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Utilization of Bombus terrestris as a Sweet Cherry Pollinator in Rain-sheltered Growing (생식용 체리 비가림 재배시 서양뒤영벌(Bombus terrestris L.)의 화분매개 곤충 활용)

  • Kwack, Yong-Bum;Kim, Hong-Lim;Choi, Young Hah;Lee, Jae Han
    • Journal of Bio-Environment Control
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    • v.21 no.3
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    • pp.294-298
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    • 2012
  • In sweet cherry (Prunus avium L.) growing there are several severe problem which have to be overcome to produce highly graded fruits because of fruit rots and fruit crackings, if there is frequent precipitation during immature fruit step and picking season. In order to reduce fungicide sprayings and produce qualified fruits in areas with rainy season like as South Korea, rain-sheltered growing is necessary absolutely. Sweet cherry blooms early to medium April in southern area of South Korea. If we depend on honeybees (Apis mellifera) distributed in natural ecosystem, it is not easy to get normal fruit-set every season because of low temperature around blooming time. And also bee keepers seldom sell honeybee hives as a pollinator during spring, instead they keep honeybee hives to get honey. Recently use of B. terrestris as a pollinator of cherry tomato, oriental pumpkin etc. grown in protected cultivation system increase abundantly. Therefore, in this study we studied B. terrestris as an alternate of honeybee to pollinate sweet cherry grown in rain shelter. In part of foraging activity B. terrestris shows staying on a cherry flower for about six second and visiting frequency of 11 flowers per minute. However A. mellifera stayed about 15 second on a flower and visited 4~5 flowers per minute. There were no significant difference in fruit-setting rate and fruit characteristics after using B. terrestris and A. mellifera as pollinators of sweet cherry. Consequently there is no negative effect when we use B. terrestris as an alternate pollinator of A. mellifera in sweet cherry cultivation under rain shelter.

Residue of Combined Insecticide of Polynactin Complex(Tetranactin) and BPMC in Apple and Soil (Polynactin Complex (Tetranactin)와 BPMC 혼합제의 사과 및 토양 잔류성)

  • Yoon, Jae Cheon;Lee, Seok Joon;Park, Jong Woo;Kim, Jang Eok
    • Current Research on Agriculture and Life Sciences
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    • v.11
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    • pp.101-110
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    • 1993
  • The residues of combined insecticide of polynactin complex(tetranactin) and BPMC were determined to establish an index for the safety use to apple. Evaluation was made on residual concentration of tetranactin and BPMC in apple as a function of application frequency and date when the combined insecticide of tetranactin and BPMC was sprayed into apple. Their persistence in soil were also studied under the field and laboratory conditions. Recovery percentage from apple was ranged from 74.0 to 77.5 in tetranactin, 87.1 to 83.6 in BPMC, those from soil was 82.3 to 88.4 in tetranactin, 83.6 to 887.1 in BPMC. The minimum detectable limits of tetranactin and BPMC were 0.01ppm in apple pulp and 0.03ppm in apple peel and soil. The residue percentage of tetranactin and BPMC in the peel and pulp part of apple was about 96 in peel part by five sprays up to 3th day before harvest. The residues of tetranactin and BPMC in apple are proved to 0.39ppm and 0.75ppm by five sprays up to 30days before harvest. Maximum residue limit(MRL) of BPMC for fruits was established of 0.3ppm in Environment Protection Agency of Korea, and thus it is suggested that the preharvest intervals of combined insecticide for apple could be 30 days with twice spray. The half life of tetranactin in soil under field and laboratory conditions was 6.9 and 24.4 days, and in case of BPMC was 6.3 and 23.2 days, respectively.

