• Title/Summary/Keyword: 산업곤충

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Growth characteristics of mealworm Tenebrio molitor (갈색거저리의 발육특성)

  • Kim, Sun Young;Park, Jong Bin;Lee, Young Bo;Yoon, Hyung Joo;Lee, Kyeong Yong;Kim, Nam Jung
    • Journal of Sericultural and Entomological Science
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    • v.53 no.1
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    • pp.1-5
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    • 2015
  • As increasing utilization of Tenebrio molitor for industry, establishing effective conditions of indoor mass-rearing system become more important. For this reason, the aim of this study was to identify the physiological characteristics of Tenebrio molitor in different range of temperatures, photoperiods and rearing densities for mass breeding. As a result, their egg had above 70% hatching rate at $17.5{\sim}27.5^{\circ}C$ and the egg period was shown 5~7 days in $25{\sim}35^{\circ}C$. Consequently, in the consideration of the hatching rates and egg periods, the optimal indoor temperature for rearing was $25{\sim}27.5^{\circ}C$. Furthermore, development period of larvae in mass breeding system was shorter than individual breeding system under all the conditions of temperature. Also, the pupal weight was higher in mass breeding system except for $20^{\circ}C$ and $25^{\circ}C$. Considering elasticity of demand and weight of production, the effective condition was mass-breeding system at $25^{\circ}C$. In photoperiodic condition, the shortest of larval period was 157.35 day in 14L : 10D.

Expression of the blue fluorescent protein in fibroin H-chain of transgenic silkworm (피브로인 H-chain 재조합 단백질 발현시스템을 이용한 청색형광단백질의 발현)

  • Kim, Seong Wan;Yun, Eun Young;Choi, Kwang-Ho;Kim, Seong Ryul;Park, Seung Won;Kang, Seok Woo;Goo, Tae Won
    • Journal of Sericultural and Entomological Science
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    • v.52 no.1
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    • pp.25-32
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    • 2014
  • We produced the transgenic silkworm that expressed the enhanced blue fluorescent protein (EBFP) in the cocoon of silkworms. The EBFP fusion protein, each with N- and C-terminal sequences of the fibroin H-chain, was designed to be secreted into the lumen of the posterior silk glands. The expression of the EBFP/H-chain fusion gene was regulated by the fibroin H-chain promoter. The use of the $3{\times}P3$-driven DsRed2 cDNA as a marker allowed us to rapidly distinguish transgenic silkworm. A mixture of the donor and helper vector was micro-injected into 300 eggs of silkworms, Baegokjam. We obtained 5 broods. The cocoon displayed blue fluorescence, proving that the fusion protein was present in the cocoon. Also, the presence of fusion proteins in cocoons was demonstrated by SDS-PAGE and western blot analysis. Accordingly, we suggest that the EBFP fluorescence silk will enable the production of the silk-based biomaterials.

Production of fluorescent green silk using fibroin H-chain expression system (피브로인 H-chain 재조합 단백질 발현시스템을 이용한 녹색형광실크 생산)

  • Kim, Seong Wan;Yun, Eun Young;Choi, Kwang-Ho;Kim, Seong Ryul;Park, Seung Won;Kang, Seok Woo;Goo, Tae Won
    • Journal of Sericultural and Entomological Science
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    • v.51 no.2
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    • pp.153-158
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    • 2013
  • To express green fluorescent protein in the cocoon of silkworm, we constructed the fibroin H-chain expression system to produce enhanced green fluorescent protein (EGFP) in the cocoon of transgenic silkworms. The EGFP fusion protein, each with N- and C-terminal sequences of the fibroin H-chain, was designed to be secreted into the lumen of the posterior silk glands. The expression of the EGFP/H-chain fusion gene was regulated by the fibroin H-chain promoter. The use of the 3xP3-driven DsRed2 cDNA as a marker allowed us to rapidly distinguish transgenic silkworm. A mixture of the donor and helper vector was micro-injected into 1,200 eggs of bivoltin silkworms, Baegokjam. We obtained 8 broods. The cocoon displayed strong green fluorescence, proving that the fusion protein was present in the cocoon. Also, the presence of fusion proteins in cocoons was demonstrated by SDS-PAGE and immunoblotting. Accordingly, we suggest that the EGFP fluorescence silk will enable the production of the novel biomaterial based on the transgenic silk.

