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http://dx.doi.org/10.5010/JPB.2004.31.4.261

Expression in Successive Generations of bar Gene Introduced in Petunia  

Ha, Young-Min (경상대학교 원예학과, 농업생명과학연구원)
Park, Sang-Mi (경상대학교 원예학과, 농업생명과학연구원)
Kim, Zhoo-Hyeon (경상대학교 원예학과, 농업생명과학연구원)
Publication Information
Journal of Plant Biotechnology / v.31, no.4, 2004 , pp. 261-266 More about this Journal
Abstract
This experiment was carried out to confirm the stability of bar gene introduced into petunia plant through Agrobacterium-mediated transformation, in successive generation, or after crossing or back-crossing. Some of different 25 transgenic plants were used in crossing and back-crossing to wild type, or repeated-selfing to T$_4$ generation. On the processing of experiment, it was found that some lines lost their resistant ability to herbicide basta, or showed non-Mendelian segregation mode: produced much more susceptible segregants than resistant plants. Even though there are exceptional cases, which was off from expected, the genetic stability of bar gene introduced could be confirmed strongly, because in almost case, the segregation of resistant and susceptible plants to basta was done under Mendelian-law according to single gene dominant model.
Keywords
Agrobacterium; back-cross; basta herbicide; F2; generation; hybrid; stability; transformation;
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Times Cited By KSCI : 3  (Citation Analysis)
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