• Journal of Veterinary Clinics
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• v.11 no.1
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• pp.471-478
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• 1994

The hematologic values and serum chemistry were examined healthy male 12 Pere David's Deer and 8 Wapiti at the Farmland Zoological Garden. In wapiti, the blood values collected from horn and jugular vein were compared. The results were as follows: 1. There were not any significant differences in hematologic values and serum chemistry of blood col. looted from horn and jugular vein in wapiti. 2. In Pere David's Deer, Mean$\pm$SD Values of White Blood Cell(WBC) count were 4673.50$\pm$1893.42/${mu}ell$, of Neutrophils 65.42$\pm$12.7%, of Lymphocytes 31.50$\pm$8.68%, of Monocytes 0.25$\pm$0.43%, of Eosinophils 0.17$\pm$0.37%. of Basophils, of Red Blood Cell(RBC) 9.93$\pm$1.38$\times$10/${mu}ell$, of PCV 54.25$\pm$6.25%, of Hb 16.89$\pm$2.43g/㎗, of Tot protein 7.63$\pm$0.6g/㎗, of Cholesterol 87.25$\pm$10.76mg/㎗, of Glucose 109.36$\pm$43.7mg/㎗, of SGOT 85.17$\pm$33IU/$\ell$, of SGPT 102.92$\pm$31.49IU/$\ell$, of BUN 21.00$\pm$5.18mg/㎗, of Creatinine 2.1$\pm$0.43mg/㎗, of ALKP 156$\pm$32.72IU/$\ell$, of Ca 11.28$\pm$1.04mg/㎗, of Na 127.92$\pm$10.04mmo1/$\ell$, of NH3 102.83$\pm$42.25mmo1/$\ell$, of Cl 105.91$\pm$3.45mmo1/$\ell$, respectively. 3. In wapiti, Mean$\pm$SD Values of White Blood Cell(WBC) count were 3450.00$\pm$1040.73/${mu}ell$, of Neutrophils 53.88$\pm$7.42%, of Lymphocytes 36.00$\pm$5.72%, of Monocytes 1.13$\pm$1.05%, of Eosinophils 1.38$\pm$1.93%, of Basophils 7.63$\pm$4.15%, of Red Blood Cell 10.09$\pm$1.3$\times$10/${mu}ell$, of PCV 46.88$\pm$3.33%, of H 15.09$\pm$1.48g/㎗, of Total protein 7.15$\pm$0.73g/㎗, of Cholesterol 78.63$\pm$12.27mg/㎗, of Glucose 75.63$\pm$33.22mg/㎗ , of SGOT 79.25$\pm$18.43IU/$\ell$, of SGPT 36.50$\pm$19.20IU/$\ell$, of BUN 29.63$\pm$4.15mg/㎗, of Creatinine 1.35$\pm$0.21mg/㎗ , of ALKP 283.50$\pm$88.13IU/$\ell$, of Ca 9.78$\pm$0.43mg/㎗ , of Na 120.00$\pm$10.02mmol/$\ell$, of NH3 261.50$\pm$ 103.46mmol/$\ell$, of Cl 92.00$\pm$9.19mmol/$\ell$, respectively.

• Food Science of Animal Resources
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• v.27 no.2
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• pp.209-215
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• 2007

In order to develop a sensitive and reliable method for the species-specific molecular markers, PCR-RFLP assay of the mitochondrial DNA(mt DNA) 12S rRNA gene was exploited for the identification of the origin of animal meat species including cattle, pig, sheep, goat, horse, deer, chicken, duck and turkey. A specific primer pairs were designed, based on the nucleotide sequences of mt 12S rRNA gene, for the amplification of the highly conserved region in the gene of the animal species using PCR-RFLP technique. mt DNA was isolated from meat samples followed by DNA amplification using PCR with the specific primers. PCR amplification produced an approximately 455 bp fragment in each of these animal meats. To distinguish pleat species, the PCR amplicons were digested with restriction endonucleases Tsp5091 and MboI, respectively, which generates distinct RFLP profiles. The DNA profiles digested with Tsp5091 allowed the clear discrimination in the mammalian meat species and the DNA profiles digested with MboI in poultry meat species. Therefore, the PCR-RFLP profiles of mt 12S rRNA gene could be very useful to identify the origin of the raw materials in the raw meats as well as the processed meat products.

