Experiment was conducted to get the information about physico-chemical properties of flue-cured tobacco on the degree of maturity cultivated in paddy-upland rotated field, and compared to upland ones. For the samples of this experiment, 3~4 leaves at each stalk position were harvested from the bottom of plants. Physico-chemical properties of cured leaves were determined from the samples collected at weekly intervals, and of obtained from 4 stalk positions. For the degree of maturity, harvested leaves were separated with visual characters into four classes such as immature, mature, ripe, and mellow. Regardless of stalk position, the order of shrinkage rate with length and width of leaves was mellow> immature> ripe> mature, and ripe leaves from paddy field showed higher shrinkage rate than those of upland. Nicotine and total nitrogen contents were decreased with the degree of maturity while reducing sugar content were showed a reverse tendency. Ripe leaves from paddy field had lower reducing sugar contents, comparing with upland tobacco. Filling capacity of cured leaves from paddy field was decreased with degree of maturity, but there was no difference between upland and paddy tobacco. Shatter index was increased in the oeder of immature > mellow > mature > ripe. Chemical components of cigarette smoke from paddy field tobacco were little higher in $CO_2$ total particulate matter and tar contents, while combustibility was little lower than that of upland tobacco. It was also evaluated that paddy field tobacco was unfavorable for the non-volatile organic and higher fatty acids contents comparing with upland tobacco.
To evaluate whether mast cells are involved in developing pathologic feature of psoriasis, 60 biopsy specimens of patients with psoriasis were analysed. They had not been treated for at least 1 week before skin biopsy. Histological findings in early and fully developed lesions and numbers of mast cells in their dermal papillae were investigated. The result were as follows : 1. In epidermal changes of psoriatic lesions, parakeratosis and acanthosis revealed different findings between early lesions and fully developed lesions. While early lesions revealed mounds of parakeratosis and mild to moderate acanthosis, fully developed lesions revealed confluent parakeratosis and moderate to severe acanthosis. In dermal changes of psoriatic lesions, papillomatosis revealed different findings between early developed lesions and fully developed lesions. While early lesions revealed normal to mode-rate papillomatosis, fully developed lesions revealed moderate to severe papillomatosis. 2 Degree of acanthosis is related to the degree of papillomatosis. The more increase in the degree of acanthosis, papillomatosis, and parakeratosis, mast cell numbers in dermal papillae were more increased. 3. Mast cell numbers in dermal papillae were more increased in fully developed lesions than early lesions. 4. These findings suggest that mast cell may play an active role in developing pathologic finding of psoriasis.
The objective of this experiment was to test the ability of the fertilization of EGF treated pig oocytes for in vitro maturation. The addition of EGF (10 ng/ml), FSH (10 ${\mu}\textrm{g}$/ml), or FBS (10%) on maturation medium of pig immature oocytes divided into four groups as follows; group 1: untreatment, group 2: EGF alone, group 3: combination of FSH and FBS, or group 4: combination of EGF, FSH, and FBS. The interactive effects of nuclear maturation rates (M II%) of EGF alone, FSH plus FBS, and EGF plus FSH added FBS treatments were significantly higher than those of non-treatments (P<0.001). The fertilization rate of EGF alone (group 2) was lower than that of 3, 4 groups, but was significantly higher than group 1 (p< 0.005). Furthermore, combination of EGF, FSH,and FBS (group 4) was higher than others (group 1. 2, 3) on male pronuclei formation as well as penetration of sperm (P<0.05). These results suggested that EGF alone decreased the ability of cytoplasmic maturation compared to nuclear maturation in pig oocytes, but a high level of cytoplasmic maturation of in vitro-matured pig oocytes can be achieved when supplemented with FSH and FBS.
