The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Biocontrol of Ginseng Damping-off by Bacillus velezensis CC112

Bacillus velezensis CC112 균주의 인삼 잘록병에 대한 생물적 방제

  • Lee, Sang Yeob (Agricultural Microbiology Division, National Institute of Agricultural Sciences) ;
  • Song, Jaekyeong (Agricultural Microbiology Division, National Institute of Agricultural Sciences) ;
  • Park, Kyeong Hun (Mushroom Research Division, National Institute of Horticultural and Herbal Science) ;
  • Weon, Hang Yeon (Agricultural Microbiology Division, National Institute of Agricultural Sciences) ;
  • Kim, Jeong Jun (Agricultural Microbiology Division, National Institute of Agricultural Sciences) ;
  • Han, Ji Hee (Agricultural Microbiology Division, National Institute of Agricultural Sciences)
  • 이상엽 (국립농업과학원 농업미생물과) ;
  • 송재경 (국립농업과학원 농업미생물과) ;
  • 박경훈 (국립원예특작과학원 인삼과) ;
  • 원항연 (국립농업과학원 농업미생물과) ;
  • 김정준 (국립농업과학원 농업미생물과) ;
  • 한지희 (국립농업과학원 농업미생물과)
  • Received : 2016.09.12
  • Accepted : 2016.09.26
  • Published : 2016.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Bacillus velezensis CC112 inhibited the mycelial growth of several plant pathogens, including Rhizoctonia solani, causing damping-off on ginseng. The control efficacies of B. velezensis CC112 against R. solani by seed dipping in LB and BSM broth diluted 10 times, soil dipping, and soil drenching with LB broth diluted 10 times were 65.8%, 67.1%, and 64.2%, respectively. Treatment of soil drenching with the 100 times diluted prototype of B. velezensis CC112 against R. solani and Pythium sp. by soil revealed control efficacies of 77.3% and 65.7%, respectively. These results indicate that B. velezensis CC112 is a prospective biofungicide for the biological control of ginseng damping off.

Bacillusvelezensis CC112 균주는 인삼 잘록병을 일으키는 Rhizoctonia solani를 비롯한 여러 식물병원균의 균사 생장을 억제하였다. 인삼 잘록병균 R. solani에 대하여 B. velezensis CC112 균주를 Luria-Bertani (LB)와 Bacillus-soytone medium 배지에 배양한 10배 희석액의 종자침지처리와 LB 배지에 배양한 10배 희석액을 토양관주처리하여 65.8%, 67.1%와 64.2% 방제효과를 나타냈다. B.velezensis CC112 균주의 시제품을 100배로 희석하여 토양관주처리하여 R. solani에 77.3%, Pythium sp.에 65.7% 방제효과를 각각 나타내었다. 이들 결과에서 B. velezensis CC112 균주는 인삼 잘록병의 방제에 유망한 미생물살균제가 될 수 있다.

