• Title/Summary/Keyword: 선별공정

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Enhancement of L-lysine Productivity by Strain Improvement and Optimization of Fermentation Conditions in Corynebacterium glutamicum (Corynebacterium glutamicum 균주 개량 및 발효 공정 최적화에 의한 L-lysine 생산성 증진)

  • Seo, Jin-Mi;Hyun, Hyung-Hwan
    • KSBB Journal
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    • v.21 no.2
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    • pp.79-84
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    • 2006
  • In order to minimize the reduction of lysine productivity by accumulation of lysine and byproducts in the end of fed-batch fermentations, a salt-tolerant mutant C14-49-3-15-7-3-20, which could grow at high concentrations of NaCl was isolated through mutagenesis from the Corynebacterium glutamicum mother strain I. In the evaluation of L-lysine productivity by fed-batch fermentations using a 5 L jar fermenter, the salt-tolerant mutant strain C14-49-3-15-7-3-20 produced 130.6 g/L of L-lysine with a 48.6% of yield. The mother strain I produced L-lysine concentration only 104.9 g/L with a yield 41.8%, implying the improvement of L-lysine productivity by introduction of salt-tolerance character.

Breakage and Liberation Characteristics of Iron Ore from Shinyemi Mine by Ball Mill (신예미 광산 철광석의 볼밀 분쇄 및 단체분리 특성 연구)

  • Lee, Donwoo;Kwon, Jihoe;Kim, Kwanho;Cho, Heechan
    • Resources Recycling
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    • v.29 no.3
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    • pp.11-23
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    • 2020
  • This study aims to investigate breakage and liberation characteristics of iron ore from Shinyemi mine, Jeongseon by ball mill. Parameters of breakage functions for three grade samples of iron ore were obtained using single-sized-feed breakage test and back-calculation based on nonlinear programming. The results showed that with the increase in the grade of iron ore, the breakage rate factor decrease whereas the particle size sensitivity decreases. This results from retardation of microcrack-propagation by magnetite grain in the ore. Breakage distribution analysis showed that the breakage mechanism appear to be impact fracture dominant with the increase of grade owing to the stress distribution effect by magnetite grain. Degree of liberation (DOL) increased with the increase in grade and decrease in particle size, respectively. Using the breakage function and size-DOL relationship, a model that can predict time-dependent-DOL is established. When scale-up factors from operating condition are available, the model is expected to be capable of predicting size and DOL with time in actual mining process.

The Recycling of Sludge from Granite Stone Cutting and Polishing (화강암 석재 가공 슬러지의 재활용)

  • 이성오;국남표;임영빈;신방섭
    • Resources Recycling
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    • v.4 no.1
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    • pp.12-19
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    • 1995
  • This study was carried out to remove the iron and impurities usmg hydrocyclone and HGMS for recycling of sludge from the granite stone cutting and polishing industrγ in the basic of chemi떠1 analysis and minerallogical investigation. This sludge consist of 70.9% $SiO_2$ 13.6% $Al_2O_3$ and It also contained 2.52% of $Fe_2O_7$ and 0.29% of $TiO_2$, as a main impurities to decrease the whiteness. As the result of hydrocyclone experiment, It was very good condition that are 100~150 g/l of sludge amount, 2.0~ 2.5 mm of underflow nozzle size, and 1.2~1.6 kg/$\textrm{cm}^2$ of pressure for 85% sludge product with the $-37{\mu}\textrm{m}$ size. $Fe_2O_3$ and $TiO_2$, contents by treatment of HGMS were decreased with 0.65% and 0.07% each at 10,000 gauss of magnetic field strength, and addih$\upsilon$n of Sodium tripolyphosphate as a dispersant was effected to get low grade F Fe,Ol and TiO, concentrate. PhYSIcal properties of this stone sludge product were showed 58.5% of whiteness, 1 13.4% of firing shrinkage and 3.0812 $\textrm{m}^2$/g of specific surface area.

