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http://dx.doi.org/10.14480/JM.2018.16.4.347

Effect of Eleutherococcus senticosus supplementation on laccase and cellulase activity of Ganoderma lucidum  

Kim, Hong-Il (Department of Integrated Biosciences, College of Biomedical and Health Science, Konkuk University)
Jeong, Yong-Un (Department of Integrated Biosciences, College of Biomedical and Health Science, Konkuk University)
Lee, Chang-Soo (Department of Integrated Biosciences, College of Biomedical and Health Science, Konkuk University)
Publication Information
Journal of Mushroom / v.16, no.4, 2018 , pp. 347-351 More about this Journal
Abstract
The aim of this study is to investigate the effect of Eleutherococcus senticosus (ES) on the laccase and cellulase activity of Ganoderma lucidum mycelia. Following the addition of ES, the laccase activity of Ganoderma mushroom mycelia was found to be 0.84-2.18 times, 0.61-2.37 times, and 0.78-2.17 times the activity of mycelia treated with sawdust in Yeongji-1 (Y1; ASI-7004), Yeongji-2 (Y2; ASI-7071), and nokgak (GN; ASI-7013), respectively, with the laccase activity of Y2 being the highest at 0.947 U/min. Using the Congo-red assay, a wider clear zone was formed because of the CMCase activity of mycelia treated with ES than that of mycelia treated with sawdust. Cellulase activity was found to be 1.84-2.24 times, 1.77-1.87 times, and 2.74-2.81 times that of mycelia treated with sawdust in Y1, Y2, and GN, respectively, with the cellulase activity of GN being the highest at 0.172 U/min. However, the addition of ES did not affect the growth of G. lucidum mycelia.
Keywords
Eleutherococcus senticosus; lignocellulolytic enzyme; laccase; cellulase; Ganoderma lucidum;
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