• Title/Summary/Keyword: 분리.이주

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Isolation of Listeria monocytogenes from animal carcasses and environmental specimens in slaughter house level (도축처리 단계별 도체 및 환경재료에서 Listeria monocytogenes의 분리)

  • 허정호;손성기;이주홍;임삼규;구정헌;박영호;조명희;손원근;강호조
    • Korean Journal of Veterinary Service
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    • v.20 no.1
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    • pp.69-77
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    • 1997
  • To invastigate the epidemiological trait of listeriosis, Listeria monocytogenes were isolated from the carcasses of pigs and cattle, and environmental specimens in slaughter house. Also serotype of isolates were classified by rapid slide agglutination test. In the carcasses of pigs, Listeria sp were isolated from the carcasses after bleeding(62%), after dismemberment(60.0%) and before shipping(76.0%), and L monocytogenes were present in 8% of the carcasses after dismemberment and in 14% of the carcasses before shipping. However, few Listeria sp were isolated from the living body skin and the carcasses after scalding. In the carcasses of cattle, Listeria sp were isolated from the carcasses after bleeding(10%), after dismemberment(36.7%) and before shipping(63.3%), L monocytogenes were present in 3.3% of the carcasses after dismemberment and in 10% of the carcasses before shipping. Overall, L monocytogenes, L innocua, L welshimen, L grayi, and L murrayi were present In 4.8, 40, 2.3, 2.6 and 0.3% of all the carcasses, respectively. Prevalence of Listeria sp in environmental specimens were found to be 80% in slaughter house floors and 100% in sewage, and L monocytogenes were present in 15% of sewage. However, few Listeria sp were isolated from chilled water and from scalding water. Overall, L monocytogenes, L innocua, and L welshimeri were present in 3.8, 45 and 6.3% of all the environmental specimens, respectively. A total 27 strains of L monocytogenes were isolated from samples tested and all of the strains were classified into serotype 1.

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Antibiotic Resistance of Bacterial Isolates from Nasal Discharges of Dogs with Respiratory Diseases (개의 비루에서 분리한 원인균의 항생제 내성)

  • 김문선;정종태;강태영;윤영민;이주명;이두식;손원근
    • Journal of Veterinary Clinics
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    • v.21 no.2
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    • pp.133-139
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    • 2004
  • Bacterial pathogens were isolated from 36 dogs with respiratory signs, that were submitted to Veterinary Clinics in Jeju, including Veterinary Medical Teaching Hospital in Cheju National University. Of 36 isolates, 16 (44.4%) bacterial pathogens were Gram-positive and 20 (55.6%) were Gram-negative. Gram-positive bacteria identified with API Staph were 12 S. intermedius (33.3%), 2 S. aureus (5.6%), 1 S. haemolyticum (2.8%), and 1 S. xylosus (2.8%). Gram-negative organisms identified with API 20E or API NE included 8 Bordetella bronchiseptica (22.2%), 6 Escherichia coli (16.7%), 4 Pasteurella spp. (11.1%), 1 Enterobacter intermedius (2.8%), and 1 Oligella ureolytica (2.8%). Both Staphylococcus spp. isolates and Gram-negative pathogens were resistant to one or more antibiotics, including ampicillin (AM), amoxicillin/clavulanic acid (AMC), chloramphenicol (C), cefazolin (CZ), erythromycin (E), gentamicin (GM), kanamycin (K), lincomycin (L), oxacillin (OX), trimethoprim/sulfamethoxazole (SXT), and tetracycline (TE). All Staphylococcus spp. were susceptible to AMC, OX and VA, while many isolates were highly resistant to L (87.5%), E (68.8%), P (62.5%), and AM (56.3%). Antibiotic-resistant patterns of staphylococcal isolates were shown ranges from single to 9-resistant patterns. Resistant rates to antibiotics of Gram-negative bacteria were usually higher than those of Staphylococcus spp. in this study. Most Gram-negative bacteria were highly resistant to L (90.0%), AM (85.0%), E (85.0%), P (85.0%), OX (80.0%), and CZ (75.0%). B. bronchiseptica isolates showed 5 to 8 antibiotics-resistant patterns and Pasteurella spp., 2 to 8-resistant patterns. In particular, all 6 E. coli isolates were resistant to more than 9 different kinds of antibiotics, including one strain resistant to all antibiotics tested.

Robot vision system for face tracking using color information from video images (로봇의 시각시스템을 위한 동영상에서 칼라정보를 이용한 얼굴 추적)

  • Jung, Haing-Sup;Lee, Joo-Shin
    • Journal of Advanced Navigation Technology
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    • v.14 no.4
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    • pp.553-561
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    • 2010
  • This paper proposed the face tracking method which can be effectively applied to the robot's vision system. The proposed algorithm tracks the facial areas after detecting the area of video motion. Movement detection of video images is done by using median filter and erosion and dilation operation as a method for removing noise, after getting the different images using two continual frames. To extract the skin color from the moving area, the color information of sample images is used. The skin color region and the background area are separated by evaluating the similarity by generating membership functions by using MIN-MAX values as fuzzy data. For the face candidate region, the eyes are detected from C channel of color space CMY, and the mouth from Q channel of color space YIQ. The face region is tracked seeking the features of the eyes and the mouth detected from knowledge-base. Experiment includes 1,500 frames of the video images from 10 subjects, 150 frames per subject. The result shows 95.7% of detection rate (the motion areas of 1,435 frames are detected) and 97.6% of good face tracking result (1,401 faces are tracked).

