• Proceedings of the Membrane Society of Korea Conference
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• 2000.11a
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• pp.127-130
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• 2000

• Journal of Life Science
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• v.12 no.4
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• pp.392-398
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• 2002

38 strains were isolated in order to utilize lignin degrading ability from soil and compost. A organism having high lignin degrading ability of the isolated strains determined morphologcal and biochemical characteristics. Enrichment technique yielded a lignin degrading bacterium characterized as Pseudomonas sp. LC-2. This strain was able to degrade lignin which are the true representatives of native lignin and transform lignin to a lot of aromatic compounds as HPLC analysis of culture. By polyacrylamide gel analysis, it was determined that peroxidase consisted of three enzymes, with only one, the lignin peroxidase having high activity.

• KSBB Journal
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• v.16 no.5
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• pp.511-515
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• 2001

In this paper, we development and operated a two-stage continuous stirred tank reactor (CSTR)/trickling biofilter(TBF)system for the long-term continuous treatment of trichloroethylene (TCE) using Burkholderia cepacia G4. In this reactor system. CDTR with cell recycle from TBF was coupled to the TBF for the reactivation of the cells deactivated during TCE degradation. The critical elimination capacity was determined to be 25.3 mg TCE/L day and the reactor has been stably operated for more than 1 months, which clearly represented that CSTR/TBF system can be used for long-term treatment of TCE.

• Journal of Food Hygiene and Safety
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• v.19 no.2
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• pp.97-103
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• 2004

The present study was performed to investigate photodegradation rate constants and degradation products of piperophos by the USEPA method. The pesticide was very stable in 16 days exposure of sunlight from October 3 to 22, 2003 and humic acid had no sensitizing effect on the photolysis of each pesticide in sunlight. In the UV irradiation test, piperophos was rapidly degraded as increasing UV intensity. In case of UV irradiation with TiO$_2$ and with TiO$_2$ powder amount, degradation of piperophos was slower than UV irradiation. In order to identify photolysis product, the extracts of degradation product was analyzed by GC/MS. The mass spectrum of photolysis product of piperophos was at m/z 166. It was suggested that the photolysis products of piperophos was O, O-dipropyl phosphorodithioate.

• Journal of the Korean Chemical Society
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• v.40 no.3
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• pp.180-186
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• 1996

To determine burn-out condition of polyvinyl butyral as binder, TGA and GC/MS were used to analyze decomposed products of PVB and Ni green sheet during binder pyrolysis. The produced gases are similar in kind each other but the relative amount of produced gases was different. When the atmosphere air contains water, relatively larger amount of carboxylic acids and aromatic compounds were detected, and the amount of the decomposed products were larger in Ni green sheet than PVB alone. And Ni acted as a catalyst in the thermal decomposition of PVB.

• Korean Journal of Microbiology
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• v.55 no.1
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• pp.83-85
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• 2019

Pseudomonas sp. PAMC 29040 was isolated from a maritime tundra soil in Antarctica for its ability to degrade lignin and subsequently confirmed to be able to depolymerize heterogeneous humic substance (HS), a main component of soil organic matter. The draft genome sequences of PAMC 29040 were analyzed to discover the putative genes for depolymerization of polymeric HS (e.g., dye-decolorizing peroxidase) and catabolic degradation of HS-derived small aromatics (e.g., vanillate O-demethylase). The information on degradative genes will be used to finally propose the HS degradation pathway(s) of soil bacteria inhabiting cold environments.

• Korean Journal of Microbiology
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• v.55 no.2
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• pp.177-179
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• 2019

Pseudomonas kribbensis CHA-19 was isolated from a temperate forest soil (mid latitude) in New Jersey, USA, for its ability to degrade humic acids, a main component of humic substances (HS), and subsequently confirmed to be able to decolorize lignin (a surrogate for HS) and catabolize lignin-derived ferulic and vanillic acids. The draft genome sequence of CHA-19 was analyzed to discover the putative genes for depolymerization of polymeric HS (e.g., dye-decolorizing peroxidases and laccase-like multicopper oxidases) and catabolic degradation of HS-derived small aromatics (e.g., vanillate O-demethylase and biphenyl 2,3-dioxygenase). The genes for degradative activity were used to propose a HS degradation pathway of soil bacteria.