Objective: To estimate dental maturity using the Demirjian Index for the mandibular third molar and investigate the relationships between dental maturity and skeletal maturity among growing children. Methods: The samples were derived from panoramic, lateral cephalometric and hand-wrist radiographs of 270 female subjects registered as patients at the orthodontic department, dental hospital, Yonsei University. Dental maturity [Demirjian Index (DI)] and skeletal maturity [skeletal maturation indicators (SMIs)] and cervical vertebrae maturation indicators (CVMIs)] were estimated from these radiographs. Results: There was a significant correlation (r = 0.64) between SMIs and DI, and a similar correlation (r = 0.59) was observed between CVMIs and DI (p < 0.001). If DI was above Stage E, then the SMI was above Stage 10 and the CVMI was above Stage 5. There was a weak correlation (r = 0.26) between age at menarche and DI (p < 0.001). There was no significant difference in DI among Class I, II or III malocclusions. Conclusions: Dental maturity evaluation using the mandibular third molar will be an adjunctive tool in combination with cervical vertebrae and hand-wrist maturity evaluations.
To estimate the spawning period the annual change of gonadosomatic index (GSI) were examined from January 2000 to December 2001. Fecundity, spawning frequency and egg diameter were measured by ocular observation. Germ cell differentiation during gametogenesis, the reproductive cycle and the first sexual maturity of greenling Hexagrammos otakii were observed under light microscopy from January to December, 2000. GSI began to increase in August and reached the maximum in November when ovary was getting mature. The reproductive cycle of H. otakii can be divided into five successive stages in females: early growing stage (July), late growing stage (July to August), mature stage (September to October), ripe and spent stage (September to December), and recovery and resting stage (December to June). Males showed four successive stages : growing (June to August), mature (August to October), ripe and spent (September to December), and recovery and resting stage (December to May). According to the frequency distributions of egg diameter in spawning season, H. otakii could be one of polycyclic species spawning 2 times or more during one spawning season. Number of total eggs and mature eggs in the absolute fecundity were related to the standard length and body weight, respectively. Number of total eggs and mature eggs in relative fecundity were also proportional to the standard length, but rather these numbers decreased with body weight. Percentages of first sexual maturity of females and males in greenling were over 50% from 19.1 to 21.1cm in length, and 100% for fish over 25.1cm in length. Therefore, both sexes are ready to reproduce after one year old.
Kim, Byung-Ki;Kang, Sung-Ryoung;Yim, Cha-Ok;Han, Chang-Hee;Lee, Bok-Kyu;Lee, Kwang-Sun
Journal of Life Science
/
v.13
no.5
/
pp.732-739
/
2003
The purpose of this study was to determine if the addition of spermatozoa into the culture medium could influence the nuclear maturation of denuded porcine germinal vesicle (GV) oocytes in vitro. Cumulus-oocyte complexes were collected from follicles of 3 to 5 mm in diameter, The cumulus and corona cells were removed from oocytes. Porcine denuded oocytes were cultured in tissue culture medium containing spermatozoa. After 48 h culture, oocytes were examined for the evidence of GV breakdown, metaphase I, anaphase-telophase I, and metaphase II (M II). The proportion of oocytes reaching M II stage was significantly (P<0.01) increased in the oocytes cultured in media containing spermatozoa compared to those in media without spermatozoa $(31.9\pm1.8%\; vs\; 14.9\pm1.0%)$.No differences in the rates of M II were observed among the different period of spermatozoa exposure nor among the spermatozoa from different species. The proportion of oocytes reaching M II stage was significantly different between high and low concentrations of spermatozoa. The present study suggests that mammalian spermatozoa contain a substance(s) that improves nuclear maturation in vitro of GV oocytes. Enhancing effect of spermatozoa for oocytes maturation in vitro is a highly dose-dependent.
Journal of the Korean Applied Science and Technology
/
v.35
no.2
/
pp.454-462
/
2018
This study is a descriptive research study to examine influence of major satisfaction and career maturity on the job-seeking stress of nursing and public health university students. This study surveyed 410 nursing and public health university students from 3 schools of G-city from September 26 to October 31, 2017. The data were analyzed by descriptive statistics, ANOVA, ANCOVA, Pearson correlation coefficients, and multiple regression. The study results showed a significant difference in major satisfaction (F=9.856, p<.001) and career maturity (F=6.005, p=.001) according to major choice motives. The student GPAs were also found to be significantly different from career maturity (F=2.755, p=.042) and job-seeking stress (F=3.503, p=.016). In addition, the TOEIC score was significantly different from major satisfaction (F=4.514, p=.004). Job-seeking stress was positively correlated with major satisfaction (r=-.314, p<.001) and career maturity (r=-.514, p<.001). The factors affecting job-seeking stress were in the order of major satisfaction (${\beta}=-.125$, p=.007) and career maturity (${\beta}=-.459$, p<.001). Based on the results, two proposals are recommended in order to reduce the job-seeking stress of nursing and public health university students. It is necessary to prepare major guidance programs suitable to the characteristics of each department from the lower grades and to provide the students with career guidance accordingly.