Development of nature friendly characters for infants and toddlers (영유아를 대상으로 한 자연친화적 캐릭터 개발 연구)

  • Joo, Eun-Ryeong;Chung, Jean-Hun
    • Journal of Digital Convergence
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    • v.16 no.3
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    • pp.415-422
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    • 2018
  • The purpose of this paper is to show development examples of nature-friendly characters and to play a guide role for various nature-friendly characters to appear by presenting nature-friendly characters setting in Korea. Three famous examples of nature-friendly characters in foreign countries and the influence of nature on infants and young children will be examined with reference to previously published papers and published books. I have confirmed that nature harmony positively affects emotions and life respect of infants and young children, the characteristics of nature-friendly characters are 1. The characters are drawn based on nature environment as background. 2. They have been beloved for story of nature conservation and love for nature, and playing in nature environment. As story setting, I suggested 1. A story of forest experience with friends 2. A story of nature observation and exploration such as playing, exploration, and explanation 3. A drawing of Korea nature environment, including grass, flower, tree, insect, etc. 4. An educational and philosophical story of life respect, nature conservation, and love for nature.

The Production, Distribution, and Utilization of Rice in Japan (일본의 쌀 생산, 유통 및 이용 현용)

  • Inouchi, Naoyoshi
    • Food preservation and processing industry
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    • v.1 no.1
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    • pp.17-26
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    • 2002
  • 일본은 기후적으로도 쌀의 생산에 적합하며 쌀은 일본의 주곡이다. 1893년 국립농업연구소가 설치된 이래로 쌀은 품질과 단보당 생산량이 꾸준히 개선되어와 116의 노동시간으로 생산량은 2.5배의 증가를 가져왔다. 이러한 진전은 재배방법의 개선, 비료, 농약, 재배기술, 기계화, 관개배수, 농지개량 등의 요인들로 볼 수 있다. 현재 일본에서 생산되고 있는 주요 쌀 품종으로는 코시히카리($35.5\%$), 히토메보레($9.7\%$), 히노히카리($9.0\%$), 아키다코마치($8.5\%$), 키라라($4.8\%$) 등 10여 품종이며 최근에는 형질전환 품종의 연구와 함께 생물공학적 연구 ,생리, 품질, 곤충과 잡초, 토양, 식품가공 등 기본적인 연구에 심혈을 기울이고 있다. 새로운 형태의 쌀 품종으로는 냉동조리쌀밥, 초밥, 레토르트룡 쌀 등 가공용으로 적합하도록 하기 위하여 아밀로오즈의 함량을 $5-15\%$로 낮춘 쌀이나 카레, 필라프, 튀김 쌀 등의 용도에 적합하도록 아밀로오스의 함량을 $30\%$이상으로 높인 쌀뿐만 아니라 곡립의 크기와 길이 등을 변형시킨 쌀, 유색미, 향미, 단백질 함량 조절 쌀, 거대 배아미, lipoxygenase 활성을 없앤 쌀, 단맛나는 쌀 등이 연구, 생산되고 있다. 일본에서의 쌀이용은 먼저 가공용 쌀을 들 수 있다. 밥, 청주, 스낵, 쌀가루, 미소발효 등 다양하게 이용이 되고 있으며 parboiled rice와 이의 색과 향을 개선한 converted rice, 현미를 이용한 가공쌀, 쌀빵, 현미가루, 세척미, 쌀국수, 쌀스낵, 당과, 죽, 쌀은 채소아 함께하는 타키코미고한, 초밥, 냉동쌀밥, 무균포장밥, 건조밥, 즉석밥 등 매우 다양하다.는 일, 쌀 재배구조 조정과 함께 높은 미질을 가지는 품종육종, 기계화를 비롯한 경작기술의 발달, 쌀과 부산물 가공기술의 개발연구, 특정기능을 함유하는 유전공학적 기술의 적용, 토지와 도시화 그리고 식량순환에 시스템의 개혁 등 과학기술을 고양하는 일 등을 들 수 있다.하는데 도움이 되리라 생각된다.=0.002)가 통계적으로 유의한 인자였다. 결론 : 본 기관에서 시행되어진 근침윤성 방광암에 대한 방광보존치료법은 기존의 근치적 방광절제술에 비하여 대등한 치료성적을 내는 동시에 $63\%$에서 장기보존이 가능하였다. 따라서 본 치료법이 방광암의 치료에 적극적으로 적용되어야 할 것으로 생각하며 향후 여러 기관이 참여하는 활발한 연구를 통해 한국인에게 가장 적절한 치료법을 개발해야 될 것으로 생각한다. B2+3, C1, C2+3군에서 수술달독군과 방사선치료 추가군의 골반종양제어율은 각각 $79\%$$75\%$ (p=0.88), $100\%$$100\%,\;44\%$$68\%$ (p=0.01)이었다. 전체 환자를 대상으로 다요인 분석을 시행한 결과 생존율과 무병생존율에 병기만이 유의하였고 두 치료 군에서도 역시 병기가 유의한 인자로 나타났다. 전체환자에서 골반종양제어율에 유의한 예후인자로 다요인분석을 시행한 결과 병기와 수술방법이 유의하였다. 수술단독군에서는 병기만이 유의하였고 방사선치료 추가군에서는 수술방법만이 유의하여 복부회음절제술군의 골반종양재발률이 높았다. 결론 : 본 후향적 연구에서 수술 후 보조적 방사선치료를 시행하여 수술단독군에 비해 병기 C2+3군에서 골반종양제어율이 향상되었음을 알 수 있었다. 그러나 병기 B2이상의 모든 환자에서 골반종양제어율 뿐만 아니라 생존율의 향상을 가져오기

