• Title/Summary/Keyword: 셀룰라아제

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Deinking process of Old Newsprint(ONP) by using Modified Cellulase with synthesized copolymer (기능성 고분자를 이용한 수식 셀룰라아제의 폐 신문용지 탈묵에 관한 연구)

  • Kim, Honghyun;Kwak, Tae-Heon;Park, Jinwon;Park, Kwinam
    • Clean Technology
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    • v.10 no.4
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    • pp.195-201
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    • 2004
  • Cellulase was modified with copolymer with polyethylene(PE)/polypropylene(PP) oxide and maleic anhydride(MA) by maleylation reaction, and modified cellulase was applied to the reprocessing of old newsprint (ONP). Cellulase of modified cellulase enhanced the detachment of ink particles by fibrillation of fiber. The copolymer, which acted as the surfactant formed bubbles and removed the ink particles in the floatation process. Modified cellulase showed the same deinking ability without excess dosage compared with the conventional method. And, it improved the physical properties including tensile strength, brightness, and whiteness compared with the conventional deinking process. The bond between the ink and fiber got stronger as the storage time increased, and it became very difficult to remove the ink particle. But, modified cellulase increased the deinking ability by 41% compared with the conventional process at the experiment of the ONP for 1 year storage time. It removed the yellowing and increased the whiteness and brightness as well as tensile strength and internal bond strength.

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Effect of Eleutherococcus senticosus supplementation on laccase and cellulase activity of Ganoderma lucidum (가시오가피 첨가가 영지버섯의 라카아제 및 셀룰라아제 효소 활성에 미치는 영향)

  • Kim, Hong-Il;Jeong, Yong-Un;Lee, Chang-Soo
    • Journal of Mushroom
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    • v.16 no.4
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    • pp.347-351
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    • 2018
  • The aim of this study is to investigate the effect of Eleutherococcus senticosus (ES) on the laccase and cellulase activity of Ganoderma lucidum mycelia. Following the addition of ES, the laccase activity of Ganoderma mushroom mycelia was found to be 0.84-2.18 times, 0.61-2.37 times, and 0.78-2.17 times the activity of mycelia treated with sawdust in Yeongji-1 (Y1; ASI-7004), Yeongji-2 (Y2; ASI-7071), and nokgak (GN; ASI-7013), respectively, with the laccase activity of Y2 being the highest at 0.947 U/min. Using the Congo-red assay, a wider clear zone was formed because of the CMCase activity of mycelia treated with ES than that of mycelia treated with sawdust. Cellulase activity was found to be 1.84-2.24 times, 1.77-1.87 times, and 2.74-2.81 times that of mycelia treated with sawdust in Y1, Y2, and GN, respectively, with the cellulase activity of GN being the highest at 0.172 U/min. However, the addition of ES did not affect the growth of G. lucidum mycelia.

Control of Catabolite Repression by Limit Feed of Cellobiose in Cellulomomas sp. (Cellulomonas sp.에 있어서 셀로비오스의 미량공급에 의한 생성물 저해의 조절)

  • Choi, Woo-Young
    • Korean Journal of Agricultural Science
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    • v.3 no.2
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    • pp.235-243
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    • 1976
  • As an investigation on the catabolite repression system in cellulase production by Cellulomons sp. CS1-1, the organism was tested on the avicel overlay plates containing glucose or cellobiose at a range of concentration and was grown in continuous culture vessel, supplied by cellobiose medium, aiming the enhanced production of extracellular CM-cellulase at low dilution rates. Product inhibition of cellulase action by cellobiose was also tested. The results obtained are: i) no inhibition of CM-cellulase was observed up to 10 mM(3.4mg/ml) cellobiose in the reaction mixture, however 30% inhibition was observed at 20mM and 55% at 50mM, ii) the tests of catabolite repression on the solid media were successful, and avicel degradation was markedly repressed by glucose or cellobiose, iii) at low concentrations of cellobiose, dilution rate 0.05 and $1.0hour^{-1}$, no significant increase was observed in the production of either intra or extracellular CM-cellulase.