Keywords

References

  1. The Korean Society of Plant Pathology. List of plant diseases in Korea. 5th ed. Seoul: Korean Society of Plant Pathology; 2009.
  2. Rahman M, Punja ZK. Biological control of damping-off an American ginseng (Panax quinquefolius) by Clonostachys rosea f. catenulate (= Gliocladium catenulatum). Can J Plant Pathol 2007;29:203-7. https://doi.org/10.1080/07060660709507458
  3. Cho DH, Cho HS, Shin JS, Park DW. Control of damping-off and ginseng stem fungus gnat of ginseng. In: KT&G Ginseng re- search report. 2007. p. 28-33.
  4. Hong SK. Survey of ginseng cultivation in Southern Korea. So-yeun (Central Monopoly Institute) 1964;6:27-44.
  5. Yu YH, Cho DH, Ohh SH. Effect of tolclofos-methyl on damping- off of ginseng seedlings incited by Rhizoctonia solani. Korean J Ginseng Sci 1989;13:114-8.
  6. Georgakopoulos DG, Fiddaman P, Leifert C, Malathrakis NE. Biological control of cucumber and sugar beet damping off caused by Pythium ultimum with bacterial and fungal antagonists. J Appl Microbiol 2002;92:1078-86. https://doi.org/10.1046/j.1365-2672.2002.01658.x
  7. Howell CR, Stipanovic, RD. Suppression of Pythium ultimuminduced damping-off of cotton seedlings by Pseudomonas fluorescens and its antibiotic, pyoluteorin. Phytopathology 1980; 70:712-5. https://doi.org/10.1094/Phyto-70-712
  8. Zamanizadeh HR, Hatami N, Aminaee MM, Rakhshandehroo F. Application of biofungicides in control of damping disease off in greenhouse crops as a possible substitute to synthetic fungicides. Int J Environ Sci Technol (Tehran) 2011;8:129-36. https://doi.org/10.1007/BF03326202
  9. Kim JH, Choi YH, Kang SJ, Rhee IK, Joo GJ. Biocontrol of vegetables damping-off by Bacillus ehimensis YJ-37. Korean J Life Sci 2002;12:416-22. https://doi.org/10.5352/JLS.2002.12.4.416
  10. Joo GJ, Kim JH, Kang SJ. Isolation and antifungal activity of Bacillus ehimensis YJ-37 as antagonistic against vegetables damping- off fungi. Korean J Life Sci 2002;12:200-7. https://doi.org/10.5352/JLS.2002.12.2.200
  11. Yang HS, Sohn HB, Chung YR. Biological control of Pythium damping-off of cucumber by Bacillus stearothermophilus YC 4194. Res Plant Dis 2002;8:234-8. https://doi.org/10.5423/RPD.2002.8.4.234
  12. Jo EJ, Kang BG, Jang KS, Choi YH, Kim JC, Choi GJ. Control efficacy of Serenade formulation against Rhizoctonia and Pythium damping-off diseases. Res Plant Dis 2014;20:201-5. https://doi.org/10.5423/RPD.2014.20.3.201
  13. Lee SY, Kim BY, Ahn JH, Song J, Seol YJ, Kim WG, Weon HY. Draft genome sequence of the biocontrol bacterium Bacillus amyloliquefaciens strain M27. J Bacteriol 2012;194:6934-5. https://doi.org/10.1128/JB.01835-12
  14. Wang LT, Lee FL, Tai CJ, Kasai H. Comparison of gyrB gene sequences, 16s rRNA gene sequences and DNA-DNA hybridization in the Bacillus subtilis group. Int J Syst Evol Microbiol 2007;57:1846-50. https://doi.org/10.1099/ijs.0.64685-0
  15. Yamamoto S, Harayama S. PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains. Appl Environ Microbiol 1995;61:1104-9.
  16. Song J, Lee SC, Kang JW, Baek HJ, Suh JW. Phylogenetic analysis of Streptomyces spp. isolated from potato scab lesions in Korea on the basis of 16s rRNA gene and 16s-23s rDNA internally transcribed spacer sequences. Int J Syst Evol Microbiol 2004;54:203-9. https://doi.org/10.1099/ijs.0.02624-0
  17. Lee SY, Kim BY, Ahn JH, Song J, Seol YJ, Kim WG, Weon HY. Draft genome sequence of the biocontrol bacterium Bacillus amyloliquefaciens strain M27. J Bacteriol 2012;194:6934-5. https://doi.org/10.1128/JB.01835-12
  18. Wang LT, Lee FL, Tai CJ, Kasai H. Comparison of gyrB gene sequences, 16s rRNA gene sequences and DNA-DNA hybridization in the Bacillus subtilis group. Int J Syst Evol Microbiol 2007;57:1846-50. https://doi.org/10.1099/ijs.0.64685-0
  19. Yamamoto S, Harayama S. PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains. Appl Environ Microbiol 1995;61:1104-9.
  20. Song J, Lee SC, Kang JW, Baek HJ, Suh JW. Phylogenetic analysis of Streptomyces spp. isolated from potato scab lesions in Korea on the basis of 16s rRNA gene and 16s-23s rDNA internally transcribed spacer sequences. Int J Syst Evol Microbiol 2004;54:203-9. https://doi.org/10.1099/ijs.0.02624-0
  21. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62:716-21. https://doi.org/10.1099/ijs.0.038075-0
  22. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731-9. https://doi.org/10.1093/molbev/msr121
  23. Dunlap CA, Kim SJ, Kwon SW, Rooney AP. Bacillus velezensis is not a later heterotypic synonym of Bacillus amyloliquefaciens; Bacillus methylotrophicus, Bacillus amyloliquefaciens subsp. plantarum and 'Bacillus oryzicola' are later heterotypic synonyms of Bacillus velezensis based on phylogenomics. Int J Syst Evol Microbiol 2016;66:1212-7. https://doi.org/10.1099/ijsem.0.000858