• Microbiology and Biotechnology Letters
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• v.40 no.2
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• pp.104-110
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• 2012

Glycosynthase is an active site nucleophile mutant enzyme, prepared from glycosidase, which is capable of synthesizing oligosaccharide derivatives without the hydrolysis of the product. Thermoacidophilic ${\alpha}$-glucosidase of Thermoplasma acidophilum (AglA) exhibits a transglycosylating activity yielding various glycosides. AglA was converted to glycosynthase by the substitution of the catalytic nucleophile Asp-408 residue into non-nucleophile glycine in order to increase its ability to synthesize various glycosides by transglycosylation. The glycosynthase mutant was purified by Ni-NTA chromatography and its glycoside-synthesizing activity was measured by using an external nucleophile, sodium formate buffer, providing maltose as a donor and p-nitrophenyl-${\alpha}$-D-glucopyranoside ($pNP{\alpha}G$) as an acceptor, respectively. In addition, $pNP{\alpha}G$ was examined for its feasibility to act as both a donor and an acceptor, and products were compared with those of the wildtype enzyme. The mutant enzyme was found to catalyze the formation of a specific product from $pNP{\alpha}G$ with a yield of 42.5% without further hydrolysis, while the wild-type enzyme produced two $pNP{\alpha}G$ products at low yields. The results demonstrate the possibility of satisfactory yields for the reactions in the presence of small amounts of acceptor, and demonstrate that the high activity of the mutant, at pHs below neutrality, was applicable in the transfer of glucose from the natural donor.

• Nuclear Engineering and Technology
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• v.13 no.4
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• pp.221-228
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• 1981

${\gamma}$-(U-$^{14}$ C)-BHC was applied to rice plants grown in a pot and its fate in the growth, polishing and oil-extraction processes of the grain was investigated. The $^{14}$ C-activity was absorbed and translocated widely in the plant and the recovery of applied $^{14}$ C-activity in the straw and grain was about 2.8%, of which 9.4% was found in the brown rice. The % partitioning of $^{14}$ C-residues in bran and polished rice was 12:88 and that in oil and oilcake was 37 : 63. Characterization of $^{14}$ C-residues indicated the presence of ${\gamma}$-BHC, pentachlorocyclohexene, trichlorobenzene and hydrophilic metabolites, whose proportions were different in the straw and grain.

• The Journal of Korean Medicine
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• v.23 no.4
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• pp.105-112
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• 2002

목적: 한의학에서 어혈증으로 야기되는 여러 가지 증상의 개선에 사용되는 실소산가미의 범발성혈관내응고증 및 혈전증에 미치는 영향을 연구하는 것이다. 방법 : 동물은 Wistar-King atrain Rats(150-200g)를 사용하였고, 혈전증은 세포내독소로 유발하였다. 측정은 실소산가미와 각 구성약물들에 대한 혈소판, 섬유소원, 프로트롬빈시간, 섬유소섬유소원분해산물에 미치는 영향을 연구하였다. 결과: 항혈전의 특성에 관한 것은 실소산가미와 구성약물중 포황, 오령지, 적작약, 도인 그리고 울금에서 억제특성이 나타났으며, 또한 실소산가미와 구성약물은 정상쥐에서 범발성혈관내응고증에서 혈소판과 섬유소원의 감소가 억제되었고, 섬유소분해산물의 증가가 억제 되었다. 실험관 실험에서 실소산가미와 구성약물은 트롬빈에 의해 섬유소원에서 섬유소로 전환이 억제되었으며, 플라스미노겐 또는 플라스민의 활성을 억제하였다. 결론: 실소산가미가 세포내독소로 유발된 혈전증에 대한 억제작용을 보이므로, 혈전증으로 야기되는 심혈관계질환등의 치료 및 예방에 응용가능성이 있을 것으로 사료된다.