• Title/Summary/Keyword: 수정능력

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Effects of $\beta$-Mercaptoethanol on lipid Peroxidation and Fertilization Ability In Vitro by Xanthine-Xanthine Oxidase System in Pig (Xanthine-Xanthine Oxidase System,하에서 돼지 동결-융해정자의 Lipid Peroxidation과 체외수정능력에 대한 $\beta$-Mercaptoethanol의 영향)

  • 사수진;정희태;이장희;유일선;양부근;김정익;박춘근
    • Korean Journal of Animal Reproduction
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    • v.26 no.3
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    • pp.263-273
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    • 2002
  • This study was undertaken to evaluate the effects of $\beta$-mercaptoethanol ($\beta$-ME) on lipid peroxidation and fertilization ability in vitro by xanthine (X) - xanthine oxidase (XO) system in boar spermatozoa frozen-thawed. The boar spermatozoa were treated with X and/or XO, and the spermatozoa viability were measured by the eosin-nigrosin stain method. In control group, level of vitality in boar spermatozoa were higher than in medium with X, XO and X+XO groups. No significant differences, however, were observed under the all conditions. The percentage of spermatozoa that reached acrosome reaction were significantly (P<0.05) higher in sperm treated without that than with $\beta$-ME under the all conditions. On the other hand, when spermatozoa were inseminated in medium with X and/or XO, the penetration rates in all conditions were higher in medium with that than without $\beta$-ME. However, significant differences were not observed between medium with and without $\beta$-ME. The lipid peroxidation of sperm was evaluated on the basis of malondialdehyde (MDA) production. The MDA were higher in sperm treated without that than with $\beta$-ME under the above all conditions. However, significant differences were not observed between medium with and without $\beta$-ME. Sperm-SH group were higher detected in medium with that than without $\beta$-ME under the all conditions. The activity of sperm binding to Bona pellucida was also evaluated through binding to salt-stored porcine oocytes. In control group, sperm binding to zona pellucida were significantly (P<0.05) higher than in medium with X+XO groups. The sperm binding in all conditions were higher in medium with that than without $\beta$-ME. However, significant differences were not observed between medium with and without $\beta$-ME. These results suggest that addition of $\beta$-ME in X-XO system may play a positive role in improving of fertilization ability in vitro.

Changes of Glycosidase Activity and Fertilizing Ability in Vitro by Incubation of Frozen-Thawed Spermatozoa in the Pig (돼지 동결정액의 배양에 따른 체외수정능력과 Glycosidase Activity의 변화)

  • 황인선;정희태;양부근;김정익;박춘근
    • Korean Journal of Animal Reproduction
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    • v.27 no.2
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    • pp.153-161
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    • 2003
  • This study has evaluated effect of the spermatozoa incubation on the glycosidase activity and fertilizing ability in vitro in the pig. To identify sperm glycosidases specific for sugar residues found in the zona pellucida of pig oocytes, the spermatozoa were treated experimentally and assayed for activities of $\alpha$-L-fucosidase, $\alpha$-D-mannosidase, $\beta$-D-galactosidase and N-acetyl-$\beta$-D-glucosaminidase ($\beta$-GlcNAc'ase). The glycosidases activity were higher in spermatozoa incubated for 2h than without incubation. The $\beta$-GlcNAc'ase activity was at least two-fold higher than other glycosidase regardless of spermatozoa incubation. In the same glycosidases, the activity had a tendency to increase as time of spermatozoa incubation was prolonged, but there were no differences in spermatozoa incubated during the various periods (4~24h). The percentages of spermatozoa that reached acrosome reaction were affected by glycosidases in the medium (P<0.05, for mannosidase), and were higher in spermatozoa with that than without incubation. On the other hand, the spermatozoa motility were decreased with incubation periods, but no effects by different glycosidases on the change of sperm motility during the various periods of incubation. In other experiment, the binding and penetration of pig spermatozoa were tested with oocytes matured in vitro in the presence of various glycosidase. The penetration rates were decreased with incubation of spermatozoa when oocytes were inseminated in medium with different glycosidases. These rates were higher in spermatozoa non-incubated than with incubation for 2h (P<0.05 for GlcNAc'ase; P<0.01 for control group). The sperm-zona binding rate in control group were higherthan in medium with glycosidases. In addition, the highest binding rate were obtained in medium with GlcNAc'ase. In all glycosidases, the sperm-zona binding rate in spermatozoa without incubation were higher than incubation for 2h. The significant differences were obtained in spermatozoa treated with $\alpha$-D-mannosidase (P<0.05). These results suggest that $\beta$-GlcNAc'ase is present mainly in the plasma membrane of pig spermatozoa. It was also shown that the glycosidase activity were increased in all glycosidases in spite of low sperm-zona binding rate and penetration rates by spermatozoa incubation.

