• Journal of dental hygiene science
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• v.10 no.5
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• pp.365-372
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• 2010

Caesalpinia sappan L. has long been commonly used in oriental folk medicines and as dyes materials. To investigate the antioxidative activities and antiproliferation effects of brazilin from C. sappan heart wood, the MeOH soluble extract was successively fractionated by using hexane, $CHCl_{3}$, EtOAc, BuOH, MeOH, and $H_{2}O$. In these fractions, we were purified brazilin from EtOAc fraction which partitioned to 3.94% of the highest yields. The effects of brazilin and the extracts on human oral carcinoma cells (KB) by MTT assay and their antioxidant activities by DPPH, TCA assay and Fenton reaction were tested. The results showed that the brazilin could inhibits the proliferation of KB cells and obviously decreased the production of nitric oxide of the cells. When the concentration of the brazilin reached to $100\;{\mu}g/ml$, the inhibition percentage of the cell growth was about 60%. In assay on antioxidant activities, The results showed that brazilin exhibit the highest capacity of DPPH free radical scavenging effects among tested extracts. When the concentration of brazilin reached to 1 mg/ml, the lipid peroxide inhibition and radical inhibition activities were determined to be 65.0% and 85.8%, respectively. These results are suggest that brazilin have stronger antiproliferation effect on KB cell and antioxidant properties.

• Food Science and Preservation
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• v.17 no.6
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• pp.861-866
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• 2010

We obtained hot-water extracts (HWE) and 70% (v/v) ethanol extracts (EE) from cultured wild ginseng roots (CWGR) and determined the saponin and total polyphenol contents, and antioxidant activities. The yields of freeze-dried powder from the HWE and EE were 27.86% and 18.33% (both w/w), respectively. The total polyphenol content of the EE (22.63 mg/g) was higher than that of the HWE (17.90 mg/g). Ginsenoside-Rb1 and -Rg1 contents of hot-air-dried CWGR were 17.90 mg/g and 22.63 mg/g, respectively. The electron-donating ability of HWE and EE were 2.82-60.58% and 3.88?70.88%, respectively, and the reducing powers ($OD_{700}$) were 0.02-0.17 and 0.07-1.90, respectively, at concentrations of 1-20 mg/mL. Thus, the HWE reducing power was markedly lower than that of the EE, but the SOD-like activity of the EE was significantly higher than that of the HWE. The nitrite-scavenging activities of HWE and EE were 9.25-19.18% and 11.94-53.49%, respectively, at concentrations of 1-20 mg/mL. Additionally, the TBARS (Thiobarbituric acid reactive substances, % value) of the EE (1-20 mg/mL) was 9.18-66.59%, thus 1.9-2.8-fold greater than that of the HWE (4.74-24.88%). In conclusion, we provide experimental evidence that extracts of CWGR may be natural antioxidants.

• Food Science and Preservation
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• v.24 no.6
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• pp.786-794
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• 2017

This study analyzes the chemical composition and thiobarbituric acid reactive substance levels of Godulbaegi (Youngia sonchifolia M.) depending on collection time. The moisture and crude fat content in leaf and root decreased, while crude fiber, crude protein, carbohydrate, and ash increased with increases in collection time. The mineral elements tended to increase in each sample with increases in collection time. The content of vitamin B increased as collection time increased. Vitamin C content was approximately five times higher in the leaves than that in the roots. Total amino acids in leaf and root increased considerably as collection time increased content of phenolic compounds in root were higher than that in the leaf and these contents increased. Antioxidant activity of Godulbaegi was higher in the root than in the leaf and increased as collection time increased.

• Journal of Life Science
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• v.17 no.11
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• pp.1533-1538
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• 2007

This study was carried out to determine the inhibitory effects of solvent extracts from Sargassum hemiphyllum on growth of cancer cell lines (AGS human gastric adenocarcinoma and HT-29 human colon cancer cells) and production of lipid peroxides. Inhibitory effects of acetone with methylene chloride extract from S. hemiphyllum on the growth of AGS and HT-29 cancer cells were increased as dose dependent patterns (p<0.05). The methanol extract was more effective on inhibition of growth of AGS. The treatments of hexane, 85% aq. methanol, butanol and water fractions significantly inhibited the growth of cancer cells (p<0.05) and the inhibitory effect was stronger in HT-29. In DCFH-DA (dichlorodihydrofluorescin diacetate) assay, acetone with methylene chloride and methanol extracts showed a stronger inhibitory effect on the production of cellular lipid peroxides (p<0.05) compared with the butanol and hexane fractions. These results indicate that the consumption of S. hemiphyllum may be recommended as a potent functional food for preventing cellular oxidation and cancer.

