DOI QR코드

DOI QR Code

광학 영상을 위한 Cy5.5가 결합된 생체적합성 고분자 마이셀 나노입자의 제조 및 특성분석

Preparation and Characterization of Cy5.5-conjugated Biocompatible Polymeric Micellar Nanoparticles for Optical Imaging

  • 김효정 (한국화학연구원 바이오소재센터,경희대학교 약학대학) ;
  • 김병진 (경희대학교 약학대학) ;
  • 이하영 (한국유나이티드제약) ;
  • 정석현 (한국화학연구원 바이오소재센터) ;
  • 정서영 (한국화학연구원 바이오소재센터) ;
  • 육순홍 (경희대학교 약학대학) ;
  • 신병철 (한국화학연구원 바이오소재센터) ;
  • 성하수 (한국화학연구원 바이오소재센터) ;
  • 최연웅 (한국화학연구원 바이오소재센터) ;
  • 하대철 (한국유나이티드제약) ;
  • 조선행 (한국유나이티드제약) ;
  • 이수민 (한남대학교 화학과)
  • Kim, Hyo-Jeong (Biomaterials Research Center, Korea Research Institute of Chemical Technology,Department of Pharmaceutical Science, College of Pharmacy, Kyung Hee University) ;
  • Kim, Byung-Jin (Department of Pharmaceutical Science, College of Pharmacy, Kyung Hee University) ;
  • Lee, Ha-Yeong (Korea United Pharm) ;
  • Jung, Suk Hyun (Biomaterials Research Center, Korea Research Institute of Chemical Technology) ;
  • Jeong, Seo-Young (Biomaterials Research Center, Korea Research Institute of Chemical Technology) ;
  • Yuk, Soon-Hong (Department of Pharmaceutical Science, College of Pharmacy, Kyung Hee University) ;
  • Shin, Byung-Cheo (Biomaterials Research Center, Korea Research Institute of Chemical Technology) ;
  • Seong, Ha-Soo (Biomaterials Research Center, Korea Research Institute of Chemical Technology) ;
  • Choi, Youn-Woong (Biomaterials Research Center, Korea Research Institute of Chemical Technology) ;
  • Ha, Dae-Chul (Korea United Pharm) ;
  • Choi, Sun-Hang (Korea United Pharm) ;
  • Lee, Soo-Min (College of chemistry, Hannam University)
  • 발행 : 2009.12.20

초록

PHEA (hydroxyethyl-aspartamide)-mPEG (methoy-polyethyleneglycol)-$C_{16}$ (hexadecylamine)-ED (ethylenediamine) was prepared as a drug delivery carrier. The structure and molecular weight of polymers were characterized by $^1H$-NMR and gel permeation chromatography. Micelle size and shape were measured by electro-photometer light scattering and transmission electron microscope. The mean diameter of micelles was 23 nm in aqueous solution. To evaluate the potential of these polymeric micelles as a drug carrier, PSI-mPEG-$C_{16}$-ED was conjugated with Cy5.5 for Near-Infrared Fluorescent (NIRF) based optical imaging. PSI-mPEG-$C_{16}$-ED-Cy5.5 was injected intravenously into mice (n=5) and in vivo NIRF imaging was performed during 48 h after injection. The biodistribution study at 24 h after injection showed the longcirculation property of PSI-mPEG-$C_{16}$-ED-Cy5.5. Therefore, PSI-mPEG-$C_{16}$-ED micelles could be a promising drug carrier and imaging agent.

키워드

참고문헌

  1. V.P. Torchilin, J. Control. Release., 73, 137-172, (2001). https://doi.org/10.1016/S0168-3659(01)00299-1
  2. D.D. Lasic, Nature., 355, 279-280, (1992). https://doi.org/10.1038/355279a0
  3. K. Kazunori, H. Atsushi and N. Yukio, Adv. Drug. Deliver. Rev., 47, 113-131, (2001). https://doi.org/10.1016/S0169-409X(00)00124-1
  4. M. Yokoyama, A. Satoh, Y. Sakurai, T. Okano, Y. Matsumura, T. Kazunori and K. Kataoka, J. Control. Release., 55, 219-229, (1998). https://doi.org/10.1016/S0168-3659(98)00054-6
  5. A. Lavasanifar, J. Samuel and G.S. Kwon, Adv. Drug. Deliver. Rev., 54, 169-190, (2002). https://doi.org/10.1016/S0169-409X(02)00015-7
  6. N. Nishiyama and K. Kataoka, Pharmacology & Therapeutics.,112, 630-648, (2006). https://doi.org/10.1016/j.pharmthera.2006.05.006
  7. M. Yokoyama, S. Fukushima, R. Uehara, K. Okamoto, K. Kataoka, Y. Sakurai and T. Okano, J. Control. Release., 50, 79-92, (1998). https://doi.org/10.1016/S0168-3659(97)00115-6
  8. G. Giammona, G. Puglisi, G. Cavallaro, A. Spadaro and G. Pitarresi, J. Control. Release., 33, 261-271, (1995). https://doi.org/10.1016/0168-3659(94)00091-8
  9. G. Giammona, B. Carlisi and S. Palazzo, J. Polym. Sci. Polym. Chem., 25, 2813-2818, (1987). https://doi.org/10.1002/pola.1987.080251016
  10. G. Cavallaro, M. Licciardi, G. Giammona, P. Caliceti, A. Semenzato and S. Salmaso, J. Control. Release., 89, 285-295, (2003). https://doi.org/10.1016/S0168-3659(03)00121-4
  11. Y.H. Yun and Y.W. Cho, Polym. Sci. and Tech., 18, 4, (2007).
  12. H. Choi, G. Khang, H. Shin, J.M. Rhee and H.B. Lee, Int. J. Pharm., 195, 234-203, (2002).
  13. G.H. Haggis, D. Michie, A.R. Muir, K.B. Roberts and P.M.B. Walker, Longmans (Bristol) Green & Co. LTD., (1965).
  14. M. Veiseh, P. Gabikian, S.B. Bahrami, O. Veiseh, M. Zhang, R.C. Hackman, A.C. Ravanpay, M.R. Stroud, Y. Kusuma, S.J. Hansen, D. Kwok, N.M. Munoz, R.W. Sze, W.M. Grady, N.M. Greenberg, R.G. Ellenbogen and J.M. Olson, Cancer Res., 67, 14, (2007). https://doi.org/10.1158/0008-5472.CAN-06-3948
  15. A. Hansch, I. Hilger, O. Frey, D. Sauner, M. Gajda, M. Haas, A. Malich, J. Bottcher, R. Brauer and W.A. Kaiser, J. Experimental Animal Sci., 43, 129-.139, (2006) https://doi.org/10.1016/j.jeas.2005.04.001
  16. S.R. Mujumdar, R.B. Mujumdar, C.M. Grant and A.S. Waggoner, Biocon. Chem., 7, 356-362, (1996). https://doi.org/10.1021/bc960021b