A Study on the Past that Work Scope of Medical Interpreter Professional Personnel -Focusing on the Range of Possible Questions for the Medical Translation Ability Test (의료통역전문인력 업무범위에 대한 소고 -의료통역능력검정시험 출제범위 중심으로)

  • Kim, Seung Chul;Kim, Tae-Hyung;Lee, Yeon-Kyung
    • The Journal of the Korea Contents Association
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    • v.20 no.4
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    • pp.571-581
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    • 2020
  • There are large, medium, and small items in the evaluation test for medical interpreter professionals personnel and the criteria and level are not clear, which may cause confusion for those preparing for the test. Therefore, we would like to suggest that the qualification requirements for the medical translation ability test and the criteria for the evaluation items are consistent with the medical system in Korea. The survey on the medical interpreter competency test conducted was collected from domestic and foreign data, compared with similar test and overseas test. We also examined the perception of the test by experts with experience in developing and interpreting the medical interpretation test. As a result, in the 'International Culture' evaluation category, 'Language-oriented culture' was changed to 'Medical-oriented culture' and 'Interpretation ethics' was changed to 'Medical interpretation ethics'. In the evaluation items of the hospital system, the 'Medical Dispute Mediation Act', which is 「ACT ON MEDIATION OF MEDICAL DISPUTES」 of the middle item was changed the 「ACT ON REMEDIES FOR INJURIES FROM MEDICAL MALPRACTICE AND MEDIATION OF MEDICAL DISPUTES」 and the Act also reduced the four items related to the 'Medical Tourism Law' to two and added the 「ACT ON SUPPORTING THE ADVANCEMENT OF MEDICAL OVERSEAS AND ATTRACTING FOREIGN PATIENTS」. If the Medical Interpretation Proficiency Test is prepared in accordance with the medical culture of Korea, it is expected that there will be a stable opportunity for professionals who pass the examination to act as experts.

The Effects of Functional Task Training on Activities of Daily Living in the Fall Experienced Elderly (기능적 과제훈련이 낙상경험노인의 일상생활수행능력에 미치는 영향: 단일대상연구)

  • Woo, Ji Hee;Park, Hae Yean;Kim, Jong Bae
    • Therapeutic Science for Rehabilitation
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    • v.5 no.1
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    • pp.43-54
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    • 2016
  • Objective : The purpose of this study was to investigate the effects of occupational therapeutic intervention including functional task training on activities daily living of falls experienced elderly. Methods : This study used a single subject experimental design with alternating treatment and reversal design. One participant who have had falls experience among the community-dwelling elderly completed. This study a total of 18 sessions were conducted. Fall Efficiency Scale(FES), Berg Balance Scale(BBS) and task modification scale were used to assess the activities of daily living during each session, and Canadian Occupational Performance Measure(COPM) and Assessment of Home-based Activities(AHA) were used to evaluate the activities of daily living at pre-and post-test. Results : The participant showed significant improvements in the FES, BBS scores. However, the improvement was greater when progressive resistance exercise was practised along with functional task training. COPM and AHA scores improved at post-test. Conclusions : These findings provide evidence for occupational therapeutic intervention for falls-experienced elderly.

