• Title/Summary/Keyword: 생물농약

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Comparison of the Susceptibility of Freshwater Organisms for the Acute Toxicity Test of Pesticides. (농약의 급성독성 평가를 위한 담수생물의 감수성 비교연구)

  • Shin, Chun-Chul;Lee, Sung-Kyu;Roh, Jung-Koo
    • Korean Journal of Environmental Agriculture
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    • v.5 no.2
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    • pp.130-134
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    • 1986
  • This study was performed to determine the aquatic toxicity of 3 chemicals(butachlor, trichlorfon, and BPMC) to 3 teat organisms (fish, crustacean, and algae) which represent each trophic level in freshwater ecosystem, and to compare the sensitivity of these organisms to 3 chemicals with short-term test. Scenedesmus subspicatus, unicellular algae, was the most sensitive species of 3 organisms to butachlor and the ratio between least and most sensitive organisms was 5.7. Moina rectirostris, freshwater invertebrate, was also more sensitive organism than S. subspicatus and Oryzias latipes to trichlorfon and BPMC, and their ratios were 260,000 and 5,090. As a result, remarkable differences were observed in the sensitivities among the test organisms with different chemical structure and mode of action of the chemicals. Therefore, it is recommended that a set of tests on different species, including the representative species of fish, invertebrate, and algae, should be required in short-term aquatic toxicity test for chemicals introduced into the Korean environment.

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Evaluation of Toxicity of Pesticides against Honeybee (Apis mellitera) and Bumblebee (Bombus terrestris) (꿀벌과 서양뒤영벌에 대한 농약의 독성평가)

  • Ahn, Ki-Su;Oh, Mann-Gyun;Ahn, Hee-Geun;Yoon, Chang-Mann;Kim, Gil-Hah
    • The Korean Journal of Pesticide Science
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    • v.12 no.4
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    • pp.382-390
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    • 2008
  • This study was performed to evaluate the acute toxicity and residual toxicity of the 69 kinds of agrochemicals (41 insecticides, 18 fungicides, and 10 acaricides) against honeybee, Apis mellifera and bumblebee, Bombus terrestris. According to the IOBC standard, the toxicity showed below 30% was classified as non-toxic. Among 41 insecticides, five insecticides (acetamiprid, chlorfenapyr, thiacloprid, milbemectin, and buprofezin+amitraz) against the honeybee; eight insecticides (methomyl, thiodicarb, acetamiprid, chlorfenapyr, thiacloprid, abamectin, spino sad, buprofezin+amitraz) against the bumblebee did not show any toxic effect. Therefore, it thought to being safe. Other 18 fungicides and 10 acaricides were safe against the honeybee and bumblebee. In residual toxicity against the honeybee, eight insecticides (dichlorvos, methomyl, imidachlorprid, emamectin benzoate, spinosad, cartap hydrochloride, chlorfenapyr, and endosulfan) among 41 insecticides tested were safe at three days after treatment; however, sixteen insecticides (dimethoate, fenitrothion, fenthion, methidathion, phenthoate, pyraclofos, fenpropathrin, clothianidin, dinotefuran, thiamethoxam, abamectin, acetamiprid+ethofenprox, acetamiprid+indoxacarb, bifenthrin+imidacloprid, ethofenprox+phenthoate, imidacloprid+methiocarb) still remain high toxicity at eleven days after treatment. Against the bumblebee, residual toxicity showed as safe in seven insecticides (dimethoate, methidation, a-cypermethion, ethofenprox, indoxcarb, chlorpyrifos+a-cypennethrin, esfenvalerate+fenitrochion) at three days after treatment; however, eight insecticides (fenitrothion, pyraclofos, clothianidin, fipronil, acetamiprid+ethofenprox, chlorpyrifos+bifenthrin, ethofenprox+phenthoate, imidacloprid+methiocarb) still showed high toxicity at seven days after treatment. From above results, it will be useful information to select insecticides being safe and effective against the honeybee and bumblebee.

Comparative Evaluation of the Analytical Methods used to Determine Pesticide Residues in Milk via Dispersive Solid Phase Extraction (Dispersive Solid Phase Extraction을 사용한 우유 내 잔류농약 다성분 동시 분석법 비교연구)

