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A Possible Mechanism Related with Non-spinning Syndrome of Bombyx mori that Intimidates the Sericultural Industry in Northern Kyungbuk  

Kim, Yong-Kyun (Department of Agricultural Biology, Andong National University)
Bae, Sang-Ki (Department of Agricultural Biology, Andong National University)
Lee, Sun-Young (Department of Agricultural Biology, Andong National University)
Ji, Dong-Jin (Department of Agricultural Biology, Andong National University)
Kim, Jin (Central Research Institute, Kyung-Nong Corporation)
Hong, Yong-Pyo (Department of Applied Chemistry, Andong National University)
Kim, Gil-Ho (Department of Sericulture & Entomology, Sangju National University)
Publication Information
Korean journal of applied entomology / v.43, no.2, 2004 , pp. 143-153 More about this Journal
Abstract
Non-spinning syndrome of Bombyx mori has been serious issue in sericulture industry near Kyungbuk area. This study was focused on the analysis of the mechanism and on screening candidate chemicals inducing the anti-metamorphosis of the silkworms. Rearing temperatures or initial body weight of the final instar larvae did not affect a normal larval to pupal metamorphosis of B. mori. However, pyriproxyfen (a juvenile hormone (JH) agonist) induced follicle patency significantly even at its 10$\^$-8/ M concentration and inhibited metamorphosis of B. mori in both developmental time and dose dependent manners. Pyriproxyfen induced JH esterase (JHE) activity and downregulated expression of JH binding protein of 5. mori. These results suggests that pyriproxyfen induced JHE activity as a JH agonist and that the elevated JHE activity degraded endogenous JH and resulted in JHBP gene expression. Based on the fact that the JH agonist induced follicle patency and inhibited metamorphosis of B. mori, follicle patency bioassay suggested that three commercial pesticides including simazine, molinate or alachlor were proved to give potent JH agonistic effect on B. mori. Further direct exposure experiments to these candidates are required to determine the chemicals responsible for the non-spinning syndrome of 8. mori.
Keywords
Bombyx mori; Juvenile hormone; JH binding protein; JH esterase; Non-spinning syndrome; Pyriproxyfen;
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