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Does the Quantitative Supply of Secondary Teachers Compromise their Quality?: A Comparative Analysis of South Korea and China (한국과 중국의 중등학교 교사 신규채용제도 비교 분석)

  • Kim, Ee-Gyeong;Ahn, Ji-Yoon;Wei, Hua-Ou
    • Korean Journal of Comparative Education
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    • v.24 no.3
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    • pp.157-177
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    • 2014
  • The importance of selection methods of secondary school teachers have gathered attention due to their enormous influence on the pre-service education programs. However, scholars and practitioners are skeptical about the effectiveness of the measures, especially under the condition of teacher oversupply found in South Korea and China. Based on this problem statement, this study seeks to compare the secondary teacher employment methods of the two countries to better understand the situations as well as to draw implications for improvement. We reach the following conclusions through comparative analyses. First, the reforms of the teacher employment system in both countries have been triggered by the quantitative supply concerns not by the qualitative concerns. Second, both countries require teaching license as a precondition of employment, while their quality assurance measures for the license reveal critical limitations. Third, both countries have attempted to improve selection methods; nevertheless, oversupply of prospective teachers makes objectivity and fairness more important than the appropriateness. Based on the conclusions, policy implications focusing on the quality-oriented approach are suggested in order to employ effective secondary teachers who can contribute to the quality improvement of school education.

Compressive Strength Evaluation of Concrete with Mixed Plastic Waste Aggregates Filled with Blast Furnace Slag Fine Powder (무기충진재를 혼입한 복합 폐플라스틱 골재를 활용한 콘크리트 압축강도 특성)

  • Lee, Jun;Kim, Kyung-Min;Cho, Young-Keun;Kim, Ho-Kyu;Kim, Young-Wook
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.9 no.3
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    • pp.253-259
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    • 2021
  • Plastic wastes generated from household waste are separated by mixed discharge with foreign substances, and recycling is relatively low. In this study, the effect of the ratio and content of mixed plastic waste coarse aggregate(MPWCA)s and mixed plastic waste fine aggregate(MPWFA)s filled with blast furnace slag fine powder on the slump and compressive strength of concrete was evaluated experimentally. The MPWCAs were found to have a similar fineness modulus, but have a single particle size distribution with a smaller particle size compared to coarse aggregates. However, the MPWFAs were found to have a single particle size distribution with a larger fineness modulus and particle size compared to fine aggregates. Meanwhile, the effect of improving the density and filling pores by the blast furnace slag fine power was found to be greater in the MPWFA compared to the MPWCA. As the amount of the mixed plastic waste aggregate(MPWA)s increased, the slump and compressive strength of concrete decreased. In particular, the lower the slump and compressive strength of concrete was found to decrease the greater the amount of MPWFA than MPWCA when the amount of MPWA was the same. This is because of the entrapped air and voids formed under the angular- and ROD-shaped aggregates among the MPWFAs. On the other hand, the addition of the admixture and the increase in the unit amount of cement were found to be effective in improving the compressive strength of the concrete with MPWAs.

Distribution and Evaluation of Rare Earth Elements contained in Coal Ashes from Korea Circulating Fluidized Bed Combustion (CFBC) (국내 순환유동층보일러 석탄재의 희토류 분포 특성 및 평가)

  • Kim, Young-Jin;Baek, Chul-Seoung;Seo, Jun-Hyung;Choi, Moon-Kwan;Cho, Kye-Hong;Ahn, Ji-Whan
    • Resources Recycling
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    • v.27 no.6
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    • pp.68-75
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    • 2018
  • The rare earth elements (REE) contents in coal ashes generated from domestic circulating fluidized bed combustion (CFBC) were identified for evaluating the exploitation possibilities for recovering rare earth elements. Total REE contents for all of the samples in this study ranged from 82.2 ~ 311.7 ppm, much lower than the 403.5 ppm given on the average value of world coal ash. As a result of analysis using REE concentration and Outlook coefficient, six types of coal ashes falls in the unpromising area (I). These results suggest that it is difficult to recover rare earth element from coal ashes at this stage. It has been confirmed that to recover rare earth elements in coal ashes, research on the pretreatment and concentration process for critical REE is requirement.

Adhesion control of Campylobacter jejuni in chicken skin using emulsifiers (유화제를 이용한 계육 표면에서 Campylobacter jejuni의 부착 제어)

  • Oh, Do Geon;Kim, Kwang Yup
    • Korean Journal of Food Science and Technology
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    • v.52 no.6
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    • pp.670-677
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    • 2020
  • To prevent contamination by Campylobacter jejuni during chicken carcass processing, the effect of emulsifiers on C. jejuni inoculated on chicken skin was investigated using confocal laser scanning microscopy. Among the 8 emulsifiers (SWA-10D, L-7D, M-7D, S-1670, L-1695, P-1670, polysorbate 20, polysorbate 80) tested for antimicrobial activity by the paper disk method, 4 emulsifiers (L-7D, L-1695, polysorbate 20, polysorbate 80) were screened further. Emulsifier L-1695 showed the largest clear zone at a concentration of 200 mg/mL. The 4 emulsifiers subjected to primary screening were screened for heat and pH stability. In the contact surface test, emulsifier L-1695 showed the lowest log CFU/㎠ value on both stainless steel and ceramic surfaces. When emulsifier L-1695 was applied via general and electrostatic spray methods, the number of C. jejuni entrapped inside chicken skin follicles was significantly reduced in both methods. In conclusion, the emulsifier L-1695 could be employed as a microbial detachment agent in the chicken carcass processing industry.

