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Functional Characteristics from the Barley Leaves and its antioxidant mixture - Study on the Nitrite Scavenging Effect -  

Hong, I-Jin (Aminogen Co. Ltd., Cancer Research Institute, College of Medicine, Seoul National University)
Park, Hyeung-Geun (Department of Pharmacy, Seoul National University)
Jew, Sang-Sup (Department of Pharmacy, Seoul National University)
Kim, Kyung-Tack (Department of Ginseng Science Research, Korea Food Research Institute)
Lee, Sung-Hee (Aminogen Co. Ltd., Cancer Research Institute, College of Medicine, Seoul National University)
Publication Information
Applied Biological Chemistry / v.46, no.4, 2003 , pp. 333-337 More about this Journal
Abstract
This study was conducted to estimate the biofunctional characteristics, namely nitrite scavenging effect of barley leaves (BL) and their mixture (AM) with other antioxidants. Aqueous or methanol extracts were obtained from BL and AM. Aqueous and methanol extracts from barley leaves had relatively higher nitrite scavenging effects and its activities and contents increased in a concentration-dependent manner. The activities and contents of methanol extracts obtained from BL and AM were higher than those of aqueous extracts. Especially, AM containing BL and other antioxidant mixture had the highest activities and contents increased in a concentration-dependent manner. BL or AM were added to macrophage cells of RAW 264.7. Survival rates of the cells treated with BL and AM were measured to be different. $IC_{50}$ value decreased in a concentration-dependent manner by the addition of aqueous or methanol extracts from BL and AM. Especially, methanol extracts of AM had the highest nitrite scavenging effects. Thus, BL and AM may have protective effect against carcinogen and immune ability against reactive molecules through NO (nitric oxide) signal pathway. From the above results, barley leaves appear to contain natural anticancer and immune-related agents and may have potentiality to be used as functional food ingredients.
Keywords
barley leaves; nitrite scavenging effect; immune; nitric oxide;
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1 Yoshihide, H. and Takarazuka, H. (1974) Process for producing powders of green leaves of wheat and barley. U.S. Patent 3,787,591
2 Lee, Y.C., Son, J.Y, Kim, K.T. and Kim S.S. (1994) Antioxidant activity of solvent extract isolated from barley leaves. Korean J. Food Nutr. 1, 332-337
3 Kato, H., Lee, I. E., Chuyen, N. V., Kim, S. B. and Hayase, F. (1987) Inhibidon of nitrosamine formation by nondialyzable melanoidins. Agric. Bio. Chem. 51, 1333-1338   DOI
4 Chung, S. Y, Kim, N. K. and Yoon, S. (1999) Nitrite scavenging effect of methanol fraction obtained from green yellow vegetable juices. J. Korean Soc. Food Sci. Nutr. 28, 342-347
5 Hibbs, J. B., Taintor, R. R., Vavrin, Z. and Rachlin, C. (1988) Nitric oxide. A cytotoxic acdvated macrophage effector molecule. Biochem. Biophys. Res. Commun. 157, 87-94   DOI   ScienceOn
6 Kim, K. T., Soek, H. M. and Kim, S. S. (1994) Changes in physicochemical charactehstics of barley leaves during growth. Korean J. Food Sci. Technol. 26, 471-474
7 Furchgott, R. F., and Zawadzki, J. V. (1980) The obligatory role of endothelial cells in the relazation of arterial smooth muscle by acetylcholine. Nature 288, 373-376   DOI   ScienceOn
8 Mellion, B. T., Ignatto, L. J., Ohlstein, E. H., Pontecorvo, E. G., Hyman, A. L. and Kadowitz, P. J. (1981) Evidence for the inhibiroty role of guanosine 3',5'-monophosphate in ADP induced human platelet aggregadon in the presence of nitric oxide and related vasodilators. Blood 57, 946-955
9 Lee, Y. J. and Han, J. P (2000) Antioxidadve acdvities and nitrite scavenging abilities of extracts from Ulmus devidiana. J. Korean Soc. Food Sci. Nutr. 29, 893-899
10 Lee, J. M. and Ahn, M. S. (1997) A study on nitrite scavenging ability of tea extracts. Korean J. Diet. Cult. 12, 567-572
11 Kao, F. T. and Puck, T. T. (1971) Mutagenesis by carcinogenic nitroso compounds. J. Cell. Physiol. 78, 139-144   DOI   PUBMED
12 Gray, J. I. And Dugan, J. R. (1975) hihibition of N-nitrosamine formation in model food systems. J. Food Sci. 40, 981-984   DOI
13 Iyengar, R., Stuehr, D. J., and Marletta, M. A. (1987) Macrophage synthesis of nitrite, nitrate and N-nitrosamines: Precursors and role of the respiratory burst. Proc. Natl. Acad. Sci. USA 84, 6369-6376   DOI   ScienceOn
14 Kator, K. (1988) In Aging: Active oxygen-molecular mechanism of its productionscavenging and effect in organism. Kyourits publishing Co, Tokyo, Japan
15 Byers, T. and Perry, G. (1992) Dietary carotenes, vitamin C and vitamin E as protective antioxidants in human cancers. Annu. Rev. Nutr. 12, 135-159
16 Bret, D. S. and Snyder, S. H. (1992) Nitric oxide: a novel neuronal messenger. Neuron 8, 8-11
17 Bartsh, H., Ohshima, H. and Pignatell, B. (1988) Inhibition of endogenous nitrosation: Mechanism and implicadons in human cancer prevention. Mutat. Res. 202, 307-324   DOI
18 Leaf, C. D., Wishnok, J. S. and Tannenbaum, S. R. (1989) Mechanisms of endogenous nitrosadon. Cancer Surv. 8, 323-334
19 Toshihiko, O., Hirataka K., Yoshihide H., Hideaki H. and Takayuki S. (1992) A novel antioxidant isolated from young green barley leaves. J. Agric. Food Chem. 40, 1135-1138
20 Greenblatt, M., Mirvish, S. and So, B. T. (1971) Nitrosamine studies: Inducdon of lung adenomas by concurrent administration of sodium nitrite and secondary amines in Swiss mice. J. Nat. Cancer Inst. 46, 1029-1034