• Title/Summary/Keyword: 병원균 살균

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Chemical Resistance and Control of Dollar Spot Caused by Sclerotinia homoeocarpa on Turfgrass of Golf Courses in Korea (한국 골프장 잔디에서의 Sclerotinia homoeocarpa의 약제 저항성 및 방제)

  • Kim, Jeong-Ho;Choi, Hee-Youl;Shim, Gyu-Yul;Kim, Young-Ho
    • Asian Journal of Turfgrass Science
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    • v.24 no.2
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    • pp.170-175
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    • 2010
  • A total of 24 isolates of S. homoeocarpa were isolated from 16 golf courses in Korea. Chemical resistance of 24 isolates was determined by in vitro fungal growth on fungicide-amended media with thiophanatemethyl (Benzimidazole fungicide family), tebuconazole (demethylation inhibitor fungicide family: DMI), and iprodione (dicarboximide fungicide family). Results indicated that 83.3% of 24 isolates were resistant to iprodione, 62.5% resistant to thiophanate-methyl and 0% resistant to tebuconazale. The dual resistance of iprodione and thiophanate-methyl was 58.3.%. Occurrence rate of fungicide resistance of thiophanate-methyl and iprodione had no relation to turfgrass varieties and isolated locations of pathogen. In the filed test, procymidone, boscalid, and fluquinconazole+pyrimethanil effectively controlled the dollar spot of creeping bentgrass.

A Synthesis of New 2-Iminothiazolines and Their Antifungal Activities (II) (새로운 2-이미노티아졸린 유도체의 합성과 항균활성 (II))

  • Nam, Kee-Dal;Choi, Gyung-Ja;Cho, Kwang-Yun;Hahn, Hoh-Gyu
    • Applied Biological Chemistry
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    • v.41 no.6
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    • pp.471-476
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    • 1998
  • A synthesis and the screening of new 2-iminothiazolines (IV) of which structures are modified based on a lead compound, thiazoline for development of new agrochemical fungicide were described. Bromination of acetoacetanilides (I) which were prepared by the reaction of diketene with anilines gave the corresponding ${\gamma}-bromoacetoactanilide\;(II)$. Treatment of II with N-phenyl-N'-methyl thiourea (III) afforded IV, structure of which was confirmed by various spectroscopic methods. Antifungal activity of the new IV was tested against six kinds of typical plant diseases (in vivo). The IV with aromatic substituents showed remarkable activity against the Pyricuraria oryzae at 250 ppm in primary screening. The candidates with control value over 90% in primary screening were selected and further tested for second screening at lower concentrations. The IV which has an electron-withdrawing substituent such as halogen, especially fluorine in aryl group showed a higher activity as compared to those with electron-donating group and meta substituent was for optimal position.

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Foliar Application of the Fungicide Pyraclostrobin Reduced Bacterial Spot Disease of Pepper (Fungicide pyraclostrobin의 고추 세균점무늬병 예방효과)

  • Kang, Beom Ryong;Lee, Jang Hoon;Kim, Young Cheol
    • Research in Plant Disease
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    • v.24 no.1
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    • pp.59-65
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    • 2018
  • Pyraclostrobin is a broad-spectrum fungicide that inhibits mitochondrial respiration. However, it may also induce systemic resistance effective against bacterial and viral diseases. In this study, we evaluated whether pyraclostrobin enhanced resistance against the bacterial spot pathogen, Xanthomonas euvesicatora on pepper (Capsicum annuum). Although pyraclostrobin alone did not suppressed the in vitro growth of X. euvesicatoria, disease severity in pepper was significantly lower by 69% after treatments with pyraclostrobin alone. A combination of pyraclostrobin with streptomycin reduced disease by over 90% that of the control plants. The preventive control of the pyraclostrobin against bacterial spot was required application 1-3 days before pathogen inoculation. Our findings suggest that the fungicide pyraclostrobin can be used with a chemical pesticide to control bacterial leaf spot diseases in pepper.