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A Possible Mechanism Related with Non-spinning Syndrome of Bombyx mori that Intimidates the Sericultural Industry in Northern Kyungbuk (경북 북부지역의 양잠산업에 피해를 주고 있는 누에(Bombyx mori) 미화용 기작에 관한 연구)

  • Kim, Yong-Kyun;Bae, Sang-Ki;Lee, Sun-Young;Ji, Dong-Jin;Kim, Jin;Hong, Yong-Pyo;Kim, Gil-Ho
    • Korean journal of applied entomology
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    • v.43 no.2
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    • pp.143-153
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    • 2004
  • Non-spinning syndrome of Bombyx mori has been serious issue in sericulture industry near Kyungbuk area. This study was focused on the analysis of the mechanism and on screening candidate chemicals inducing the anti-metamorphosis of the silkworms. Rearing temperatures or initial body weight of the final instar larvae did not affect a normal larval to pupal metamorphosis of B. mori. However, pyriproxyfen (a juvenile hormone (JH) agonist) induced follicle patency significantly even at its 10$\^$-8/ M concentration and inhibited metamorphosis of B. mori in both developmental time and dose dependent manners. Pyriproxyfen induced JH esterase (JHE) activity and downregulated expression of JH binding protein of 5. mori. These results suggests that pyriproxyfen induced JHE activity as a JH agonist and that the elevated JHE activity degraded endogenous JH and resulted in JHBP gene expression. Based on the fact that the JH agonist induced follicle patency and inhibited metamorphosis of B. mori, follicle patency bioassay suggested that three commercial pesticides including simazine, molinate or alachlor were proved to give potent JH agonistic effect on B. mori. Further direct exposure experiments to these candidates are required to determine the chemicals responsible for the non-spinning syndrome of 8. mori.

Rapid diagnosis and control of sulfonylurea resistant Monochoria korsakowii (Sulfonylurea계 제초제 저항성 물옥잠의 조기진단과 방제)

  • Park, Tae-Seon;Moon, Byeong-Chul;Cho, Jeong-Rae;Kim, Chang-Suk;Kim, Moo-Sung;Kim, Kil-Ung
    • The Korean Journal of Pesticide Science
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    • v.8 no.1
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    • pp.63-70
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    • 2004
  • Sulfonylurea(SU)-resistant Monochoria korsakowii has resently been found in rice fields in Korea. A quick, practical and accurate test of confirming herbicide resistance is necessary to take timely management decision. This article describes a rapid reliable assay to detect SU-resistant biotype of Monochoria korsakowii. Up to now, the resistance to SU has been usually checked by application seedlings with herbicide. This application technique is time consuming and not practical. Therefore, we have developed efficient, rapid and practical diagnosis which allow easy detection of the SU-resistant Monochoria korsakowii by survival rate and regenerated plant length to herbicide application after cutting plants $0.5\sim1cm$ from the planted surface. This new rapid diagnosis can determine the SU resistance of the Monochoria korsakowii within 7 days at least. If the resistance of Monochoria korsakowii is identified by the rapid diagnosis, the selection of herbicide according to the stages of plants for the effective control is very important. The resistant biotype which treated with SU herbicide-based mixtures survived from the fields could effectively be controlled by soil application of butachlor+pyrazolate GR or by foliar application of the mixtures of bentazone SL and 2,4-D SL.

Residue Determination of Chlorothalonil in Sesame and Soil (Chlorothalonil의 참깨 및 토양중 잔류소장에 관한 연구)

  • Lee, Kang-Bong;Cho, Ill-Kyu;Shim, Jae-Han;Suh, Yong-Tack
    • Korean Journal of Environmental Agriculture
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    • v.9 no.1
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    • pp.15-22
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    • 1990
  • The efects of application time on the persistence of chlorothalonil in sesame plants and its persistence in soil were studied in the field. A procedure was used which permited the analysis of residues of Chlorothalonil (DACONIL 2787) in sesame(Sesamum indicum L.)and soil. The procedure was based on the multiresidue analytical method using an polarized acetonitrile solution or acidified acetone as the extracting solvent. The DACONIL 2787 is separated from the fat in the extracts by using an activated alumina and Florisil chromatogaphic column. Residues of DACONIL 2787 were determined by subjecting the alternate eluate from the Florisil column to gas chromatographic analysis(ECD). Residue of chlorothalonil in sesame are proved to 0.06 ppm for six sprays, 3rd day before harvest The half-life of chlorothalonil in soil proved to be 8.9 days and residues of chlorothalonil was only 0.03 ppm 100 days after application of 15 ppm.

