Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation of Polyene Antifungal Antibiotics Against Gummy Stem Light Caused by Didymella bryoniae

Streptomyces sp. 유래 Polyene 계 항만고병 항생물질의 분리

  • Published : 2004.09.01
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Abstract

Antifungal agents, flavofungin and fungichromin were isolated from the fermentation culture broth of a Streptomyces sp. SKM338. Biological evaluation of these antibiotics indicated that the compounds possesses broad spectrum antifungal activity against various pathogens. Especially, these compounds inhibited throughly growth of Didymella bryoniae, caused Gummy stem blight of melons, occurs in the southeastern Korea. Inhibition of this pathogen may be prevented from directly reducing both pre- and post-harvest yields.

D. bryoniae를 원인균으로 하는 참외 만고병에 대해서 항만고병 활성물질을 생산하는 미생물을 방선균으로부터 선별 분리한 후 배양액으로부터 항균활성물질의 규명을 시도하였다. 항진균 물질과 같은 이차대사산물의 생산에 증가시키는 $K_2$$HPO_4$와 칼슘이온이 포함된 GSS배지에서 방선균 SKM338 균주를 180 rpm, $30^{\circ}C$, 5일 동안 배양하여 얻어진 배양 상등액으로부터 물리화학적인 방법으로 항진균 활성이 있는 물질을 분리 정제한 결과 참외의 만고병에 대한 생물농약으로 개발 가능한 방선균 유래의 항진균성 물질은 NMR, IR, UV 및 Mass spectral data 분석 등을 통해 polyene macrolide계에 속하는 항생물질인 Flavofungin, Fungichromin, Filipins로 밝혀졌으며 이들의 응용을 기대해 본다.

Keywords

References

  1. Biochemistry v.7 no.2 The filipin complex Bergy, M. E.;T. E. Eble https://doi.org/10.1021/bi00842a021
  2. Tetrahedron Lett. v.7 The structure of flavofungin Bognar, R.;B. O. Brown;W. J. S. Locklry;S. Makleit;T. P. Toube;B. C. L. Weedon;K. Zsupan
  3. J. Am. Chem. Soc. v.77 Filipin, an antifungal antifungal antibiotic: Isolation and properties Whitfield, G. B.;T. D. Brock;A. Ammann;D. Gottlied;H. E. Carter https://doi.org/10.1021/ja01623a032
  4. J. Ferment. Technol. v.65 Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes Masayuki, H.;H. Nonomura https://doi.org/10.1016/0385-6380(87)90108-7
  5. J. Ferment. Technol. v.65 Efficacy of artificial humic acid as a selective nutrient in HV agar used for the isolation of soil actinomycetes Masayuki, H.;H. Nonomura https://doi.org/10.1016/0385-6380(87)90001-X
  6. J. Chem. Tech. Biotechnol. v.31 Antibiotics from the newly isolated Streptomyces elizabethii. I. Production of a polyene antifungal agent (Elzabethin) and actinomycin in shake flask ucltures John, P. S.;S. M. A. Pickett;A. M. Pickett;E. J. Vandamme;C. W. Bird https://doi.org/10.1002/jctb.280310123
  7. Plant Disease v.79 Fungicide timing for optimum management of gummy stem blight epidemics on watermelon Keinath, A. P. https://doi.org/10.1094/PD-79-0354
  8. Plant Disease v.80 Soil amendment with cabbage residue and rotation to reduce gummy stem blight and increase growth and yield of watermelon Keinath, A. P. https://doi.org/10.1094/PD-80-0564
  9. Plant Disease v.82 Resistance to bonomyl and thiophanatemethyl in Didymella bryoniae from South California and New York Keinath, A. P. https://doi.org/10.1094/PDIS.1998.82.5.479
  10. Phytopathology v.85 Morphological, pathological, and genetic differentiation of Didymella bryoniae and Phoma spp. isolated from cucurbits Keinath, A. P.;M. W. Farnham;T. A. Zitter https://doi.org/10.1094/Phyto-85-364
  11. HortScience v.28 Resistance to gummy stem blight in muskmelon McGrath, D. J.;Vawdrey, L.;Wlker, I. O.
  12. J. Am. Chem. Soc. v.110 Biosynthesis and full NMR assignment of fungichromin, a polyene antibiotic from Streptomyces cellulosae Hiroshi, N.;P. H. Harrison;K. Arai;T. T. Nakashima, L. A. Trimble;J. C. Vederas https://doi.org/10.1021/ja00217a041
  13. Br. J. Pharmacol. Sep. v.134 Inhibitory effects of brefeldin A, a membrane transport blocker, on the bradykinin-induced hyperpolarizationmediated relaxation in the porcine coronary artery Ohnishi Y;K. Hirano;J. Nishimura;M. Furue;H. Kanaide https://doi.org/10.1038/sj.bjp.0704246
  14. Infection v.25 In vivo and in vitro antifungal activity of the polyene derivative SPA-S-753 against encapsulated from of Cryptococcus neoformans Strippoli V.;F. D. D'Auria;N. Simonetti;D. Basti;T. Bruzzese https://doi.org/10.1007/BF02113503
  15. Tetrahedron Lett. v.38 Streochemical studies of the skipped-polyol polyene macrolide class: Determination of mycoticin A and B Schreiber, S. L.;M. T. Goulet.
  16. Appl. Environ. Microbiol. v.50 Effect of phosphate and copper on the fermentation of hydroheptin Tunac, J. B.;L. E. McDaniel