Control Effect of Sodium Dichloroisocyanurate for Pear Scab (Venturia nashicola) on Niitaka Pear during Flowering Period (신고배 개화기 NaDCC 처리에 의한 검은별무늬병의 방제)

  • Nam, Ki-Woong;Han, Mi-Kyong;Yoon, Deok-Hoon
    • Korean Journal of Organic Agriculture
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    • v.22 no.2
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    • pp.347-357
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    • 2014
  • This study was conducted to evaluate the control effect of sodium dichloroisocyanurate (NaDCC) for Venturia nashicola on Niitaka pear during flowering period. As a nontoxic disinfectant, sodium dichloroisocyanurate is widely used in the field of hygiene and disease prevention, medical treatment, aquiculture as well as plant protection. NaDCC was sprayed on the pear tree inoculated with conidia ($4.5{\times}10^5spores/mL$) of Venturia nashicola and as a result the incidence of pear scab was 23.8% in 750mg/L and 26.2% in 1,000mg/L compare to the 51.6% incidence in untreated tree. No damage in the pollen of pear flower was detected with NaDCC treatments in the full bloom period for six pear cultivar including Wonwhang. A NaDCC single treatment in the early bloom of Niitaka pear showed more than 98% of fertilization rate. Furthermore, there was no incidence of Venturia nashicola on Nitaka pear trees treated with NaDCC 4 times during their growth period.

Inhibitory Effect of Flusilazole on the Spore Formation of Aspergillus niger Causing the Onion Black Mold in Vapour Phase (Flusilazole의 훈증 효과에 의한 양파검은곰팡이병균(Aspergillus niger)의 포자 형성 억제)

  • Kim, Heung-Tae;Park, Se-Won;Choi, Gyung-Ja;Kim, Jin-Cheol;Cho, Kwang-Yun
    • Research in Plant Disease
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    • v.8 no.2
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    • pp.124-130
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    • 2002
  • In 1998, a pathogen isolated from infected onions was identified as Aspergillus niger. At 3$0^{\circ}C$, A. niger AnYD-1 showed the best mycelial growth, spore germination, and high pathogenicity to onions. In spite of the weak inhibitory effect of flusilazole and hexaconazole on the mycelial growth on PDA, they showed the specific inhibitory activity against the formation of spores in the vapour phase. With flusilazole and hexaconazole, the effects of the solvent, the applied concentration and the incubating temperature on the activities inhibiting the spore formation were confirmed. Their inhibitory effect on the spore formation in vapour phase was excellent by solving them with dimethylsulfoxide and dimethylformamide among tested solvents, and applying them at high temperature such as 30~35$^{\circ}C$.

Identification of Pathogens Associated with Bulb Rot of Lily during Storage and Effects of Bulb Disinfection on Development of Lily Bulb Rot (백합 저장중 구근부패에 관여하는 병원균의 동정과 종구 소독효과)

  • Hahm Soo-Sang;Oh So-Young;Lee Eun-Mo;Yu Seung-Hun
    • Research in Plant Disease
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    • v.12 no.1
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    • pp.20-24
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    • 2006
  • Several pathogens associated with bulb rot of lilies in storage house were identified with respect to rot types. Rot patterns were grouped into four different types; brown rot of bottoms, brown rot of shoots, water-soaked rot and blue mold. Brown rot of bottoms was the highest in frequency with 72.5%, and brown rot of shoots the least with 23.0%. Dominant pathogens were differed with rot patterns, brown rot of bottoms by Fusarium oxysporum, blue mold and brown rot of shoots by Penicillium brevicompactum and P. fellutanum. In wound-inoculation tests, Penicillium and Fusarium isolates caused severe rot on the bulbs. Bulb disinfection before storage by captan showed the most prominent control value of 95.2% followed by thiophanate-methyl with 85.6%.

Morphological Changes of Fungal Cell Wall and ABC Transporter as Resistance Responses of Rice Bakanae Disease Pathogen Fusarium fujikuroi CF337 to Prochloraz (세포벽의 형태학적 변화와 ABC Transporter에 기초한 벼키다리병원균 Fusarium fujikuroi CF337의 살균제 prochloraz에 대한 저항성 반응)

  • Yang, You-Ri;Lee, Si-Woo;Lee, Se-Won;Kim, In-Seon
    • Korean Journal of Environmental Agriculture
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    • v.31 no.1
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    • pp.30-36
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    • 2012
  • BACKGROUND: The resistance of rice bakanae disease pathogens against the fungicide prochloraz has been reported. Understanding the resistance mechanisms is an important for better control of the pathogens. In the present study, we investigated the resistance mechanisms of Fusarium fujikuroi CF337 (CF337) against prochloraz. METHODS AND RESULTS: Morphological changes in the cell wall of CF337 grown in potato dextrose broth (PDB) with or without prochloraz was investigated by transmission electron microscopy. Growth inhibition of CF337 was examined in PDB containing prochloraz or an ABC transporter inhibitor or both of them. Cell wall thickness of CF337 grown in PDB with prochloraz was significantly increased from $80.73{\pm}1.99nm$ to $193.11{\pm}7.07nm$. Significant inhibition in the growth of CF337 was observed in the presence of both prochloraz and the inhibitor, but no growth inhibition was observed in the presence of the inhibitor or prochloraz. Sequence analysis of ATP-binding cassette transporter (ABC) gene of CF337 showed 70 to 80% similarities to the genes of the pathogens resistant to other fungicides. CONCLUSION: Efflux transporter system and changes in cell wall thickness were suggested as resistance mechanisms of CF337 against prochloraz.

