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http://dx.doi.org/10.22889/KJP.2020.51.4.302

Induction and Proliferation of Callus in Rhus chinensis Mill. and Its Effect on Skin Whitening  

Kim, Dong-Myong (Biotechnology Research Institute, KJM Bio Ltd.)
Jung, Ju-Yeong (Biotechnology Research Institute, KJM Bio Ltd.)
Lee, Hyung-Kon (Biotechnology Research Institute, KJM Bio Ltd.)
Kwon, Yong-Seong (Biotechnology Research Institute, KJM Bio Ltd.)
Baek, Jin-Hong (R&D Center of Aloe, Kim Jung Moon Aloe Ltd.)
Lee, Kwan-Ho (Department of Chemical Engineering & Biotechnology, College of Engineering, Korea Polytechnic University)
Jang, Jin-Hoon (Department of Chemical Engineering & Biotechnology, College of Engineering, Korea Polytechnic University)
Han, In Suk (Department of Chemical Engineering, College of Engineering, The University of Utah)
Publication Information
Korean Journal of Pharmacognosy / v.51, no.4, 2020 , pp. 302-309 More about this Journal
Abstract
The objective of this study was to optimize the condition for induction and proliferation of callus from Rhus chinensis Mill. and investigate the skin-brightening effect of Rhus chinensis callus (RCC). It was confirmed that the most proper plant growth regulator (PGR) for callus induction is 1.0 mg/L of 2,4-dichlorophenoxyacetic acid (2,4-D). The most optimal condition of PGR, medium and additives for callus proliferation were 2,4-D (1.0 mg/L), MS medium and citric acid, respectively. Inhibitory activities of tyrosinase were higher at 50 and 100 ㎍/mL of RCC extracts (41.86 and 75.56%, respectively) than arbutin (27.32%). As the results of measuring melanin inhibition in B16F1 melanocyte and B16F10 melanoma cell, RCC extracts increased its inhibitory activities concentration-dependently, and were found to have higher whitening effect than arbutin at a concentration of 100 ㎍/mL. Therefore, it is suggested that RCC can be used as an effective material for skin-brightening cosmetics.
Keywords
Rhus chinensis Mill.; Plant tissue culture; Tyrosinase; Melanin; Skin-brightening;
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1 Kim, S. H., Park, H. H., Lee, S. Y., Jun, C. D., Choi, B. J., Kim, S. Y., Kim, S. H., Kim, D.K., Park, J. S. and Chae, B. S. (2005) The anti-anaphylactic effect of the gall of Rhus javanica is mediated through inhibition of histamine release and inflammatory cytokine secretion. Int. Immunopharmacol. 5: 1820-1829.   DOI
2 Lin, C. N., Chen, H. L. and Yen, M. H. (2008) Flavonoids with DNA strand-scission activity from Rhus javanica var. roxburghiana. Fitoterapia. 79: 32-36.   DOI
3 Chun, C. S., Kim, J. H., Lim, H., Sohn, H. Y., Son, K. H., Kim, Y. K., Kim, J. S. and Kwon, C. S. (2004) Antioxidative effect of Rhus javanica Linne extract against hydrogen peroxide or menadione induced oxidative stress and DNA damage in HepG 2 cells. Prev Nutr Food Sci. 9: 150-155.   DOI
4 An, D. S., Seo, S. J., Kim, N. W. and Lee, Y. S. (2017) Antiaging and anti-inflammatory activity of Rhus javanica branches extracts. J. Invest. Cosmetol. 13: 103-111.   DOI
5 Lee, Y. (1996) A colored book of the Korean plants. Kyo-Hak Pub, Seoul, Korea.
6 Yoo, S. J. (1972) Domestic resources of oriental drugs. Korean J. Pharmacogn. 3: 165-168.
7 Oh, S. T., Jung, H. S., Cho, M. J., Song, M. Y., Moh, S. H. and Seo, H. H. (2014) Effect of Artemisia annua Linne callus induced by plant cell culture technology on wound healing. J Korea Acad Industr Coop Soc. 15: 5628-5636.   DOI
8 Lee, N. N., Choi, Y. E. and Moon, H. K. (2015) Somatic embryo induction and plant regeneration from cold-stored embryogenic callus of K. septemlobus. J Plant Biotech. 42: 388-395.   DOI
9 Chenshu, A., Wang, X., Yuan, X., Zhao, B. and Wang, Y. (2003) Optimization of cryopreservation of Artemisia annua L. callus. Biotechnol. Lett. 25: 35-38.   DOI
10 Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.   DOI
11 Schenk, R. U. and Hildebrandt, A. (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot. 50: 199-204.   DOI
12 Lotan, R. and Lotan, D. (1980) Stimulation of melanogenesis in a human melanoma cell line by retinoids. Cancer Res. 40: 3345-3350.
13 Lloyd, G. and McCown, B. (1980) Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Proc. Intl. Plant Prop. Soc. 30: 421-427.
