UV 불활성화 효과에 의거한 E.coli, RNA 및 DNA 박테리오파지의 대체 지표 미생물로서의 적용성 검토

Applicability Investigation of E.coli, RNA and DNA Bacteriophages for Possible Indicator Microorganisms Based on the Inactivation Effectiveness by UV

  • 김일호 (교토대학 대학원 공학연구과 부속 유역권 종합환경질 연구센터) ;
  • 와히드 마피아 (교토대학 대학원 공학연구과 부속 유역권 종합환경질 연구센터) ;
  • 타나카 히로아키 (교토대학 대학원 공학연구과 부속 유역권 종합환경질 연구센터)
  • Kim, Il-Ho (Research Center for Environmental Quality Management, Graduate School of Engineering, Kyoto University) ;
  • Wahid, Marfiah AB (Research Center for Environmental Quality Management, Graduate School of Engineering, Kyoto University) ;
  • Tanaka, Hiroaki (Research Center for Environmental Quality Management, Graduate School of Engineering, Kyoto University)
  • 투고 : 2010.08.24
  • 심사 : 2010.11.26
  • 발행 : 2010.11.30

초록

본 연구는 유효한 분변오염의 지표 미생물로서 제안되고 있는 E.coli와, 병원성 바이러스에 대한 지표 미생물로 추천되는 $Q{\ss}$ 파지의 UV 및 UV/$H_2O_2$에 대한 내성을 비교하였다. 이 결과에 의거, 병원성 바이러스 관리를 위한 E.coli 규제의 유효성을 고찰하였다. 또한, 병원성 바이러스에 대한 대체 지표 미생물로써, $Q{\ss}$ 파지, T4 및 lambda 파지의 실제 하수 2차 처리 수중에서의 UV 불활성화를 비교하였다. 실험결과, $Q{\ss}$ 파지와 E.coli는 UV에 대해 거의 동등한 내성을 보였으나, UV/$H_2O_2$ 공정에 대해서는 E.coli보다 $Q{\ss}$ 파지가 더 높은 내성을 보였다. 이로부터, $Q{\ss}$ 파지가 모든 병원성 바이러스의 UV 또는 UV/$H_2O_2$에 대한 내성을 대표한다고 할 수는 없을지라도, 지표 미생물로써 E.coli의 불활성화 효과에만 의존하는 것은, 소독공정에 따라서는 바이러스류에 대해 충분한 소독효과를 얻지 못할 수도 있음을 알 수 있었다. 한편, T4 및 lambda 등 DNA 파지와 불활성화를 비교하였을 경우, $Q{\ss}$ 파지가 UV에 대해 더 낮은 내성을 보였다. 따라서, 불활성화에 대한 지표 바이러스로써 $Q{\ss}$ 파지의 이용은 낮은 UV 조사량의 도입에 의해 결과적으로 불충분한 소독효과를 초래할 수도 있을 것 같다. 이 결과는, 병원성 바이러스류에 대해 보다 충분한 불활성화를 달성하기 위해서는 RNA 파지인 $Q{\ss}$보다 T4 및 lambda 등 DNA 파지가 지표 바이러스로써 보다 유효할 수 있음을 보여준다.

This study compared UV and UV/$H_2O_2$ inactivation of E.coli, a possible indicator microorganism for fecal contamination of water, and $Q{\ss}$ phage, an indicator for pathogenic viruses. UV inactivation of $Q{\ss}$, T4 and lambda phages in actual secondary effluent was investigated, too. As a result, similar inactivation efficiency between $Q{\ss}$ phage and E.coli was observed during UV treatment, while $Q{\ss}$ phage showed higher resistance to UV/$H_2O_2$ than E.coli. $Q{\ss}$ phage resistance to UV or UV/$H_2O_2$ does not reflect those of all pathogenic viruses. However, the result tells that the use of E.coli inactivation efficiency in evaluating microbiological safety of water could not always ensure the sufficient safety from pathogenic viruses. Meanwhile, $Q{\ss}$ phage showed less resistance to UV than T4 and lambda phages, indicating that the use of $Q{\ss}$ phage as an indicator virus may bring insufficient disinfection effectiveness by causing the introduction of lower UV dose than required. Consequently, it can be thought that T4 or lambda phages would be more desirable indicators in ensuring the sufficient disinfection effectiveness for various pathogenic viruses.