• Food Science of Animal Resources
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• v.23 no.4
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• pp.309-314
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• 2003

Color and its stability in venison, longissumus dorsi (LD) and quadriceps femoris (QF) muscles, from 8 wild Cervus nippon yesoensis (Japanese Yeso Deer) were investigated by means of the CIE L$^{*}a{*}b{*}$ measurement and autoxidation rate recorded using partially purified myoglobin. It was observed a common feature of the change of three mean values($L^{*}$, $a^{*}$ and $b^{*}$) in both LD and QF that mean value increased at 1 or 2 day post-mortem and then decreased during storage. The differences between 1 and 7 days was the largest in $a^{*}$ value than those in $L^{*}$ and $b^{*}$ values. The mean differences among storage days were only significant in $a^{*}$ except for $b^{*}$ of LD. It was same tendency that the mean difference of CIE $L^{*}$, $a^{*}$ and $b^{*}$ values during refrigerator storage was larger in $a^{*}$ than both in $L^{*}$ and $b^{*}$ reported in beef(Sekikawa et al., 1995) and venison(Stevenson et al., 1989) during storage. The smaller $a^{*}$ value was indicated that bright red of meat changed to dull red, brown red causing met-Mb formation. To compare of color stability with respects to the Mb autoxidation rate, we measured this rate of deer and horse muscles, because horse Mb was considered to have the fastest autoxidation rate among domestic animals, and we used crude Mb and pH 6.0, which might be reflected to the intact meat. Mean value of the autoxidation rate measured in this study in deer was 0.037 and that was 0.026 in horse(sigma). Although there was no significant mean difference and were different Mb purity between deer(A409/A 280 nm = 4.0) and horse(5.6), in generally Mb purity was the higher and the faster autoxidation rate, but this rate in deer was faster than in horse. These results might indicate that venison meat discolors at faster rate compared with beef.

• Journal of Veterinary Clinics
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• v.26 no.6
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• pp.595-599
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• 2009

Sarcocystis grueneri-like sarcocysts were found from the cardiac muscles of a rearing red deer (Cervus elaphus) carcass in Korea. In the light microscopical examination of sarcocysts, they were oval to spherical cysts and 90-170$\times$110-380 ${\mu}m$ in size. However, there was no inflammation and myofiber degeneration. In the transmission electron microscope, these cysts were located within the sarcoplasm of the host cell and filled with merozoites. The sarcocysts were enclosed by a very thin wall (0.45-0.6 ${\mu}m$ thick) that consists of protrusions and ground substance. The primary cyst wall formed numerous strip-like protrusions which were 0.2-0.3 ${\mu}m$ wide and up to 4.2 ${\mu}m$ long. The protrusions were running in parallel with the surface of the cyst. A characteristic of the cyst wall was absent of fibrils inside the protrusions. Merozoites in the compartment measured about $15\times4\;{\mu}m$. The merozoite consisted of four regions: micronemes and rhoptries, amylopectin granule, nucleus, and amylopectin granules. The number of rhoptry was counted in 7-13.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.33 no.3
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• pp.58-65
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• 2015

