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http://dx.doi.org/10.5352/JLS.2012.22.7.889

Whitening Effect of Hizikia fusiformis Ethanol Extract and Its Fractions  

Jeon, Myong-Je (Department of Bioscience and Biotechnology, Graduate School, Silla University)
Kim, Mi-Hyang (Department of Food and Nutrition, Silla University)
Jang, Hye-Ji (Department of Bioscience and Biotechnology, Graduate School, Silla University)
Lee, Seung-Woo (Department of Bioscience and Biotechnology, Graduate School, Silla University)
Kim, Jae-Hoon (Haeim Pharmetic Co., Marine-Bio Center)
Kim, Hyung-Suk (Haeim Pharmetic Co., Marine-Bio Center)
Lee, Sang-Hyeon (Department of Bioscience and Biotechnology, Graduate School, Silla University)
Publication Information
Journal of Life Science / v.22, no.7, 2012 , pp. 889-896 More about this Journal
Abstract
Melanin synthesis is catalyzed by tyrosinase. To investigate the whitening effect of Hizikia fusiformis, fractions from ethanol extract of H. fusiformis were prepared by a systematic fractionation procedure with solvents such as methanol, hexane, butanol, and $H_2O$. The ethanol extract and its fractions were then subjected to evaluate the inhibitory effects on the tyrosinase activity and melanin synthesis in murine B16F10 melanoma cells. The ethanol extract and aqueous fraction exhibited a whitening effect with no cytotoxicity. The ethanol extract showed the highest whitening effect among the samples. The inhibitory effect of $100{\mu}g/ml$ of ethanol extract was higher than that of $10{\mu}g/ml$ of arbutin, but it was lower than that of $10{\mu}g/ml$ of kojic acid. Furthermore, the inhibitory effects of $100{\mu}g/ml$ of methanol, hexane, butanol, and aqueous fractions were similar to those of $10{\mu}g/ml$ of arbutin. The antioxidant activities were examined by comparing the results with that of ascorbic acid as a positive control. The ethanol extract and aqueous fraction showed relatively higher DPPH radical-scavenging activities compared with the other samples. Furthermore, $500{\mu}g/ml$ of ethanol extract and aqueous fraction diminished LPS-induced iNOS expression to 82 and 80%, respectively. These results suggest that ethanol extract and aqueous fraction of H. fusiformis could be used as cosmetic ingredients for whitening and skin protection effects.
Keywords
Hizikia fusiformis; melanin; tyrosinase activity; whitening effect;
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