• Title/Summary/Keyword: 분리.이주

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Studies on the Wave Propagation and Fluctuation in Randomly Ditribution Media of L-Aspartic Acid (L-Aspartic Acid의 무질서하게 분포된 매질에서 파동전개와 변동에 관한 연구)

  • Kim, Ki-Jun;Lee, Joo-Youb
    • Journal of the Korean Applied Science and Technology
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    • v.29 no.1
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    • pp.122-128
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    • 2012
  • The influences of fluorescence, scattering, and flocculation in turbid material by light scattering of L-aspartic acid, preservative and emulsifier were interpreted for the scattered fluorescence intensity and wavelength. They have been studied the molecular properties by the spectroscopy of laser induced fluorescence (LIF) and flocculation. The effects of optical properties in scattering media have been found by the optical parameters(${\mu}_s$, ${\mu}_a$, ${\mu}_t$). Flocculation is an important step in many solid-liquid separation processes and is widely used. When two particles approach each other, interactions of several colloid particles can come into play which may have major effect on the flocculation and LIF process. The values of scattering coefficient ${\mu}_s$ are large by means of the increasing scattering of scatterer, The values have been found that the slope decays exponentially as a function of concentration from laser source to detector by our experimental result. It may also aid in designing the best model for oil chemistry, bio-pharmaceutical products, laser medicine and application of medical engineering on LIF and coagulation in particle transport mode.

A Study on the Measurements of Beam Wave Propagation and Fluorescence Spectroscopy in Particles Media (입자매질에서 빔파동전파와 형광분광 측정에 관한 연구)

  • Kim, Ki-Jun;Lee, Jou-Youb;Sung, Wan-Mo
    • Journal of the Korean Applied Science and Technology
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    • v.31 no.2
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    • pp.167-175
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    • 2014
  • The influences of fluorescence, scattering, and flocculation in turbid material were interpreted for the scattered fluorescence intensity and wavelength, it has been studied the molecular properties by the spectroscopy of laser induced fluorescence(LIF). The effects of optical properties in scattering media have been found by the optical parameters(${\mu}_s$, ${\mu}_a$, ${\mu}_t$). Flocculation is an important step in many solid-liquid separation processes and is widely used in Photodynamic therapy. The interactions of several colloid particles can come into play which have major effect on the flocculation and LIF process. We measured scattering and fluorescence spectra of the sample for in vitro as function of concentration from lase source to detector. The value of scattering coefficient ${\mu}_s$ is large by means of the increasing particles of scatterer. Therefore, Phorphyrin A is larger than Phorphyrin C in scattering intensity ${\mu}_s$, but Phorphyrin A is smaller than Phorphyrin C in penetration depth ${\delta}$.

Measurement on the Methotrexate in L-${\alpha}$-Phosphatidylcholine Media by Light Sacttering (L-${\alpha}$-Phosphatidylcholine 매질에서 빔산란에 의한 Methotrexate의 측정)

  • Kim, Ki-Jun;Lee, Joo-Youb
    • Journal of the Korean Applied Science and Technology
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    • v.30 no.2
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    • pp.251-257
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    • 2013
  • The influences of Methotrexate as fluorophor, scatterer, absorber in turbid material by light scattering were interpreted for the scattered fluorescence intensity and wavelength, it has been studied the molecular properties by laser induced fluorescence spectroscopy. It has been found that the effects of optical properties in scattering media by the optical parameters((${\mu}_s$, ${\mu}_a$, ${\mu}_t$). The value of scattering coefficient ${\mu}_s$ is large by means of the increasing particles of L-${\alpha}$-Phosphatidylcholine, it has been found that the slope decays exponentially as a function of depth from laser source to detector. It may also aid in designing the best model for oil chemistry, laser medicine and application of medical engineering.

