• Korean Journal of Pharmacognosy
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• v.51 no.4
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• pp.302-309
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• 2020

The objective of this study was to optimize the condition for induction and proliferation of callus from Rhus chinensis Mill. and investigate the skin-brightening effect of Rhus chinensis callus (RCC). It was confirmed that the most proper plant growth regulator (PGR) for callus induction is 1.0 mg/L of 2,4-dichlorophenoxyacetic acid (2,4-D). The most optimal condition of PGR, medium and additives for callus proliferation were 2,4-D (1.0 mg/L), MS medium and citric acid, respectively. Inhibitory activities of tyrosinase were higher at 50 and 100 ㎍/mL of RCC extracts (41.86 and 75.56%, respectively) than arbutin (27.32%). As the results of measuring melanin inhibition in B16F1 melanocyte and B16F10 melanoma cell, RCC extracts increased its inhibitory activities concentration-dependently, and were found to have higher whitening effect than arbutin at a concentration of 100 ㎍/mL. Therefore, it is suggested that RCC can be used as an effective material for skin-brightening cosmetics.

• Journal of agriculture & life science
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• v.53 no.2
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• pp.15-26
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• 2019

An in vitro digestibility methods have been developed to mimic an in vivo system in the past decades. Because the in vivo techniques cause high cost, intensive labor, longer research periods and ethnical problems. In this review, the digestive systems from stomach to large intestine of pig as a monogastric animal were addressed to understand an in vivo digestion. The innovative in vitro technique using the Daisy II incubator was performed and the in vitro ileal and fecal dry matter (DM) digestibility of corn, rice, wheat and barley was determined.

• Korean Journal of Ichthyology
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• v.35 no.4
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• pp.354-360
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• 2023

The diet composition of Tanaka's snailfish, Liparis tanakae were collected by bottom trawl net in the Yellow Sea of Korea during 2022 (April, August, October, and November). The size of the specimens ranged from 3.8 to 52.2 cm in total length. L. tanakae were primary consumed caridea shrimps (Crangon hakodatei, Eualus spathulirostris, etc.) and specialist feeder mainly consumed caridea shirmps (C. hakodatei). The L. tanakae's trophic level is 3.63. Caridea shrimps were the primary prey items for all size groups (<20 cm, 20~30 cm, 30~40 cm, ≥40 cm). The proportion of E. spathulirostris has increased as the body size of L. tanakae increased, whereas the proportion of C. hakodatei decreased gradually. As the body size of L. tanakae increased the mean weight of prey per stomach (mW/ST) tended to increase significantly (One-way ANOVA, P<0.05). The caridea shrimps were the primary prey items throught all seasons. In summer, the main prey items were the C. hakodatei, however, in autumn, the proportion of C. hakodatei decreased while the proportion of E. spathulirostris increased.

• Journal of dental hygiene science
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• v.13 no.3
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• pp.347-353
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• 2013

This study was conducted to provide dental hygiene students with an opportunity which let them learn and have responsibility on bioethical perception by carrying out a survey for bioethical perception by personality types targeting 519 dental hygiene students. The study results were as follows: 1. Generally, in personality types, gender affects friendliness. Also, significant difference was shown in conscientiousness by age and practical experience. From finance point of view, significant difference was shown in neuroticism, extroversion, conscientiousness and openness, however, they did not satisfy test for equality of variances. 2. Generally, in bioethical perception, gender affects embryos right to life significantly (p <0.001) and considerable difference was appeared in intrauterine insemination by age. Significant differences were shown in embryos right to life by grade and whether students have practical experiences or not was the key factor which lead considerable difference in abortion. 3. In correlation of variances for personality types and bioethical perception, only friendliness and embryos right to life had weak correlation of 0.119. 4. In personality types affecting bioethical perception, friendlier personality had strong influence on embryos right to life. From this study, it can be concluded that bioethical perception could be affected by five personality types although they are not conclusive indicators which provide all the information.

• Microbiology and Biotechnology Letters
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• v.19 no.4
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• pp.343-347
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• 1991

An improved method for the selection of high tobramycin-producing mutants of Streptomyces tenebrarius ATCC 17920 was investigated. By the use of apramycin-containing media, low nebramycin-producing mutants were eliminated. Strain No. 23, resistant to apramycin and kanamycin B and sensitive to tobramycin, was isolated from soils, identified as Pseudomonas paucimobilis and used as a test organism for overlaying the mutants on agar plate. While inhibition zones were not shown when the parent strains were overlaid with soft agar containing the strain No. 23, clear zones were shown when high tobramycin-producing mutants were overlaid. Using this screening strategy, 58 mutants showing clear zones had been isolated. antibiotic activities of which were incresed to 3~8 fold compared to that of parent strain.

• Microbiology and Biotechnology Letters
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• v.13 no.3
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• pp.251-255
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• 1985

Nitrogen-fixing bacteria were isolated from the rice rhizosphere of various paddy fields in our country. The screening of 235 isolates for nitrogen-fixing ability resulted in the isolation of Enterobacter agglomerans NFB264 and three Klebsiella pneumoniae NFB 3, NFB 320. Plasmids of various molecular weight from 1.7 to more than 84 Mal. were detected by agarose gel electrophoresis in three out of four isolates. But, these plasmids had not any nitrogen-fixing genes. Hybridization experiments using Klebsiella pneumoniae M5al nitrogen-fixing genes, nif Q-K and nif DH, as probes revealed the presence of homologous sequences in the chromosomal DNA of all isolates. However the restriction patterns of nif genes of the isolates by various restriction endonucleases were different to those of Klebsiella pneumoniae M5al.

