• Science of Emotion and Sensibility
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• v.14 no.4
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• pp.503-512
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• 2011

Recently in HCI research, emotion recognition is one of the core processes to implement emotional intelligence. There are many studies using bio signals in order to recognize human emotions, but it has been done merely for the basic emotions and very few exists for the other emotions. The purpose of present study is to confirm the difference of autonomic nervous system (ANS) response in three emotions (boredom, pain, and surprise). There were totally 217 of participants (male 96, female 121), we presented audio-visual stimulus to induce boredom and surprise, and pressure by using the sphygmomanometer for pain. During presented emotional stimuli, we measured electrodermal activity (EDA), skin temperature (SKT), electrocardiac activity (ECG) and photoplethysmography (PPG), besides; we required them to classify their present emotion and its intensity according to the emotion assessment scale. As the results of emotional stimulus evaluation, emotional stimulus which we used was shown to mean 92.5% of relevance and 5.43 of efficiency; this inferred that each emotional stimulus caused its own emotion quite effectively. When we analyzed the results of the ANS response which had been measured, we ascertained the significant difference between the baseline and emotional state on skin conductance response, SKT, heart rate, low frequency and blood volume pulse amplitude. In addition, the ANS response caused by each emotion had significant differences among the emotions. These results can probably be able to use to extend the emotion theory and develop the algorithm in recognition of three kinds of emotions (boredom, surprise, and pain) by response measurement indicators and be used to make applications for differentiating various human emotions in computer system.

• Journal of Pharmaceutical Investigation
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• v.39 no.6
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• pp.393-400
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• 2009

PHEA (hydroxyethyl-aspartamide)-mPEG (methoy-polyethyleneglycol)-$C_{16}$ (hexadecylamine)-ED (ethylenediamine) was prepared as a drug delivery carrier. The structure and molecular weight of polymers were characterized by $^1H$-NMR and gel permeation chromatography. Micelle size and shape were measured by electro-photometer light scattering and transmission electron microscope. The mean diameter of micelles was 23 nm in aqueous solution. To evaluate the potential of these polymeric micelles as a drug carrier, PSI-mPEG-$C_{16}$-ED was conjugated with Cy5.5 for Near-Infrared Fluorescent (NIRF) based optical imaging. PSI-mPEG-$C_{16}$-ED-Cy5.5 was injected intravenously into mice (n=5) and in vivo NIRF imaging was performed during 48 h after injection. The biodistribution study at 24 h after injection showed the longcirculation property of PSI-mPEG-$C_{16}$-ED-Cy5.5. Therefore, PSI-mPEG-$C_{16}$-ED micelles could be a promising drug carrier and imaging agent.

• Journal of Korean Society of Environmental Engineers
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• v.34 no.12
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• pp.855-862
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• 2012

Caenorhabditis elegans, a free-living nematode mainly found in the soil pore water, roles the critical function in trophic levels, energy flow, and decomposition in soil ecosystem. C. elegans is commonly used species to test soil toxicity. Recently, they are employed broadly as a test organism in nanotoxicology. In this study, a review of the toxicity of nanomaterials for C. elegans was presented based on SCI (E) papers. The nanotoxicity studies using C. elegans have been reported in 20 instances including the mechanism of toxicity. Most studies used K-medium, S-medium, and NGM (Nematode Growth Medium) plate as an exposure medium to test toxicity of nanoparticles. The effects observed include anti aging, phototoxicity, genotoxicity, and dermal effects on C. elegans exposed to nanoparticles. We found that the toxic mechanisms were related with various aspects such as lifespan abnormality, oxidative stress, distribution of particles on inter-organisms, and stress-related gene analysis. C. elegans has advantage to test toxicity of nanoparticles due to various cellular activities, full genome information, and easy observation of transparent body. C. elegans was considered to be a good test species to evaluate the nanotoxicity.

• Polymer(Korea)
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• v.30 no.3
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• pp.196-201
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• 2006

Poly (ethylene glycol)-based diblock and triblock thermo- sensitive polyester copolymers were investigated for application on tissue engineering and injectable biomaterials in drug delivery system due to their nontoxicity, biocompatibility and biodegradability. We synthesized the diblock copolymers consisting of methoxy poly (ethylene glycol) (MPEG) (Mn=750 g/mole) and poly $(\varepsilon-caprolactone)$ (PCL) by ring opening polymerization of $\varepsilon-CL$ with MPEG as an initiator in the presence of HCl $Et_2O$. The effect of diblock copolymers on in vivo osteogenic differentiation of rat bone marrow stromal cells (BMSCS) with and without the presence of osteogenic supplements (dexamethasone) was investigated. Thin sections were cut from paraffin embedded tissues and histological sections were stained by H&E, von Kossa, and immunohistochemical staining for osteocalcin. In conclusion, dexamethasone containing thermo- sensitive hydrogel might be improved osteogenic differentiation of BMSCs. We expect the osteoinduction effect to be excellent when it uses stem cell or other osteogenic materials.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.41-41
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• 2018