The Effects of Clinical Convergence Self-directedness Practice Learning Program on Self-directedness and Competency in Fundamental Nursing Skills in Undergraduate Nursing Students (임상융합 자기주도적 실습교육 프로그램이 간호대학생의 자기주도성 및 기본간호술기 수행능력에 미치는 영향)

  • Park, Jihyun
    • Journal of the Korea Convergence Society
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    • v.7 no.4
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    • pp.51-58
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    • 2016
  • The purpose of this study was to examine the effects of clinical convergence self-directedness practice learning program on self-directedness and competency in fundamental nursing skills in undergraduate nursing students. The study design was a non-equivalent pre-post quasi-experimental design. The participants were 80 nursing students in D city. The experimental and control groups were randomly assigned with 40 participants in each group. Interventions for the experimental group were visiting clinical practice, video recording and students' self-debriefing of what they did over the 5 days. Self-directedness and competency in fundamental nursing skills were measured 2 times (pre-, and post-intervention) over the 5 days. Independent t-test, Mann-Whitney U test and chi-square test were used for data analyses. There was statistically significant difference for self-directedness(t=12.93, p=.001) and competency in fundamental nursing skills(t=8.34, p=.021). Results indicate that self-debriefing method using video recording is an effective learning way to improve Self-directedness and competency in fundamental nursing skills in nursing students.

The Effect of Occlusal Condition on Physical Fitness and Motor Capacity in Athletes According to Various Types of Mouthguards (마우스가드의 형태가 운동선수의 체력 및 운동능력에 미치는 영향)

  • Choi, Su-Jeong;Jung, Jae-Kwang;Lee, Kyu-Bok;Chae, Woen-Sik
    • Journal of Dental Rehabilitation and Applied Science
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    • v.29 no.1
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    • pp.1-9
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    • 2013
  • This study examined the effects of the occlusal stability and a partial coverage mouthguard on physical fitness and motor capacity to determine the importance of the occlusal stability as a possible action mechanism of mouthguards on physical performance. Twenty physical education students were included for measurements of their handgrip strength, back strength, whole body reaction time, flexibility, sidestep test, stork stand test and jumping test according to the following 5 different occlusal conditions: mouth closed position without a mouthguard, with a full coverage mouthguard, with a right partial coverage mouthguard, with a left partial coverage mouthguard and with anterior partial mouthguard. The results revealed no significant difference in any of the measured physical factors between the occlusal conditions with and without a full-coverage mouthguard. On the other hand, a significant difference was observed in whole body reaction between the occlusal conditions with and without the partial coverage mouthguards. Therefore, isokinetic muscle tests on both knee joints and the Wingate anaerobic power test should be performed under the following five occlusal conditions: with or without full-coverage maxillary custom-made mouthguard, with a unilateral right or left partial-coverage maxillary mouthguard and with an anterior partial-coverage maxillary mouthguard. These results suggest that the partial coverage mouthguard had a short-term beneficial effect on agility rather than full coverage mouthguard.

Effect of The Trunk Muscle Stabilization Training on Balance for Chronic Stroke Patients (체간 근육 안정화 운동이 만성 뇌졸중 환자의 균형에 미치는 영향)

