 및 고령토 부유퇴적물 관측센서 검보정 챔버 실험을 수행하였다. 두 인자의 상관관계는 선형회귀분석을 통해 해석하였으며 그 결과로 도출된 접합곡선의 기울기는 각각 안목 A1 이 2.065, 안목 A2가 1.576, 고령토시료일 때 2.588의 값을 갖는데 이러한 차이는 시료 특성으로 설명될 수 있다. 서로 다른 입경을 갖는 시료는 후방산란 민감도에 차이가 있으며 이러한 영향으로 두 시료에서 산정된 계수 값에 차이가 발생한다. 본 연구를 통하여 도출된 측정 결과들은 후방산란강도에 미치는 수층 부유입자들의 영향이 매우 큼을 잘 보여준다. 일반적으로 수층 부유입자는 저면 퇴적물이 부유된 것으로 간주될 수 있으며, 또한 저면 퇴적물은 각 해역별로 site-specific 하다고 할 수 있다.

• Korean Journal of Environmental Biology
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• v.26 no.4
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• pp.392-401
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• 2008

The vertical changes (upper layer: 0$\sim$0.5 m, middle layer: 4$\sim$5 m and lower layer: 8$\sim$10 m) of the limnological variables, zooplankton dynamics and community filtration rate on the phytoplankton were evaluated monthly intervals from November 2004 to June 2007 at the artificiallake (Okjeong Lake). During the study, the annual mean of water temperatures were 15.2$\pm$7.8$^{\circ}C$ (upper layer), 14.5$\pm$7.4$^{\circ}C$ (middle layer) and 13.0$\pm$6.2$^{\circ}C$ (lower layer), respectively (n=30). The vertical variations of most limnological parameters (DO, pH, conductivity and turbidity) were fairly similar, except for chl.-${\alpha}$ concentration. Annual mean of chl.-${\alpha}$ concentration was highest at the upper layer (21.4 ${\mu}g$ L$^{-1}$), while annual means of concentrations were low at the middle layer and lower layer (4.3 ${\mu}g$ L$^{-1}$ and 3.5 ${\mu}g$ L$^{-1}$, respectively). Annual mean of Secchi depth was 261 cm (n=30). A total 45 species of zooplankton were identified (28 rotifers, 12 cladocerans and 5 copepods) during the study. The mean abundance of rotifers was highest (124$\pm$232 ind. L$^{-1}$, n =90) and that of cladocerans and copepods were relatively low (22$\pm$56 ind. L$^{-1}$ and 13$\pm$30 ind. L$^{-1}$) in the whole layer. Total zooplankton abundance increased from late spring to summer, and then declined and remained low throughout the winter. The annual mean of total zooplankton filtration rate for phytoplankton at the lower layer was much higher than that of both layers (upper layer: 44.4$\pm$107.8, middle layer: 95.1$\pm$436.4 and lower layer: 158.2$\pm$436.4 mL L$^{-1}$ day$^{-1}$). Among the major zooplankton community, relative community filtration rate (RCFR, %) of copepods for phytoplankton (50.2$\sim$54.8%) was much higher than that of cladocerans (27.7$\sim$36.3%) and rotifers (8$\sim$17.6%). The seasonal variation of RCFRs of major zooplankton community was observed. The RCFRs of rotifers were high in winter (44.6%, n=9), while the RCFRs of cladocerans were high in summer (58.0%, n=7). In spring and fall, the RCFRs of copepods were high in the whole layer (spring: 67.4%, n=9; fall: 74.4%, n=5).