• Journal of agriculture & life science
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• v.44 no.2
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• pp.85-96
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• 2010

In this paper, we tried to investigate the adjusting patterns of production fluctuation of major soybean production countries. In particular, we focus our attention on the trade transmission effect of major soybean production countries on world market. We found that the instability of world market for soybean is likely to be increased mainly due to the production fluctuation and trade transmission effect of major production countries, e.g. Brazil, Paraguay, and Canada. Most of the major production countries have adjusted the production fluctuation through consumption and trade rather than inventory management, and hence the instability of world market for soybean tends to be more instable. Therefore we need to develop the effective policy measures for making domestic soybean market more stable such as a plan for keeping domestic production, a valid inventory management strategy, a use of futures market, and a strategy to diversify import market as a large importing country.

• Proceedings of the Korea Water Resources Association Conference
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• 2023.05a
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• pp.341-341
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• 2023

전 세계적으로 발생하고 있는 기후변화로 최근 극한의 가뭄이나 홍수가 발생하고 있으며, 이로 인한 경제·사회적으로 피해를 발생시키고 있는 실정이다. 이에 따라 기후변화에 직접적으로 영향을 주는 원인 중 하나인 온실가스에 대한 관심 높은 실정이며, 전 지구적으로 다양한 분야에서 탄소중립을 위한 정책이나 실천 방안 마련을 위한 연구가 활발히 이루어지고 있다. 우리나라에서는 2050 장기 저탄소 발전전략을 수립하였으며, 농축산부문의 온실가스 배출량을 2018년 배출량인 24.7백만톤 대비하여 2030년까지 27.1%인 약 6.7백만톤 감축을 목표로 하며 그 중 벼 논물관리를 통한 온실가스 감축 목표량은 540천톤으로 농업인의 참여가 요구된다. 이에 본 연구에서는 저탄소 논물관리를 위한 농업인 대상으로 총 3회의 교육을 실시하였다. 저탄소 논물관리 참여의식 고츼 및 탄소 중립에 대한 이해를 돕기위한 교육을 실시하였고 교육 전·후 참여 농업인 대상의 설문조사를 실시하였다. 저탄소 논물관리의 핵심인 중간 물떼기와 관련된 설문 결과, 교육 전 2주 미만 논물을 건조한다고 응답한 농업인은 51%였으나 교육 3회 실시 후 설문에서는 2주일 이상 논물을 건조한다고 응답한 농업인이 78%로 증가하였다. 또한, 출수기부터 완전 물떼기 전까지의 논물관리 방식인 걸러대기는 교육전 49%였지만 교육 실시 후 74%로 증가하여 걸러대기를 실천하는 농업인 비율이 높아졌다. 이처럼 농업분야의 온실가스 배출량 감축을 위한 정책이 현장에서 효과적으로 실천되기 위해서는 농업인의 참여가 필수적이다. 따라서 본 연구와 같이 농업인 대상의 교육이나 컨설팅 등이 함께 이루어진다면 더욱 높은 효과를 나타낼 수 있을 것으로 사료된다.

• Korean Journal of Organic Agriculture
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• v.31 no.1
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• pp.95-106
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• 2023

Entomopathogenic fungi are one of the microorganisms distributed worldwide, and they inhabit not only insects but also soils of various regions such as agricultural land and forest areas. In this study, entomopathogenic fungi distributed in the soil of forest areas in Korea were isolated using the insect-baiting method, and the relationship between the distribution and diversity of entomopathogenic fungi and the physical/chemical characteristics of the soil was analyzed. The soils were collected from five forest areas in Korea, and a total of 42 entomopathogenic fungal isolates were isolated from the collected soils. Among them, Beauveria bassiana (42.9%) and Metarhizium anisopliae (45.2%) were mainly isolated. In particular, it was confirmed that the distribution of M. anisopliae varies depending on the total nitrogen (g/kg) and organic matter content (%) of the soil. The soils in forest areas in Korea have a low pH of 4-5. Among all isolates, >50% of entomopathogenic fungi were isolated from silt loam. This study suggests that it will be helpful in understanding the relationship between the distribution and diversity of entomopathogenic fungi and the physical and chemical characteristics of soil.

• Journal of Life Science
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• v.31 no.6
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• pp.574-580
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• 2021

This study investigated the pathogenicity of different genotypes of infectious hematopoietic necrosis virus (IHNV) strains isolated from infected rainbow trout in Gangwon Province. RtPc0314c and RtPc0314g strains belonging to the JRt-Shizuoka lineage and RtPc0816g strain belonging to the JRt-Nagano lineage showed 100% cumulative mortality when inoculated at a high titer. In addition, more rapid necrosis was observed in rainbow trout infected with RtPc0314c and RtPc0314g mutations. When inoculated at a low titer, 100% mortality was not observed until the end of the experiment, but the mortality was higher in rainbow trout infected with a mutant strain belonging to the JRt-Shizuoka linage than a mutant strain belonging to the JRt-Nagano lineage. A histopathological analysis showed a clear signature of infection in kidney and spleen tissues upon infection with RtPc0314c and RtPc0314g but no signature of infection associated with the Rt03186 strain. Based on the results in this study, it seems that strains belonging to the JRt-Shizuoka lineage in Gangwon Province IHNV are more pathogenic.