Compared to pavement of Chinese palace gardens, those of private gardens in south part of the Yangtze River(長江) in China shows variety of patterns. In order to figure out what causes the difference in paving pattern, this article focuses on the traditional Chinese ideologies during the all time. An analysis on 'Classical Gardens of Suzhou', which has been designated as UNESCO World Heritage, was used to determine how ideologies have influence on paving pattern of Classical Gardens. As a result, various Chinese ideologies such as Nature worship, Totemism, Confucianism, Taoism, Buddhism, and Folk Culture were found in paving patterns of the private gardens. Pattern of sun in the Retreat & Reflection Garden(Toisawon) is an example of Nature worship among primitive beliefs of ancient China. Phoenix pattern of the Couple's Retreat Garden(Ouyuan, Liuyuan) reflects Totemistic beliefs. Confucianism is the underlying philosophy of Eight Square pattern, Six Square pattern, and Cross Square pattern. These patterns were mainly used to pave large area and easily found in many gardens. Patterns reflect Taoism are "Wufu holding life" pattern(the Five blessing holding life, 五福捧壽), Flat peach pattern, and Alluding Eight Immortals pattern. Paving patterns related to Buddhism are patterns of 'Eight treasures' and Endless knot pattern appears most frequently since it expresses concept of reincarnation well. Paving patterns shows folk culture and beliefs of the time in China are bat, butterfly, dragonfly, frog, carp, and coin(ingot).

• Journal of the Korean Geographical Society
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• v.39 no.2
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• pp.229-240
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• 2004

This article aimed to suggest a program for environmental protection and sustainable development in southwest Primorskii Krai of the Russian federation by analysis of Natural conditions, natural resources and current economic activities. Issues relating to protecting unique biodiversity toward economic development are examined. Special attention is given to functional zoning of the Russian part of lower Tumen River drainage. Functional zoning is key to protecting the unique biodiversity found in wet and marshlands of the lower Tumen River. Recommendations for acceptable economic activity are provided. The major components for a Sustainable Development Program in this area are: to define economically effective and ecologically acceptable types of regional activity (aquaculture; deer farming; recreation) and effective forms of territorial organization; to determine the restrictions on regional industrial and economic development; to organize rational regional land use by carrying out functional zoning and by coordinating this activity with all land users, including restructuring existing protected territories into a single system and include it into an international biospheric network.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.11
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• pp.5063-5070
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• 2011

Foot-and-mouth disease occurs in cattle or wild animal with two hooves such as cow, pig, sheep, goat, and deer. In this study, survey on knowledge, awareness, and lifestyle about foot-and-mouth disease was performed on 398 elementary school in 5 regions in Gyeonggi-do. 49 people (92.5%) from the group who have been educated about the foot-and-mouth disease replied that they have heard of foot-and-mouth disease and 139 people (68.5%) from the group who have not been educated about the disease replied that they have not heard of the disease. About the knowledge regarding "Foot-and-mouth disease virus is destroyed at temperatures above $50^{\circ}C$, so cooked food is free from the virus", only 26 people (49.1%) from the educated group and 82 people (40.4%) have correct knowledge. When asked about when education on disease such as foot-and-mouth disease, both groups replied that the education should be done in elementary school. The FMD includes in Korea and is a disease where the infection occurrence is happening continuously from the various nation. Must prepare with a recognition and the knowledge where the people are proper.

• Proceedings of the Korea Water Resources Association Conference
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• 2016.05a
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• pp.569-569
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• 2016

