• Journal of Korean Society of Forest Science
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• v.102 no.1
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• pp.59-65
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• 2013

The jujube is an important fruit tree species in Korea. Traditionally, classifications of jujube cultivars have been based on morphological characters; however, morphological identification can be problematic because morphological traits are affected by environmental conditions. Therefore, DNA markers are now being used for the rapid and accurate identification of plant species. Inter-simple sequence repeat (I-SSR) is one of the best DNA-based molecular marker techniques, which is useful for studying genetic relations and for the identification of closely related cultivars. In this study, 5 Korean jujube trees and 1 jujube tree imported from China were analyzed for 16 I-SSR primers. Amplification of the genomic DNA of jujube cultivars by using I-SSR analysis generated 100 bands, with an average of 6.25 bands per primer, of which 45 bands (45%) were polymorphic. The number of amplified fragments with I-SSR primers ranged from 2 to 13. The percentage of polymorphism ranged from 10% to 100%. I-SSR finger printing profiles showed that 'Boeun jujube' and 'Daeri jujube' had characteristic DNA patterns, indicating unequivocal cultivar identification at molecular level. According to the results of clustering analysis, the genetic similarity coefficient ranged from 0.68 to 0.92. 'Boeun jujube' and 'Daeri jujube' were divided into independent groups, and 'Bokjo jujube', 'Geumseong jujube', 'Wolchul jujube', and 'Mudeung jujube' were placed in the same group. Therefore, I-SSR markers are suitable for the discrimination of 'Boeun jujube' and 'Daeri jujube' cultivars.

• Korean Journal of Plant Resources
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• v.19 no.1
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• pp.105-111
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• 2006

The objectives of this study, an analysis of fruit and leaf morphological characteristics among the five Zizyphus cultivars could be used for the investigation of cultivars classification and could provide information to make out the UPOV TG(Test Guidelines). ANOVA tests showed that there were statistically significant differences in all fruit and leaf morphological characteristics among the five Zizyphus cultivars at 1% level. But, for kernel characteristics, differences were statistically non-significant among the cultivars. Approximately, the Wolchul and Boeun cultivars showed larger and smaller values in overall characteristics and cultivars, respectively. The results of principal component analysis(PCA) for the fruit and leaf morphological characteristics showed that the first for principal components(PC's) explained about 65.3% of the total variation. The first PC was correlated with those characteristics that were mainly related to the terminal leaf length(TLL), leaf length(LL), fruit length(FL), terminal leaf width(TLW), and leaf petiole length(LPL). The second and third PC was mainly correlated with the terminal leaf morphological index(TLMI). Therefore, these characteristics were important to analysis of the fruit and leaf morphological characteristics and classification among the five Zizyphus cultivars. Cluster analysis using UPGMA method based on principal components showed that five Zizyphus cultivars could be clustered into two groups. Group I comprises Mudung, Wolchul, and Bokjo and Geumsung cultivars, Group II is Boeun cultivar. These results well similar to that of principal component analysis.

• Journal of Life Science
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• v.27 no.12
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• pp.1470-1478
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• 2017

The aim of this study was to investigate biological activity and biochemical properties of extracts from Bacillus subtilis-fermented silkworm (Bombyx mori L., SP) powder of different origin (Buan, Namwon, and Boeun). An additional aim was to determine the inhibition of cancer cell (B16-F10, HT-29, LNcaP, and MCF-7) proliferation and nitric oxide (NO) production from lipopolysaccharide (LPS)-induced RAW264.7 cells. Biological activities (${\alpha},{\alpha}^{\prime}$-diphenyl-${\beta}$-picrylhydrazyl [DPPH], free radical scavenging activity, fibrinolytic activity, antiproliferation activity, and anti-inflammatory activity) and biochemical properties (compositional amino acid contents, and mineral contents) were examined in water extracts from silkworm powder and B. subtilis-fermented silkworm powder. The highest amino acid contents were detected in Buan silkworm powder (BU). After fermented, the highest contents were found in B. subtilis-fermented Buan silkworm powder (BBO). The major minerals detected were K, Ca, and Mg. Rates of these minerals, especially those of Na increased after fermented. DPPH radical scavenging activity and fibrinolytic activity were stronger in the fermented group than non-fermented group. DPPH radical scavenging activity and fibrinolytic activity were highest in the extract from BBO. The inhibition activities of LNcaP and MCF-7 cells viability were significantly decreased in the BBO, and there was no inhibition activity in other cancer cells (B16-F10 and HT-29). An SRB assay of the cell viability of RAW 264.7 cells exposed to extracts of silkworm powder and B. subtilis-fermented silkworm powder revealed no toxicity in any of the groups. Compared with the LPS-treated group, the biggest reduction in NO production was detected in the BBO group. Based on these results, extracts from Boeun silkworm powder fermented with B. subtilis could be a candidate material as a dietary supplement for use in healthy functional foods.

• 대한예방의학회:학술대회논문집
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• 2001.10a
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• pp.462-463
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• 2001

• 대한예방의학회:학술대회논문집
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• 2001.10a
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• pp.409-410
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• 2001

• 대한예방의학회:학술대회논문집
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• 2001.10a
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• pp.267-269
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• 2001

• 건강소식
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• v.34 no.4
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• pp.28-31
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• 2010

오리숲길 소나무가 하늘을 가렸다. 상쾌한 솔향기에 온몸이 싱그러워진다. 걷다가 만난 황톳길을 맨발로 걷는다. 오리숲길 중간에 있는 일주문을 지나 자연관찰로로 접어든다. 그 길에 조릿대와 생강나무, 전나무, 신갈나무, 소나무 등이 빽빽하게 들어섰다. 흙길이 부드럽다. 법주사에서 마음을 씻어내고 되돌아 나오는 길, 속리산 자락에서 나는 산나물 산약초로 만든 비빔밥으로 풋풋한 봄 맛을 느껴볼 일이다.

• 정보화사회
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• s.151
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• pp.58-61
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• 2001

한국정보통신산업협회에서는 지난 1995년부터 정부 정보통신부문 공식 통계 승인기관으로 정보통신부문 산업통계조사를 지속해 왔다. 본 실태조사는 2001년 3월 1일까지 교육인적자원부에 등록된 4년제 대학 186개교 중 2001년 4월 6일부터 5월 16일까지 정보통신기술인력의 기초 현황조사에 응한 정보통신부문 대학을 포함하는 143개 대학교를 대상으로 한 ‘정보통신기술인력 실태 조사’ 결과이다. 단, 무응답(non response)대학은 Sequetial Hot-Deck Imputation방법을 통해서 보정하였다.

• 대한예방의학회:학술대회논문집
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• 1999.10a
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• pp.183-184
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• 1999

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 1995.04a
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• pp.43-46
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• 1995

비가림 시설은 단순한 재배시설로 시공이 간편하며 경제적이며 보은-난방이 불필요한 하절기용으로 많이 사용된다. 간이시설은 단순한 형태이면서도 기본적으로 실내 온도가 재배작물의 생육한계 온도 이하가 되도록 하여야 하고, 강우 차단을 위하여 소요되는 인력을 최소한으로 할 수 있어야 하며, 또한 하절기 강풍에 대하여 구조적으로 안전하여야 한다. 따라서 우수한 환기특성과 적절한 작업성을 유지하면서 강우차단효과를 가질 수 있는 효율적인 간이시설의 형태 개발이 필요하다. (중략)