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http://dx.doi.org/10.7852/jses.2014.52.1.25

Expression of the blue fluorescent protein in fibroin H-chain of transgenic silkworm  

Kim, Seong Wan (National Academy of Agricultural Science, RDA)
Yun, Eun Young (National Academy of Agricultural Science, RDA)
Choi, Kwang-Ho (National Academy of Agricultural Science, RDA)
Kim, Seong Ryul (National Academy of Agricultural Science, RDA)
Park, Seung Won (Department of Biotechnology, Catholic University of Daegu)
Kang, Seok Woo (National Academy of Agricultural Science, RDA)
Goo, Tae Won (National Academy of Agricultural Science, RDA)
Publication Information
Journal of Sericultural and Entomological Science / v.52, no.1, 2014 , pp. 25-32 More about this Journal
Abstract
We produced the transgenic silkworm that expressed the enhanced blue fluorescent protein (EBFP) in the cocoon of silkworms. The EBFP fusion protein, each with N- and C-terminal sequences of the fibroin H-chain, was designed to be secreted into the lumen of the posterior silk glands. The expression of the EBFP/H-chain fusion gene was regulated by the fibroin H-chain promoter. The use of the $3{\times}P3$-driven DsRed2 cDNA as a marker allowed us to rapidly distinguish transgenic silkworm. A mixture of the donor and helper vector was micro-injected into 300 eggs of silkworms, Baegokjam. We obtained 5 broods. The cocoon displayed blue fluorescence, proving that the fusion protein was present in the cocoon. Also, the presence of fusion proteins in cocoons was demonstrated by SDS-PAGE and western blot analysis. Accordingly, we suggest that the EBFP fluorescence silk will enable the production of the silk-based biomaterials.
Keywords
Fluorescent; Transgenic silkworm; EBFP; Bombyx mori; Silk;
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