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http://dx.doi.org/10.5352/JLS.2021.31.8.755

Inactivation of Pathogenic Escherichia coli Using Crude Extract of Immunized Silkworm  

Park, Jong Woo (Department of Agricultural Biology, National Institute of Agricultural Science, RDA)
Jeong, Chan Young (Department of Agricultural Biology, National Institute of Agricultural Science, RDA)
Lee, Chang Hoon (Department of Agricultural Biology, National Institute of Agricultural Science, RDA)
Kang, Sang Kuk (Department of Agricultural Biology, National Institute of Agricultural Science, RDA)
Ju, Wan-Taek (Department of Agricultural Biology, National Institute of Agricultural Science, RDA)
Kim, Seong-Wan (Department of Agricultural Biology, National Institute of Agricultural Science, RDA)
Kim, Nam-Suk (Department of Agricultural Biology, National Institute of Agricultural Science, RDA)
Kim, Kee Young (Department of Agricultural Biology, National Institute of Agricultural Science, RDA)
Publication Information
Journal of Life Science / v.31, no.8, 2021 , pp. 755-760 More about this Journal
Abstract
Swine diarrhea is a livestock disease that causes huge economic losses to pig farms. In general, diarrhea occurs because of the proliferation of pathogenic Escherichia coli (E. coli). The toxins produced by the proliferated E. coli cause edema in pigs. Although the proliferation of these coliforms can be prevented by using a vaccine, the vaccines containing chemically produced dead bacteria are not very effective, making it difficult to control the proliferation of E. coli. Therefore, there is a need to develop new, more effective vaccines. In this study, we prepared killed F4+ and F18ab+ E. coli, which induce diarrhea and edema in pigs, using the extracts of immune-induced silkworms containing antimicrobial peptides and examined their availability as a killed-bacteria vaccine. First, the antimicrobial activity analysis of the prepared immune-induced silkworm extract was conducted using the radial diffusion assay. The results showed high activity against both F4+ and F18ab+ E. coli. The production efficiency of E. coli dead cells was determined using the colony-counting method. The concentration of the E. coli dead cells was the highest (50 mg/ml) when treated at 4℃. In addition, the analysis of the prepared dead cells using a transmission electron microscope confirmed that E. coli leaked out of the cytoplasm and the cell membrane remained intact. Therefore, F4+ and F18ab+ E. coli produced using immune-induced silkworms extract are considered to be highly available as bacterial ghost vaccines that can help prevent swine diarrhea and the resulting edema.
Keywords
Antimicrobial peptides; killed bacteria; silkworm; swine edema;
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