Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Effects of Plant-origin Biological Active Materials on the Activities of Pathogenic Microbes and Rumen Microbes

식물유래 생리활성물질의 병원성 미생물 및 반추위 미생물 활성에 대한 영향

  • Published : 2006.10.31
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Abstract

In order to know the effects of Garlic, Scallion, Flavonoid, Urushiol, Anthocyanidin and Bio-MOS?? on pathogenic microbes and rumen anaerobic microbes, the growth rate of pathogens (including Escherichia coli O157, Salmonella paratyphi, Listeria monocytogenes and Staphylococcus aureus) and in vitro rumen microbial growth, gas production, ammonia concentration, carboxymethylcellulase(CMCase) activity, and microbial populations were investigated.The growth of pathogens was inhibited by supplementation of 0.1% Flavonoid, Scallion or Bio-MOS?? as biological active materials. And Scallion and Flavonoid had powerful antimicrobial properties on the pathogens applied in paper disc method.Although few effects by biological active materials disappeared in rumen fermentation in vitro, CMCase activity removed with supplementation of 1% of Flavonoid which had antimicrobial property in paper disc method. Scallion, having powerful antimicrobial property on pathogens and no inhibiting on rumen fermentation, might be a source in development of natural antimicrobial agent for ruminants.

식물유래 생리활성물질인 garlic, scallion, fla- vonoid, urushiol 및 anthocyanidin과 기존의 항균 제품인 Bio-MOS?瑛? 병원성 미생물과 반추위 미생물에 대한 효과를 조사하기 위하여 병원성 균인 Escherichia coli O157, Salmonella paratyphi, Listeria monocytogenes, Staphylococcus aureus에 항균활성과 in vitro 반추위 미생물 성장률, 가스 생성량, 암모니아 농도, CMCase 활성 및 미생물 수를 측정하였다. 생리활성물질 0.10% 첨가 후, 배양시험에서는 flavonoid, scallion 및 Bio-MOS?怜? 병원성 미생물의 성장을 억제하였으나, paper disc법에 의한 항균활성 시험에서는 flavonoid나 scallion을 5.00%이상 처리 시 clear zone이 뚜렷하였으며, scallion이 가장 강력한 항균활성을 가지고 있는 것으로 나타났다. 생리활성물질의 in vitro 반추위 미생물 발효에 대한 영향은 크지 않았는데, 병원균에 활성을 나타내었던 flavonoid는 1.00% 처리 시 CMCase의 활성이 완전히 없어져 반추위 미생물에 대한 억제효과가 있는 것으로 나타났다. 그러나 scallion은 병원균에는 강한 항균활성을 가지면서 반추위 미생물 발효에는 저해작용을 나타내지 않아 반추 가축의 대체 천연항균제로서 개발 가능성을 나타내었다.