Effect of Mixed Oxidants and Sodium Hypochlorite on Pathogenic Microorganisms in Olive flounder Paralichthys olivaceus Aquaculture on Jeju Island (제주도 양식 넙치(Paralichthys olivaceus)에서 분리한 병원균 3종에 대한 Mixed Oxidant 및 차아염소산나트륨 살균효과)

  • Park, Cheonman;Kim, Ki-hyuk;Moon, Hye-na;Yeo, In-Kyu
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.51 no.4
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    • pp.389-396
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    • 2018
  • Marine pathogenic bacteria, such as Streptococcus parauberis, Edwardsiella tarda and Vibrio harveyi, can cause lethal infections in farmed fish, ozone and antibiotics, are employed to sterilize waters used for rearing fish to mitigate this threat. The most widely used method is treatment with sodium hypochlorite solution. However, the maintenance of a constant concentration of chlorine in rearing waters can be difficult. We investigated the potential of a mixed oxidant (MO) solution generated by electrolysis of sea water to improve water quality. We measured the survival rates of fish pathogenic bacteria exposed to different concentrations of MO (0.5, 1.0, 1.5 and 2.0 MO) and sodium hypochlorite (0.5, 1.0, 1.5 and 2.0 ppm) for various lengths of time (0, 5, 10, 15, 20, 25 and 30 min). We found a time-dependent decrease in the survival rates of the tested pathogenic microorganisms. The sterilization effect of the MO solution on pathogenic organisms was greater than that of sodium hypochlorite for gram-negative and gram-positive bacteria. We conclude that MO solution produced by electrolysis could be used to maintain a constant chlorine concentration in aquaculture systems.

The Effectiveness of a New Systemic Fungicide EL-291 for the Control of Rice Blast Disease (새로운 침투성살균제 EL291의 벼 도열병 방제효과)

  • Hwang Byung Kook;Lee Eun Jong;Park Chang Seuk;Lee Kyung Hee
    • Korean journal of applied entomology
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    • v.15 no.2 s.27
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    • pp.57-60
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    • 1976
  • Experiments were carried out to determine the effectiveness of a new systemic fungicide EL-291 (5-Methyl-1, 2,4-triazolo (3,4-b) benzothiazole) for the control of rice blast disease in greenhouse and paddy field. The efficiency of EL-291 was much greater when applied before inoculation than when applied after inoculation. Kasugamin and Benlate were most effective as eradicants. For control of leaf blast, effectiveness of EL-291 was not significantly different than either Kasugamin or Benlate. However, EL-291 was considered more economical and reliable than either Kasugamin or Benlate. EL-291 required only a single foliar application or a transplant root soak, whereas two applications of the other fungicides were required. EL-191 was also more effective against panicle blast when applied only once, compared with two applications of Kasugamin or Benlate. The highest riceyields were obtained in plots treated with EL-29l.

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Biocontrol of Ginseng Damping-off by Bacillus velezensis CC112 (Bacillus velezensis CC112 균주의 인삼 잘록병에 대한 생물적 방제)

  • Lee, Sang Yeob;Song, Jaekyeong;Park, Kyeong Hun;Weon, Hang Yeon;Kim, Jeong Jun;Han, Ji Hee
    • The Korean Journal of Mycology
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    • v.44 no.3
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    • pp.176-183
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    • 2016
  • Bacillus velezensis CC112 inhibited the mycelial growth of several plant pathogens, including Rhizoctonia solani, causing damping-off on ginseng. The control efficacies of B. velezensis CC112 against R. solani by seed dipping in LB and BSM broth diluted 10 times, soil dipping, and soil drenching with LB broth diluted 10 times were 65.8%, 67.1%, and 64.2%, respectively. Treatment of soil drenching with the 100 times diluted prototype of B. velezensis CC112 against R. solani and Pythium sp. by soil revealed control efficacies of 77.3% and 65.7%, respectively. These results indicate that B. velezensis CC112 is a prospective biofungicide for the biological control of ginseng damping off.