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A case Study on One-Step Bio-treatment including cellulase (셀룰라아제를 포함한 One-Step Bio-treatment 연구 적용사례)

  • Kim, Moon-Jung;Yoon, Min-Sun;Lee, Jung-Ho
    • Proceedings of the Korean Society of Dyers and Finishers Conference
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    • 2012.03a
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    • pp.94-94
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    • 2012
  • 섬유산업은 원가 경쟁력 향상과 산업 고도화에 필요한 미래형 저에너지 염색가공 핵심 기술을 통한 고부가가치 섬유제품의 창출과 섬유산업의 선진화달성에 꾸준히 노력을 해 왔다. 염색가공업의 에너지 소비는 섬유산업에서 염색가공업이 연료 사용량의 77%, 전기사용량의 54%를 차지하여 섬유산업의 에너지절감을 위해서는 염색가공 공정에서의 에너지 절감이 가장 중요하다. 국내 염색가공 분야의 4백여 업체를 대상으로 실시한 애로 기술에 관한 설문 조사결과에 따르면 염색공업의 에너지 절약형 구조로의 전환을 위해서 가장 시급히 요구되는 우선기술 1순위는 에너지절약형 염색가공 공정기술로 에너지 절감의 필요성을 반영하고 있다. 효소(Enzyme)가 섬유산업에 도입되기 시작한 것은 면섬유의 호발에 아밀라제가 사용되기 시작하면서 부터이며, 최근 유럽선진국들의 강화된 환경규제와 눈부신 바이오테크놀러지(Biotechnology)의 발전에 기인하여 섬유산업에서 관심과 적용이 확대되고 있다. 섬유산업에서 효소적용의 장점은 효소 그 자체가 자연산물이기 때문에 생분해 되고 중성에 가까운 pH에서 반응하므로 처리액이 환경문제를 일으키지 않으며, 기질특이성을 가져 매우 선택적으로 반응하여 부반응으로 인한 섬유의 손상을 최소화 하는 효과를 들 수 있으며, 무엇보다 소량 저온반응으로 인한 에너지절감이 가장 큰 장점이라 할 수 있다. 실제 섬유산업에서는 전분호제를 제거하는 아밀라아제, 데님워싱 및 면섬유의 후가공에 사용하는 셀룰라아제, 표백 후 잔류하는 과산화수소를 제거하는 카탈라제 등을 적용하는 사례가 많아지고 있으며, 이 밖의 다양한 공정에 효소 이용연구가 활발히 진행되고 있다. 본 연구에서는 그 동안 섬유 업체에서 직물의 표면 잔털을 제거하여 매끄러운 외관과 선명한 색상을 재현하기위해 후가공으로 셀룰라아제를 이용했던 공정을 개선하여, 효소를 이용한 정련-Bio polishing-염색의 3공정을 1욕처리를 통해 기존 단독으로 진행되어진 기존제품과의 중량, 외관, 색상재현성, 정련성 등을 비교하여 에너지 절약형 공정기술의 효과를 극대화 해보았다.

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The physical properties of the cosmetic hydrogels affected by adding various celluloses (셀룰로오즈 첨가에 따른 미용용 하이드로겔의 물성 연구)

  • Byeon, Hong-Ju;Choi, Won-Seok;Lee, Hyang-Yeol
    • Journal of the Korean Applied Science and Technology
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    • v.35 no.3
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    • pp.702-708
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    • 2018
  • Hydrogels are natural polymer networks that can contain huge quantities of water and many cosmetical ingredients. Their hydrophilic functional groups creates a matrix, which allows high efficacy in delivering active ingredients into the skin. In industry, hydrating properties and strength of the hydrogels are of great interest in manufacturing hydrogel mask packs. We have used the cellulose in various forms such as powder, cotton fiber and cellulase treated cotton fiber to investigate the property changes of cellulose/hydrogel sheets. When 0.1% and 0.3% of cellulose powder were added to hydrogels, tensile strength of hydrogel sheets were decreased by 10% and 14% respectively. Vise versa, when 0.5 ~ 2 cm of cotton fibers were added, tensile strength of hydrogel sheets were significantly increased by about 20%. The hydrogels which contain cotton fibers also gave an excellent moisturizing effect. Especially cellolose/hydrogels containing cellulase-treated cotton fibers showed the best effect on retaining moisture content increasing upto 380% in comparison with the one containing untreated cotton as well as excellent dispersibility.

