• Journal of the Korean Society of Food Science and Nutrition
• /
• v.37 no.10
• /
• pp.1337-1342
• /
• 2008

This study was conducted to investigate effects of chemical sanitizers on inhibiting foodborne pathogens, such as Listeria monocytogenes (L. monocytogenes), Salmonella Typhimurium (S. Typhimurium), and E scherichia coli O157:H7 (E. coli O157:H7), on cabbages. Cabbages were inoculated with the culture cocktail of pathogens and treated with water, 100 ppm commercial chlorine, and 50, 100, and 200 ppm chlorine dioxide ($ClO_2$) for 1, 5, and 10 min at room temperature ($22{\pm}2^{\circ}C$). Treatments with water did not significantly reduce levels of three pathogens whereas other treatments with chemical sanitizers significantly reduced levels of three pathogens. Treatment with 200 ppm $ClO_2$ for 10 min was the most effective at inhibiting pathogens and reduction levels were 1.90, 1.92, and 1.98 log CFU/g for L. monocytogens, S. Typhimurium, and E. coli O157:H7, respectively. Levels of reduction were increased with the increase of $ClO_2$ concentrations. When chemically injured cells were investigated, there were no significant differences on the levels of injured cells between before and after treatment with commercial chlorine and $ClO_2$. These results suggest that $ClO_2$ can be used as an alternative sanitizer for reducing pathogens on fresh produces.

• Journal of Food Hygiene and Safety
• /
• v.22 no.4
• /
• pp.235-242
• /
• 2007

This study was conducted to investigate the effect of aerosolized chemical sanitizers on inhibiting foodborne pathogens such as Escherichia coli O157:H7, Salmonella typhimurium, and Listeria monocytogenes. Five domestic commercial sanitizers subjected to five groups of sanitizer (chlorine-based, hydrogen peroxide-based, Iodophor-based, quaternary ammonium-based, and alcohol-based sanitizers) were aerosolized by an aerosol generator into a model cabinet and treated in laboratory media containing three pathogens for 1 h at room temperature. Aerosolized hydrogen peroxide-based and quaternary ammonium-based sanitizers were effective at inhibiting levels of E. coli O157:H7 (ca. 4-9 log reductions) whereas other aerosolized sanitizers such as chlorine-based, Iodophor-based, and alcohol-based sanitizers did not significantly reduced the levels of E. coli O157:H7. For S. typhimurium, the only aerosolized hydrogen peroxide-based sanitizer was effective and resulted in ca. 5-9 log reduction. Aerosolized hydrogen peroxide-based, Iodophor-based, and quaternary ammonium-based sanitizers significantly reduced levels of L. monocytogenes and especially, aerosolized quaternary ammonium-based sanitizer was strongly effective to kill L. monocytogenes, resulted in higher than 8.8 log reduction. And there was no special trend in inhibitory efficacy of sanitizers aerosolized by 1.6 or 2.4 MHz aerosol generators. From these results, aerosolization has great potential for use in commercial applications however it's efficacy could be very different depending on type of sanitizers.

• Proceedings of the KSAR Conference
• /
• 2002.06a
• /
• pp.195.1-195
• /
• 2002

• The Microorganisms and Industry
• /
• v.32 no.2
• /
• pp.13-15
• /
• 2006

• The Microorganisms and Industry
• /
• v.35 no.1
• /
• pp.10-13
• /
• 2009

• The Microorganisms and Industry
• /
• v.30 no.2
• /
• pp.21-30
• /
• 2004

• Journal of Korean Society of Environmental Engineers
• /
• v.32 no.11
• /
• pp.1063-1068
• /
• 2010

This study compared UV and UV/$H_2O_2$ inactivation of E.coli, a possible indicator microorganism for fecal contamination of water, and $Q{\ss}$ phage, an indicator for pathogenic viruses. UV inactivation of $Q{\ss}$, T4 and lambda phages in actual secondary effluent was investigated, too. As a result, similar inactivation efficiency between $Q{\ss}$ phage and E.coli was observed during UV treatment, while $Q{\ss}$ phage showed higher resistance to UV/$H_2O_2$ than E.coli. $Q{\ss}$ phage resistance to UV or UV/$H_2O_2$ does not reflect those of all pathogenic viruses. However, the result tells that the use of E.coli inactivation efficiency in evaluating microbiological safety of water could not always ensure the sufficient safety from pathogenic viruses. Meanwhile, $Q{\ss}$ phage showed less resistance to UV than T4 and lambda phages, indicating that the use of $Q{\ss}$ phage as an indicator virus may bring insufficient disinfection effectiveness by causing the introduction of lower UV dose than required. Consequently, it can be thought that T4 or lambda phages would be more desirable indicators in ensuring the sufficient disinfection effectiveness for various pathogenic viruses.

• Proceedings of the Korean Society of Fisheries Technology Conference
• /
• 2000.05a
• /
• pp.444-445
• /
• 2000

계절, 서식 환경의 구분없이 발병하는 병원성 미생물로 인하여 어류의 감염피해 및 경제적 손실이 막대하며 병원성 미생물을 저해하기 위해 투여한 항생제의 오남용으로 인하여 내성을 갖는 세균들의 출현 위험성이 상재하고 있다. 따라서 항생제 사용에 대한 효과 감소는 물론 과량 투여에 따른 양식업의 경쟁력 약화로 이루어질 가능성이 높기 때문에 보다 환경 친화적이며 어병의 효율적인 생물학적 제어를 시도하기 위해 어류의 gastrointestinal(GI) track으로부터 수산용 probiotic 유산균을 분리 동정하고 그 특성을 조사하여 앞으로 수산용 probiotic으로서 이용가능성을 알아보고자 하였다. (중략)

• Journal of Animal Science and Technology
• /
• v.48 no.5
• /
• pp.709-718
• /
• 2006

In order to know the effects of Garlic, Scallion, Flavonoid, Urushiol, Anthocyanidin and Bio-MOS?? on pathogenic microbes and rumen anaerobic microbes, the growth rate of pathogens (including Escherichia coli O157, Salmonella paratyphi, Listeria monocytogenes and Staphylococcus aureus) and in vitro rumen microbial growth, gas production, ammonia concentration, carboxymethylcellulase(CMCase) activity, and microbial populations were investigated.The growth of pathogens was inhibited by supplementation of 0.1% Flavonoid, Scallion or Bio-MOS?? as biological active materials. And Scallion and Flavonoid had powerful antimicrobial properties on the pathogens applied in paper disc method.Although few effects by biological active materials disappeared in rumen fermentation in vitro, CMCase activity removed with supplementation of 1% of Flavonoid which had antimicrobial property in paper disc method. Scallion, having powerful antimicrobial property on pathogens and no inhibiting on rumen fermentation, might be a source in development of natural antimicrobial agent for ruminants.

• Journal of Korean Society of Environmental Engineers
• /
• v.29 no.9
• /
• pp.1020-1026
• /
• 2007

The control of pathogenic microorganisms is important issue in human environments, especially in surface area. However, surface disinfection has not been fully researched. In this study, the surface disinfection under UV irradiation was performed to investigate the quantitative kinetics for Bacillus subtilis spore inactivation in several experimental conditions, such as light intensity, temperature and surface roughness. This study reports that UV light would apparently inactivates the microorganisms and the required IT value for 2 log (99%) Bacillus subtilis spore inactivation was found to be 14.5 $mJ/cm^2$ in plain surface, as predicted by the Delayed Chick-Watson model. When UV was irradiated, there were no significant temperature effects. However, the experimental result shows that the more increased IT values are required at larger surface roughness.