• Title/Summary/Keyword: 생육형질

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Effects of Burning Treatment on Development, Seed Yield, and Reduction of Thatch in Zoysia-82 (입화가 Zoysia-82의 생육, 종자수량 및 Thatch 감소에 미치는 효과)

  • 김인섭;김달웅
    • Asian Journal of Turfgrass Science
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    • v.3 no.1
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    • pp.16-23
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    • 1989

  • 입화(Burning)가 zoysia-82의 생육, 종자생산 및 thatch 감소에 미치는 효과를 조사한 결과는 다음과 같다. 1. 유초출현일수는 대비구(자연상태)와 유초출현 약 2개월전에 입화처리한 구가 짧았다. 2. 종자수량은 대비구가 가장 많았고 휴면직전과 유초출현직후의 입화구가 가장 적었다. 3. Thatch 축적은 대비구가 가장 깊었으며 휴면직전과 휴면 약 2개월 후 입화처리구가 가장 얇았다. 4. 근건중, runner 건중, 총 건물중은 휴면 약 2개월후 입화구가 가장 무거웠으며, 엽 및 엽경건중은 휴면직후의 입화구가 가장 무거웠으나 근건중 이외의 형질들은 입화처리간 유의차가 없었다. 5. Zoysia-82의 유초출현일수의 단축과 종자수량의 증가및 thatch 감소를 위해서는 유초출현 약 2개월전과 휴면 약 2개월후가 입화적기라 사료된다.

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Development of a Recombinant Strain of Pichia pastoris with Antibacterial Activity (항균활성을 보유한 재조합 Pichia pastoris 균주의 개발)

  • 강대욱;이준원;허건영;안종석
    • Journal of Life Science
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    • v.12 no.4
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    • pp.496-503
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    • 2002

  • To develop a yeast strain of Pichia pastoris producing an antibacterial peptide, we have attempted the expression and secretion of an insect defensin. The nucleotide sequences corresponding to mature defensin were chemically synthesized by 6 oligomers, assembled in vitro and the synthesized gene was identified by nucleotide sequencing. The prepro sequence of yeast mating factor $\alpha$1 and the defensin gene were recombined into a Pichia expression vector, pPIC9K. The resulting plasmid, pPIDE, was transformed into P. pastoris GSl15 and transformants selected on histidine-deficient minimal plates were tested for antibacterial activity against Micrococcus luteus. Four strains with different antibacterial activity were selected for further analysis. Southern hybridization and RT-PCR verified the defensin gene was maintained and transcribed in a host. Four strains were cultivated in YPD broth for 96 hours to compare cell growth and antibacterial activity, They showed no difference in cell growth, however, each strain showed different antibacterial activity pattern with culture time. The maximal activity was about 550 AU/ $m\ell$.

  • https://doi.org/10.5352/JLS.2002.12.4.496 인용 PDF KSCI