• Title/Summary/Keyword: 상호교배

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Development of a New Strain and Mating Type Analysis by Inbreeding Method in Flammulina velutipes (근연간 교배방법에 의한 팽이 탄핵균주의 교배형분석 및 균주 육성)

  • Kong, Won-Sik;Cho, Yong-Hyun;Kim, Gyu-Hyun;Jhune, Chang-Sung;You, Chang-Hyun;Yoo, Young-Bok;Kim, Kwang-Ho
    • Journal of Mushroom
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    • v.1 no.1
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    • pp.9-14
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    • 2003
  • To breed new superior strains, collected strains were characterized and then several white strains were selected as parents. Monokaryons from the parents were isolated and studied. All tested white strains showed same mating genotype. Growth rate of monokaryons were lower than collected dikaryons. New dikaryotic strains were derived from inbreeding method, which means mating between monokaryotic isolates from different white strains having same mating genotype. Some of them showed higher yields of fruitbody than their parents. Specially Fv 4-1 strain showed the best productivity. Furthermore some mating combination showed cytoplasmic effect, when they mated reciprocally.

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Pollen Characteristics of Flower Type and Cross Compatibility with Table Grape in Vitis amurensis Rupr. (왕머루의 화형에 따른 화분특성 및 포도의 교배화합성)

  • Park, Young-Sik;Kim, In-Jong;Jeong, Byeong-Chan;Heo, Jae-Yoon;Park, Sung-Min
    • Korean Journal of Medicinal Crop Science
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    • v.13 no.6
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    • pp.234-240
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    • 2005
  • This study was conducted to identify cross compatibility with table grape and pollen characteristics by flower type in Vitis amurensis Rupr. (GW-22, GW-45, GW-56, GW-200, GW-202 and GW-300) collected in Gangwon area. Flower structure, germinability and morphology of pollen, and cross compatibility with table grape were examined. Flowers of GW-22 and GW-56 had an aborted gynoecium and five erect fdaments, and all of them was male. The percentage of pollen germination of GW-22 and GW-56 on the agar medium was 27.6-29.8%. Flowers of GW-45, GW-200, GW-202 and GW-300 had well-developed pistils and five stamens with curled filaments. The percentage of pollen germination of female plant on the agar medium was 0%. Pollen grail)s from GW-22 and GW-56 (male flower) have symmetrically distributed three furrows on their surface and each furrow contains a germ pore at the equatorial level (Tricolporated). GW-45, GW-200, GW202, and GW-300 (Female flower) produced pollen grains with no germinative structures (acolporated). In self-pollina-tion of female flowers, berries of clusters were no berry and no seed. Therefore, flower structure in V. amurensis proved to be that of other dioecious Vitis species. In cross combination of female and male flower of V. amurensis made normal seeds. In cross combination of table grape and V. amurensis of male flower made normal seeds. But, in cross combination of female flower of V. amurensis did not make seeds. Therefore, V. amurensis was cross compatible with vitis spp, and V. amurensis varieties may contribute to breeding studies of Vitis spp.