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Studies on Mechanism and Damage Occurrence for Foliar Spray of NaCl Solutions in Fruit Trees (NaCl 엽면살포에 따른 과수의 피해양상과 기작에 관한 연구)

  • Kim, Seung-Heui;Song, Gi-Cheol;Park, Jeong-Gwan;Park, Moo-Yong
    • Korean Journal of Environmental Agriculture
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    • v.25 no.4
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    • pp.352-358
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    • 2006
  • We investigated the mechanism on occurring of briny injury in four fruit species (apple, pear, grape, peach). Briny injury of apple, pear, peach, and grape was induced by the foliar splay of saline solutions. Browning symptom was initiated one hour after the spray of 3% NaCl in apple, pearl and peach trees. while two hour were needed for grape, but the latter developed the symptom faster than the formers. Foliar spray of 3% NaCl resulted in 4-fold increase in ethylene production 24 h after the spray, and the production was returned to early stage rate after 72 h. Ethylene production of control was nearly constant with slight decrease after 4 h. Browning and defoliation response to the spray of 3% NaCl on lower side were not different from the spray on both sides regardless of fruit species, while no injury symptoms occurred by the spiny on upper side. In saline damage for treated date, lateral bud was only germinated in apple at 30 July. A shoot apex was 100% grew in all fruit tree. In result for hormonal content of leaf and shoot apex, control was high t-zeatin content in leaf. However, IAA and ABA content was increasing in high saline concentration.

Response of Red Sorrel (Rumex acetosella L.) to Several Soil- and Foliar-Applied Herbicides (애기수영의 화학적 방제를 위한 제초제 선발 및 선발 제초제의 살초효과)

  • Kim, Song-Mun;Kim, Yong-Ho;Hwang, Ki-Hwan;Ahn, Mun-Sub;Hur, Jang-Hyun;Han, Dae-Sung
    • The Korean Journal of Pesticide Science
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    • v.3 no.3
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    • pp.45-53
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    • 1999
  • Red sorrel (Rumex acetosella L.) is a troublesome perennial weed in the alpine grasslands of Kangwon Province of Korea. A number of soil- and foliar-applied herbicides were evaluated for their efficacy of red sorrel control. In greenhouse experiments, no soil-applied herbicides, such as pendimethalin, simazine, alachlor, metolachlor, ethalfluralin controlled red sorrel, however, foliar-applied herbicides, such as glufosinate, paraquat, glyphosate, glyphosate + 2,4-D, dicamba, mecoprop, 2,4-D, bentazone controlled more than 60% of red sorrel 2 weeks after treatments. When dicamba and 2,4-D were applied to red sorrel in different growth stages, the auxin-type of herbicides cold control red sorrel regardless of growth stage. This result implies that the auxin-type herbicides can be applied between early May (early growth stage) and mid lune (before fruit maturation) at Kangwon alpine grasslands. In a field experiment, glufosinate at 1.0 kg a.i, $ha^{-1}$, glyphosate at 3.28 kg a.i. $ha^{-1}$, dicamba at 0.96 kg a.i. $ha^{-1}$, and mecoprop at 150 kg a.i. $ha^{-1}$ controlled more than 80% of red sorrel 4 weeks for treatment, suggesting such herbicides could be applied for red sorrel control at Kangwon alpine grasslands.