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Economic Value Evaluation of Ecosystem Services in Organic Rice-Fish Mixed Farming System in Paddy Wetland (벼-담수어 유기농 복합생태 논습지의 생태계서비스 경제적 가치평가)

  • Nam, Hongshik;Byeon, Youngwoong;Park, Keechoon;Park, Kwanglai;Lee, Youngmi;Han, Eunjung;Kim, Changhyun;Kong, Minjae;Son, Jinkwan
    • Journal of Wetlands Research
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    • v.22 no.4
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    • pp.286-294
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    • 2020
  • This study aims to economically evaluate rice production and various ecological services provided by organic rice paddy carrying out rice-fish mixed farming. It was also conducted to find a stable structure for increasing the income of rice-producing farmers and promoting ecological services by evaluating the economic feasibility of organic rice paddy for rice-fish mixed farming. As a result of the analysis of expected effects by ecological service functions according to the types of agriculture in the rice-fish mixed farming, general conventional paddy fields were evaluated to have an effect of continuously decreasing biodiversity such as amphibian reptiles, aquatic insects, bird habitats, experience and ecological education, and vegetation diversity, while the biodiversity of organic and rice-fish mixed paddy fields was assessed to increase. As a result of evaluating the economic value, as the area for fish production increases compared to the common practice and organic paddy fields, rice production decreases, but fish production increases and total income was analyzed to increase. In addition, if the value of ecosystem service functions is assessed in the future, it will increase further. It is hoped that these findings will be used as basic data for solving oversupply problems in the rice industry, preserving safe farm income, and improving sustainable farming and ecosystem service functions.

Protective Effects of Water/Methanol Extracts of Cricket on the Acute Hepatic Damages in the ICR-mice Induced by Administration of $CCl_4$ (귀뚜라미의 수용성 및 알콜 추출물의 간보호 효과)

  • Ahn, Mi-Young;Lee, Yong-Woo;Ryu, Kang-Sun;Lee, Heui-Sam;Kim, Ik-Soo;Kim, Jin-Won;Lee, Yong-Ki;Kim, Eun-Sun;Kim, Yeong-Shik
    • Korean Journal of Food Science and Technology
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    • v.34 no.4
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    • pp.684-687
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    • 2002
  • Water and methanol extracts of cricket were examined for their liver protective effects against $CCl_4-intoxication$ in ICR-mice. Serum transaminases (S-GOT and S-GPT), lactate dehydrogense (LDH) and glutathione-S-transferase (GST) activities and TBARS (Thiobarbiturate-reactive substances) content were measured for evaluation of liver protective effects. The activities of GOT, GPT, LDH and hepatic content of lipid peroxide after $CCl_4-treatment$ were higher than normal control but those levels decreased th 74, 50, 101 and 40%, respectively, by the treatment of cricket methanol extract. The anti-fatigue effects of water and methanol extracts investigated by an acute weight-loaded forced swimming test showed significantly prolonged swimming time in the mice administered cricket extracts. These results suggest us that water/alcohol extract of G. bimaculatus may be used as a liver protective food.