예비구조설계에서의 상호작용방식에 따른 컴퓨터 활용방안

  • 정종현
    • Computational Structural Engineering
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    • v.12 no.1
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    • pp.86-94
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    • 1999
  • 예비구조설계에서는 구조시스템 대안의 생성과 발전, 여러 방법과 대상에 따른 해석과 설계 다양한 요구조건과 구조적 특성을 고려한 여러 구조시스템 대안의 비교 및 선택 과정을 거친다. 그리고, 이러한 각 과정은 구조설계자의 경험적 지식을 바탕으로 하는 종합적인 사고와 판단에 따라서 단계적, 반복적으로 이루어진다. 그러므로, 예비구조설계에서는 정형화된 자료와 작어뿐 아니라 비정형화된 자료와 작업도 처리해야 한다. 따라서, 컴퓨터는 정형화된 자료와 작업을, 구조설계자는 비정형화된 자료와 작업을 직접 처리하고 이를 상호작용을 통하여 교환 및 지시하는 방식, 즉 구조설계자와 컴퓨터의 역할분담에 기초한 상호작용방식을 통하여 예비구조설계에 컴퓨터를 효율적으로 활용할 수 있을 것이다. 구조설계자와 컴퓨터의 역할분담에 기초한 상호작용을 원활히 지원하기 위해서는 구조시스템 대안의 기하학적 향상, 재료에 대한 자료를 효과적으로 제시할 수 있는 3차원 관점, 각 구조시스템 대안을 검토하고 비교하는 사항이 되는 다양한 요구조건에 대한 자료들을 관리하고 제시할수 있는 요구조건 관점이 필요하다. 그리고 예비 구조설계가 진행되어 온 과정, 방향, 설계의도등의 파악을 위한 바탕이 될 수 있는 작업과정관점, 구조시스템 대안 발전 관점이 필요하다. 예비구조설계의 프로세스 조절을 위한 상호작용을 효과적으로 지원하기 위해서는 컴퓨터가 수행 할 수 있는 정형화된 작업들을 예비구조설계의 진행순서인 구조시스템 대안의 생성과 발전, 구조시스템 대안의 해석과 설계, 구조시스템 대안의 선택에 따라서 나열하여 제시할 필요가 있다. 이를 통해서 구조설계자는 자신의 판단에 따라서 다음에 수행해야 할 작업을 결정하고, 그에 해당하는 정형화된 작업을 컴퓨터가 제시한 작업 중에서 선택하고 그 수행을 지시하여 자신의 경험적 지식과 설계의도에 맞추어 예비구조설계의 프로세스를 조절할 수 있다.정조합력은 유의차가 컸으나, 상반조합 능력은 없었다. 교배친의 우성효과는 컸다. 잡종강세 환경변이 및 상가적 작용도 컸다. 우성의 방향은 정의 방향이었으므로 우성귀전자가 크게 작용하였다. 이들 형질들의 귀전자들은 초우성을 나타내었다. 교배친의 자견층중의 우성순서는 잠120>잠114>잠108>잠119>잠118>잠107>잠117>잠113 순이었고, 웅견층중에서는 잠114>잠108>잠120>잠117>잠118>잠107>잠119>잠119>잠113 순이었다. 자견층 비율에서는 광의의 귀전력이 협의의 귀전력보다 컸고, 웅견층 비율에서는 같았다, 견층 비율에서는 일반조합 능력은 크게 나타났으나, 특정조합 능력과 상반조직 능력은 나타나지 않았다. 자견층 비율에서 교배친의 우성효과는 컸다. 자견층 비율에서는 교배친의 우성효과는 적었다. 자웅견층 비율의 잡종 강세는 적게 나타났다. 환경변이와 상가적 작계는 자웅견층 비율에서는 크게 나타났다. 우성의 방향은 자견층 비율에서는 정의 방향으로 우성 귀전자가 크게 작용하였으며, 자견층 비율에서는 정의 방향으로 우성 귀전자가 부분적으로 작용하였다. 교배친의 자견층 비율의 우성순서는 잠117>잠114>잠108>잠120>잠118>잠119>잠107>잠113 순이었고, 자견층 비율에서는 잠114>잠117>잠108>잠118>잠107>잠119>잠113>잠120의 순이었다.지방산의 조성이 많은 차이를 보였다.{2+}$ 26 및 $Na^+$ 26 mg $L^{-1}$이었다. 양액 재배 후 버려지는 폐양액 중의 무기성분 함량은 양액재배에 이용되는 원수에 비해 상당히 높아졌다.료로서 응용 가능성이 있음을 시사한다.약재료인 약초류 등을 이용하였는데 오랫동안 푹 삶아 그물에 곡류 등을 넣어 죽이나 밥으로 조리하였으며 면으로도 조리하였다. 이상과 같이 조선시대 주식류의 종류 및 조리방법에 대한