차꼬리고사리(Asplenium trichomanes L.)는 남방계식물로 제주도에 자생하는 것으로 알려져 있으며, 잎에 광택이 있고 총생하는 식물이다. 주로 실내 외 조경 및 분화소재로 이용되며, 한방에서는 철각봉미초라하여 뿌리를 포함한 전초를 이질, 임병, 만성질염, 월경불순 및 요통의 약재로 사용한다. 또한 식물구계학적 특정식물종 IV급, 적색자료목록 준위협 (NT)에 분류된 식물로 보호가 필요하다. 본 연구는 차꼬리고사리의 대량생산을 위한 기내전엽체 증식 및 기외포자체 형성조건을 구명하고자 수행되었다. 실험재료는 포자를 기내 발아시켜 전엽체를 획득한 다음 8주 간격으로 계대하면서 확보하였다. 전엽체의 증식과 생육에 적합한 배지를 비교하고자, 1/4, 1/2, 1, 2MS와 Knop배지를 조성하여 배양하였다. 배양은 전엽체 300mg을 메스로 균일하게 다지는 방법을 이용하였으며, 배양환경은 온도 $25{\pm}1.0^{\circ}C$, 광도 $30{\pm}1.0{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, 광주기 16/8h(light/dark)로 조절되었다. 실험결과, 1MS배지에 배양된 전엽체의 생체중이 4.3g으로 가장 많이 증가하였다. 형태형성발달도 하트형의 전엽체로 정상적으로 유도되었으며, 생식기관도 관찰되었다. 전엽체로부터 포자체의 형성을 유도하고자, 원예상토, 피트모스, 펄라이트 및 마사토의 비율을 5종류로 달리하여 혼합된 토양을 사각분($7.5{\times}7.5{\times}7.5cm$)에 충진하였다. 전엽체 1g과 증류수 25mL를 핸드블랜더로 10초간 분쇄하여 토양표면에 분주하는 실험방법을 사용하였다. 재배환경은 온도 $25{\pm}1.0^{\circ}C$, 광도 $43{\pm}2.0{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, 광주기 16/8h(light/dark)로 유지하면서 12주간 재배되었다. 실험결과, 원예상토 단용, 원예상토와 펄라이트가 2:1(v:v)로 혼합된 토양, 원예상토와 마사토가 2:1(v:v)로 혼합된 토양에서 각 31.7, 24.3, 19.3개의 포자체가 생산되었다. 한편 포자체의 생육은 원예상토 단용 토양에서 엽수, 엽장, 엽폭 등의 수치가 비교적 우수하였다. 따라서 차꼬리고사리의 전엽체는 MS배지에 배양하고 증식된 전엽체를 원예상토에 분주하여 포자체의 형성을 유도하는 것이 가장 효과적이었다.

• Journal of Bio-Environment Control
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• v.12 no.2
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• pp.83-88
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• 2003

The effect of vertical stratification of perlite by particle size on the growth and yield of tomato In a recycling hydroponics was examined. Vertical stratifications were composed of mixed form of small medium and large size (MP), divided forms of small and large size (smal1/1arge, DP I), and medium and large size (medium/large, DP II). Tomatoes showed higher growth in divided form, specially in DP II than DP I. Deformed fruits had higher occurrence by 17.8% in the mixed form than divided forms, while they were not significantly different between mixed forms. Root activity tended to increase during 20 days to 50 days after transplanting (DAT) in all treatments, but showed highest value at 50 DAT in DP II. After harvest electric conductivity and pH of stratified perlite were slightly higher in upper zone of DP I, but were not different in the other treatments. Mineral contents in the medium after harvest were higher in lower zone than the upper zone in MP and DP II, but vice versa in DP I.

• Journal of Bio-Environment Control
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• v.16 no.4
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• pp.388-394
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• 2007

To product the edible fresh shoots of Sedum sarmentosum in summer season, 4 local strains were cultivated with cutting propagation under 0, 30, 50, 70, 90% shading. Effect of shading on shoot growth, leaf chroma value, SPAD value, and bitterness were investigated at 35 days after cutting. Plant height, the number of node and leaf stem diameter in $30{\sim}90%$ shading were increased than in the control, and Pohang local strain had little-overgrowth shoot, thicker stem, a few brenches per shoot, and larger leaf compared to the other local strains. Fresh and dry weight under shading were increased than in the control, dry weight of pohang local strain was highest in 50% shading. Hunter L and b values were decreased out of proportion to higher shading rate. SPAD value in $50{\sim}90%$ shading was significantly lower than in the control. The bitterness of fresh shoot was decreased out of proportion to higher shading rate. In Summer season, producing the fresh shoot of S. sarmentosum was excellent in 50% shading, and Pohang local strain among 4 local strains was more stable and good in yield and quality under shading.