키워드

참고문헌

  1. Miura, T., Masago, Y., Chan, Y. M., Imai, T. and Omura, T., "Detection of bacteria and enteric viruses from river and estuarine sediment," J. Water Environ. Technol., 7, 307-315 (2009). https://doi.org/10.2965/jwet.2009.307
  2. Suzuki, Y. and Suwa, M., "Control and management of pathogens discharged to environment," Modern Media, 52, 352-358(2006).
  3. Albert, B., "Human enteric viruses in the water environment: a minireview," Internatl. Microbiol., 1, 191-196(1998).
  4. Skraber, S., Ogorzaly, L., Helmi, K., Maul, A., Hoffmann, L., Cauchie, H. M. and Gantzer C., "Occurrence and persistence of enteroviruses, noroviruses and F-specific RNA phages in natural wastewater biofilms," Water Res., 43, 4780-4789(2009). https://doi.org/10.1016/j.watres.2009.05.020
  5. Jeanette, A. T., Charles, N. H., Joseph, J., Kelley, R. and Charles, P. G., "Inactivation of Feline Calicivirus and Adenovirus Type 40 by UV Radiation," Appl. Environ. Microbiol., 69, 577-582(2003). https://doi.org/10.1128/AEM.69.1.577-582.2003
  6. Implementing the BEACH Act of 2000, U.S.EPA(2006).
  7. Water sanitation and health, Microbiological aspects (as of 2010/7/14), WHO, http://www.who.int/water_sanitation_health/dwq/dwqtraining/en/index.html
  8. Kamiko, N. and Ohgaki, S., "Multiplication characteristics of FRNA phage and utilization as an indicator for pathogenic virus," J. Japan Soc. Water Environ., 16, 723-731 (1993). https://doi.org/10.2965/jswe.16.723
  9. Tchobanoglous, G., Wastewater Engineering: Treatment and Reuse Metcalf and Eddy, Mc-Graw Hill, International Edition(2003).
  10. Jothikumar, N., Cromeans, T. L., Hill, V. R., Lu, X., Sobsey, M. D. and Erdman, D. D., "Quantitative Real-Time PCR Assays for Detection of Human Adenoviruses and Identification of Serotypes 40 and 41," Appl. Environ. Microbiol., 71, 3131-3136(2005). https://doi.org/10.1128/AEM.71.6.3131-3136.2005
  11. McQuaig, S. M., Scott, T. M., Lukasik, J. O., Paul, J. H. and Harwood, V. J., "Quantification of Human Polyomaviruses JC Virus and BK Virus by TaqMan Quantitative PCR and Comparison to Other Water Quality Indicators in Water and Fecal Samples," Appl. Environ. Microbiol., 75, 3379-3388(2009). https://doi.org/10.1128/AEM.02302-08
  12. Costán-Longares, A., Montemayor, M., Payán, A., Méndez, J., Jofre, J., Mujeriego, R. and Lucena, F., "Microbial indicators and pathogens: removal, relationships and predictive capabilities in water reclamation facilities," Water Res., 42, 4439-4448(2008). https://doi.org/10.1016/j.watres.2008.07.037
  13. 大瀧雅寛, 大腸菌ファージ測定法, 東大都市工版
  14. 上條絵美子, "下水処理水のオゾン消毒における大腸菌フ ァージの不活化速度," 東京大学新領域創成科学研究科環 境工学専攻修士論文(2004).
  15. Haveelar, A. H., "F-specific RNA bacteriophage as model viruses in UV disinfection of wastewater," Water Sci. Technol., 24, 347-352(1991).
  16. 하수도 유지관리지침, 일본하수도협회(2003).
  17. Cho, M., Chung, H., Choi, W. and Yoon, J., "Linear correlation between inactivation of E. coli and OH radical concentration in $TiO_2$ photocatalytic disinfection," Water Res., 38, 1069-1077(2004). https://doi.org/10.1016/j.watres.2003.10.029
  18. 자외선조사 -물의 소독에의 적용성-, 기보당출판(2008).
  19. Kott, Y., Ari, B. H. and Buras, N., "The fate of viruese in a marine environment", In Advances in Water Pollution Research: Proc. 4th Int. Conf. Prague., Pergamon Press, Oxford(1969).
  20. Metcalf, T. G., Vaughn, J. M. and Stiles, W. C., "The occurrence of human viruses and coliphages in marine waters and shellfish," FAO Tech. Conf. Marine Pollution and its Effects on Living Resources and Fishing, FAO, Rome(1970).
  21. Lee, S., Nakamura, M. and Ohgaki, S., "Inactivation of phage Q$\ss$ by 254 nm UV light and titanium dioxide photocatalyst," J. Environ. Sci. Health, Part A, 33, 1643-1655 (1998). https://doi.org/10.1080/10934529809376809
  22. Watts, R. J., Kong, S., Orr, M. P., Glenn, C. M. and Henry, B. H., "Photocatalytic inactivation of coliform bacteria and viruses in secondary wastewater," Water Res., 29, 95-100 (1995). https://doi.org/10.1016/0043-1354(94)E0122-M
  23. Cho, M, Chung, H., Choi, W. and Yoon J., "Linear correlation between inactivation of E. coli and OH radical concentration in $TiO_2$ photocatalytic disinfection," Water Res., 38, 1069-1077(2004). https://doi.org/10.1016/j.watres.2003.10.029
  24. Gehringer, P., Eschweiler, H., Leth, H., Pribil, W., Pfleger, S., Cabaj, A., Haider, T. and Sommer, R., "Bacteriophages as viral indicators for radiation processing of water: a chemical approach," Appl. Radiat. Isot., 58, 651-656(2003). https://doi.org/10.1016/S0969-8043(03)00089-7