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Effects of Sublethal Doses of Chlorpyrifos-methyl on the Following Generation of the Beet Armyworm, Spodoptera exigus (Hubner) (아치사량의 chlorpyrifos-methyl이 파밤나방(Spodoptera exigua(Hubner)) 차세대형성에 미치는 영향)

  • 이준익;김용균
    • Korean journal of applied entomology
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    • v.36 no.3
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    • pp.277-282
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    • 1997
  • Chemosterilant effect of chlorpyrifos-methyl (CPM) on the beet armyworm, Spodoptera e.rigua (Hubner) was investigated, using topical application. Egg fecundity and fertility were analyzed in the adults reared from the fifth instar larvae which were treated with sublethal doses of CPM. Though CPM reduced fecundity a little, it gave significant negative effect on egg fertility in a dose-dependent manner. Genetic analysis was performed by reciprocal crosses between treated (100 Wgllarva) and untreated individuals and showed a dominant lethal effect of CPM on egg fecundity and fertility. This results indicate that chlorpyrifos-methyl may act as a chemosterilant as well as a well-known neurotoxicant, and suggest that it can be used in genetic control program.

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Studies on Breeding and Cultivation Characteristics of Lentinula edodes Strains for Sawdust Cultivation (표고 톱밥재배용 균주의 육종과 재배특성에 관한 연구)

  • Ryu, Sung-Ryul;Bak, Won-Chull;Koo, Chang-Duck;Lee, Bong-Hun
    • The Korean Journal of Mycology
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    • v.37 no.1
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    • pp.65-72
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    • 2009
  • For the breeding purpose, in this study we attempted to select a new Lentinula edodes strain by comparing mycelial growth rate, fruit-body quality and mushroom productivity per substrate weight of hybrid strains with parent strains. Nineteen hybrid strains were made by cross of di-karyotic and mono-karyotic to develop strains suitable for sawdust cultivation of L. edodes. Among the nineteen strains, ten were selected by comparing mycelial growth rate on PDA media and wood rot on sawdust-based substrates. The most mushroom harvesting was achieved by 100 days incubation from KFRI 960, KFRI 961, KFRI 962, KFRI 963, KFRI 964, KFRI 971, KFRI 973 and 125 days from KFRI 968, 970. Experiment of incubation periods with the ten strains showed that there were productivity differences between 100 and 150 days incubations. Through comparison of hybrid strains' mushroom productivity it was found that there was no difference in mushroom productivity through crossing high temperature types with high temperature types, crossing low temperature types with high temperature types and crossing unknown temperature types with high temperature types, but there was difference through crossing middle temperature types with high temperature types. Hybrid strains showed better productivity compared with parent strains. Fruiting temperature type analysis of hybrid strains confirmed that strains of high temperature types have a dominant character.

Interspecific crossabilities among wild mulberry species and cultivars growing in the Korean Peninsula (한반도에 자생하는 야상종 상호간 및 야상 화분을 재배 3품종에 인공교배를 한때의 임성)

  • 박광준
    • Journal of Sericultural and Entomological Science
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    • v.43 no.2
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    • pp.88-92
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    • 2001
  • The crossabilities among 3 wild mulberry species and 3 cultivars growing in the Korean Peninsula had been studied. The result of the crossabilities of Moms tiliaefolia with Moms bombycis and 3 cultivars were possible, but with Morus mongolica was impossible. Those of Moms mongolica with Moms bombycis, Morus tiliaefolia and 2 cultivars except Daeryukppong were possible too. And those of Morus bombycis with Moms titiaefolia, Moms mongolica were impossible, but with 3 cultivars were possible moderately.