• Journal of Environmental Science International
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• v.11 no.10
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• pp.1109-1116
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• 2002

This study analyzed concentrations of volatile organic compounds (VOCs) produced from incineration of papers at $600^{\circ}C$. The papers used in this study included A4 papers (new, printed with ink-jet, printed with carbon), newspapers (printed with bean oil, printed with a general newspaper ink), packaging box, document envelope, single-use paper cup, and cosmetic tissue. Papers were heated from room temperature upto $600^{\circ}C$ providing air inside of the electric furnace and then they were oxidized for 80 minutes at $600^{\circ}C$ maintaining the same air supply. VOCs emitted from the incineration process were sampled using an air sampling pump and bags for 160 minutes and then the components and concentrations of the VOCs were analyzed by a CC-MS. The most prominent chemical structure of the Vous identified from incineration of the papers was furans and then furans were followed by aromatics and aliphatic alkenes. About 40% of the identified VOCs contained double bonds, which have relatively a high ozone (ground level) formation potential, within their molecular structure. Also, some cancer suspecting compounds like benzene, dichlorormethane and chloroform were identified.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2002.02a
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• pp.282-288
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• 2002

본 연구는 상업용 규모의 쇄미선별공정에 사용할 수 있는 파이로트 규모 원통형 홈 선별기 시작기의 설계 및 제작에 필요한 기초 연구로서, 실험실용 원통형 홈 선별기를 이용하여 쇄미선별 실험을 실시하였다. 원통 회전속도, trough각도, indent 크기와 형상, 공급량을 요인으로 하여 완전립의 수거율과 순도, 쇄립의 수거율과 순도 및 선별효율을 조사하였다. 수행한 연구결과를 요약하면 다음과 같다. 1. 선별효율에 대한 원통 회전속도와 trough각도의 단독효과 및 교호작용은 1% 수준에서 유의성이 인정되었다. 원통 회전속도가 커지면trough각도 역시 그에 따라 적절히 증가시켜야만 선별효율의 저하가 방지되는 것으로 나타났다. 2. 최고 선별효율 값은 홈의 모양과 크기, 그리고 공급량에 관계없이 낮은 회전속도 (17rpm)와 중간 trough각도 (37.5$^{\circ}$또는 60$^{\circ}$)가 조합된 처리에서 나타났으며, 60~70% 범위의 높은 값을 보였다. 선별효율에 관한 원통 회전속도와 trough각도의 최적 조합은 17rpm, 37.5$^{\circ}$라고 판단된다. 3. 말발굽형 홈과 반구형 홈 간 선별효율의 차이는 없었다. 따라서, 실제 상업용 규모의 원통형 홈 쇄미선별기 개발에 있어서는 제작이 쉽고 유지.보수가 간편한 반구형 홈을 채택하는 것이 바람직할 것으로 생각된다. 길이 2.5mm이하의 미립인 쇄미의 선별에 사용할 홈의 크기는 2.5mm 보다는 약간 큰 3.0mm 정도가 되어야만 할 것으로 판단된다. 4. 공급량에 따른 선별효율의 차이는 1% 수준에서 유의성이 인정되었으며, 공급량이 작았을 때 전반적으로 선별효율이 높았다.타리 시프터를 채택, 사용하고 있었으며, 로타리시프터 사용상의 문제는 회전몸체를 지지하는 rod spring의 파손 등 구조와 관련된 것이었다. 로타리 시프터에 의한 쇄립의 선별과 제거정도는 만족할 만한 수준은 아니었다. 4. 국내 유통백미 완전립의 길이, 폭, 두께는 각각 5.02mm, 2.93mm, 2.03mm이었으며, 산물밀도와 천립중은 각각 745.3kg/m3 및 20.46g이었다. 5. RPC 백미제품의 품질경쟁력 향상을 유도하고자 현행 쇄미의 정의와 기준을 보다 강화하여 다음과 같은 쇄미 기준과 계급을 설정, 제시하였다. "완전립" - 길이가 3.75mm이상인 미립 "준완전립" - 길이가 2.5∼3.75mm인 미립 "쇄미" - 길이가 1.75∼2.5 mm인 미립 "이물" - 길이가 1.75mm이하인 미립.볼 때 상토 종자혼합비 6 : 1, 성형롤 회전속도 약 7 rpm으로 판단되며, 이 때 제조능력은 시간당 약 65 Kg(펠렛종자 약 39,000 개), 성형률 약 87 %, 종자손실률은 약 30 %, 펠렛종자 내 평균 종자수는 약 5.5 개, 완전 벼 종자 3개 이상 포함 펠렛종자 비율은 약 100 %가 될 것으로 보인다. 세포의 Androgen 생성을 촉진시키는 역할이 있는 것으로 보여진다 따라서 옻나무 유래 F는 포유동물의 생식기능에 중요하게 작용하는 것으로 사료된다.된다.정량 분석한 결과이다. 시편의 조성은 33.6 at% U, 66.4 at% O의 결과를 얻었다. 산화물 핵연료의 표면 관찰 및 정량 분석 시험시 시편 표면을 전도성 물질로 증착시키지 않고, Silver Paint 에 시편을 접착하는 방법으로도 만족한 시험 결과를 얻을 수 있었다.째, 회복기 중에 일어나는 입자들의 유입은 자기폭풍의 지속시간

