Browse > Article
http://dx.doi.org/10.12925/jkocs.2014.31.3.440

Anti-oxidant and Anti-inflammatory activities of Zanthoxylum schinifolium Essential Oil  

Kim, Bo-Ae (College of Sciences & Technology, Division of Biomedicinal & Cosmetics, Mokwon University)
Publication Information
Journal of the Korean Applied Science and Technology / v.31, no.3, 2014 , pp. 440-445 More about this Journal
Abstract
The purpose of this study was to investigate anti-inflammatory and antioxidant effects of essential oil from seed of Zanthoxylum schinifolium on cultured RAW 264.7 cell line. Antioxidant activity of essential oil was evaluated by two different assays as 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and super oxide dismutase (SOD) like activities. This essential oil was tested for cell viability on RAW 264.7 cell line by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. The effects of anti-inflammatory on LPS-induced RAW 264.7 cell line was studied by the content of nitric oxide (NO) and prostagladin $E_2$ ($PGE_2$) in cells. The essential oil of seed from Zanthoxylum schinifolium obtained dose-dependently increased the scavenging activity on DPPH radical scavenging activity and SOD like activity. The essential oil showed low cytotoxicity as more than 98% cell viability in $40{\mu}g/mL^{-1}$ concentration. The essential oil of seed extracted from Zanthoxylum schinifolium presented obvious effect on inflammation. These results suggest that essential oil of seed from Zanthoxylum schinifolium may have value as the potential anti-inflammatory effects by decreasing the action of NO and $PGE_2$ and preventing the activation of oxidative.
Keywords
essential oil; Zanthoxylum schinifolium; antioxidant; anti-inflammatory;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 H. K. Seong, Y. H. Lee and C. K. Seong, Herbal home remedies. Happy Blue. Seoul, Korea, 69 (2012).
2 J. W. Lee, Volatile flavor components of Korean sancho fruit and tree (Zanthoxylum schinifolium). Korean J. Community Nutr., 11, 493 (1998).
3 S. J. Lee, Korean folk medicinemonographs series. No. 3. Publishing center of Seoul National University, Seoul, Korea, 88 (1996).
4 M. S. Jung, Y. M. Shin, M. K. Kim, C. H. Kim and J. S. Choi, Physicochemical properties of Sancho (Zanthoxylum schinifolium) seeds oil base extracts from different method. Korean J. Food Preserv., 20(6), 827 (2013).   DOI
5 T. J. Kim, Korean resources plants. Publishing center of Seoul National University, Seoul, Korea, 266 (1998)
6 S. M. Bae, Y. M. Jin, E. H. Jeong, M. B. Kim, H. Y. Shin, C. W. Ro and S. C. Lee, Studies on proximate composition, fatty acids and volatile compounds of Zanthoxylum schinifolium fruit according to harvesting time. Korean J. Medicinal Crop Sci., 19(1), 1 (2011).   DOI
7 S. Kim, Y. Han, Isolation and identification of antimicrobial compound from Sancho (Zanthoxylum schinifolium). Korean J. Soc Food Sci., 13(1), 56 (1997).
8 S. K. Hur, S. S. Kim, Y. H. Heo, S. M. Ahn, B. G. Lee and S. K. Lee, Effect of the grapevine shoot extract on free radical scavenging activity and inhibition of pro-inflammatory mediator production in raw 264.7 macrophages. J. Applied Pharmacology. 9(3) : 188 (2001).
9 M. Roberforid, P. B. Calderon, Free radicals and oxidation phenomena in biological system. Marcel Dekker, Inc., New York. 193 (1995).
10 J. W. Lee, Volatile flavor components of Korean sancho fruit and tree (Zanthoxylum schinifolium). Korean J. Community Nutr., 11(5), 493 (1998).
11 Akira Murakami and Hajime Ohigashi, Targeting NOX, iNOS and COX-2 in inflammatory cells. Int. J. Cancer, 121(11), 2357 (2007).   DOI   ScienceOn
12 W. K. Alderton, C. E. Cooper and R. G. Knowles, Nitric oxide synthase : Structure, function and inhibition. Biochem J., 357, 593 (2001).   DOI   ScienceOn
13 H. S. Ryu, K. H. Chang, H. W. Yang, M. S. Kim, H. C. Kwon and K. S. Oh, High cyclooxygenase-2 expression in stage IB cervical cancer with lymph node metastasis or parametrial invasion. Korean J. Gynecol. Oncol., 76, 320 (2000).   DOI   ScienceOn
14 D. K. Gaffney, J. Holden, M. Davis, K. Zempolich, K. J. Murphy and M. Dodson, Elevated cyclooxygenase-2 expression correlates with diminished survival in carcinoma of the cervix treated with radiotherapy. Int J. Radiat Oncol. Biol. Phys., 42(9), 494 (2001).
15 J. R. Vane, Y. S. Bakhle and R. M. Botting, Cyclooxygenases 1 and 2. Annu. Rev. Pharmacol. Toxicol., 38(1), 97 (1998).   DOI   ScienceOn
16 J. Hyuk Lee, K. M. Chang and G. H. Kim, Composition and anti-inflammatory activities of Zanthoxylum schinifolium essential oil:suppression of inducible nitric oxide synthase, cyclooxygenase-2, cytokines and cellular adhesion. J. Science of Food and Agriculture, 89(10), 1762 (2009).   DOI   ScienceOn