• Journal of the Korean Society of Food Science and Nutrition
• /
• v.22 no.1
• /
• pp.68-72
• /
• 1993

The methanol extract of the leaves of Cudrania tricuspidata and ethyl acetate fraction from the methanol extract showed inhibition for trypsin activity and the growth inhibition of Staphylococcus aureus. The content of kaempferol 7-O-$\beta$-D-glucopyranoside isolated from this plant was determined by HPLC and it was about 0.31% for the methanol extract.

• Journal of Bio-Environment Control
• /
• v.17 no.1
• /
• pp.20-25
• /
• 2008

This study was conducted to develop the industrial producing technology of sprout vegetables using the cut twigs of woody plants. We have cut the twigs of wild mulberry tree on 13 April,2006 to examine the sprouting rate as affected by the storage conditions on relative humidity of $40{\sim}50%$ and $80{\sim}90%$ under $4{\sim}6^{\circ}C$ temperature. And we have also investigated the sprouting growth following those storage period of twigs cut with $1{\sim}3$ buds. The longer storage periods, the less survival rate of wild mulberry twigs cut. The sprouting rate of cut twigs gathered on the day which had not stored at low temperature was 97.4%. And The sprouting rate at the relative humidity of $40{\sim}50%$ after storing at low temperature for 122 days were 34.2%, and that of at $80{\sim}90%$ relative humidity was 85.7%. The longer storage periods, the more the number, length and width of sprouts. Therefore, the twigs of a wild mulberry trees cut with $1{\sim}3$ buds were able to produce the sprout vegetables under the condition of temperature $4{\sim}6^{\circ}C$ and relative humidity $80{\sim}90%$.

• Journal of Sericultural and Entomological Science
• /
• v.52 no.2
• /
• pp.129-133
• /
• 2014

We analyzed rutin content using mulberry genetic resources. They were grown under the same environment and conditions. Mulberry leaves were collected and then freeze-dried and powdered for rutin test. As a result, mean content of mulberry strains was $0.38{\pm}0.17%$, and the coefficient of variation (CV) was 45.7%. The variation was greatly severe. Among the tested strains, 'Jangloe' was showed the highest content of 0.97%, whereas 'Sungsu 3' was showed the lowest content of 0.01% respectively. Finally we selected rutin high-containing 6 strains. They are as follows. 'Jangloe', 'Pumbo 6', 'Youjin', 'Kaeryangjeonjeon', 'Baekhak', and 'Pumbo 32' which are more than twice of the overall average content.

• Current Research on Agriculture and Life Sciences
• /
• v.14
• /
• pp.85-100
• /
• 1996

The Studies were conducted to provide the state of mulberry cultivation and it's development in Banguet province (high land) of Philippines. Philippines initiated the sericulture industry with the technical asistance of Japan in 1974 and established mulberry field and sericultural facilities with technical asistance of Korea in 1990 and 1995. The required average of 100 to 150mm per month is not available during the dry season from December to March. Therefore mulching with grass which is available abundantly in the Philippines should be established to conserve moisture, to control soil erosion, surface ran-off and also to increase the humus content in the soil. In chemical properties of mulberry field, the pH value of soil is 4.7, organic matter 1.6%, and available phosphorus 6ppm. Therefore, all fields should do liming and be applied compost. To improve leaf yield for mulberry planted under partial shade area of pine trees, more pruning of pine tree should be done for good sunshining of mulberry, more liming and compost should be applied to improve acidic soil. To control the leaf roller, DDVP and KAFIL are able to be used. When spraying insecticides to control mulberry insect pests, care should be taken to consider the residual effects of chemicals on the leaf. Leaf should be fed to silkworms only after the leaves are free of any residual effects.

• The Korean Journal of Mycology
• /
• v.26 no.4 s.87
• /
• pp.583-588
• /
• 1998

A fibrinolytic enzyme has been isolated from the edible honey mushroom, Armillariella mellea and purified. The apparent molecular mass of purified enzyme was estimated to be 19800Da by SDS polyacryl amide gel electrophoresis and 19900Da by gel filtration, indicating that it was a monomer. The enzyme was optimal at pH 7, suggesting that the purified enzyme was a neutral proteinase. It shows the maximum fibrinolytic activity at $55^{\circ}C$, is completely inactivated above $65^{\circ}C$, and still indicates 40% of activity at $37^{\circ}C$. The fibrinolytic activity has been decreased by the addition of EDTA. Fifteen amino acid sequence was determined by protein sequencing techniques.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.41 no.11
• /
• pp.1493-1501
• /
• 2012

The antioxidant activities of volatile aroma extracts from Cudrania tricuspidata (Carr.) Bureau were examined using two antioxidant assays. Ten volatile aroma compounds identified in this plant were also tested for antioxidant activity. The volatile aroma extracts of stem and root from C. tricuspidata exhibited antioxidant activities with a clear dose response relationship in both aldehyde/carboxylic acid and lipid/malonaldehyde assays. Antioxidant activities of volatile aroma extracts from C. tricuspidata at $500{\mu}g/mL$ were $77.02{\pm}8.12%$ (stem) and $74.19{\pm}6.82%$ (root) in the aldehyde/carboxylic acid assay. Antioxidant activities of volatile aroma extracts from C. tricuspidata at $160{\mu}g/mL$ were $76.17{\pm}4.25%$ (stem) and $61.43{\pm}2.11%$ (root) in the lipid/malonaldehyde assay. Positively identified volatile aroma components in extracts of stem and root from C. tricuspidata were seven terpenes and terpenoides, 14 alkyl compounds, 11 nitrogen containing heterocyclic compounds, three oxygen containing heterocyclic compounds, 12 aromatic compounds, nine lactones, and seven miscellaneous compounds (possible contaminants). Among the positively identified compounds, eugenol, isoeugenol, and 2,4-bis (1,1-dimethylethyl)phenol exhibited antioxidant activities comparable to those of BHT and ${\alpha}$-tocopherol. Vanillin and 2-acetylpyrrole showed moderate activities in the lipid/malonaldehyde assay. These results suggest that consumption of antioxidant-rich beverages prepared from C. tricuspidata could have beneficial effects on human health by preventing diseases caused by oxidative damage.