본 연구에서는 소유역 내 오염원 현황에 따른 발생부하와 배출부하를 분석하고자 하였다. 대상유역은 경상북도 영천시 북안천 일대이며, 소유역을 6개의 세유역으로 구분하여 발생부하와 배출부하를 산정하였다. 오염원 현황자료는 2013년 전국오염원 조사자료를 기초로 하였으며, 행정구역 단위의 오염원 조사 및 행정구역과 유역의 경계가 일치 하지 않는 경우의 오염원별 점유율은 토지종합정보망(Land Management Information System, LMIS)의 연속지적도 자료를 이용하여 면적비로 산정하였다. 대상유역의 전체 인구는 총 5,140명이며, 이 중 시가 인구가 2명, 비시가 인구가 5,138명으로 나타났다. 생활계 오염원은 소유역 별 인구를 분석한 결과, FW-'1은 3,113명, FW-'2는 298 명, FW-'3은 604 명, FW-'4는 836 명, FW-'5는 283 명, 그리고 FW-'6은 6 명으로 분석되었다. FW-'1의 인구는 총 3,113명으로 대상유역에서 가장 많은 인구가 분포하고 있는 것으로 나타났다. 축산계 오염원은 FW-'1 지점에 총 115 가구의 농가가 위치하여 소유역 중 가장 많은 축산 농가가 위치하고 있으며. 가축 사육 두수는 돼지 8,040두, 젖소 394두, 한우 1,587두, 사슴 10두 그리고 가금(기타) 수가 292,158두로 다른 지점들과 비교하여 사육 두수가 가장 많은 것으로 나타났다. FW-'2 지점은 돼지 1,350두가 있는 돼지 농가가 1가구 위치하고 있는 것으로 나타났다. FW-'3 지점은 돼지 14,692두, 한우 1,287두, 가금(기타) 100두가 있으며, 축산 농가는 총 42가구가 위치하고 있는 것으로 나타났다. 다른 지점들과 비교하여 돼지 사육 두수가 가장 많은 것으로 나타났다. FW-'4 지점의 가축현황은 돼지가 900마리, 한우 1,222 마리이며, 총 57개의 농가가 위치하고 있고, 이 중 돼지 농가는 1가구이며, 그 외 56개의 농가는 모두 한우 농가이다. FW-'5 지점은 한우 407마리 가금(기타) 150마리가 있는 것으로 나타났으며, FW-'6 지점은 축산 농가가 존재하지 않는다. 또한 대상유역 내에는 총 14개의 산업체가 있으며, $800.33m^3/day$만큼의 폐수를 발생시키고 있다. 이중 FW-'1 지점 산업체 개수는 12개이며, 폐수 발생량은 $730.59m^3/day$로 가장 많은 양의 폐수가 발생하고 있다. 대상유역 내에는 산업계, 양식계, 매립계의 오염원은 없는 것으로 나타났다. 오염원 자료를 바탕으로 발생부하량과 배출부하량 산정결과 생활계와 축산계, 토지계 중축산계의 BOD, T-N, T-P 모두 가장 높은 것으로 분석되었다. 배출부하량 비교 결과에서도 BOD, T-N, T-P 모두 축산계 배출부하량의 비율이 높게 나타났으며, 이 중 BOD는 토지계 배출부하량이 차지하는 비율이 축산계 배출부하량보다 약간 높게 나타났다. T-N과 T-P는 축산계 배출부하량 비율이 가장 높은 것으로 분석되었다. 그러나 오염원의 정량적인 발생원 규명을 위해서는 오염원 그룹이 포함되어있는 지역에 대하여 장기적인 실측 모니터링을 통해 얻은 오염부하를 산정하여 비교?분석하는 연구가 필요할 것으로 판단된다.

• Proceedings of the Korea Water Resources Association Conference
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• 2016.05a
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• pp.574-574
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• 2016