14 Pomerantz, S. H. (1966) The tyrosine hydroxylase activity of mammalian tyrosinase. J. Biol. Chem. 241: 161-168.   DOI
15 Mosmann, T. (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods. 65: 55-63.   DOI
16 Hosoi, J., Abe, E., Suda, T. and Kuroki, T. (1985) Regulation of melanin synthesis of B16 mouse melanoma cells by 1α, 25-dihydroxyvitamin D3 and retinoic acid. Cancer Res. 45: 1474-1478.
17 Jimenez-Cervantes, C., Solano, F., Kobayashi, T., Urabe, K., Hearing, V. J., Lozano, J. A. and Garcia-Borron, J. C. (1994) A new enzymatic function in the melanogenic pathway. The 5, 6-dihydroxyindole-2-carboxylic acid oxidase activity of tyrosinase-related protein-1 (TRP1). J. Biol. Chem. 269: 17993-18000.   DOI
18 De Leeuw, S. M., Smit, N. P., Van Veldhoven, M., Pennings, E. M., Pavel, S., Simons, J. W. and Schothorst, A. A. (2001) Melanin content of cultured human melanocytes and UV-induced cytotoxicity. J. Photochem. Photobiol. 61: 106-113.   DOI
19 Prota, G. (1980) Recent advances in the chemistry of melanogenesis in mammals. J. Invest. Dermatol. 75: 122-127.   DOI
20 Gamborg, O. L. C., Miller, R. A. and Ojima, K. (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151-158.   DOI
21 Kim, K. Y., Lee, S. J., Bak, E. Y., Jang, G. S. and Hwang, W. G. (2009) Effects of Galla rhois extracts and fractions on anti-oxidative acitivity and inhibition of melanin synthesis by melanoma cell. J. Kor. Soc. Cosm. 15: 1051-1058.
22 Iwata, M., Corn, T., Iwata, S., Everett, M. A. and Fuller, B. B. (1990) The relationship between tyrosinase activity and skin color in human foreskins. J. Invest. Dermatol. 95: 9-15.   DOI
23 Bell, A. A. and Wheeler, M. H. (1986) Biosynthesis and functions of fungal melanins. Annu. Rev. Phytopathol. 24: 411-451.   DOI
24 Chen, J. S., Wei, C. I. and Marshall, M. R. (1991) Inhibition mechanism of kojic acid on polyphenol oxidase. J. Agric. Food Chem. 39: 1897-1901.   DOI
25 Lerner, A. B. and Fitzpatrick, T. B. (1950) Biochemistry of melanin formation. Physiol. Rev. 30: 91-126.   DOI
26 Hwang, S. W., Nam, S. H., Lee, J., Kwon, H.S., Lee, K.D., Park, K. H. and Yang, M. S. (2005) Comparison of tyrosinase inhibitory effect of the natural antioxidants from Cedrela sinensis. Agric. Chem. Biotechnol. 48: 144-147.
27 Cho, Y. J., An, B. J. and Kim, J. H. (2011) Application of isolated tyrosinase inhibitory compounds from persimmon leaves. J. Life Sci. 21: 976-984.   DOI
28 Kwak, J. H., Kim, Y. H., Chang, H. R., Park, C. W. and Han, Y. H. (2004) Inhibitory effect of gardenia fruit extracts on tyrosinase activity and melanogenesis. KSBB Journal. 19: 437-440.
29 Ahn, J. H. and Min, Y. H. (2014) Inhibitory effects of apricot seed essential oil on melanogenesis. AJBC. 12: 677-683.
30 Maeda, K. A. and Fukuda, M. (1991) In vitro effectiveness of several whitening cosmetic components in human melanocytes. J. Soc. Cosmet. Chem. 42: 361-368.
31 Lee, I. S., Oh, S. R., Ahn, K. S. and Lee, H. K. (2001) Semialactone, isofouquierone peroxide and fouquierone, three new dammarane triterpenes from Rhus javanica. Chem. Pharm. Bull. 49: 1024-1026.   DOI
32 Wang, R. R., Gu, Q., Wang, Y. H., Zhang, X. M., Yang, L. M., Zhou, J., Chen, J. J. and Zheng, Y. T. (2008) Anti-HIV1 activities of compounds isolated from the medicinal plant Rhus chinensis. J. Ethnopharmacol. 117: 249-256.   DOI
33 Oh, J. Y., Choi, U., Kim, Y. S. and Shin, D. H. (2003) Isolation and identification of antioxidative components from bark of Rhus javanica Linne. Korean J. Food Sci. Technol. 35: 726-732.
34 Chung, S. C., Hwang, B. Y., Oh, G. J., Kang, S. J., Kim, M. J., Choi, W. H., Lee, K. S. and Ro, J. S. (1999) Chemical Components from the stem bark of Rhus javanica L. Korean J. Pharmacogn. 30: 295-300.