• 가정의 벗
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• v.37 no.6 s.430
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• pp.4-5
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• 2004

선천성 기형의 종류는 매우 다양하며 완치가 가능한 기형도 많다. 그러므로 선천성 기형이 있다고 무조건 자랑할 필요는 없으며 전문가의 상담이 필요하다. 감기약 한번 잘못 먹어서, 또는 임신인 줄 모르고 한번 찍은 가슴 X-선 사진으로 인공임신중절을 하는데 이는 고쳐져야 할 관행이다.

• Microbiology and Biotechnology Letters
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• v.36 no.1
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• pp.6-11
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• 2008

To check the microbiological safety with respect to increased virulence of surviving pathogens after irradiation, in this study, the transcriptional change of vvp gene encoding metalloprotease, which is one of the typical virulence factors of Vibrio mulnificus, was monitored by real-time PCR during the course of growth cycle after reinoculation of irradiated Vibrio. When V. vulnificus was exposed to a dose of 0.5 and 1 kGy, the lag period before growth resumption of sub-cultures became longer than non-irradiated counterpart as increase of irradiation dose. In the case of non-irradiated culture, the transcription of vvp was significantly activated at 15 h after inoculation, when bacterial growth reached the stationary phase, and the highest level of pretense activity (686 U/mL) was measured at the same time. Interestingly, vvp expression of irradiated Vibrio was turned up earlier than non-irradiated Vibrio during the mid log phase of growth, whereas these rapid induction of vvp expression from irradiated cells didn't result in an increase of metalloprotease production. When Vibrio was irradiated at 0.5 and 1 kGy, the protease activities peaked at 18 h after inoculation and the levels of activities were lower 1.2- and 1.4-fold, respectively, compared to the non-irradiated counterpart. Results from this study indicate that gamma radiation is not likely to activate the virulence ability of surviving Vibrio.

• Biomedical Science Letters
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• v.3 no.1
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• pp.21-28
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• 1997

Male rats of Albino strain were divided into four groups, control group, X-irradiated group, WR-2721 treatment group and X-irradiated group treated with WR-2721. The radioprotective effect of treatment with S-2 (3-aminopropylamino)ethylphosphorothioic acid (WR-2721) in the dose of 200mg/kg by intraperitonial injection on rats 20min prior to wholebody X-irradiation (8Gy) was studied. Each group determined serum alkaline phosphatase (ALP), aspartate aminotransferase (ASI), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) activities and contents of serum glucose after 1, 3, 7 and 10 days. The ALP and AST activities of X-irradiated group were significantly decreased (p<0.05) compared with that of control group, but X-irradiated group treated with WR-2721 less decreased those enzyme activities compared with the X-irradiated group. X-irradiated group was significantly increased (p＜0.05) ALT and LDH activities compared with that of control group, but X-irradiated group treated with WR-2721 less increased those enzyme activities compared with the X-irradiated group. The concentration of serum glucose of X-irradiated group was significantly increased (p<0.05) compared with that of control group, but X-irradiated group treated with WR-2721 less increased compared with that of X-irradiated group. It may be considered that WR-2721 provided radioprotective effect of organs of body from X-irradiation.

• Journal of Life Science
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• v.21 no.6
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• pp.893-900
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• 2011

Prostatic acid phosphatase (PAP) is one of the widely used biomarkers in the diagnosis of prostate cancer. It was initially identified in 1935 and is the most abundant phosphatase in the human prostate. PAP is a prostate-specific enzyme that is synthesized in prostate epithelial cells. It belongs to the acid phosphatase group that shows enzymatic activity in acidic conditions. PAP is abundant in prostatic fluid and is thought to have a role in fertilization and oligospermia. It also has a potential role in reducing chronic pain. But one of the most apparent functions of PAP is the dephosphorylation of macromolecules such as HER-2 and PI3P that are involved in the ERK1/2 and MAPK pathways, which in turn leads to inhibition of cell growth and tumorigenesis. Currently, clinical trials using PAP DNA vaccine are underway and FDA-approved immunotherapy using PAP is commercially available. Despite these clinically important aspects, molecular mechanisms underlying PAP regulation are not fully understood. The promoter region of PAP was reported to be regulated by NF-${\kappa}B$, TNF-${\alpha}$, IL-1, androgen and androgen receptors. Here, the features of PAP gene and protein structures together with the function, regulation and roles of PAP in prostate cancer are discussed.

• Journal of Life Science
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• v.17 no.2 s.82
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• pp.260-265
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• 2007

This study was carried out to investigate the effect of Electron Beam irradiation on physico-chemical characteristics of Hanwoo meat. A total of sir beef carcasses $(280{\sim}300\;kg)$ that were quality grade $1^+$(marbling score No. 7, meat color No. 4, maturity No. 1, texture No. 1) was purchased at the commercial slaughter house. The carcasses were transported and washed using high pressure water, and pasteurized with 50% ethyl alcohol in the laboratory. After the carcasses were deboned and trimmed, loin and round were taken out to make steak (1.5 cm thickness) or patty respectively. Samples were wrap or vacuum packaged and irradiated with 0, 3, 4.5, 6 and 7.5 kGy using electron-beam accelerator. Irradiated samples were used to measure pH, moisture, crude protein, crude fat, and meat color. There was no significant (p>0.05) difference in pH between vacuum packaged (VP) and wrap packaged (WP) treatment, and the pH was not changed by electron-beam irradiation levels. Both control and irradiated treatments of steak showed higher tendency in moisture content. In crude protein content, control was higher than irradiated treatment in steak, but there were no difference in patty. Lightness ($L^{\ast}$) of meat color has no difference between irradiated and non-irradiated treatment (p>0.05). The value of redness and Yellowness of meat was dropped by increasing irradiation (p<0.05), but there was no difference between control and 3 kGy treatment (p<0.05).

