• Journal of Food Hygiene and Safety
• /
• v.35 no.6
• /
• pp.567-573
• /
• 2020

The persimmon is commonly cultivated in temperate regions of the world, including China, Korea, Japan, Brazil, Turkey, and Italy. In some Asian cultures, consumers are aware of the health claims related to the persimmon and its functional ingredients. The rich phytochemistry of the persimmon has opened new avenues of research on diet-based regimens designed to cure various ailments. This study was conducted to identify the genotoxicity of immature green persimmon (Diospyros kaki THUNB.) extract (DKA). The bacterial reverse mutation assay, the chromosomal aberration assay, and the mammalian micronucleus test were performed to determine the DKA genotoxicity. The result of the bacterial reverse mutation assay revealed that the DKA did not induce mutagenicity in Salmonella typhimurium TA98, TA100, TA1535, TA1537 and Escherichia coli WP2uvrA with or without metabolic activation of S9 mixture. The oral administration of DKA also caused no significant increase in the number of micronucleated polychromatic erythrocytes or in the mean ratio of polychromatic to total erythrocytes. In addition, DKA did not cause a significant chromosome aberration on CHL cells in the presence or absence of S9 activation. In conclusion, DKA could be considered as a reliable and safe functional food since no toxicity was found under the condition of this study.

• 한국유가공학회:학술대회논문집
• /
• 2002.11a
• /
• pp.57-65
• /
• 2002

• Bioindustry News
• /
• v.13 no.3
• /
• pp.31-38
• /
• 2000

• Proceedings of the Korean Information Science Society Conference
• /
• 2004.04b
• /
• pp.277-279
• /
• 2004

생물을 진화적으로 분석할 때, 보전적인 유전자(Conserved gene)득은 기능이 알려지지 못했던 다양한 생물학적 정보를 얻어내는데 유용하게 쓰일 수 있다. 특히 완전히 서열이 밝혀진 지놈(Genome) 데이터로부터 진화적으로 보존적인 유전자 서열의 상동성에 따른 분류를 통한 2차 데이터베이스의 구축은 생물학자들에게 다차원적인 정보를 제공 할 수 있다. 이미 이러한 데이터베이스가 다양한 방법에 따라 구축되었고 생물학자들의 연구에 활용되고 있다. 그러나 기 구축된 데이터베이스들은 생물학자들이 이용하기에 Paralogs의 포함 문제점으로 인해 신뢰성이 떨어지거나 데이터베이스 생성기간이 오래 걸린다는 단전이 있다 본 연구는 기존에 구축된 데이터베이스들의 구축방법을 응용하고, 정보기술을 활용하여 빠르고 효과적으로 정확성을 높인 새로운 구축 방법론과 데이터베이스를 활용한 분석 시스템에 대해 제시하고자 한다.

• Proceedings of the Korean Society for Agricultural Machinery Conference
• /
• 2003.02a
• /
• pp.209-214
• /
• 2003

바이오 센서는 생물학적 인식 반응(Biological recognition reaction)이나 생물 촉매 공정(Bio-catalytic process)을 측정 가능한 전기적 신호로 변환시켜주는 적절한 변환기(Transducer)와 같은 생물학적 인식요소로 이루어진 감지 장치를 의미한다. 1962년 효소를 이용한 Glucose 전극봉의 개발이후 이 분야에 대한 수많은 연구와 기기의 개발이 진행되어왔다. 분석도구로써 바이오 센서는 효소, 항체, 수용체, 세포, 조직 등과 같은 생물학적 인식 요소들에 광범위하게 적용 가능하다는 장점이 있는 반면, 위에 열거한 생물학적 인식 요소들의 불안정성으로 인해 신뢰성이 유지되지 못하는 단점을 동시에 지니고 있다. (중략)

• Journal of Korean Society of Forest Science
• /
• v.108 no.1
• /
• pp.77-87
• /
• 2019

While the expansion of protected areas in accordance with Korea's National Biodiversity Strategy contributions to the conservation of biological diversity, it incurs economic losses and poses threats to the livelihoods of local residents. Payment for ecosystem services (PES) compensates local residents for their economic damages in a sustainable manner. To assess feasibility of PES, there is a need to estimate the general public's benefits from biodiversity conservation in protected areas. This study attempted to estimate visitors' willingness to pay (WTP) for biodiversity conservation in Mt. Jiri using the contingent valuation method (CVM). The WTP of respondents for the expansion of protected areas to increase biodiversity conservation was estimated at 16,103 KRW per household per year and this equates to a total of 316.8 billion KRW for all the households in Korea. The higher a respondent's age, education level and household income level, the more willing he or she is to pay for biodiversity conservation. Likewise, respondents who are aware of Mt. Jiri's designation as an Ecosystem and Landscape Conservation Area are more willing to pay for biodiversity conservation. Moreover, the higher the importance placed on biodiversity conservation policy, the higher the willingness to pay for biodiversity conservation. To enhance the feasibility of PES schemes, it is necessary to improve the general public's knowledge and awareness of biodiversity by providing opportunities for education and more information on biodiversity and biodiversity policies.