The Change of the Physical Properties of Rayon/Cotton Blend Fabrics Treated with Cellulase by Addition of Silicon (셀룰라아제 처리시 실리콘 첨가에 따른 레이온/면 혼방직물의 물성변화)

  • 이선화;송화순
    • Journal of the Korean Society of Clothing and Textiles
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    • v.22 no.8
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    • pp.1032-1042
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    • 1998
  • The purpose of this study is to measure, compare, and investigate the physical properties of rayon/cotton blend fabrics treated with cellulase and cellulase & specific degeneration silicon and to present basic data which is in development a value-added fabric. The results are as follows. The condition for the treatment of cellulase was at 55$^{\circ}C$, pH 4 and the weight loss increased as the concentration of cellulase and the treated time increased. On treatment with cellulase, the crossectional view & longitudinal view of fiber noticed remarkable crack as weight loss increased and tensile strength and elongation decreased, and pilling was enhanced remarkably. KOSHI was increased, NUMERI and FUKURAMI were decreased as weight loss increased. In the basic characteristic value of clothing wearning, shape stability and drapability were decreased, but air content was improved. On treatment with cellulase & silicon, the degree of damage in the crossectional view & longitudinal view of the fiber reduced. Tensile strength, elongation, moisture regain improved. KOSHI and FUKURAMI were reduced, NUMERI was improved rather than when it was treated with cellulase. Therefore handle was improved. In the basic characteristic value of the clothing wearing, shape stability, air content, drapability were improved.

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Kinetic Modeling of the Enzymatic Hydrolysis of $\alpha$-Cellulose at High Sugar Concentration (순수 섬유소에 대한 고농도 당화공정의 동력학적 모사)

  • 오경근;정용섭홍석인
    • KSBB Journal
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    • v.11 no.2
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    • pp.151-158
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    • 1996
  • For the effective ethanol fermentation, the high concentration of sugar as the substrate of microbial fermentation is required. The most important reason in the inefficient hydrolysis; the easy deactivation of enzyme by temperature or shear stress and the severe inhibition effects of its products. In our work, we comprehended the kinetic characteristics of cellulose and ${\beta}$-glucosidase in the progress of hydrolysis, and observed the potential inhibitory effects of the hydrolyzed products and the deactivation of enzymes. We also tried to present the kinetic model of enzymatic hydrolysis of cellulose, which is applicable to process at the high concentration of sugar. Cellulase and ,${\beta}$-glucosidase exhibit diverse kinetic behaviors. At a level of only 5g/$\ell$ of glucose, the ${\beta}$-glucosidase activity was reduced by more than 70%. This result means that ${\beta}$-glucosldase was the most severely inhibited by glucose. Also at l0g/$\ell$ of cellobiose, the cellulose lost approximately 70% of its activity. ${\beta}$-glucosldase was more sensitive to deactivation than cellulose by about 1.6 times. The comprehensive kinetic model in the range of confidence was obtained and the agreement between the model prediction and the experimental data was reasonably good, testifying to the validity of the model equations used and the associated parameters.

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Cellulase activity of Aspergilli distributed in South Korea(I) (한국산 Aspergilli의 셀룰라아제 활성에 관한 연구 1)

  • 이영녹;김낙정;서항원
    • Korean Journal of Microbiology
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    • v.14 no.3
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    • pp.105-116
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    • 1976
  • 410 strains which belong to the genus aspergillus were isolated from specimens collected throught South Korea, and 17 species nad 1 variety through the 13 species groups were identified according to Repaer-Fennell's classification key. Among them the species and variety unrecorded in Korea are as follows : A, giganiteus, A. pseudogloucus, A. spinulosus, A.ficuum, A.japonicus, A.flavus var. columnaris, A. flavipes.

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The Bio-Softness Finish of Tencel fabric(Part I) (텐셀직물의 바이오-유연가공에 의한 물성변화(제1보))

  • 김인영;오수민;송화순
    • Journal of the Korean Society of Clothing and Textiles
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    • v.23 no.1
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    • pp.14-21
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    • 1999
  • The Tencel fabrics were treated with bio-softness finish to improve softness. The change of the properties depending on the softner as well as cellulase treatment was investigated. The relative activity of cellulase for tencel was maximum ap pH 4-4.3 cellulase concentration 14-16% (o. w .f) treatment time 4 hour and liquor ratio 100: 1 The treatment of epoxy silicon softner improved in pilling whiteness dye absorption of Tencel fabric.

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