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Optical Interconnection Applied by Genetic Algorithm (유전 알고리즘을 적용한 광 상호연결)

  • Yoon, Jin-Seon;Kim, Nam
    • Journal of the Korean Institute of Telematics and Electronics D
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    • v.36D no.7
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    • pp.56-65
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    • 1999
  • In this paper, a pixelated binary phase grating to generate $5{\times}5$ spots in designed using simple Genetic Algorithm(sGA) composed of selection, crossover, and mutation operators, and it can be applied for the optical interconnection. So as to adapt that GA is a robust and efficient schema, a chromosome is coded as a binary integer of length $32{\times}32$, the ranking method for decreasing the stochastic sampling error is performed, and a single-point crossover having $16{\times}16$ block size is used. A designed grating when the probabillty of mutation is 0.001, the probability of crossover is 0.75 and the population size is 300 has a 74.7[%] high diffraction efficiency and a $1.73{\times}10^{-1}$ uniformity quantitatively.

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Expression in Successive Generations of bar Gene Introduced in Petunia (Petunia에 도입된 bar Gene의 세대진전에 따른 발현 양상)

  • Ha, Young-Min;Park, Sang-Mi;Kim, Zhoo-Hyeon
    • Journal of Plant Biotechnology
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    • v.31 no.4
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    • pp.261-266
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    • 2004
  • This experiment was carried out to confirm the stability of bar gene introduced into petunia plant through Agrobacterium-mediated transformation, in successive generation, or after crossing or back-crossing. Some of different 25 transgenic plants were used in crossing and back-crossing to wild type, or repeated-selfing to T$_4$ generation. On the processing of experiment, it was found that some lines lost their resistant ability to herbicide basta, or showed non-Mendelian segregation mode: produced much more susceptible segregants than resistant plants. Even though there are exceptional cases, which was off from expected, the genetic stability of bar gene introduced could be confirmed strongly, because in almost case, the segregation of resistant and susceptible plants to basta was done under Mendelian-law according to single gene dominant model.

Stability of Dicofol Resistance of the Two-spotted Spider Mite, Tetranychus urticae Koch (Acarina: Tetranychidae) (Dicofol 저항성 점박이응애의 안정성)

  • Kim, Gil-Hah;Song, Cheol;Chang, Bu-Young;Park, No-Joong;Cho, Kwang-Yun
    • Korean journal of applied entomology
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    • v.34 no.1
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    • pp.61-64
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    • 1995
  • Stability of resistance of the two-spotted spider mite (Tetranychus urticae) to dicofol was investigated. Resistance level (23.0-fold) of adult females to dicofol was kept up to 1 year without selection by dicofol, but was decreased to 15.8-, 10.7-fold after 2 and 3 years, respectively. Susceptibility of adult females obtained from reciprocal crosses $(R_{female}\timesS_{male},R_{male}\times S_{female})$ of the R and S stains to dicofol was declined by the success of generation.

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Incompatibility Factors and Genetic Analysis of Pleurotus sajor-caju (여름느타리버섯의 교배계(交配系) 및 교배균주(交配菌株)의 유전(遺傳) 분석(分析))

  • Go, Seung-Joo;Shin, Gwan-Chull
    • The Korean Journal of Mycology
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    • v.14 no.1
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    • pp.17-23
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    • 1986
  • The mating system of monokaryotic isolates in Pleurotus sajor-caju was controlled by two incompatibility factors A and B of tetrapola mating system. The mycelia of dikaryotic isolates grew faster than those of their component monokaryons, but no correlation between dikaryotic and their component monokaryotic isolates was found. The primodia formed well on the potato dextrose agar (PDA) media not only in the dikaryotic isolates but also in the monokaryons under irradiated conditions. The dikaryotic isolates produced normal sporophores; however, the monokaryotic isolates produced abnormal sporophores when they were cultivated with sawdust substrates. Some dikaryotic isolates derived by mating between monokaryotic isolates showed high yields of sporophores more than those of parental strains. Both the dikaryotic mycelial growth rate and primodia formation number on the PDA plate showed significant correlation with its sporophore products on sawdust substrates.

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