• Journal of Korean Society of Environmental Engineers
• /
• v.29 no.9
• /
• pp.997-1002
• /
• 2007

The objectives of this research are to remove dissolved organic matter and nitrogen compounds by using aerated submerged bio-film(ASBF) reactors in batch systems and improve understanding of dissolved organic matter and nitrogen compounds removal rates with dynamic relationships between heterotrophic and autotrophic bacteria in the fixed-film reactor. This research explores the possibility of enhancing the performance of shallow wastewater treatment lagoons through the addition of specially designed structures. These structures are designed to encourage the growth of a nitrifying bacterial bio-film on a submerged surface. Specially, the effects of cold temperatures on the dissolved organic matter and ammonia nitrogen performance of the ASBF pilot plant was investigated for the batch system. It is anticipated thai the ASBF would be used for a design of biological treatment for removing of dissolved organic matter and nitrogen compounds in new wastewater treatment plants as well as existing wastewater treatment plants.

• Journal of Korean Society of Environmental Engineers
• /
• v.22 no.10
• /
• pp.1893-1904
• /
• 2000

Nitrogen compounds are one of the major pollutants which cause eutrophication problems of the river or lake and red tides problems of the ocean. Currently available technologies for the removal of nitrogen compounds are mostly biological treatment. However, biological treatment is only effective for the wastewater which contains low concentration of nitrogen compounds. Leachate from solid waste landfill or industrial wastewater which contains high concentration of nitrogen can not be effectively treated by most of the currently available biological treatment technologies. With this connection. the objective of this study is to examine the applicability of ammonia stripping technology for the removal of high concentration of ammonia nitrogen compounds of the leachate from solid waste landfill. It can be concluded that ammonia stripping technology which was placed before the biological treatment process was very effective for the removal of high concentration of ammonium compounds. The chemical cost for the ammonia stripping was 16 percent higher than MLE process, so other methods like sludge recycling are needed for the reduction of operation cost. Further details are discussed in this paper.

• 한국신재생에너지학회:학술대회논문집
• /
• 2007.06a
• /
• pp.763-766
• /
• 2007

국내에서 발생되는 고형폐기물 중 자원으로 재활용 가능한 유기성폐기물은 하수슬러지, 음식물류폐기물, 축산분뇨 등을 대표 할 수 있다. 이들 유기성폐기물은 환경적인 측면에서 볼 때 다른 유기성 폐수 및 폐기물에 비하여 오염부하량이 상대적으로 높지만, 이를 생물자원 (Biomass)으로 인식하고 이용 할 경우 지구온난화와 같은 환경문제 뿐만아니라 향후 자원고갈문제를 동시에 해결할 수 있는 대체에너지 자원이다. 유기성폐기물을 대체에너지 자원으로 효율적으로 이용하기 위해서는 우선적으로 국제적 환경규제와 에너지 정책에 능동적으로 대응할 수 있는 자원순환형 폐기물관리 시스템 구축이 필요하며, 이를 위한 체계적인 정책적 지원책과 기술 개발이 뒷받침 되어야 할 것이다. 자원 재활용과 에너지회수 기술에 있어 혐기성소화(anaerobic digestion)는 유기성폐기물의 효과적인 감량화, 재이용화, 안정화를 만족시키는 동시에 유용 에너지원인 메탄가스를 회수할 수 있는 바이오가스 전환기술로 최근에 주목을 받는 biotechnology 중의 하나로 자리매김 하고 있다. 특히, 소비자원의 대부분이 해외에 의존하는 국내현실과 최근 고유가에 따른 국가 에너지 정책을 제고해야하는 현 시점에서 유기성폐기물을 이용한 바이오가스화 기술을 널리 보급하기 위해서는 요소기술 개발과 정부의 적극적인 정책적 지원 방안이 마련되어야 할 것이다.

• Journal of Life Science
• /
• v.31 no.8
• /
• pp.755-760
• /
• 2021

Swine diarrhea is a livestock disease that causes huge economic losses to pig farms. In general, diarrhea occurs because of the proliferation of pathogenic Escherichia coli (E. coli). The toxins produced by the proliferated E. coli cause edema in pigs. Although the proliferation of these coliforms can be prevented by using a vaccine, the vaccines containing chemically produced dead bacteria are not very effective, making it difficult to control the proliferation of E. coli. Therefore, there is a need to develop new, more effective vaccines. In this study, we prepared killed F4+ and F18ab+ E. coli, which induce diarrhea and edema in pigs, using the extracts of immune-induced silkworms containing antimicrobial peptides and examined their availability as a killed-bacteria vaccine. First, the antimicrobial activity analysis of the prepared immune-induced silkworm extract was conducted using the radial diffusion assay. The results showed high activity against both F4+ and F18ab+ E. coli. The production efficiency of E. coli dead cells was determined using the colony-counting method. The concentration of the E. coli dead cells was the highest (50 mg/ml) when treated at 4℃. In addition, the analysis of the prepared dead cells using a transmission electron microscope confirmed that E. coli leaked out of the cytoplasm and the cell membrane remained intact. Therefore, F4+ and F18ab+ E. coli produced using immune-induced silkworms extract are considered to be highly available as bacterial ghost vaccines that can help prevent swine diarrhea and the resulting edema.