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Applicability Investigation of E.coli, RNA and DNA Bacteriophages for Possible Indicator Microorganisms Based on the Inactivation Effectiveness by UV  

Kim, Il-Ho (Research Center for Environmental Quality Management, Graduate School of Engineering, Kyoto University)
Wahid, Marfiah AB (Research Center for Environmental Quality Management, Graduate School of Engineering, Kyoto University)
Tanaka, Hiroaki (Research Center for Environmental Quality Management, Graduate School of Engineering, Kyoto University)
Publication Information
Journal of Korean Society of Environmental Engineers / v.32, no.11, 2010 , pp. 1063-1068 More about this Journal
Abstract
This study compared UV and UV/$H_2O_2$ inactivation of E.coli, a possible indicator microorganism for fecal contamination of water, and $Q{\ss}$ phage, an indicator for pathogenic viruses. UV inactivation of $Q{\ss}$, T4 and lambda phages in actual secondary effluent was investigated, too. As a result, similar inactivation efficiency between $Q{\ss}$ phage and E.coli was observed during UV treatment, while $Q{\ss}$ phage showed higher resistance to UV/$H_2O_2$ than E.coli. $Q{\ss}$ phage resistance to UV or UV/$H_2O_2$ does not reflect those of all pathogenic viruses. However, the result tells that the use of E.coli inactivation efficiency in evaluating microbiological safety of water could not always ensure the sufficient safety from pathogenic viruses. Meanwhile, $Q{\ss}$ phage showed less resistance to UV than T4 and lambda phages, indicating that the use of $Q{\ss}$ phage as an indicator virus may bring insufficient disinfection effectiveness by causing the introduction of lower UV dose than required. Consequently, it can be thought that T4 or lambda phages would be more desirable indicators in ensuring the sufficient disinfection effectiveness for various pathogenic viruses.
Keywords
Pathogen; E.coli; Bacteriophage; UV; Water Reuse;
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