  • Oh, Nam Su;Shin, Yong Kook;Lee, Ji Young;Baick, Seung Chun
    • Journal of Dairy Science and Biotechnology
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    • v.33 no.1
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    • pp.27-34
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    • 2015
  • The aim of this study was to optimize a simple, fast, and economic analytical method for the simultaneous determination of various pesticides (aldrin, p,p'-DDT, o,p'-DDT, p,p'-DDE, α-endosulfan, β-endosulfan, dieldrin, heptachlor, permethrin, chlordane, deltamethrin, diazinon, bifenthrin, methoprene, propargite, fenpropathrin, cypermethrin, fenvalerate, and fenpropathrin) in milk by using dispersive solid phase extraction (SPE). In this study, two different extraction methods (low temperature cleanup and liquid-liquid partitioning), which were followed by a cleanup process based on dispersive-SPE, were evaluated and compared for the 19 pesticides. The results for all the pesticides were determined by gas chromatography mass spectrometry (GC/MS) with selected-ion monitoring mode, and the matrix effect of the method was evaluated. Comparison of these approaches yielded higher and more consistent recoveries of most pesticides at fortification levels of 1μg/mL using low-temperature fat precipitation, followed by cleanup process based on dispersive-SPE with PSA and C18 as sorbents, than other preparation process. The relative standard deviation was <20 % and the combination of this method were very effective for the cleanup.

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Evaluation and Comparison with Standard 48 hr Acute Bioassay and High Temperature Rapid Toxicity Test for Sewage Toxicity Test (하수의 독성평가를 위한 표준독성시험법과 온도증가 단기독성평가법의 비교 평가)

  • Lee, Sang-Ill;Jun, Byong-Hee;Weon, Seung-Yeon;Kim, Yi-Jung;Kim, Keum-Yong
    • Journal of Korean Society of Environmental Engineers
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    • v.27 no.2
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    • pp.191-197
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    • 2005
  • A new method, ToxTemp (TOXcity test based on TEMPerature control) using Ceridaphnia dubia was applied to evaluate the toxicity of insecticide materials and compared with the standard 48 hr acute bioassay. BPMC, diazinon and fenitrothion may cause the inhibition to the biological process in sewage treatment plant and need to detect toxicity within short contact time. The ToxTemp method showed sensitive detection with more shorter contact of 1-1.5 hr time than that of the standard 48 hr acute bioassay. To evaluate toxicity of real wastewater/sewage, the inhibition rate of nitrification and oxygen uptake rate (OUR) using activated sludge, the standard 48hr acute bioassay and ToxTemp method using C. dubia were compared, respectively. On the basis of the inhibition rate of nitrification, the OUR test showed the less sensitive results at the relatively strong toxic sewage. On the other hands, the standard 48hr acute bioassay and ToxTemp method using C. dubia represented the toxicity of each wastewater/sewage with high sensitivity. Even the slightly low (about 1.5%) sensitivity, the ToxTemp method showed the high applicability to the real site of sewage treatment plant.

Effects on EDC-like farming chemicals in aquatic Organism (환경생물에 대한 내분비교란물질 의심 농약의 영향)

  • Kim, Hyun-Woo;Park, Kun-Ho;Park, Jin-Hong;Jin, Hua;Kim, Joon-Seong;Eu, Gook-Jong;Cho, Hyun-Sun;Kang, Ga-Mi;Lee, Myung-Sung;Song, Byung-Hoon;Shin, Jin-Sup;Cho, Maing-Haing
    • The Korean Journal of Pesticide Science
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    • v.7 no.3
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    • pp.188-197
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    • 2003
  • Endocrine disrupting chemicals (EDCs) can alter hormone regulation that control reproductive system in animals. The effects of endosulfan, molinate, and alachlor that suspected to have examined disruption EDCs effect on a fish species of interest, Xiphophorus helleri (swordtail fish), were studied using vitellogenin (Vtg) and aromatase as diagnostic biomarkers. Induction of Vtg proteins was detected by RT-PCR in male fish treated with alachlor, and mixture of endosulfan and molinate in dose response manner. Also, induction of aromatase was detected by RT-PCR in male fish treated with alachlor, endosulfan, and mixture of endosulfan and molinate in sinlilar manner. In this study, swordtail fish exposed to endosulfan or molinate individually did not show any adverse effects. However, Vtg and aromatase expressions and apoptosis were detected in swordtail. fish exposed to the mixture of endosulfan and molinate. These results suggested that low concentrations of mixture of molinate and endosulfan individually do not affect swordtail fish, but may influence genital system, and induce apoptosis.

The Detection and a Quantitative Evaluation of Entomopathogenic Nematodes in Cultivated Rhizosphere Soil (경작지 근권 토양내 곤충병원성 선충의 검출 및 정량적 평가)

  • 황경숙;한상미;김윤지;남필원;한송이
    • Korean Journal of Environmental Biology
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    • v.21 no.3
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    • pp.271-275
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    • 2003
  • The direct count and MPN (Most Probable Number) methods were used to measure the number of nematodes in soils collected from cultivated and non-cultivated fields. As a result, the number of nematodes from cultivated soils was higher than the non-cultivated soils (NC -1, NC -2). On the other hand, upon measuring the value from the organo farming cultivated soils (OC, OR) and conventional cultivated soils (CC, CR), the former showed 16 times higher than the latter. These results indicate that nematode population which can multiply in the organic compounds abundantly exist in the organo farming cultivated soil. Isolated entomopathogenie nematodes are composed of two orders, which were Rhabditida and Diplogasterida. To determine the pathogene-city of them using the 5th larvae and pupae silkworm, and the following mean LD90 values were found: 24 to 30 hours in Rhabditida and 36 to 48 hours in Diplogasterida nematode, respectively. This study indicates that nematodes are sensitive to this kind of environmental disturbance. Isolated entomopathogenic nematodes were suggested that aye quite within the realms of possibility for biological control agents.

