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http://dx.doi.org/10.4333/KPS.2009.39.6.393

Preparation and Characterization of Cy5.5-conjugated Biocompatible Polymeric Micellar Nanoparticles for Optical Imaging  

Kim, Hyo-Jeong (Biomaterials Research Center, Korea Research Institute of Chemical Technology,Department of Pharmaceutical Science, College of Pharmacy, Kyung Hee University)
Kim, Byung-Jin (Department of Pharmaceutical Science, College of Pharmacy, Kyung Hee University)
Lee, Ha-Yeong (Korea United Pharm)
Jung, Suk Hyun (Biomaterials Research Center, Korea Research Institute of Chemical Technology)
Jeong, Seo-Young (Biomaterials Research Center, Korea Research Institute of Chemical Technology)
Yuk, Soon-Hong (Department of Pharmaceutical Science, College of Pharmacy, Kyung Hee University)
Shin, Byung-Cheo (Biomaterials Research Center, Korea Research Institute of Chemical Technology)
Seong, Ha-Soo (Biomaterials Research Center, Korea Research Institute of Chemical Technology)
Choi, Youn-Woong (Biomaterials Research Center, Korea Research Institute of Chemical Technology)
Ha, Dae-Chul (Korea United Pharm)
Choi, Sun-Hang (Korea United Pharm)
Lee, Soo-Min (College of chemistry, Hannam University)
Publication Information
Journal of Pharmaceutical Investigation / v.39, no.6, 2009 , pp. 393-400 More about this Journal
Abstract
PHEA (hydroxyethyl-aspartamide)-mPEG (methoy-polyethyleneglycol)-$C_{16}$ (hexadecylamine)-ED (ethylenediamine) was prepared as a drug delivery carrier. The structure and molecular weight of polymers were characterized by $^1H$-NMR and gel permeation chromatography. Micelle size and shape were measured by electro-photometer light scattering and transmission electron microscope. The mean diameter of micelles was 23 nm in aqueous solution. To evaluate the potential of these polymeric micelles as a drug carrier, PSI-mPEG-$C_{16}$-ED was conjugated with Cy5.5 for Near-Infrared Fluorescent (NIRF) based optical imaging. PSI-mPEG-$C_{16}$-ED-Cy5.5 was injected intravenously into mice (n=5) and in vivo NIRF imaging was performed during 48 h after injection. The biodistribution study at 24 h after injection showed the longcirculation property of PSI-mPEG-$C_{16}$-ED-Cy5.5. Therefore, PSI-mPEG-$C_{16}$-ED micelles could be a promising drug carrier and imaging agent.
Keywords
PHEA (hydroxyethyl-aspartamide); micelle; Cy5.5 NHS-ester; NIRF (Near-Infrared Fluorescent); optical imaging;
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