Construction of fluorescent red silk using fibroin H-chain expression system (누에 형질전환에 의한 견사선에서의 적색형광단백질 발현)

  • Kim, Sung Wan;Yun, Eun Young;Choi, Kwang-Ho;Kim, Seong Ryul;Park, Seung Won;Kang, Seok Woo;Kwon, O-Yu;Goo, Tae Won
    • Journal of Sericultural and Entomological Science
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    • v.50 no.2
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    • pp.87-92
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    • 2012
  • We constructed the fibroin H-chain expression system to produce Discosoma sp. red fluorescent protein variant2 (DsRed2) in transgenic silkworm cocoon. Fluorescent cocoon could be made by fusing DsRed2 cDNA to the heavy chain gene and injecting it into a silkworm. The DsRed2 fusion protein, each with N- and C-terminal sequences of the fibroin H-chain, was designed to be secreted into the lumen of the posterior silk glands. The expression of the DsRed2/H-chain fusion gene was regulated by the fibroin H-chain promoter. The use of the 3xP3-driven EGFP cDNA as a marker allowed us to rapidly distinguish transgenic silkworms. The EGFP fluorescence became visible in the ocelli and in the central and peripheral nervous system on the seventh day of embryonic development. A mixture of the donor and helper vector was micro-injected into 1,020 Kumokjam, bivoltin silkworm eggs. We obtained 6 broods. The cocoon was displayed strong red fluorescence, proving that the fusion protein was present in the cocoon. Accordingly, we suggest that the DsRed2 fluorescence silk will enable the production of novel biomaterial based on the transgenic silk.

Development of Ultra-Rapid Reverse-Transcription PCR for the Rapid Detection against Slow Bee Paralysis Virus (SBPV) (Slow Bee Paralysis Virus (SBPV) 신속 검출을 위한 초고속 역전사 중합효소 연쇄반응법의 개발)

  • Kim, Somin;Lim, Sujin;Kim, Jungmin;Lim, Yoon-Kyu;Yoon, Byoungsu
    • Journal of Apiculture
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    • v.32 no.3
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    • pp.171-180
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    • 2017
  • Slow Bee Paralysis Virus (SBPV) is a pathogenic virus against honeybee and bumblebee, causes the death of adult bee by paralyzing the fore-leg of bee. In this study, for rapid detection of SBPV from bumblebee, SBPV-specific Ultra-rapid Reverse transcription PCR was developed. After optimizing of SBPV-specific Ultra-rapid PCR, the existence of $1.0{\times}10^8$ SBPV-specific DNA molecules could be recognized in 3 minute and 35 seconds. Even $1.0{\times}10^1$ molecules of SBPV-specific DNA could be measured with quantitative manner. Meanwhile, from both imported bumblebee and bumblebee produced in Korea, SBPV were detected using proposed method. In the laboratory as well as in the field, SBPV-specific Ultra-rapid Reverse transcription PCR would be applied and might be expected as useful tools at production of bumblebee or inspection for the import and export system of bumblebee.

Salt Removal and Agricultural Application of Food Waste-Biochar (음식폐기물바이오차의 염분 제거 및 농업적 활용)

  • Sin-Sil Kim;Jun-Suk Rho;Jae-Hoon Lee;Ah-Young Choi;Seul-Rin Lee;Yu-Jin Park;Jong-Hwan Park;Young-Han Lee;Dong-Cheol Seo
    • Korean Journal of Environmental Agriculture
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    • v.42 no.2
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    • pp.159-167
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    • 2023
  • Food waste (FW) emissions in South Korea amounted to 4.77 million tons in 2021, and continue to increase. Various technologies have been developed to treat FW, with recent research focusing on biochar production through pyrolysis to reduce FW. However, the agricultural application of food waste-biochar (FWBC) is limited by the salt accumulated during pyrolysis. This study investigated salt removal from and the kinetic characteristics of FWBC, and subsequently evaluated its agricultural applications. FW was pyrolyzed at 350℃ for 4 h, and subsequently washed for 0.1, 0.25, 0.5, 0.75, 1, 5, 15, and 30 min to remove salt. FWBC had a salt concentration of 5.75%, which was effectively removed through washing. The salt concentration decreased rapidly at the beginning (1 min) and then slowly decreased, unlike in FW, in which the salt decreased continuously and slowly. The salt removal speed constant (K) was 1.5586 (Stage 1, FWBC) > 0.0445 (Stage 2, FWBC) > 0.0026 (FW). In a lettuce cultivation experiment, higher biomass was achieved using washed FWBC than when using unwashed FWBC and FW, and soil properties were improved. Overall, these findings suggest that although FW reduction using pyrolysis causes a salt accumulation problem, the salt can be effectively removed through washing. The use of washed FWBC can enhance plant growth and soil properties.