• Journal of Life Science
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• v.21 no.12
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• pp.1752-1760
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• 2011

In this study, the effect of the Opuntiahumifusa extracts on proliferation, alkaline phosphatase (ALP) activity, collagen synthesis and ROS level of a cell was investigated using an osteoblast. Opuntiahumifusawas separated intoOpuntiahumifusapeel (OH-P), seed (OH-Se) and stem (OH-St).These were subjected to extraction by using hot water and ethanol. The proliferation of the MC3T3-E1 osteoblastic cells that were treated with OH-Se water extract were increased by approximately 120%. Regarding the effects of OH-Se on ALP activity, the $50{\mu}g/ml$ ethanol extract group showed the highest activity. The synthesis of collagen increased significantly in response to treatment with OH-Se water extract. The ROS scavenging effects of Opuntiahumifusawere investigated for involvement of oxidativedamage, cell culture and staining. Also, when OH-Se water extract $100{\mu}g/ml$ was added, the ROS level decreased by 54%. These results indicate that Opuntiahumifusa extracts have an anabolic effect on bone through the promotion of osteoblastic differentiation, suggesting that it could be used for the treatment of common metabolic bone diseases.

• KSBB Journal
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• v.8 no.5
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• pp.446-451
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• 1993

In ethanol fermentation, by-products such as glycerol, acetic acid and lactic acid are produced along with ethanol. The effects of culture conditions on cell growth ethanol production and by-products biosynthesis were investigated in ethanol fermentation using S. cerevisiae. With increasing aeration rate or yeast extract concentration, ethanol and by-products biosynthesis decreased while final cell mass increased. With increasing glucose concentration or decreasing temperature, final cell mass, ethanol and by-products concentrations all increased. The optimal pH for the cell growth, ethanol and by-products productions was found to be pH 4.5. By-products biosynthesis was found, in general, to proceed with the ethanol biosynthesis. The results can be applied for the optimization of ethanol fermentation and for the recovery and purification of ethanol from the culture broth.

• Korean Journal of Medicinal Crop Science
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• v.13 no.4
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• pp.161-170
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• 2005

This study was performed to examine immuno-regulatory activities of Ehpedrae Sinica STAPF, Rubus Coreanus Miq. and Angelica gigas Nakai extracts in conbination with ultrasonification. The extract yields of plants were the highest in the extraction system of $60^{\circ}C$ and 40 kHz of ultrasonification. The immune cell growth ratio of human immune B and T cells was increased compared to other fractions by the water fraction of the plants at $60^{\circ}C$ and 40 kHz. The water fractions of the plants at $60^{\circ}C$ and 40 kHz increased the specific secretion of IL-6 and $TNF-{\alpha}$ of human immune B and T cells compared to other fractions of the plants. The water fraction of Ehpedrae Sinica STAPF among the plants was observed to show the highest specific secretion of IL-6 and $TNF-{\alpha}$. Also, NK-92 MI cells growth was increased in adding the water fractions of the plants at $60^{\circ}C$ and 40 kHz. The water fraction of Ehpedrae Sinica STAPF among the plants showed the highest in NK-92 MI cell growth ratio. The differentiation activity of the HL-60 cells significantly increased in adding the water fraction of Ehpedrae Sinica STAPF compared to other fractions of the plants. These results suggest that the water fractions of the plants in extraction system of temperature $60^{\circ}C$ and ultrasonification 40 kHz have marked useful immuno-stimulatory activities.

• Korean Journal of Medicinal Crop Science
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• v.15 no.6
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• pp.411-416
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• 2007

This study was performed to investigate the reduction of toxicity and improvement of anticancer activities from R. sachalinensis by ultra high pressure extracts process. The cytotoxicity on human kidney cell (HEK293) and human lung cell (HEL299) was showed below 20.4% and 21.6% as compare to normal extracts in adding 1.0 $mg/m{\ell}$ concentration. This showed that toxic materials through ultra high pressure processing is broken or degraded. Because bond such as hydrogen bond, electrostatic bond, Van der waals bond, the hydrophobic bond, can be broken by high pressure. The anticancer activity was also increased in over 7% by high pressure processing in A549, AGS, MCF-7 and Hep3B cells. The result showed that extraction by high pressure have low cytotoxicity and high anticancer activity. So, the high pressure extraction technology can play an important role in eruption of new material with high biological activity.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.376-379
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• 2000