Journal of the Korea Academia-Industrial cooperation Society
/
v.20
no.12
/
pp.525-534
/
2019
The study was carried out by surveying 500 fostered teenagers at youth shelters in the Capital area via onsite visit over about three weeks from April 17 to May 10, 2018; 487 samples out of 500 questioned subjects were used for the final analysis. The results of this study are as follows. First, the career decision self-efficacy was verified to have a partial mediating effect between all of self-leadership variables and the career maturity of the fostered adolescents at youth shelters. Second, the career decision self-efficacy was verified to have a partial mediating effect between the action-oriented strategy, which is one of the self-leadership variables, and the career maturity of the fostered adolescents at youth shelters. Third, the career decision self-efficacy was verified to have a partial mediating effect between the natural compensation strategy, which is one of the self-leadership variables, and the career maturity of the fostered adolescents at youth shelters. Fourth, the career decision self-efficacy was verified to have a full mediating effect between the constructive thinking strategy, which is one of the self-leadership variables, and the career maturity of the fostered adolescents at youth shelters.
The present experiments were carried out to find out the proper concentration of CEPA(2-chloroethy-lphosphonic acid) for the purpose of accelerating the leaf maturity and of reducing the nicotine content of tobacco. Varried levels of CEPA were sprayed Yellow Special A right after topping. The effects of each level CEPA treatment on leaf ripening and total alkaloid contents were periodically observed. The results are summarized as follows; 1. The higher the CEPA concentration was, the more the leaf maturity was accelerated. During the period from six to 11 days after treatment, the differences of leaf maturity among levels were prominent. 2. Treatment with CEPA only on the upper surface of the tobacco leaf, accelerated the maturity of that particular part treated, but not apparently the other parts of leaf. 3. The higher the CEPA concentration was, the more the accumulation of the total alkaloid was reduced. The reduction of alkaloid accumulation was evident after acceleration of leaf maturity. 4. Distinctive acceleration of leaf maturity was observed in the fully developed lower leaves, while reduction of alkaloid accumulation in the growing upper leaves. The degree of total alkaloid content reduced in the ripened leaves, however, were similer to all the leaves at different positions. 5. Yields of tobacco leaves were not significantly affected by CEPA treatment. 6. In the present experiments, it may be concluded that CEPA2, 000ppm is the most applicable level for accelerating leaf maturity and decreasing total alkaloid content. In the view point of the practical use, however, the applicable level is assumed to be properly choosed between 500 to 2, 000ppm, depending on the situations. 7. The mechanism of accelerating maturity and reducing alkaloid accumulation of tobacco leaves by CEPA, is further to be explored.
In order to classify the ecotypical and maturing groups 136 Korean local and 4 Japanese Perilla varieties were sown at Suwon on May 20, 1972, and investigations were made on days to flowering, days to maturing and growth duration. Results obtained were summarized as follows: 1. Ecotype was classified from 6 groups with combination of the long and short duration from sowing to flowering and maturing period. The classified symbols of groups were Ia, Ib, IIa, IIIa, and IIIb. I: long duration. to flowering II: medium duration to flowering III: short duration to flowering a: long duration to maturing b: short duration to maturing Korean local varieties were distributed 3 ecotypes of IIa, IIb and IIIa, however, Japanese varieties did 2 ecotypes of IIa and IIb, respectively. 2. Maturing group was classified from 3 groups with long (I), medium (II), and short (III) growth durations. Korean local varieties belong to II and III groups but Japanese varieties belong to I and II groups, respectively. 3. Three were highly significant positive correlation (0.685) between ecotypical and maturing group. Ecotype was more related with days to flowering and growth duration, however, maturing group was related with days to flowering, days for maturing, and growth duration. Korean local varieties, more distributed into ecotype than maturing group, were attributed to differences of environmental conditions and cultural practices at different provinces.
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