Keywords

References

  1. Abe, M., Shibui, H. and Kumeno, F. 1972. Improved method for counting rumen protozoa. Jap. J. Zootech. Sci. 43:535
  2. Akiko, S., Michiniri, T. and Miyoko, I. 1993. Antibacterial effect of garlic extract on Vibrio parahaemolyticus in fish meat. J. Food Hyg. Soc. Japan 34:63 https://doi.org/10.3358/shokueishi.34.63
  3. Ali, M, Thomson, M and Afzal, M. 2000. Garlic and onions: their effect on eicosanoid metabolism and its clinical relevance. Prostaglnadins. Leulotrienes and Essential Fatty Acids. 62:55 https://doi.org/10.1054/plef.1999.0124
  4. Bryant, M. P. and Robionson, I. M. 1961. An improved nonselective culture medium for ruminal bacteria and its use in determinig diurnal variation in numbers of bacteria in the rumen. J. Dairy Sci. 44:1446 https://doi.org/10.3168/jds.S0022-0302(61)89906-2
  5. Cavallito, C. J. and Bailey, J. H. 1944. Allicin, the antibacterial principle of Allium sativum. I. Isolation, physical properties, and antibacterial action. J. Am. Chem. Soc. 66:1950 https://doi.org/10.1021/ja01239a048
  6. Chaney, A. L. and Marbach, E. P. 1962. Modified reagents for determination of urea and ammonia. Clin. Chem. 8:130
  7. Chen, M. and Wolin, M. J. 1979. Effect of monensin and lasalocid-sodium on the growth of methanogenic and rumen saccharolytic bacteria. Appl. Environ. Microbial. 36:97
  8. Dehority, B. A. 1965. Degradation and utilization of isolated hemi-cellulose by pure cultures of cellulolytic rumen bacteria. J. Bacteriol. 89:1515
  9. El-shazly, K., Dehority, B. A. and Johnson, R R. 1961. Effect of starch on the digestion of cellulose in vitro and in vivo by rumen micro-organism. J. Anim. Sci. 20:268
  10. Ferorak, P. M. and Hrwdey, S. E. 1983. A simple apparatus for measuring gas production by methanogenic cultures in serum bottles. Environ. Technol. Lett. 4:268
  11. Holdman, L. V., Cato, E. P. and Moore, W. E. C. 1977. Anaerobic laboratory manual(4th ed.), Virginia Polutech. Inst. And Sate. Univ, Blackburg, Virginia
  12. Hong, J. H., Lee, M. H., Kang, M. C. and Hur, S. H. 2000. Separation and Identification of Antimicrobial Compounds from Korean Leek. J. Fd hgy. Safety. 15:235
  13. Hungate, R. E. 1966. The rumen and it's microbes. Academic press, NY
  14. Hungate, R. E. 1968. Ruminal fermentation. Handbook of physiology. Allimentary canal, Vol. V. pp.2725
  15. Kumar, M. and Berwal, J. S. 1998. Sensitivity of food pathogens to garlic. J. Apple. Microbiol. 84:213 https://doi.org/10.1046/j.1365-2672.1998.00327.x
  16. Lawson, L. D. 1991. Identification and HPLC quantitation of the sulphides and dia1k(en)yl thiosulphinates in commercial garlic products. Planta Med. 57:363 https://doi.org/10.1055/s-2006-960119
  17. Lowe, S. E., Theodorou, M. K., Trinci, A. P. J. and Hespell, R. B. 1985. Growth of an anaerobic rumen fungi on defind and semi-defind media lacking rumen fluid. J. Gen. Microbiol., 131, 2225
  18. Miller, G. L., Blum, R., Glennon, W. E. and Burton, A L. 1960. Measurement of carboxylmethylcellulase activity. Anal. Biochem. 1:127 https://doi.org/10.1016/0003-2697(60)90004-X
  19. Miller, W. C, Theilman, N. M., Swai, N., Cegielski, J. P., Shao, J., Ting, D., Mlalasi, J., Manyenga, D. and Lallinger, G. J. 1996. Delayed-type hypersensitivity testing in Tanzanian adults with mv infection. J. Acq. Immune Defic. Synd. Human Retrovir. 12:303 https://doi.org/10.1097/00042560-199607000-00012
  20. Morries, J. G., Baldwin, R. L., Maeng, W. J. and Maeda, B. T. 1975. Basic characteristics of a computer simulated model of nitrogen in the grazing ruminent. Tracer studies on non-protein nitrogen for ruminant. II. International Atomic Energy Agency. Vienn
  21. Mortensen, A, Skibsted, LH., Sampson, J., Rice-Evans, C. and Everett, SA. 1997. Comparative mechanisms and rates of free radical scavenging by carotenoid antioxidants. FEBS Lett. 418:91 https://doi.org/10.1016/S0014-5793(97)01355-0
  22. Ross, Z. M., O'Gara, E. A., Hill, D. J., Sleightolme, H V. and Maslin, D. J. 2001. Antimicrobial properties of garlic oil against human enteric bacteria: Evaluation of methodologies and comparisons with garlic oil sulfides and garlic powder. Appl. Environ. Microbiol. 67(1):475 https://doi.org/10.1128/AEM.67.1.475-480.2001
  23. Russell, J. B. 1987. A proposed mechanism of monensin action in inhibiting ruminal bacterial growth: Effect on ion flux and proton-motive force. J. Anim. Sci. 64:1519
  24. SAS. 1996 SAS Uaer Guide. Release 6.12 edition. SAS Inst. Inc. Cary NC. USA
  25. Seo, K. I., Moon, Y. H., Choi, S. U. and Park, K. H. 2001. Antibacterial activity of S-methyl methanethiosulfinate and S-methyl 2-propene-l-thiosulfinate from chinese chive toward Escherichia coli O157:H7. Biosci. Biotechnol. Biochem., 65(4): 966 https://doi.org/10.1271/bbb.65.966
  26. Sheo, H. J. 1999. The antibacterial action of garlic, onion, ginger and redpepper juice. J. Korean Soc. Food Sci. Nutr. 28:94
  27. Short, D. E. 1978. Rumen fermentation and nitrogen metabolism as effected by monensin. Ph. D. Thesis. University of Illinois, Urbana-Champaign
  28. Toshimitsu, H., Kazuko, S., Kawasaki, M., Munehisa, A., Shimizu, M. and Morita, N. 1988. Inhibition of cow's milk xantine oxidase by flavonoids. J. Natural Products. 51:345 https://doi.org/10.1021/np50056a030
  29. Wood, T. M., Wilson, C. A., McCrea, S. I. and Jobin, K. N. 1986. A highly active extracellular cellulase from the anaerobic rumen fungus Neocallimastix frontalis. FEMS. Microbiol. Lett. 43:37
  30. 박윤자, 김미향, 배송자. 2002. 부추 추출 성분의 항발암 효과연구. 한국식품과학회, 34(4):688
  31. 이성실, 하종규, 최윤재, 한인규, 문태현, 김창현. 1995. 반추위 곰팡이의 분리 동정과 섬유소 분해 효소의 특성 규명 빛 이들의 산업적 이용에 관 한 연구. II. 볏짚의 화학적 처리와 이의 급여가 축종별 반추위내 곰팡이의 군집변화에 미치는 영향. 한국영양사료학회지. 19:411
  32. 하종규, 이성실, 문양수, 김창현. 2005. 반추동물 영양생리학. 서울대학교출판부 서울, 대한민국