Biological Control of Garlic White Rot Accused by Sclereotium cepivorum and Sclereotium sp. Using Bacillus subtilis 122 and Trichoderma harzianum 23 (Bacillus subtilis 122와 Trichoderma harzianum 23에 의한 마늘 흑색썩음균핵병의 생물적 방제)

  • Lee Sang-Yeob;Lee Sang-Bum;Kim Yong-Ki;Hwang Soon-Jin
    • Research in Plant Disease
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    • v.12 no.2
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    • pp.81-84
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    • 2006
  • Trichoderma harzianum 23 WP and Bacillus subtilis 122 WP were formulated as antagonists of Sclerotium cepivorum and Sclerotium sp. of garlic white rot. In the field test, applications of Trichoderma harzianum WP and Bacillus subtilis WP reduced garlic white rot by Sclerotium cepivrum from 10.9% in the control to 4.1% and 6.2%, respectively at Taean. Also at Seosan, applications of Trichoderma harzianum 23 WP and Bacillus subtilis 122 WP reduced garlic white rot by Sclerotium sp. from 17.8% in the control to 1.2% and 2.6%, respectively. Treatment of Trichoderma harzianum 23 WP and Bacillus subtilis 122 WP increased garlic yield in two area. Therefore, Trichoderma harzianum 23 WP and Bacillus subtilis 122 WP have shown potential as biofungicides of garlic white rot in the two different pathogens.

Isolation and Identification of ura5 Gene in Entomopathogenic Fungus, Metarhizium anisopoliae (살충성곰팡이 Metarhizium anisopliae의 ura5 유전자의 분리동정)

  • Park, In-Cheol;Lee, Dong-Kyu;Kang, Sun-Cheol;Hwang, Cher-Won
    • Applied Biological Chemistry
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    • v.40 no.1
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    • pp.30-33
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    • 1997
  • About 250 bp ura5 gene (Orotate phosphoribosyl transferase) fragment was cloned from genomic DNA of entomopathogenic fungus Metarhizium anisopliae by using PCR method. Entire nucleotide sequences of cloned DNA fragment were determined and analysed as compared with other fungus ura5 genes. The amino acid sequence deduced from the nucleotide sequence showed 85.5% homology to ura5 protein of Trichoderma reesei. Using this 250 bp PCR fragment we have isolated full ura5 gene of M. anisopliae by genomic Southern hybridization and the isolated 4.4 kb DNA fragments were mapped by restrictional enzyme.

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Isolation of Polyene Antifungal Antibiotics Against Gummy Stem Light Caused by Didymella bryoniae (Streptomyces sp. 유래 Polyene 계 항만고병 항생물질의 분리)

  • 김광석;서영배
    • Microbiology and Biotechnology Letters
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    • v.32 no.3
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    • pp.238-242
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    • 2004
  • Antifungal agents, flavofungin and fungichromin were isolated from the fermentation culture broth of a Streptomyces sp. SKM338. Biological evaluation of these antibiotics indicated that the compounds possesses broad spectrum antifungal activity against various pathogens. Especially, these compounds inhibited throughly growth of Didymella bryoniae, caused Gummy stem blight of melons, occurs in the southeastern Korea. Inhibition of this pathogen may be prevented from directly reducing both pre- and post-harvest yields.

Epoxide Hydrolase-catalyzed Hydrolytic Kinetic Resolution for the Production of Chiral Epoxides (에폭사이드 가수분해효소에 의한 동력학적 가수분해반응을 이용한 광학활성 에폭사이드 생산)

  • 이은열
    • KSBB Journal
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    • v.17 no.4
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    • pp.321-325
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    • 2002
  • Chiral epoxides are valuable intermediates for the asymmetric synthesis of enantiopure bioactive compounds. Microbial epoxide hydrolases (EHs) are newly discovered enzymes and versatile biocatalysts for the preparation of chiral epoxides by enantioselective hydrolysis of cheap and easily available racemic epoxide substrates. EHs are commercially potential biocatalysts due to their characteristics such as high enantioselectivity, cofactor-independent catalysis, and easy-to-Prepare catalysts. In this Paper, recent progresses in biochemistry and molecular biology of EH and developments of novel reaction systems are reviewed to evaluate the commercial feasibility of EH-catalyzed hydrolytic kinetic resolution for the production of chiral epoxides.