• The Korean Journal of Food And Nutrition
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• v.24 no.4
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• pp.528-534
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• 2011

This study was conducted to investigate the changes in saponin content and antioxidant activity of crude ginseng and extruded ginseng by using different solvent extraction methods. Each of the fractions was first extracted by 80% ethanol followed by ether treatment to remove the lipid components. Water soluble components were separated by ethylacetate and water saturated butanol. Four fraction, including 80% ethanol, ethylacetate, butanol and water were obtained from crude and extruded ginsengs to analyze saponin content and antioxidant activity. Saponin content and antioxidant capacity of each of the four fractions were measured by LC/MS analysis and ORAC(Oxygen Radical Absorbance Capacity) assay, respectively. It was found that a major portion of saponin was present in ethyl acetate and water saturated butanol fractions. When extracted by 80% ethanol, ginsenoside Rb1 and Rg1 were mostly found in crude ginseng, while ginsenoside Re and Rb1 were detected in extruded ginseng. Even though Rh1 and Rg3 were found in a very small quantity in crude ginseng, there was a significant quantity of both in extruded ginseng when extracted by 80% ethanol. Similar tendency was also observed in extruded ginseng fraction when extracted with ethyl acetate and butanol. In crude ginseng, the level of Rg1 was the highest among other ginsenosides upon extraction by ethyl acetate, while Rh1 and Rg3 were predominantly found by employing similar solvent extraction in the extruded ginseng. Also, Rg1, Re and Rb1 were also found in the extruded ginseng with small quantity. Rg1, Re and Rb1 were found in crude ginseng by butanol extraction, while Rb1 and Re were extracted from the extruded ginseng. Overall, there was no difference in the saponin content between crude ginseng and extruded ginseng when extracted by butanol and water, but twice as much of saponin was obtained by 80% ethanol extraction and 6 times more saponin were obtained in ethyl acetate fraction in the extruded ginseng. Antioxidant capacity of crude ginseng as determined by ORAC assay was higher in 80% ethanol(high in many different kinds of biological compounds) and water saturated butanol(high in polar saponin) fractions than the ethyl acetate and water fractions. No difference in antioxidant capacity was observed between crude and extruded ginseng. However, antioxidant capacity of ethyl acetate and water fractions in extruded ginseng was significantly higher than crude ginseng($P$ >0.05). All the fractions in both, crude and extruded ginseng possessed antioxidant capacity and even water fractions that contained almost no saponin had some antioxidant capacity. While determining correlation coefficient between fractions in extruded ginseng by Pearson correlation, it was observed that 80% ethanol fraction was in correlation with ethyl acetate($P$ >0.01) and ethanol($P$ >0.001) and in the case of ethylacetate, correlation was observed only with butanol fraction($P$ >0.05).

• Journal of Life Science
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• v.22 no.7
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• pp.889-896
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• 2012

Melanin synthesis is catalyzed by tyrosinase. To investigate the whitening effect of Hizikia fusiformis, fractions from ethanol extract of H. fusiformis were prepared by a systematic fractionation procedure with solvents such as methanol, hexane, butanol, and $H_2O$. The ethanol extract and its fractions were then subjected to evaluate the inhibitory effects on the tyrosinase activity and melanin synthesis in murine B16F10 melanoma cells. The ethanol extract and aqueous fraction exhibited a whitening effect with no cytotoxicity. The ethanol extract showed the highest whitening effect among the samples. The inhibitory effect of $100{\mu}g/ml$ of ethanol extract was higher than that of $10{\mu}g/ml$ of arbutin, but it was lower than that of $10{\mu}g/ml$ of kojic acid. Furthermore, the inhibitory effects of $100{\mu}g/ml$ of methanol, hexane, butanol, and aqueous fractions were similar to those of $10{\mu}g/ml$ of arbutin. The antioxidant activities were examined by comparing the results with that of ascorbic acid as a positive control. The ethanol extract and aqueous fraction showed relatively higher DPPH radical-scavenging activities compared with the other samples. Furthermore, $500{\mu}g/ml$ of ethanol extract and aqueous fraction diminished LPS-induced iNOS expression to 82 and 80%, respectively. These results suggest that ethanol extract and aqueous fraction of H. fusiformis could be used as cosmetic ingredients for whitening and skin protection effects.