• Proceedings of the Korea Contents Association Conference
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• 2005.11a
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• pp.43-47
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• 2005

With the high interest and the results of active research in life science and Bioinformatics recent years, various information and resources have been produced and new algorithms have been developed to analyze those. Web Services is a computer technology using XML, SOAP, WSDL and UDDI. This paper introduces the One Stop Web Services program developed in CCBB which has a graphic user interface for users.

• Proceedings of the Korean Information Science Society Conference
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• 2005.11b
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• pp.223-225
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• 2005

근래 들어 생명과학 및 생명정보에 대한 관심이 높아지면서 활발한 연구 활동의 결과물로 많은 정보를 얻을 수 있게 되었다. 또한 방대한 생명정보를 분석할 수 있는 많은 알고리즘이 개발되었으며 웹 기술이 발달함에 따라 생명정보의 활용도가 높아져서 다양한 방법으로 정보서비스가 가능하게 되었다. 특히 XML, SOAP, WSDL, UDDI를 사용하는 Web Services는 분산 컴포넌트 기반의 컴퓨팅 기술로 최신의 기술이다. 이에, 바이오인포매틱스센터에서는 CCBB, NCBI, EBI, DDBJ 등의 사이트에서 배포한 Web Services에 접근하여 입력 값과 출력된 결과를 확인 및 유지하고, 객체들의 흐름을 볼 수 있는 프로그램으로 One-Stop Web Services를 개발하였다. One-Stop Web Services는 GUI 환경의 인터페이스로 사용자에게는 용이한 실행 환경을 제공하므로, Web Services 개발자에게 사용자 인터페이스 개발 부담을 줄일 수 있다.

• Biomedical Science Letters
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• v.3 no.2
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• pp.107-114
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• 1997

Recent reports indicate that the clinical usefulness of prostate specific antigen (PSA), particulary in the differentiation of benign prostate hyperplasia from prostate cancer, can be improved by measuring the amount of free PSA in serum. Measuring free PSA is especially useful in attempts to improve diagnositc performance of PSA in the diagnostic gray zone of total PSA. The objective of this study was to develop free PSA assay kit using sandwich microplate enzyme immunoassay format. We chose a test format with polyclonal anti-PSA antibodies coated on the wells and monoclonal anti-free PSA antibodies for quantification to gain higher test sensitivity. We adpoted 10 uL of specimen and 2 hours of first incubation time with detecting antibody for free PSA EIA format using microplate. The within-day and between-day precision (%CV) in the high and low concentration ranges were below 4%. The correlation coefficient between in-house free PSA assay and commercial assay kit was r=0.9965 (slope=0.0984, y intercept=0.0173, N=27). No hook effect was found by 40 ng/mL and correlation coefficient (r) value of the fitted linear regression was over 0.995. The recovery tests were in the range of 98.9∼104.1％ for free PSA. In conclusion, in-house free PSA enzyme immune assay is cost effective, simple and rapid and could be useful for the prognosis after theraphy as well as for the differential diagnosis between prostate cancer and benign prostate hyperplasia.

• Journal of Life Science
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• v.12 no.4
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• pp.469-476
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• 2002

The DNA topoismerase I inhibitor $\beta$-lapachone, the product of a tree from South America, is known to exhibit various biological properties, however the mechanisms of which are poorly understood. In the present report, we investigated the effects of $\beta$-lapachone on the growth of human prostate carcinoma DU-145 cells. Upon treatment with $\beta$-lapachone, a concentration-dependent inhibition of cell viability was observed and cells developed many of the hallmark features of apoptosis, including condensation of chromatin and DNA fragmentation. Flow cytometry analysis confirmed that $\beta$-lapachone increased populations of apoptotic-sub Gl phase. In addition, proteolytic cleavages of poly (ADP-ribose) polymerase (PARP) and $\beta$-catenin protein were observed after treatment of $\beta$-lapachone. These apoptotic effects of $\beta$-lapachone in DU-145 cells were associated with marked induction of Bax protein, however the levels of Bcl-2 expression were decreased in a dose-dependent manner.

• Journal of Life Science
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• v.13 no.5
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• pp.569-575
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• 2003

Supercritical $CO_2$ extraction was applied to extraction of the valuable compound for benign prostatic hyperplasia from saw palmitto. Three levels of parameters, temperature $(35^{\circ}C,\; 60^<\circ}C)$, pressure 22∼30 Mpa and ethanol modified solvent concentration, were used as the independent variables of central composite to rotate the design. The extracting pressure and temperature interaction was significantly affected the extraction of valuable compounds. The ethanol was an efficient modifier to extract valuable compound by supercritical carbon dioxide. The optimal processing conditions were extracting pressure, 30 Mpa, extracting temperature, $60^<\circ}C$, ethanol modifier, 15% for extracts of the valuable compounds from saw palmitto. In conclusion, it was found that supercritical $CO_2$ extraction can successfully be applied to extract high quality extracts from saw palmitto.