Assessment of Economic Value of Youngsan River Estuary (영산강 하구역의 경제적 가치 평가)

  • Yoo, Seung-Hoon;Lee, Joo-Suk
    • Journal of Korea Water Resources Association
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    • v.44 no.8
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    • pp.629-637
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    • 2011
  • This study tried to estimate the environmental value of Youngsan river estuary as there are conflicts on the management policy about Youngsan river estuary. For estimating the detailed functional values of Youngsan river estuary, this study used the contingent valuation method with multi attribute utility theory for. In addition, the survey was separately practiced to on-site and off-site. According to estimating results, the annual value of Youngsan river estuary is about 27.4 billion Korean won. The results of this study are expected to contribute to determine the appropriate level of investment and management policies with regard to the estuary and to provide methodological guidelines for follow-up study.

ROI Extraction and Enhancement for Finger Vein Recognition (지정맥 인식을 위한 ROI 검출과 정맥 증강처리)

  • Lee, Ju-Won;Lee, Byeong-Ro
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.19 no.4
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    • pp.948-953
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    • 2015
  • Recently, the finger vein recognition based on NIR and CCD sensor camera is investigating the technology to identify a personal using by biometrics. The performance difference of finger vein recognition is generated according to methods that are to separate the vein and background from noises such as finger thickness, ambient light, skin temperature, etc. To improve these problems, in this study, we are proposing the methods for rotation, ROI extraction, and enhancement of vein image captured by NIR LED and CCD camera, and were evaluated performances of these methods. In results of the experiment, the accuracy of the proposed method for image rotation and ROI extraction was 99.8%. And the proposed filter bank method in vein enhancement has shown better performance than retinex algorithm. The proposed method for results of these experimentations will provide better recognition rate when applied to the preprocessing of finger vein recognition.

A Study of $SF_6$ Treatment using Principles of Gas Hydrate Formation (가스 하이드레이트 형성 원리를 이용한 $SF_6$ 처리 기술에 관한 연구)

  • Lee, Bo-Ram;Lee, Hyun-Ju;Kim, Yang-Do;Ryu, Young-Bok;Lee, Man-Sik;Kim, Young-Seok;Lee, Ju-Dong
    • 한국신재생에너지학회:학술대회논문집
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    • 2007.06a
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    • pp.485-488
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    • 2007
  • $SF_6$(sulfur hexafluoride)는 뛰어난 단열 및 아크방지능력(arc-extinguishingproperty)으로 인해, 전력용 변압기의 절연가스와 반도체${\cdot}$액정용 플라즈마 CVD로의 cleaning gas, 주물공장 covering gas 등으로 사용되고 있다. 하지만, $SF_6$의 지구온난화지수(global warming potential)는 $CO_2$대비 23,900배가 높아 기후변화에 미치는 영향이 $CO_2$보다 훨씬 크고, 대기 중 분해되지 않고 잔존하는 기간이 $CH_4$ 10년, $CO_2$ 및 CFCs는 100년으로 추정되는데 반해, $SF_6$는 3,200년으로 연간방출양이 작더라도 오랜 기간 누적되면 그 파장이 클 것으로 사료된다. 대부분의 가스 하이드레이트(고상결정상태)는 고압, 저온에서 형성가능 하지만, 불화가스에 대해서는 쉽게 결정화가 일어난다. $SF_6$는 3$^{\circ}C$, 2기압에서 고밀도 고상화가 되기 때문에 여러 기체와 흔합되어 있는 경우 $SF_6$만을 압축된 고상 결정상태를 형성, $SF_6$를 회수, 정제할 수 있으므로 불화가스 분리${\cdot}$회수에 기술적, 경제적 효과를 기대할 수 있다. 본 연구에서는 하이드레이트 촉진제로서 계면활성제(promoter) 첨가에 따른 $SF_6$ 하이드레이트 형성 및 해리과정 실험을 통해 효율적인 $SF_6$ 저감에 관한 적용기술을 연구해 보았다.

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A Critical Approach on Multiculturalism Shown in Romance Films (로맨스 영화에서 다루어지는 다문화주의에 대한 비판적 고찰)

  • Oh, Sang-Hee;Lee, Joo-Eun
    • The Journal of the Korea Contents Association
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    • v.15 no.8
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    • pp.156-169
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    • 2015
  • The negative awareness of a multiculturalism rises around the world because of various problems that happen with the multicultural phenomenon. Nevertheless, the reason we uphold multiculturalism is that multicultural phenomenon is an inevitable consequence of capitalism and the decline of multiculturalism might imply danger of bringing about radical nationalism. This study assumes that a distortion of multiculturalism is the result from a myth of romance film which affects the public perception as an integral component of a discourse. Grounded upon monomith, the narrative structure of romance film of which motive comes from courtly love makes people miscomprehend the value of multiculturalism because of the multicultural factor as substitute for romantic obstacle. As the character of romance movie is likely to be formed focusing on superficial images and denotations, this tendency causes the hierarchy and the representative minority drives out the rest of other minorities. The attempt to arouse people's tolerance and understanding of the other is frustrated both by the structure of binary oppositions and the clich$\acute{e}$.