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.40 no.12
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• pp.1063-1069
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• 2012

In present paper, we used magnesium alloy having a lower density and higher electrical conductivity for bipolar plate to reduce the weight of PEM fuel cell. The silver was coated to prevent corrosion and form passivation film on the metal surface with sputtering. In acid proof evaluation for setting optimal coating conditions, the homogeneity of coating thickness was improved by coating with the thickness of 3 ${\mu}m$ which not indicated any micro cracks and the temperature $180^{\circ}C$. The performance test and evaluation based on the clamping pressure and channel depth to determine the configuration of bipolar plate for assembling single cell was implemented. And then we assembled single cell with this bipolar plate and implemented the performance test to ensure and compare the current-voltage performance followed as several factors such as coating or non-coating, the change of clamping pressure, the change of channel depth, etc. As these results, the maximum power density of single cell with the coated bipolar plate was 192 $mW/cm^2$ and it was confirmed that the power density per unit mass was better than existing metal bipolar plate.

• Journal of the Korean Applied Science and Technology
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• v.31 no.3
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• pp.440-445
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• 2014

The purpose of this study was to investigate anti-inflammatory and antioxidant effects of essential oil from seed of Zanthoxylum schinifolium on cultured RAW 264.7 cell line. Antioxidant activity of essential oil was evaluated by two different assays as 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and super oxide dismutase (SOD) like activities. This essential oil was tested for cell viability on RAW 264.7 cell line by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. The effects of anti-inflammatory on LPS-induced RAW 264.7 cell line was studied by the content of nitric oxide (NO) and prostagladin $E_2$ ($PGE_2$) in cells. The essential oil of seed from Zanthoxylum schinifolium obtained dose-dependently increased the scavenging activity on DPPH radical scavenging activity and SOD like activity. The essential oil showed low cytotoxicity as more than 98% cell viability in $40{\mu}g/mL^{-1}$ concentration. The essential oil of seed extracted from Zanthoxylum schinifolium presented obvious effect on inflammation. These results suggest that essential oil of seed from Zanthoxylum schinifolium may have value as the potential anti-inflammatory effects by decreasing the action of NO and $PGE_2$ and preventing the activation of oxidative.