• Journal of Bio-Environment Control
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• v.19 no.4
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• pp.210-216
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• 2010

This study was carried out to investigate the plant growth of various foliage plants affected by the nutrient solution strength. Ficus benjamina, Hedera helix, Philodendron tatei, Rhapis excelsa, Spathiphyllum spp. were used in this experiment. The Sonneveld solution was diluted to 0, 1/4, 1/2, and 1 folds and applied through a subirrigation system. Plant height and width, leaf number, leaf area, fresh and dry weights of shoots were measured to compare the responses to the different treatments. The required amounts for nutrients were different among the various foliage plants. The growth of Ficus and Philodendron was improved as the nutrient solution strength got higher, but that of Hedera and Spathiphyllum showed the best growth in the lower nutrient solution strength of 1/4 and 1/2 folds. The growth of Rhapis was improved in the nutrient solution strength of 1 fold but the other treatments did not affect on plant growth. N, P, and K were the most important nutrients that had influence on the growth of the foliage plants in this study. There was not an accurate criterion for fertilization and irrigation to each foliage plant. Moreover, the foliage plants grew slowly during the early stage and this period must be shortened for commercial production. Therefore, the experiment was executed to make up these defects. The plants applied with proper strength of Sonneveld solution grew faster and had better quality.

• Journal of Bio-Environment Control
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• v.18 no.3
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• pp.273-279
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• 2009

This study was conducted to identify optimal concentrations of N (nitrogen) and K (Potassium) fertilizers on growth, yield and quality of melon (Cucumis melo. L) when they were grown with a fertigation culture in a greenhouse. Three strength (S) levels of fertilizers, including 1 S, 1/2S, and 1/4S were supplied N and K nutrients as using a trickle irrigation system. When the strength level of fertilizers was increased from 1/4S to 1 S, the level of EC (electronic conductivity) in soil was increased. Soil-water tension was ranged between -15 and -20kPa until fruit setting stage, whereas it was ranged between -45 and -50kPa in the later growth stages. In results, N fertilizer had effects on fruit yield and quality. A higher fruit yield was observed when plants were supplied with 1 S and 1/2S level of N fertilizer. The highest yield of marketable fruit, about 5,086kg/10a, was also observed when plants were supplied with 1/2S N fertilizer. A higher net index and sugar content of fruit was observed in the treatments of 1/2S and 1/4S level of N fertilizer compared to 1 S level. In contrast, there was no statistic difference in the yield and quality with three levels of K fertilizer. Results indicate that the 1/2S level for N and 1/4S level for K fertilizer are effective and optimal for the melon plants grown under the fertigation culture in terms of increasing fruit yield and quality and reducing the cost of fertilizers.

• Korean Journal of Plant Tissue Culture
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• v.27 no.1
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• pp.63-69
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• 2000

In order to establish a micropropagation system for buffalo gourd (Cucurbita foetidissima ) and common milkweed (Asclepias syriaca), the effects of several plant growth regulators and culture temperature on shoot multiplication and rooting were investigated. In buffalo gourd, the greatest number of shoot from shoot tip culture and well growth of formed shoot were obtained on the MIS medium supplemented with 1.0 mg/L BA and 0.3 or 0.6 mg/L IAA. Whereas kinetin and 2iP were not effective for shoot multiplication in vitro. It was found that 22$^{\circ}C$ and $25^{\circ}C$ were suitable for shoot multiplication. Roots were easily formed by the addition of auxins, especially 1.0 or 2.0 mg/L IBA and 2.0 mg/L IAA. Over 90% of plants survived successfully after being transferred into the field. In common milkweed, BA was more effective than kinetin or 2iP for its micropropagation in vitro. The increased shoot weight and number of nodes per shoot were obtained on the medium containing 3.0 mg/L BA and 0.3 or 0.6 mg/L IAA. But 2iP promoted the shoot elongation. In addition. common milkweed was sensitive to culture temperature in vitro. Temperature around 22$^{\circ}C$ was favorable for shoot multiplication and growth, whereas temperature higher than $25^{\circ}C$ usually reduced the rate of shoot survival rate.