• Journal of Sericultural and Entomological Science
• /
• v.37 no.2
• /
• pp.109-113
• /
• 1995

Effects of sulfur application on mulberry growth and soil chemical properties were examined in mulberry trees grown in a green house. Mulberry graftages were planted in Wagner pots and sulfur was applied at the levels of 0, 30, 60, or 120 kg/10a. Mulberry stem growth increased by 3.2 cm at 30 kg/ha than at 0 kg/ha sulfur application. Stem growth, however, decreased by 3.7 cm with the application of sulfur higher than at 60 kg/10a. Soil pH changed by the application of sulfur. With the application of sulfur at 120 kg/10a, soil pH decreased by 3.0. Temporal changes in the effective from sulfur content in soil indicated that sulfur dissolved between 4 to 10 days after application in the soil. Content of moisture and chemical components in mulberry leaves was also affected by the application levels of sulfur. Moisture, total-N, No3-N, K, and S content decreased, but F and Ca content increased with the application of sulfur lower than at 60 kg/10a. With the application of sulfur higher than at 60 kg/10a, P, S, and K content increased.

• Journal of Sericultural and Entomological Science
• /
• v.25 no.1
• /
• pp.27-33
• /
• 1983

The study was carried out to examine effect of cadmium spraying on mulberry leaves on the growth and metabolism of mulberry tree.The results obtained were as follow:1. Brown spot were appeared on the leaves as the symptoms of damage and the damage was remarkable with higher concentration of cadmium.2. Higher concentration of Cd inhibited the length and diameter of mulberry branches with a significant difference (P<0.05) for the length. The growth of branch length in both 5 and 7 ppm treatment of Cd was inhibited by 31% compared with the control. 3. Cadimum concentration of mulberry barks was noticeably lower than that of leaves. 4. Cadimum concentration over 3ppm inhibited significantly chlorophyll content, activitsies of respiration, glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transase (GPT) and adenocine triphosphate (APT). 5. The lowest concentration of cadmium was identified to be 3ppm to incur the inhibitory effect on the growth and metabolism of mulberry and cadmium content of mulberry leaves at the concentration was 13.86ppm.

• Journal of Sericultural and Entomological Science
• /
• v.30 no.2
• /
• pp.75-83
• /
• 1988

These experiments were carried out to define the rest period of mulberry by treating growth regulators in Taegu, Korea. Results obtained were as follows: It was recognized that the depth of rest in Taegu, Korea, was not deeper than that in Tokyo and Kagoshima, Japan. The rest of mulberry was begun at the end of September, subsequently became deeper through the first October into the late October and then turned gradually into quiescence by the beginning of November. Buds sprayed by gibberellic acid ($GA_3$) 10ppm and urea 0.5% were promoted to sprout, while naphthalene acetic acid (NAA) 0.02% inhibited strongly bud sprouting and abscisic acid (ABA) 20ppm had no effect on the rest of mulberry. Gibberellic acid 10ppm enhanced the rate of green color of bud after incubation for 10 days at $30^{\circ}C$. By the portion of mulberry stems, the depth of rest was different that the middle buds were less dormant than those lower. The optimal time required for the mulberry winter bud break is 15 days incubation at $30^{\circ}C$ as treated with $GA_3$.

• Journal of Sericultural and Entomological Science
• /
• v.53 no.2
• /
• pp.82-86
• /
• 2015

This study was carried out to develop the silkworm rearing technique in unused mulberry leaves after harvesting mulberry fruit. The growth of Gwasang No. 2 and Suhyang was very good compared to control cultivar Chungil in leaves size and new branch growth, but new branch and leaves of Daeshim was similar or small to control cultivar Chungil. The number of leaves of Gwasang No. 2 and Suhyang was lower than Chungil, but weight of leaves per tree was heavyer than Chungil. Mulberry leaf yield was Gwasang No. 2 521 kg/10a, Suhyang 189 kg/10a, Daeshim 73 kg/10a, Chungil 1,095 kg/10a. Content of mineral element of all three mulberry cultivars leaf for mulberry fruit production was higher than Chungil in N, P, K, Ca etc. Feeding quantity of silkworm of Gwasang No. 2 and Suhyang was much more than Chungil. Feeding quantity was highest at Suhyang in 96 kg/box. Growth duration of silkworm larvae was not different in all four mulberry culivars but weight of silkworms (5th instar 3rd day) was heavy at Gwasang No. 2 (2.07 g/head) and Suhyang (2.11 g/head) compared to control cultivar Chungil (1.92 g/head). Mortality of silkworms was 14.6% (Gwasang No. 2), 13.3% (Suhyang), 13.9% (Daesim), 12.6% (Chungil) and than higher at elder instar stage. The production amount of silkworm (5th instar 3rd day) was 35.4 kg/box (Gwasang No. 2), 36.6 kg/box (Suhyang), 35.0 kg/box (Daeshim), 33.6 kg/ box (Chungil). Amount of possible rearing silkworms was estimated 3.4box/10 a (Gwasang No. 2), 1.3box/10a (Suhyang), 0.5box/10a (Daeshim), 8.7box/10a (Chungil).