Quality Characteristics of Cereal Makgeolli Rice Nuruk Prepared Rhizopus oryzae CCS01 (Rhizopus oryzae CCS01로 제조된 쌀누룩을 이용한 곡류 막걸리의 품질 특성)

  • Cho, Hyeon-Kook;Seo, Weon-Taek;Lee, Ju-Young;Cho, Kye-Man
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.7
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    • pp.1002-1008
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    • 2012
  • For the production of Korea traditional cereal wine makgeolli, a rice fermentation starter nuruk was Rhizopus oryaze CCS01 commercial nuruk. The carbohydrate content of rice nuruk was higher, the levels of moisture, ash, crude protein, and crude fat were lower. In particular, the saccharifying activity of rice nuruk was 2.2 times higher than commercial nuruk. pH, alcohol, and viable yeast cells of makgeolli made rice nuruk were higher than those of makgeolli made commercial nuruk. In contrast, the levels of acidity, $^{\circ}Brix$, viable lactic acid bacteria (LAB) cells, and browning of makgeolli made rice nuruk were lower than those of makgeolli made commercial nuruk, the alcohol content of glutinous rice (GUR) makgeolli made rice nuruk higher other samples. These results suggest that rice nuruk R. oryzae CCS01 make new type cereal makgeolli.

Isolation and Characterization of White Spot Syndrome Baculovirus in Cultured Penaeid Shrimp (Penaeus chinensis) (양식새우(Penaeus chinensis)에서의 White Spot Baculovirus의 분리 및 특성)

  • Heo, M.S.;Sohn, S.G.;Sim, D.S.;Kim, J.W.;Park, M.A.;Lee, J.S.;Choi, D.L.;Jung, S.H.;Kim, Y.J.;Oh, M.J.
    • Journal of fish pathology
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    • v.13 no.1
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    • pp.7-13
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    • 2000
  • Beginning in the summer of 1993, a serious mortality among cultured penaeid shrimp occurred in the western sea of Korea. The typical sign of this disease was white spots inside the surface of the carapace. Cytopathic effect (CPE) were not observed by virus in CHSE-214, RTG-2, but not by pH 11. A nonoccluded rod-shaped form virus was observed by electron microscopy in the lymphoid organ. The virion was bacilliform virus and sourrounded by a virion envelope. Its virion protein was found to be similar to hypodermal and hematopoietic necrosis virus (HHNBV) by analysis of virion proteins in SDS-PAGE. The genome of virus is double stranded DNA molecule whose full length was about 114kb. It was similar to penaeus acute viremia (PAV) of Japan.

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Genomic DNA probe and purification of Theileria sergenti merozoites in Korean cattle (한우에 감염된 Theileria sergenti merozoite의 순수분리와 genomic DNA probe에 관한 연구)

  • Chae, Joon-seok;Lee, Joo-mook;Kwon, Oh-deog;Chae, Keon-sang
    • Korean Journal of Veterinary Research
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    • v.34 no.2
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    • pp.387-394
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    • 1994
  • To make the genomic DNA probe of Theileria sergenti, the merozoites were purified from bovine erythrocytes. The infected erythrocytes were lysed by Aeromonas hydrophila(Ah-1) hemolysin, and the parasites were isolated by ultracentrifugation on a Percoll discontinuous density gradient. For construction of a T sergenti genomic DNA library, T sergenti DNA was digested with Pstl and the fragments were ligated into the PstI site of pUC19 before transformation of Escherichia coli JM83. Out of thousands of transformants obtained by transformation of E coli JM83 with the genomic library, three plasmids were chosen. The sizes of the inserted DNAs were 2.9kb(2.4kb and 0.5kb) in pKTS1, 4.3kb in pKTS2 and 1.5kb in pKTS3, respectively. The DNA fragments used as probe KTS1(2.4kb), KTS2(4.3kb) and KTS3(1.5kb) were labeled digoxigenin-11-dUTP for the Southern hybridization. In Southern hybridization, all of the probes(KTS1, KTS2 and KTS3) reacted specifically to T sergenti DNA, but not to bovine leucocyte DNA. In order to find out the sensitivities of the digoxigenin-11-dUTP-labeled KTS1 and KTS3 as the probes, purified merozoite DNA and bovine DNA (control) were checked by dot blot hybridization with the probes. Both of the probes, KTS1 and KTS3, detected as minimum amount of 975pg of the T sergenti DNA, but not bovine DNA even to 500ng.