Solar Array Deployment Analysis of a Satellite (인공위성 태양전지판 전개해석)

  • Kim, Kyung-Won;Kim, Sun-Won;Lim, Jae-Hyuk;Rhee, Ju-Hun;Hwang, Do-Soon;Jin, Ik-Min;Kim, Hak-Jung;Song, Woon-Hyung;Choi, Hang-Suk
    • Journal of Satellite, Information and Communications
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    • v.3 no.1
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    • pp.29-34
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    • 2008
  • After spacecraft is separated from the launch vehicle, first of all spacecraft deploy the solar array. Solar array deployment is one of the key factors deciding the success of the spacecraft mission. Therefore, It is necessary to predict the solar array deployment motion and check the safety through calculating the load on the tape hinges of solar array using the deployment analysis in the initial design phase. In this paper, solar array deployment analysis is performed by multi-body dynamics simulation program. From the analysis results, assessment on the safety also is carried out. In addition, hinge characteristic test is fulfilled to find out hinge characteristic, and is applied to the deployment analysis.

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Anticandidal Activity of the Protein Substance from Coptidis Rhizoma (황련에서 분리된 단백질성분의 항진균효과)

  • Kim Hyunkyung;Lee Jue-Hee;Shim Jin Kie;Han Yongmoon
    • YAKHAK HOEJI
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    • v.49 no.4
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    • pp.323-329
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    • 2005
  • Antimicrobial peptides are evolutionary ancient weapons for animal and plant species to depend themselves against infectious microbes. In the present study, we investigated if an antimicrobial peptide was produced from Coptidis Rhizoma. For the determination, protein substance from the medicinal plant was isolated by various preparations. Among the preparations, the protein portion dissolved in phosphate-buffered saline solution (CRP-DS) that contained the most amount of protein $(90\%)$ resulted in maximal inhibition of Candida albicans which causes local and systemic infections. Analyses by gel-electrophoresis and gel-permeation chromatography showed the CRP-DS formed a single band of approximately 11.8 KDa as molecular size. Antifungal activity of the CRP-DS was almost equivalent to antifungal activity by fluconazole, resulting in MIC (minimal inhibitory concentration) of approximately $50{\mu}g/ml$. The antifungal activity was a dose-dependent. The antifungal activity appeared to be inactivated by heat-treatment and ionic strength, respectively. In a murine model, the CRP-DS enhanced resistance of mice against disseminated candidiasis. The HPLC analysis demonstrated maximum $4\%$ of berberine as residual content in the CRP-DS preparation resulted in no influence on the antifungal activity. In addition, protein portion isolated from Phellodendri Cortex producing the alkaloid component like Coptidis Rhizoma had no such anticandidal effect. These results indicate that the protein substance from Coptidis Rhizoma was responsible for the antifungal activity.

Isolation and Antimicrobial Susceptibility of Microorganisms from Milk Samples of Jindo Dogs (Canis familiaris var. jindo) (진도견(Canis familiaris var. jindo) 유즙으로부터 분리한 세균의 분포 및 항균제 감수성)

  • Lee, Ju-Dan;Lee, Youn-Kyong;O, Suck-Il;Jung, Ji-Young;Son, Chang-Ho;Shin, Sung-Shik;Oh, Ki-Seok;Hur, Tai-Young;Suh, Guk-Hyun
    • Korean Journal of Veterinary Research
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    • v.51 no.1
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    • pp.29-35
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    • 2011
  • Lack of hygiene and puerperal mastitis are common causes of bacterial diseases in nursing neonates. The aim of this study was to isolate microorganisms from milk samples of healthy female Jindo dogs with suckling puppies and to investigate antimicrobial susceptibility against the isolated bacteria. Milk samples were collected from 120 udders of 12 lactating Jindo dogs that were 2~4 years old without any clinical diseases including mastitis. Bacteria were isolated from 64 milk samples (53.3%), either singly (76.6%) or in combination (23.4%). Staphylococcus (S.) spp. was the most common microorganisms (74.7%) isolated from canine milk, followed by Haemophillus spp. (10.9%), Streptococcus spp. (9.6%), Gardnerella spp. (2.4%) and Moraxella spp. (2.4%). The most frequently isolated organism was S. warneri (31.3%). Antimicrobial susceptibility of these bacteria was tested with 17 antimicrobial agents by Kirbyand Bauer standardized disc diffusion method. Results indicated that bacteria isolated from healthy canine milk were mostly susceptible to amoxicillin-clavulanic acid, cephalothin and ceftiofur, but were resistant to erythromycin, neomycin and tetracycline.