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http://dx.doi.org/10.14578/jkfs.2013.102.1.059

Assessment of Genetic Relationship among Date (Zizyphus jujuba) Cultivars Revealed by I-SSR Marker  

Nam, Jae-Ik (Department of Forest Science, Chungbuk National University)
Kim, Young-Mi (Department of Forest Science, Chungbuk National University)
Choi, Go-Eun (Department of Forest Science, Chungbuk National University)
Lee, Gwi-Young (Forest Environment Research Institute)
Park, Jae-In (Department of Forest Science, Chungbuk National University)
Publication Information
Journal of Korean Society of Forest Science / v.102, no.1, 2013 , pp. 59-65 More about this Journal
Abstract
The jujube is an important fruit tree species in Korea. Traditionally, classifications of jujube cultivars have been based on morphological characters; however, morphological identification can be problematic because morphological traits are affected by environmental conditions. Therefore, DNA markers are now being used for the rapid and accurate identification of plant species. Inter-simple sequence repeat (I-SSR) is one of the best DNA-based molecular marker techniques, which is useful for studying genetic relations and for the identification of closely related cultivars. In this study, 5 Korean jujube trees and 1 jujube tree imported from China were analyzed for 16 I-SSR primers. Amplification of the genomic DNA of jujube cultivars by using I-SSR analysis generated 100 bands, with an average of 6.25 bands per primer, of which 45 bands (45%) were polymorphic. The number of amplified fragments with I-SSR primers ranged from 2 to 13. The percentage of polymorphism ranged from 10% to 100%. I-SSR finger printing profiles showed that 'Boeun jujube' and 'Daeri jujube' had characteristic DNA patterns, indicating unequivocal cultivar identification at molecular level. According to the results of clustering analysis, the genetic similarity coefficient ranged from 0.68 to 0.92. 'Boeun jujube' and 'Daeri jujube' were divided into independent groups, and 'Bokjo jujube', 'Geumseong jujube', 'Wolchul jujube', and 'Mudeung jujube' were placed in the same group. Therefore, I-SSR markers are suitable for the discrimination of 'Boeun jujube' and 'Daeri jujube' cultivars.
Keywords
Zizyphus jujuba; jujube tree; I-SSR; cultivar identification; molecular marker; genetic relationship;
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