유역으로 유입되는 오염물질의 발생원은 점오염원과 비점오염원으로 구분할 수 있으며 점오염원의 경우 생활하수, 산업폐수, 그리고 축산폐수에 대해 처리시설 확충 및 기술개발을 통해 관리하고 있다. 비점오염원에서의 오염물질 유출은 토지이용, 강우, 불특정적인 오염물질 투입상태 등에 따라 다르며, 지역적 특성에 영향을 받기 때문에 불확실성이 고려되어야 한다. 특히 농촌지역에서의 비점오염은 접근이 어렵고 관리주체가 모호하여 좀처럼 규명되지 않았으며, 전국적으로 그 영향이 정량화되지 않아 실질적인 관리 및 대책마련에 어려움이 있었다. 특히, 가축분뇨의 발생으로부터 처리, 자원화에 이르기까지 각 관리체계에 있어서 축산비점오염의 배출경로와 수계오염부하량, 수질환경 영향을 정밀하게 분석하여 향후 대책마련을 위한 기초자료를 확보할 필요가 있다. 또한 전국적으로 비점오염이 수계에 미치는 영향과, 그 중 축산비점오염의 영향을 면밀히 분석하여 향후 정책 및 제도개선을 위한 과학적 기초자료로서 활용할 필요가 있다. 따라서 본 연구에서는 축산 밀집 지역을 대상으로 오염원 조사를 통해 강우시 비점오염 모니터링 지점을 선정 하였으며, 년5회씩 강우 모니터링을 하여 기초데이터를 축적 하였다. 대상지역은 강원도 횡성군에 위치한 일리천 유역이며 농가 수는 총 90개의 농가가 위치하고 있는데 그 중 돼지 1,467마리, 한우 1,957마리, 젖소 581마리, 개 2,880마리, 닭 75,000마리, 사슴 4마리로 조사되었다. 대상유역을 대상으로 배출부하량을 조사한 결과 BOD 배출부하량은 총 509.3 kg/day, T-N 배출부하량은 총 331.5 kg/day, T-P 배출부하량은 총 28.3 kg/day로 조사되었다. 유출특성을 파악하기 위하여 유량가중평균농도(Event Mean Concentration, EMC)를 산정한 결과 BOD의 경우 MW-4에서 1.2 mg/L - 7.2 mg/L, MW-5에서 0.8 mg/L - 6.3 mg/L, MW-7에서 0.7 mg/L - 5.2 mg/L의 범위를 보였다. T-N의 경우 MW-4에서 1.426 mg/L - 5.321 mg/L, MW-5에서 1.205 mg/L - 4.27 mg/L, MW-7에서 0.989 mg/L - 3.859 mg/L의 범위를 보였다. T-P의 경우 MW-4에서 0.245 mg/L - 0.632 mg/L, MW-5에서 0.236 mg/L - 0.596 mg/L, MW-7에서 0.213 mg/L - 0.521 mg/L의 범위를 보였다. 본 연구에서 EMC를 산정한 방법은 평시 수질 및 유량을 정하는 기준에 따라 값이 많이 달라질 수 있다. 따라서 합리적으로 평시 부하량을 제외하고 강우시의 영향을 파악할 수 있는 EMC 산정방법에 대한 추가적인 고찰이 필요할 것으로 사료된다.

• Korean Journal of Soil Science and Fertilizer
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• v.31 no.2
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• pp.162-169
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• 1998

This study was carried out to assess the roles of soil and vegetation on the nutrient cycling in paddy ecosystem where excessive amounts of animal wastes were flowed in due to the rain. Experimental sites included one abandoned and four cropping paddy fields which were moderately terraced under a small farm village raising 90 milk cows and 35 deer under open-air condition. The watershed covered 4 ha with every 50% of uptown and fodder crops. Concentrations of $NH_4-N$ and $P_2O_5$ in waste water flowed into the abandoned paddy field, enforced by the rain of $56.4mm\;day^{-1}$, were $8.3mg\;{\ell}^{-1}$ and $1.8mg\;{\ell}^{-1}$, respectively. Total mass of rainfall inflow to abandoned field during rice growing period (1 May to 30 Sept.) was $20,900Mg\;ha^{-1}$. Total amounts of $NH_4-N$ and $P_2O_5$ contained in that inflow were estimated as 173 kg and 38 kg, respectively. Concentrations in the outflow water through one abandoned and four rice paddy fields were reduced by 92% for $NH_4-N$ and 95% for $P_2O_5$, as compared to those in the inflows. The reserved portions of nutrients in the abandoned paddy field ecosystem, which were the summation of the uptake by weed and residues in soil, were 29% of the inflow amount for $NH_4-N$ and 30% for $P_2O_5$. These results demonstrated that soil and vegetation in paddy field ecosystem reduced the excessive nutrients from the animal waste inflow to the extents that might be suitable not only for the better growth of rice plant, located at the lower paddy fields, but also for preservation of the downstream from eutrophication.