• Journal of the Korean Society of Food Science and Nutrition
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• v.12 no.4
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• pp.316-322
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• 1983

In order to obtain elementary data of enzymatic browning of apples and apple products and to examine effectively inhibitory method of browning, we extracted polyphenol oxidase (EC 1.10.3.1) from apple (Ralls Janet) and investigated its general properties. The optimum conditions for the enzyme reaction were pH 6.0 and temperature of $30^{\circ}C$. The enzyme was very stable at pH 4.0, and at the range of pH 5.0-9.0 its activity was above 80% compared with pH 4.0. The enzyme was very stable by heating at $40^{\circ}C$ for 1 hour, and almost 50% of enzyme activity was lost by heating at $60^{\circ}C$ for 30 minutes. The polyphenol oxidase activity was enhanced by the addition of $Cu^{2+}$ and $Mn^{2+}$, respectively, meanwhile $Na^+$, $Hg^{2+}$ and $Co^{2+}$ inhibited the enzyme activity. The enzyme activity was greatly decreased in the presence of inhibitors such as cysteine, sodium metabisulfite and ascorbic acid. The polyphenol oxidase greatly catalyzed the oxidation of o-diphenols such as chlorogenic acid and catechol, which suggests that main substrate of polyphenol oxidase is o-diphenol compounds.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.71-71
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• 2019

고사리(Ferns)는 고사리 속(Pteridium spp.)에 속하는 양치식물의 총칭으로서 우리나라에는 22과 70속 272종이 분포되어 있는 것으로 보고되었다. 고사리의 어린 순에는 가식부 100 g 당 칼슘 15.0mg, 칼륨 185.0mg 등이 함유되어 있으며 골다공증, 심혈관질환 등에 효과가 있고 식이섬유로 인해 변비 예방에도 유용한 것으로 알려져 있다. 고사리 뿌리에서 추출한 전분은 중국 및 일본 등지에서 면류나 제과용으로 이용되고 있어 고사리 재배의 부가가치를 향상시킬 수 있는 방안으로 기대되고 있다. 본 연구에서는 고사리 뿌리는 가정용믹서기로 분쇄하는 방법과 고무망치를 사용해 파쇄한 방법에서 식품첨가제로 이용되고 있는 sodium metabisulfite 등 3종의 산화방지제를 사용하여 추출한 전분의 이화학적 특성을 조사하였다. 고사리 전분의 추출 수율은 분쇄하는 방법에서 높은 경향이었고 추출용액 중에서는 sodium metabisulfite 4% 용액에서 분쇄 처리 9.5%와 파쇄 처리 8.3%로 가장 높은 수율을 보였고, ascorbic acid 침지에서는 5.1%와 5.5%로 낮은 수율을 나타냈다. 추출한 전분의 색도값에서는 파쇄하는 처리에서 L 값이 높아지고 a와 b 값은 낮아지는 경향이었고 sodium metabisulfite 4% 용액 침지에서 분쇄와 파쇄 두 방법에서 가장 높은 명도값을 보였다. 고사리 전분의 추출방법별 Ca 함량은 분쇄 처리에 파쇄 처리에서 높아지는 양상을 보였고 파쇄 후 ascorbic acid 4% 용액에서 추출하는 처리에서 0.23%로 가장 높았으며, Mg 함량도 분쇄 처리에 비해 파쇄 처리에서 경미하게 높은 양상을 보였다. 유효태 Fe 함량 또한 파쇄처리에서 높은 경향을 보였고 추출 용액중에서는 ascorbic acid 4% 용액이 0.12%의 높은 함량을 보였다. 고사리 전분의 추출방법별 호화특성에서 최고 점도는 증류수, ascorbic acid 및 sodium carbonate 추출에서는 파쇄하는 방법에서 높아지는 경향이었지만 sodium metabisulfite 추출에서는 감소하는 경향을 보였다. 강하점도는 모든 추출용액에서 분쇄 처리에 비해 파쇄 처리에서 현저하게 증가하는 양상을 보였고 치반점도는 sodium metabisulfite와 ascorbic acid 추출은 파쇄처리에서 83.9 RVU와 53.0RVU로 낮아지는 경향을 보인 반면 sodium carbonate와 증류수 추출에서는 증가하는 양상을 나타냈다. 호화시간은 분쇄 처리가 파쇄 처리에 비해 약간 늘어나는 경향이었고 추출용액 간에는 큰 차이가 없었으며 호화온도는 분쇄처리가 파쇄 처리에 비해 높아지는 결과를 나타냈으며 파쇄한 고사리 뿌리를 증류수를 이용해 추출한 전분에서 가장 낮은 $69.7^{\circ}C$의 호화온도를 보였다.