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http://dx.doi.org/10.4489/KJM.2016.44.3.176

Biocontrol of Ginseng Damping-off by Bacillus velezensis CC112  

Lee, Sang Yeob (Agricultural Microbiology Division, National Institute of Agricultural Sciences)
Song, Jaekyeong (Agricultural Microbiology Division, National Institute of Agricultural Sciences)
Park, Kyeong Hun (Mushroom Research Division, National Institute of Horticultural and Herbal Science)
Weon, Hang Yeon (Agricultural Microbiology Division, National Institute of Agricultural Sciences)
Kim, Jeong Jun (Agricultural Microbiology Division, National Institute of Agricultural Sciences)
Han, Ji Hee (Agricultural Microbiology Division, National Institute of Agricultural Sciences)
Publication Information
The Korean Journal of Mycology / v.44, no.3, 2016 , pp. 176-183 More about this Journal
Abstract
Bacillus velezensis CC112 inhibited the mycelial growth of several plant pathogens, including Rhizoctonia solani, causing damping-off on ginseng. The control efficacies of B. velezensis CC112 against R. solani by seed dipping in LB and BSM broth diluted 10 times, soil dipping, and soil drenching with LB broth diluted 10 times were 65.8%, 67.1%, and 64.2%, respectively. Treatment of soil drenching with the 100 times diluted prototype of B. velezensis CC112 against R. solani and Pythium sp. by soil revealed control efficacies of 77.3% and 65.7%, respectively. These results indicate that B. velezensis CC112 is a prospective biofungicide for the biological control of ginseng damping off.
Keywords
Antifungal activity; Bacillus velezensis; Biological control; Damping-off; Ginseng;
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1 Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62:716-21.   DOI
2 Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731-9.   DOI
3 Dunlap CA, Kim SJ, Kwon SW, Rooney AP. Bacillus velezensis is not a later heterotypic synonym of Bacillus amyloliquefaciens; Bacillus methylotrophicus, Bacillus amyloliquefaciens subsp. plantarum and 'Bacillus oryzicola' are later heterotypic synonyms of Bacillus velezensis based on phylogenomics. Int J Syst Evol Microbiol 2016;66:1212-7.   DOI
4 The Korean Society of Plant Pathology. List of plant diseases in Korea. 5th ed. Seoul: Korean Society of Plant Pathology; 2009.
5 Howell CR, Stipanovic, RD. Suppression of Pythium ultimuminduced damping-off of cotton seedlings by Pseudomonas fluorescens and its antibiotic, pyoluteorin. Phytopathology 1980; 70:712-5.   DOI
6 Rahman M, Punja ZK. Biological control of damping-off an American ginseng (Panax quinquefolius) by Clonostachys rosea f. catenulate (= Gliocladium catenulatum). Can J Plant Pathol 2007;29:203-7.   DOI
7 Cho DH, Cho HS, Shin JS, Park DW. Control of damping-off and ginseng stem fungus gnat of ginseng. In: KT&G Ginseng re- search report. 2007. p. 28-33.
8 Hong SK. Survey of ginseng cultivation in Southern Korea. So-yeun (Central Monopoly Institute) 1964;6:27-44.
9 Yu YH, Cho DH, Ohh SH. Effect of tolclofos-methyl on damping- off of ginseng seedlings incited by Rhizoctonia solani. Korean J Ginseng Sci 1989;13:114-8.
10 Georgakopoulos DG, Fiddaman P, Leifert C, Malathrakis NE. Biological control of cucumber and sugar beet damping off caused by Pythium ultimum with bacterial and fungal antagonists. J Appl Microbiol 2002;92:1078-86.   DOI
11 Zamanizadeh HR, Hatami N, Aminaee MM, Rakhshandehroo F. Application of biofungicides in control of damping disease off in greenhouse crops as a possible substitute to synthetic fungicides. Int J Environ Sci Technol (Tehran) 2011;8:129-36.   DOI
12 Kim JH, Choi YH, Kang SJ, Rhee IK, Joo GJ. Biocontrol of vegetables damping-off by Bacillus ehimensis YJ-37. Korean J Life Sci 2002;12:416-22.   DOI
13 Joo GJ, Kim JH, Kang SJ. Isolation and antifungal activity of Bacillus ehimensis YJ-37 as antagonistic against vegetables damping- off fungi. Korean J Life Sci 2002;12:200-7.   DOI
14 Yang HS, Sohn HB, Chung YR. Biological control of Pythium damping-off of cucumber by Bacillus stearothermophilus YC 4194. Res Plant Dis 2002;8:234-8.   DOI
15 Lee SY, Kim BY, Ahn JH, Song J, Seol YJ, Kim WG, Weon HY. Draft genome sequence of the biocontrol bacterium Bacillus amyloliquefaciens strain M27. J Bacteriol 2012;194:6934-5.   DOI
16 Jo EJ, Kang BG, Jang KS, Choi YH, Kim JC, Choi GJ. Control efficacy of Serenade formulation against Rhizoctonia and Pythium damping-off diseases. Res Plant Dis 2014;20:201-5.   DOI
17 Lee SY, Kim BY, Ahn JH, Song J, Seol YJ, Kim WG, Weon HY. Draft genome sequence of the biocontrol bacterium Bacillus amyloliquefaciens strain M27. J Bacteriol 2012;194:6934-5.   DOI
18 Wang LT, Lee FL, Tai CJ, Kasai H. Comparison of gyrB gene sequences, 16s rRNA gene sequences and DNA-DNA hybridization in the Bacillus subtilis group. Int J Syst Evol Microbiol 2007;57:1846-50.   DOI
19 Yamamoto S, Harayama S. PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains. Appl Environ Microbiol 1995;61:1104-9.
20 Song J, Lee SC, Kang JW, Baek HJ, Suh JW. Phylogenetic analysis of Streptomyces spp. isolated from potato scab lesions in Korea on the basis of 16s rRNA gene and 16s-23s rDNA internally transcribed spacer sequences. Int J Syst Evol Microbiol 2004;54:203-9.   DOI
21 Wang LT, Lee FL, Tai CJ, Kasai H. Comparison of gyrB gene sequences, 16s rRNA gene sequences and DNA-DNA hybridization in the Bacillus subtilis group. Int J Syst Evol Microbiol 2007;57:1846-50.   DOI
22 Yamamoto S, Harayama S. PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains. Appl Environ Microbiol 1995;61:1104-9.
23 Song J, Lee SC, Kang JW, Baek HJ, Suh JW. Phylogenetic analysis of Streptomyces spp. isolated from potato scab lesions in Korea on the basis of 16s rRNA gene and 16s-23s rDNA internally transcribed spacer sequences. Int J Syst Evol Microbiol 2004;54:203-9.   DOI