• Journal of Life Science
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• v.7 no.4
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• pp.297-308
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• 1997

Role of plant hormones on the leaf senescence of Phaseolus vulgaris were investigated by measuring the disassembly of chlorophyll-protein complexes in detached leaves treated with NAA, GA$_{3}$ , or BA. The loss of chlorophyll that was characteristic of leaf senescence induced disassembly of chlorophyll-protein complexed. During dark-induced senescence, PSI complex was rapidly degraded after the early stage, whereas RC-Core3 was slightly increased until the middle stage and slowly decreased thereafter. And gradual degradation of trimeric LCHII progressed after the late stage of senescence. Exogenous application of NAA and GA$_{3}$ had little or no effect in protecting disassembly of chlorophyll-protein complexes during leaf senescence compared to control. However exogenous BA application strongly leaves. In the simultaneous treatment of plant hormones and light, BA application under illumination of light was most effective in the stability of chlorophyll-protein complexes, particularly PSI, LHCII, RC-Core2, RC-Core3 and SC-1. these results suggest, therefore, that simultaneous application of BA and light induced synergistic effect on the stability off chlorophyll-protein complexes during leaf senescence.

• The Journal of the Korea Contents Association
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• v.19 no.7
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• pp.323-334
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• 2019

The purpose of this study is to propose agenda concerning the direction of agricultural science convergence research and development (R&D) under the fourth industrial revolution. For this study, we apply the Analytic Hierarchy Process (AHP) targeted at experts in the fields of agricultural academia and research operating R&D currently. Results suggest the following agendas; first, human resource training toward future is more emphasized rather than fragmentary technology innovation. Second, a flexible road map for agricultural science R&D need to be made for responding to short and long term issues relevant to the innovation. Third, mutual exchange and cooperative system need to be constructed between academia and research in order to create synergy effects. Finally, both institutional improvement and humanistic literacy should be emphasized for rapidly changing conditions and better human life under the fourth industrial revolution.

• The Optical Journal
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• s.131
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• pp.68-74
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• 2011

올해 정책자금 운영규모는 3조2075억원으로 지난해보다 2.3% 증가했다. 특히 기술혁신형 창업 및 성장초기 기업육성을 위해 '창업 및 개발기술사업화자금(1조4000억원)'을 지난해보다 2200억원 늘렸고, 개발기술사업화자금(2580억원)도 1000억원 늘렸다. 올해부터 정책자금의 배분전략이 그간의 "Negative시스템(특정산업을 제외한 모든산업지원방식)"에서 '전략산업'을 종점 지원하는 방식인 "Positive시스템"으로 전환된다. 중기청은 특히, 녹색 신성장동력 산업 등 성장 가능성이 큰 7대 전략산업에 정책자금의 70%인 2조2천억원을 집중 투입할 계획이다. 이에 따라 태양전지 LED응용 IT융합 고부가식품산업(녹색 신성장동력산업), 금형 등에 정책자금이 집중 지원될 것으로 보인다. 이와 함께 우량기업 및 한계기업을 지원대상에서 제외하고 동일기업에 대한 편중지원을 제한하는 등 정책목적성을 대폭 강화할 예정이다. 자체 신용으로 민간금융 이용이 가능하나 저금리 혜택을 위해 정책자금을 이용하는 우량기업 및 생명연장식 한계기업 등에 대한 지원을 배제하고, 절감된 재원(약 3,000억원)은 전략산업의 창업초기기업에 종점 지원할 계획이다. 자세한 사항 및 정책자금 신청 희망기업은 중소기업진흥공단 홈페이지(www.sbc.or.kr) '정책자금 융자도우미'를 통해 신청요건 및 추천자금 등을 자가진단 후 신청하면 된다.

• Journal of Life Science
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• v.26 no.3
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• pp.376-386
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• 2016

Apoptosis induction has been proposed as an efficient mechanism by which malignant tumor cells can be removed following chemotherapy. The intrinsic mitochondria-dependent apoptotic pathway is frequently implicated in chemotherapy-induced tumor cell apoptosis. Since DNA-damaging agent (DDA)-induced apoptosis is mainly regulated by the tumor suppressor protein p53, and since more than half of clinical cancers possess inactive p53 mutants, microtubule-damaging agents (MDAs), of which apoptotic effect is mainly exerted via p53-independent routes, can be promising choice for cancer chemotherapy. Recently, we found that the apoptotic signaling pathway induced by MDAs (nocodazole, 17α-estradiol, or 2-methoxyestradiol) commonly proceeded through mitotic spindle defect-mediated prometaphase arrest, prolonged Cdk1 activation, and subsequent phosphorylation of Bcl-2, Mcl-1, and Bim in human acute leukemia Jurkat T cells. These microtubule damage-mediated alterations could render the cellular context susceptible to the onset of mitochondria-dependent apoptosis by triggering Bak activation, Δψm loss, and resultant caspase cascade activation. In contrast, when the MDA-induced Bak activation was inhibited by overexpression of anti-apoptotic Bcl-2 family proteins (Bcl-2 or Bcl-xL), the cells in prometaphase arrest failed to induce apoptosis, and instead underwent mitotic slippage and endoreduplication cycle, leading to formation of populations with 8N and 16N DNA content. These data indicate that cellular apoptogenic mechanism is critical for preventing polyploid formation following MDA treatment. Since the formation of polyploid cells, which are genetically unstable, may cause acquisition of therapy resistance and disease relapse, there is a growing interest in developing new combination chemotherapies to prevent polyploidization in tumors after MDA treatment.