Recent Advances in the Biotechnological Production of Natural Vanillin (생물공학에 기반한 천연 바닐린 생산에 관한 최근 연구)

  • Kim, Hyun-Song;Kim, Young-Ok;Lee, Jin-Ho
    • Journal of Life Science
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    • v.31 no.11
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    • pp.1046-1055
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    • 2021
  • Vanillin is the primary flavor and fragrance compound of natural vanilla and is extensively used in the food, beverage, perfumery, pharmaceutical industries, and other applications. Vanillin can be produced by chemical synthesis, extraction from vanilla plants, microbial bioconversion of natural precursors to vanillin, and direct fermentation using glucose. Currently, most commercially available vanillin is produced by extraction from cured vanilla pods and by chemical synthesis using guaiacol and glyoxylic acid as starting raw materials. Due to environmental issues, health complaints, preference for natural sources, and the limited supply and soaring price of natural vanilla, biotechnology-based vanillin production is regarded as a promising alternative. As many microorganisms that are able to metabolize several natural precursors, including ferulic acid, eugenol, isoeugenol, and lignin, and accumulate vanillin, have been screened and evaluated, myriad strategies and efforts have been employed for the development of commercially viable production technology. This review outlines the recent advances in the biotechnological production of natural vanillin with the use of these natural precursors. Moreover, it highlights the recent engineering approaches for the production of natural vanillin from renewable carbon sources based on the de novo biosynthetic pathway of vanillin from glucose, together with appropriate solution strategies to overcome the challenges posed to increase production titers.

A Study on Phosphate Removal Characteristic of EAF Slag for Submarine Cover Material (EAF Slag의 해양복토제 활용을 위한$PO_4{^-}-P$ 제거특성에 관한 연구)

  • Kim, Jae-Won;Seo, Jong-Beom;Kang, Min-Gyeong;Kim, In-Deuk;Oh, Kwang-Joong
    • Clean Technology
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    • v.16 no.4
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    • pp.258-264
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    • 2010
  • This study estimated the possibility of phosphate removal characteristics to utilize EAF(electric arc furnace) slag as submarine cover material. The major phosphate removal mechanism was a certain formation of HAP precipitation occurred by the ionization reaction between $Ca^{2+}$ and $OH^-$, which were leached from the EAF Slag. Another phosphate removal mechanism was the adsortion of EAF slag surface. As a result of $PO_4{^-}-P$ removal characteristics using continuous column reactor, $PO_4{^-}-P$ concentration decreased rapidly after 3 days and 10 days later, it show under 0.5 ppm. The result as applied in real sea water, shows that the phosphate removal effects were 93~98% by the subaqueous sediment removal using the EAF slag. In conclusion, EAF slag is useful in $PO_4{^-}-P$ removal and control and it is possible to use without additional process like crush and selection.

Performance Evaluation of Loss Functions and Composition Methods of Log-scale Train Data for Supervised Learning of Neural Network (신경 망의 지도 학습을 위한 로그 간격의 학습 자료 구성 방식과 손실 함수의 성능 평가)

  • Donggyu Song;Seheon Ko;Hyomin Lee
    • Korean Chemical Engineering Research
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    • v.61 no.3
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    • pp.388-393
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    • 2023
  • The analysis of engineering data using neural network based on supervised learning has been utilized in various engineering fields such as optimization of chemical engineering process, concentration prediction of particulate matter pollution, prediction of thermodynamic phase equilibria, and prediction of physical properties for transport phenomena system. The supervised learning requires training data, and the performance of the supervised learning is affected by the composition and the configurations of the given training data. Among the frequently observed engineering data, the data is given in log-scale such as length of DNA, concentration of analytes, etc. In this study, for widely distributed log-scaled training data of virtual 100×100 images, available loss functions were quantitatively evaluated in terms of (i) confusion matrix, (ii) maximum relative error and (iii) mean relative error. As a result, the loss functions of mean-absolute-percentage-error and mean-squared-logarithmic-error were the optimal functions for the log-scaled training data. Furthermore, we figured out that uniformly selected training data lead to the best prediction performance. The optimal loss functions and method for how to compose training data studied in this work would be applied to engineering problems such as evaluating DNA length, analyzing biomolecules, predicting concentration of colloidal suspension.