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Perspectives for the Industrial Use of Bacteriocin in Dairy and Meat Industry (축산업 분야에서의 박테리오신의 산업적 이용 및 향후 전망)

  • Lee, Na-Kyoung;Lee, Joo-Yeon;Kwak, Hyung-Geun;Paik, Hyun-Dong
    • Food Science of Animal Resources
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    • v.28 no.1
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    • pp.1-8
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    • 2008
  • More safe and natural food was recently needed by consumers. Antimicrobials including sodium azide, penicillin, and vancomycin were used for therapeutic agents against pathogens such as Listeria monocytogenes, Staphylococcus aureus, Escherichia coli O157:H7 in dairy and meat industry. These antimicrobials and preservatives were prohibited in stock farm and food because they were caused resistant strain and side effects. Bacteriocins are proteinaceous compounds that may present antimicrobial activity towards important food-borne pathogens and spoilage-related microflora. Therefore, bacteriocins were reported as an alternative of antimicrobials. Due to these properties, bacteriocin-producing strains or purified bacteriocins have a great potential of use in biologically based food preservation systems. Despite the growing number of articles regarding on the isolation of bacteriocinogenic strains, genetic determinants for production, purification and biochemical characterization of these inhibitory substances, there are only limited reports of successful application of bacteriocins to dairy and meats. This review describes bacteriocins related to dairy and meat products for the further use.

Intralobar Pulmonary Sequestration Receiving Its Blood Supply from the Celiac Artery (복강 동맥에서 혈액 공급을 받는 엽내 폐 분리증 1예)

  • Jung, Ki-Hwan;Lee, Seung-Hwa;Lee, Ju-Han;Jo, Won-Min;Shin, Chol;Kim, Je-Hyeong
    • Tuberculosis and Respiratory Diseases
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    • v.68 no.6
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    • pp.358-362
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    • 2010
  • Intralobar pulmonary sequestration is a rare congenital lung anomaly. It is defined as a portion of nonfunctioning lung parenchyma that receives its blood supply from an anomalous systemic artery. Patients often present with chronic or recurrent pneumonia. A chest radiograph may show a cystic lesion with air-fluid levels in the lung base. A high index of suspicion is needed for a diagnosis. Surgical removal of a symptomatic intralobar pulmonary sequestration is generally the treatment of choice. Identifying the aberrant artery is a difficult problem when resecting a pulmonary sequestration. The thoracic and abdominal aortas are the most common origins for the abnormal blood supply. However, arterial supply from the celiac artery is quite rare. We present a case of intralobar pulmonary sequestration with the blood supply originating from the celiac artery.

A Study on Input Multiplexer for Ku-Band Satellite Transponder (Ku 대역 위성 중계기용 입력 멀티플렉서에 관한 연구)

  • 이주섭;엄만석;염인복;이성팔
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.14 no.4
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    • pp.393-400
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    • 2003
  • This paper deals with the design and manufacturing technique of EQM(Engineering Qualification Model) of input multiplexer(IMUX) for the Ku-band satellite transponder. Channel dropping method by circulator chain structure is adopted for demultiplexing each channel. External equalizers are attached behind channel filters fur reduction of group delay variation and amplitude variation simultaneously. Both channel filters and equalizers adopted dual-mode technique in design f3r mass and volume reduction. Channel filters are designed to have 8-pole elliptic response and equalizers to be of 2-pole reflection type. For good temperature stability characteristics, INVAR36 material is used for channel filters and external equalizers. Vibration test, Thermal Vacuum Test, and EMC test have been performed on input multiplexer and it is shown to be suitable for Ku-band satellite transponder.

Quality Properties of Cakes Containing Gamma-Irradiated Egg White (감마선 조사된 난백 함유 Cake의 품질 특성 - 연구노트 -)

  • 이주운;서지현;김영호;최정미;육홍선;안현주;변명우
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.2
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    • pp.311-314
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    • 2003
  • As a research on the practical approaches of gamma irradiation for the reduction of egg allergy, cakes including gamma-irradiated egg white were manufactured, and rheological characteristics and sensory qualities of the cakes were evaluated. Egg white was separated from whole egg and then gamma-irradiated with the absorbed dose of 10 or 20 kGy White layer cake, pound cake and sponge cake were made with irradiated egg white and used to the subsequent experiments. Firmness of all samples containing irradiated egg white was higher than that of control. Retrogradation of pound and sponge cakes containing irradiated egg white was delayed, and the result showed that the usage of irradiated egg white was better at the point of rheological storage ability. Effects of irradiated egg white on the sensory quality were different depending onto the properties of each product. Radiation off-odor was observed in the a11 samples containing irradiated egg white. To maintain the sensory Qualify, adequate methods such as masking effects should be developed during manufacture.