• Journal of Plant Biotechnology
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• v.44 no.1
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• pp.27-34
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• 2017

Our aim was to find the effective duration and culture conditions for microspore culture in a genetically variant cabbage (Brassica oleraceae L. var. capitata). We discovered that the '$2{\times}NLN$ medium containing $AgNO_3$ 1 mg/l, sucrose 13%' was most efficient in producing the cabbage embryos. The number of induced embryos was higher in $F_2$ or $F_3$ progeny generation than the $F_1$ hybrid cultivar used as plant materials. Thus, we recommend microspore culturing using progeny plants of failed $F_1$ cultivar. The acquisition ratio of embryos and plants derived from microspore was higher in the early flowering period as compared to the late flowering period. When transplanted in the soil, 71.2% plants developed from the early flowering period, compared to 27.0% from the middle period and 1.8% plants from the late period. Thus, we recommend microspore culture using buds collected during the early flowering period.

• Applied Biological Chemistry
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• v.37 no.3
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• pp.175-181
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• 1994

Six Korean medicinal herbs, Codonopsis lanceolata Traut (sasam), Platycodon grandiflorium A. De Candolle (gilkyung), Paeonia lactiflora var. trichocarpa Bunge (peony), Angelica gigas Nakai (danggwi), Astragalus membranaceus Bunge (hwangki) and Cnidium officinale Makino (chunkung) were added in brewing Korean traditional rice wine. Fermentation characteristics such as contents of total acid, reducing sugar, total sugar, pH, temperature, and alcohol concentration were investigated every 24 hr during fermentation periods. Changes of fermentation characteristics were remarkable in the initial period $(1{\sim}4\;days)$, slow in the middle $(5{\sim}7\;days)$, and almost unchangeable in the final $(8{\sim}10\;days)$. Total acid content was remarkably increased during the initial period but slowly afterwards. Reducing sugar content, total sugar content, and pH were remarkably decreased during the initial period but slowly afterwards. Alcohol concentrations of medicinal herb rice wines were $0.3{\sim}1.5%$ lower than the traditional wine throughout the fermentation periods. After the final period of 10 days of fermentation, alcohol concentration was observed as 12.6% in wine from sasam, 12.9% from gilkyung, 12.3% from peony, 12.4% from danggwi, 13.4% from hwangki, 12.6% in wine from chunkung, 13.6% in raditional wine respectively. Those differences were expected as the results of differences in various herbs. From the sensory evaluation, the highest score of overall quality was observed in the wine from hwangki, the next from chunkung, sasam, the traditewional wine, gilkyung, danggwi and the last from peony.

• Journal of The Korean Society of Grassland and Forage Science
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• v.42 no.4
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• pp.243-248
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• 2022

This study was conducted to evaluate the effect of feeding the whole crop rice silage (WCRS) which partially replaces the imported grass hay crop on the milking performance of Holstein and profitability. Two experimental diets were used according to the traditional practice of dairy farms. The control diet (C) was composed of 13kg/day of mixed hay, 6.8kg/day concentrate, and orchardgrass/bermudagrass hay (1.8kg/day). The treatment diet (T) was composed of 9.6kg/day of mixed hay, 6.8kg/day of concentrate and 2.2kg/day of WCRS. The CP content of the WCRS (4.6%) was lower than orchardgrass (11.3%) and bermudagrass hay (8.4%) due to the harvest being 30days later than the optimum harvest date of rice. The lower intake was observed in T during the entire experiment period which seems to be related to the higher proportion of fiber in rice (p<0.05). No significant difference was found in milk production between C (26.9kg) and T (26.3kg) during the entire experiment period (p>0.05). Also, no difference in milk fat, milk protein, total solids, and milk composition was observed between the two diets. Higher income per head was resulted in T (21,141won) compared to C (21,915 won). These results indicate that the partial replacement of the grass hay by the WCRS can bring the profit to the dairy farm due to the no difference between the control diet and the treatment diet.