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Monitoring of Toxicants Affecting Metamorphosis of Silkworm in Mulberry Leaves (뽕잎 및 대기 중 누에영향물질 모니터링 및 독성조사)

  • Park, Kyung-Hun;Park, Yeon-Ki;Lee, Hee-Dong;Park, Byung-Jun;Joo, Jin-Bok;Sohn, Bong-Hee;Kang, Pil-Don;Kim, Mi-Ja;Seong, Su-Il
    • Journal of Sericultural and Entomological Science
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    • v.46 no.2
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    • pp.58-64
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    • 2004
  • The current study was performed to clarify the pupation disorder symptoms in silkworm which herbicide, molinate has been regarded as a causal origin. Molinate residues in mulberry leaves was monitored in major rearing area of silkworm; Yeacheon, Sangju and Namwon. Detected range of the molinate in mulberry leaves was 0.002 to 0.013 mg/kg and detection frequency was 30.0 to 81.8%. Molinate concentration in the air of mulberry tree growing area was $0.004\;{\mu}g/m^3$. Water diluted solution of molinate at the concentration of 1, 10, 100 mg/l gave no abnormal symptoms in silkworm irrespective of spraying to silkworm or spiking to diet. Exposing the silkworm to the air containing 250, 2,500, and $10,000\;{\mu}g/m^3$ also revealed no abnormal symptom. These results illustrated that the abnormal growth of silkworm encountered in the field was not caused by pesticide contamination.

Production Enhancement of Benzophenanthridine alkaloids in the Suspension Cultures of California poppy using Cyclodextrin (양귀비 세포 현탁배양계에서 Cyclodextrin을 이용한 Benzophenanthridine alkaloids의 생산성 증대)

  • 박세춘;조규헌
    • KSBB Journal
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    • v.11 no.4
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    • pp.411-419
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    • 1996
  • In this research, an extractive production system for alkaloids, where production and some degree of separation occur simultaneously, was developed in a way that the fast removal of alkaloid produced from the suspension cultures was done by capturing alkaloid with cyclodextrins. The alkaloid production was substantially enhanced up to 40 fold when the solid cultures of E. califonica cells treated with ${\beta}$-cyclodextrin compared to the control. The enhancement of alkaloid production was also observed in the suspension cultures. Interestingly, the production pattern seemed to change when the cultures were treated with ${\beta}$-cyclodextrin so that the major part of the alkaloids in the treated cultures was present in the medium, while the non-treated cultures produced the alkaloids intracellularly. ${\beta}$-cyclodextrin was the most effective one in terms of the alkaloid production among the cyclodextrilns(${\alpha}$-cylodextrin, ${\beta}$-cyclodextrin and ${\gamma}$-cyclodextrin) tested in the suspension cultures. ${\beta}$-cyclodextrin showed no adverse effect on the cell growth. The most effective concentration of ${\beta}$-cyclodextrin was observed around 1.5% (w/v) in the suspension cultures. The formation of the inclusion complex of the alkaloids with ${\beta}$-cyclodextrin in the suspension cultures was confirmed by detecting the shift of UV absorbance from 274 nm to 282 nm with a UV spectrophotometer.

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Prediction of Time to Corrosion for Concrete Bridge Decks Exposed to De-Icing Chemicals (제빙화학제 살포로 인한 콘크리트 교량 바닥판의 철근부식 시작시기의 예측)

  • Lee, Chang-Soo;Yoon, In-Seok;Park, Jong-Hyok
    • Journal of the Korea Concrete Institute
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    • v.15 no.4
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    • pp.606-614
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    • 2003
  • The major cause of deterioration for the concrete bridge decks exposed to de-icing chemicals would be chloride-induced reinforcement corrosion. Thus, in this paper, in order to predict time to corrosion for concrete bridge decks in the urban area, chloride concentration was measured with depth from the surface. A frequency analysis on surface chloride concentration and chloride diffusion coefficient of concrete bridge deck equals 0.192, 29.828 in the scale parameter and 7.899, 1.983 in the shape parameter of gamma distribution. The average value of surface chloride concentration equals 1.5 kg/㎥ and condenses from 1 to 2 kg/㎥ in the level of probability 70%. From the probabilistic results, it is confirmed that 26mm of minimum cover depth in order to target 20 years over is calculated. The countermeasure strategy to extend the service life of concrete bridge deck exposed to de-icing chemicals would be an effective method to increase cover depth and to place high performance concrete, which could lead to reduce the chloride diffusion coefficient and distribution range.