The methylotrophic yeast Pichia pastoris is one of the best host for the production of foreign proteins because of the presence of the strong AOX1 promoter induced by methanol. Methanol feeding induces the protein production and provides energy sources for the host cells. However, excess methanol inhibits the growth of host cells, while an insufficient methanol lead to poor growth and protein production. We have used various controled methanol feeding strategies to obtain the maximum proteins.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2004.05a
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• pp.373-375
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• 2004

본 연구는 유황오리로부터 항종양 효과를 나타내는 물질을 용매추출과 각종 chromatography를 사용하여 분리 및 정제하였다. HPLC를 사용하여 정제한 항종양활성 성분의 수율은 유황오리 1Kg당 10mg이었다. HPLC를 이용하여 정제한 항종양활성 물질을 Clonogenic assay로 측정한 결과 $200{\mu}g/m{\ell}$의 농도에서 HEp-2, KB 및 SW-156에서 각각 26%, 28%및 30%의 생존율을 나타내어 항종양 효과가 나타났다. MTT assay에서는 $100{\mu}g/m{\ell}$의 농도는 Farrow, HEp-2 및 KB에 대해서 각각 56%, 58% 및 56%의 세포증식 억제 효과를 나타내었다. bovine normal kidney cell line인 MDBK는 $500{\mu}g/m{\ell}$, $200{\mu}g/m{\ell}$, $100{\mu}g/m{\ell}$에서 각각 48%, 34%, 28%로 증식억제 효과가 나타나 정상세포에 대한 세포독성은 강하지 않은 것으로 판단되어진다.

• Korean Journal of Food Science and Technology
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• v.25 no.1
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• pp.78-82
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• 1993

This study was carried out to develop a new processing method for the production of yeast autolyzate (YA) by which the yield and the quality could be improved, compared to the conventional method. The result indicated that 85.3% of yeast cell walls treated with glucanase and protease was ruptured by homogenizing at 10,000 psi. Alkali treatment, however, was not effective in disintegrating yeast cell walls. Optimum conditions for autolysis of ruptured yeast cells, obtained with help of response surface methodology (RSM), were pH 7.0, $30^{\circ}C$, ethanol 4% and salt 3%. YA produced by the developed method had significantly higher yield and better sensory quality than that produced by the conventional method.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.15 no.5
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• pp.387-395
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• 2022

PDRN (Polydeoxyribonucleotide) is a DNA-derived polymer that promotes self-renewal of damaged cells and tissues as a tissue regeneration active material. PDRN is a DNA fragment cut into small sizes by various physical or chemical methods. When administered to the body, PDRN binds and stimulates the adenosine A2A receptor on the surface of tissue cells to promote cell regeneration, accelerate wound healing, and reduce pain. Although PDRN is prepared from testis or semen of fish in most cass, PDRN extraction from various plants species was performed in the present study. Among 7 tested plant species, the highest DNA yield and purity was obtained form mugwort (Chrysanthemum coronarium, C.c), followed by broccoli (Brassica oleracea, B.o). Then, we evaluated the in vitro wound healing capacity of PDRNs prepared from these two selected plants. PDRN from C.c and B.o. significantly stimulated the wound healing process at ㎍/ml range. The present study suggests that PDRN from plant species can be an effective alternative to PDRN from marine organism.

• Microbiology and Biotechnology Letters
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• v.30 no.2
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• pp.167-171
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• 2002

The resting cells of Candida sp. SY16 produced a large amount of mannosylerythritol lipid as a biosurfactant when incubated in the distilled water containing only the carbon source. The resting cells exhibited the highest production at 20 g cells per liter on the soybean oil of 75 g/1 as a sole substrate and pH 4∼5 in the shaking culture. Under the optimal conditions, the biosurfactant was extracellularly produced to 58 g/1 after 120 h in jar fermentor, and the yield became higher than that obtained by using the glowing cells of the strain in batch fermentation.

• KSBB Journal
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• v.7 no.1
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• pp.79-83
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• 1992

The characteristics of Erwinia rhapontici cells with $\alpha$-glucosyltransferase activity immobilized in Ca-alginate beads and the performance of two different types of reactor-stirred tank reactor(STR) and packed bed reactor(PBR)-charged with these immobilized cells to produce palatinose from sucrose were investigated. The optimal pH(5.5-6.0) and temperature($30-35^{\circ}C$) showed no appreciable difference between free and immobilized cells. The apparent Km value of the immobilized cells(0.28M) was approximately two times higher than that of free cells(0.13M) at $30^{\circ}C$. The half life of the immobilized cells was found to be 380 h with STR while much greater operational stability was achieved with PBR. Continuous operation of PBR at a space velocity of $0.2h^{-1}$ for 30 days showed only 5% loss of initial activity.