• Journal of agriculture & life science
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• v.45 no.6
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• pp.135-140
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• 2011

This study was conducted to determine the effect of grape by-product on milk yield, milk composition and blood metabolites of lactating cows. A total of 8 Holstein dairy cows (average age = 89.9 months, average lactation day = 164.4, Reproduction number = 2.8) were randomly allocated into control (no additives, CON) or grape by-product treatment (add 1 kg of grape by-product per day, GBP) for 3 weeks. The concentrations of fat, protein, lactose and total solid in milk were not affected by grape by-product supplementation. However, milk yield (23.13 vs. 21.16 kg/d) was higher (p<0.05) in GBP than in CON, while SCC was lower (p<0.05). The concentrations of glucose, alanine aminotransferase, total protein and BUN in blood did not differ between treatments. But blood aspartate aminotransferase and total cholesterol concentrations (123.75 vs. 95.34 IU/L, 200.82 vs. 180.63 mg/dl) were higher (p<0.05) in cows fed GBP than CON. As the result, grape by-product, which has a various potential benefits, could be used as a functional diet supplement for lactating dairy cows.

• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.4
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• pp.339-346
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• 1999

This experiment was carried out to determine harvest date and cultivar effects on the agronomic characteristics, forage yield, and quality of fall sown oat (Avena sativa L.) at the forage experimental field, College of Agriculture and Life Sciences, Seoul National University, Suweon from August to November 1993. The experiment was arranged in a split plot design with three replications. The main plots were consisted of the harvesting dates, suchas early (11 October), mid(21 October), and late(1 November), the subplots were consisted of different maturities of oat cultivars, such as 'West', 'Cayuse' and 'Magnum'. The first heading date of an early maturing culivar, West, was observed on 10 October, and the heading increased up to 30% at the late harvesting date. The average dry matter (DM) content of oat cultivars increased significantly from 12.8 to 15.9% as the growing stage progressed(p<0.01). The average dry matter percentage of West, Cayuse, and Magnum was 15.4, 13.4, and 13.5%, respectively. The crude protein (CP) content of oat cultivars decreased significantly from 20.1 to 13.3% as the harvesting date was delayed(p<0.01), and the CP contents of midseason and late maturing cultivars, Cayuse and Magnum, respectively, were significantly higher than that of an early maturing cultivar West (p<0.01). The mid harvesting date showed the highest acid detergent fiber(ADF) and neutral detergent fiber (NDF) contents than the other harvesting dates, and an early maturing cultivar West had higher ADF and NDF contents than the midseason and late maturing cultivars, Cayuse and Magnum, respectively. The in vitro dry matter digestibility(IVDMD) of oat cultivar West decreased as the harvesting date was delayed, but that of Cayuse and Magnum decreased only by the mid harvesting date. Cayuse and Magnum showed 3~4% higher digestibility than West. The correlation coefficient between IVDMD and ADF content was -0.86. The average dry matter yield of oat cultivars increased from 2,901 to 5,901kg/ha as the harvesting date was delayed(p<0.01), but no significant difference was found among oat cultivars. The corrdelation coefficient between DM yield and gorwing degree days(GDD) was +0.86. The results of this study indicate that a delay in harvesting date would be recommendable for the increase of dry matter yield when fall-sown oat is grown. Especially, the DM yield of the midseason and late maturing cultivars, Cayuse and Magnum, respectively, can be increased without much change of forage quality although the harvesting date are delayed.

• Journal of The Korean Society of Grassland and Forage Science
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• v.14 no.3
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• pp.238-246
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• 1994

This experiment was carried out to determine the effects of seeding date and fall harvest method on the growth characteristics, forage yield and quality of winter rye(Seca1e cereale L.) at the forage experimental field, College of Agriculture and Life Sciences, SNU, Suweon from 5 September 1992 to 21 May 1993. The experiment was arranged in a split plot design with three replications. Main plots consisted of three harvests, no defoliation, cutting and grazing. Sub-plots consisted of seeding dates, early(5 September), mid(25 September) and late(l0 October). The results obtained are summari7d as follows; I . A 3-day difference in the first heading of rye was observed between no defoliation and fall grazing, while there was a 6day difference between early and late seeding dates. Therefore, the effect of seeding dates on the fist heading date of rye was larger than that of fall harvest methods. 2. CP content of rye with fall grazing was slightly higher averaged 16.5% compared with both fall cutting and no defoliated 1ye(15.6%). Mean CP content of rye at the early, mid and late seeding dates was 14.2, 14.5 and 19.1 %, respectively. 3. ADF content of rye was slightly higher with both fall cutting(29.99) and no defoliation(29.5%) than that of fall grazing(28.0%). ADF content of rye at the early, mid and late seeding dates was 30.7, 29.1 and 27.7%, respectively. Effect of fall harvest methods and seeding dates on NDF content of rye were similar to the observations made on ADF. 4. RFV and IVDMD of rye were lower with both no defoliation and fall cutting than fall grazing. but those of rye slightly increased with seeding dates progressed. 5. Dry matter yield of rye was the highest of 6,779 kg/ha with fall cutting, while the lowest yield of 6,240 kg/ha was obtained from no defoliation But no significant difference was found among the harvest methods. Dry matter yield of rye was significantly declined with later seedings. It is concluded that the highest forage yield of rye be possible from two harvest systems of fall and spring with earlier seeding rather than from one harvest system of spring.