  • Lee, Ji-Yeun;Park, Jung-Seo;Lee, Dae-Hee;Han, Seul-Ki;Roh, Hyo-Lyun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.13 no.3
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    • pp.1212-1219
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    • 2012
  • This study was to investigate the balancing and clinical abilities trunk muscle stabilization exercise and trunk muscle resistive exercise of stroke patients having surface changes in balance of body. The subjects of this study were 30 stroke patients(15 males and 15 females) that three times a week for 12 weeks at the same time point and with the same amount of exercise. They were separated into two groups and did reinforcing exercise on trunk muscle stabilization exercise and trunk muscle resistive exercise for three times a week for twelve weeks. It were measured balancing ability, they performed functional standing balance test, functional forward arm stretching test. performance-oriented mobility assessment, berg balance scale. The balancing exercises were modified from preceding studies and consisted of 6 difference exercises. The functional standing test with open and close eyes and performance-oriented mobility assessment, the trunk muscle stabilization exercise group and the trunk muscle resistive exercise group improved significantly. The forward arm stretching was test the trunk muscle stabilization exercise group more improved. This study found that the balancing exercise on trunk muscle stabilization exercise was more effective than trunk muscle resistive exercise for stroke patients. Thus, trunk muscle stabilization exercise has more positive effects on the improvement of balancing abilities of stroke patients.

Preliminary Home Economics Teachers' Image of Home Economics (예비 가정교사들의 가정교과에 대한 이미지의 유형)

  • Wang, Sek-Sun;Nam, Su-Jung
    • Journal of Korean Home Economics Education Association
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    • v.20 no.4
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    • pp.205-211
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    • 2008
  • This study attempted to explore what is the image that preliminary teachers seek in Home Economics. The purpose of this study was to find the images that make people thought based on the Q methodology. Q methodology is a way to understand a person's structure of subjectivity. This study applied Q methodology to clarifying preliminary teachers' subjective evaluation of the image of Home Economics. 33 Q questions were prepared through literature review, and were offered to 60 preliminary teachers. The subjects were divided into the five types seeking different image with Home Economics: adjustment for society, neglection by prejudice, education for basic element of life, focused on home life, personal mature.

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Seismic Performance of Special Shear Wall with Modified Details in Boundary Element Depending on Axial Load Ratio (축력비에 따른 수정된 단부 횡보강상세를 갖는 특수전단벽의 내진성능)

  • Chun, Young-Soo;Park, Ji-Young
    • Land and Housing Review
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    • v.7 no.1
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    • pp.31-41
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    • 2016
  • In this paper, we propose experimental results, which target the major variables that influence the structural performance of a wall, as well as the resulting seismic and hysteretic behavior. Results also provide the basis for the application of performance based design by identifying the nonlinear hysteretic behavior of the wall with boundary element details recently proposed in previous study by Chun et al(2011). From the experimental results, the crack and fracture patterns of a specimen, which adopt the proposed boundary element details, showed similar tendencies regardless of whether axial force or high performance steel bars is applied. Furthermore, results show that the maximum strength of the specimen can be predicted accurately based on the design equation proposed by the standard. In addition, with a higher axial force, there is a tendency that both the initial load and maximum strength increase as deformation capacity reduces, requiring consideration of the reduced deformation capacity due to a high axial force. For walls under such high axial forces, using high performance steel bars is a very effective manner of enhancing deformation capacity. Therefore, reinforcing the plastic hinge region with boundary elements using high performance steel bars is preferable.

Nonlinear Analysis of RC Members Using Truss Model (트러스 모델을 이용한 철근콘크리트 부재의 비선형해석)

  • Eom, Tae-Sung;Park, Hong-Gun
    • Journal of the Korea Concrete Institute
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    • v.18 no.2 s.92
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    • pp.177-188
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    • 2006
  • Conventional nonlinear finite element analysis requires complicated modeling and analytical technique. Furthermore, it is difficult to interpret the analytical results presented as the stress-strain relationship. In the present study, a design-oriented analytical method using the truss model was developed. A reinforced concrete member to be analyzed was idealized by longitudinal, transverse, and diagonal line elements. Basically, each element was modeled as a composite element of concrete and re-bars. Simplified cyclic models for the concrete and re-bar elements were developed. RC beams and walls with various reinforcement details were analyzed by the proposed method. The inelastic strength, energy dissipation capacity, deformability, and failure mode predicted by the proposed method were compared with those of existing experiments. The results showed that the proposed model accurately predicted the strength and energy dissipation capacities, and to predict deformability of the members, the compression-softening model used for the concrete strut element must be improved.