• Korean Chemical Engineering Research
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• v.43 no.2
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• pp.187-201
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• 2005

Bioprocessing technologies utilizing 'biorecognition' between a solid matrix and a protein is being widely experimented as a means to replacing the conventional, solution-based technology. Frequently the matrices are chromatographic resins with specific functional groups exposed outside. Since the reactions of and interactions with the proteins occur as they are attached to the solid matrix, this 'solid-phase' processing has distinct advantages over the solution-phase technology. Solid-phase refolding of inclusion body proteins uses ion exchange resins to adsorb denaturant-dissolved inclusion body. As the denaturant is slowly removed from the micromoiety around the protein, it is refolded into a native, three-dimensional structure. Once the refolding is complete, the folded protein can be eluted by a conventional elution technique such as the salt-gradient. This concept was successfully extended to 'EBA (expanded bed adsorption)-mediated refolding,' in which the denaturant-dissolved inclusion body in whole cell homogenate is adsorbed to a Streamline resin while cell debris and other impurity proteins are removed by the EBA action. The adsorbed protein follows the same refolding steps. This solid-phase refolding process shows the potential to improve the refolding yield, reduce the number of processing steps and the processing volume and time, and thus improve the overall process economics significantly. In this paper, the experimental results of the solid-phase refolding technology applied to several biopharmaceutical proteins of various types are presented.

• Economic and Environmental Geology
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• v.52 no.5
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• pp.347-356
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• 2019

Various studies regarding the sedimentary environment, depositional age, provenance, and metamorphic history have been carried out on the Taean Formation in the western part of Gyeonggi Massif, since the unique detrital zircon age pattern was revealed. This review paper introduces the previous researches on the Taean Formation and discusses the depositional age and provenance. The Taean Formation was traditionally regarded as a Precambrian stratigraphic unit, but recently it is interpreted to be a middle or upper Paleozoic formation due to the occurrence of large amounts of Early to Middle Paleozoic detrital zircons. The Taean Formation consists of metasandstone, argillaceous schist, and phyllite which are mainly made up of quartz and mica. The protoliths are interpreted as turbidites deposited in deep sea fan environment. The Taean Formation has been interpreted to be deposited between the Devonian to Triassic ages given the age differences between detrital zircons and intrusive rocks. There are two opinions that the deposition age is close to the Devonian or the Permian period. The provenance of this formation is supposed to be South China block, Chinese collisional belt, or Gyeonggi Massif. Given the available detrital zircon ages of the Taean Formation and other Korean (meta)sedimentary rocks, the Taean Formation shares major source rocks with Yeoncheon Group and Pibanryeong Unit of the Okcheon Supergroup, but their source regions are not entirely consistent. Considering the existing hypotheses about the depositional timing and provenance, we put weight on the possibility that the Taean Formation was deposited between Permian and Early Triassic periods. However, further studies on the stratigraphy and sedimentary petrology are needed to clarify its definition and to elucidate the provenance.

• Korean Journal of Mineralogy and Petrology
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• v.33 no.4
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• pp.339-347
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• 2020

We carried out the U-Pb age dating of zircon from basement rocks in the southern part of the Danyang National Geopark. Two migmatitic gneisses composed of biotite±sillimanite±garnet+feldspar+quartz were dated. Leucosomes in the samples were clearly distinguished from their melanosomes. The U-Pb isotopic compositions of zircon from sillimanite- and garnet-bearing migmatitic samples were measured using a secondary ion microprobe, yielding metamorphic ages, 1870±10 Ma (2σ)와 1863±6 Ma (2σ), respectively. 1.87~1.86 Ga metamorphic ages are consistent with those of the Paleoproterozoic low-P and high-T regional metamorphism (1.87~1.85 Ga) in the Yeongnam Massif. The maximum depositional age based upon the apparent 207Pb/206Pb ages of detrital zircon in the samples was estimated as 2.06 Ga, and thus sedimentation age of the protolith of the migmatitic gneisses ranges between 2.06 and 1.87 Ga.

• Journal of Chest Surgery
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• v.31 no.11
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• pp.1023-1030
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• 1998

Background: Calcific degeneration limits durabilities of the bioprosthetic tissues implanted in the human body. The direct coupling sulphonated polyethyleneoxide(PEO-SO3) to the bioprosthetic tissues after glutaraldehyde(GA) fixation and the removal of residual aldehyde groups from the tissues can augment the effect of calcification-resistance. Materials and methods: To study the anti-calcification effect by PEO-SO3 modification and the removal of the residual aldehyde groups of tissues, surface modified bovine pericardia(BP-PEO-SO3) were preserved in aseptic saline to wash out GA(saline group) and 0.65% GA solution(GA group). And then above two groups and PERIGUARD (Bio-vascular. Co.) (product group) were evaluated with respects to calcium contents and microscopic findings using in vivo implantation models at carotid and femoral artery and peritoneum of 8 adult dogs. Results: In the tissues retrieved from carotid artery, calcium content was significantly decreased in saline group than in other two groups(saline; 2.89±0.31 vs. GA; 6.14±1.08 vs. product; 22.82±5.00 mg/g of dried tissue; p<0.05). In the tissues retrieved from femoral artery and peritoneum, calcium amount was also decreased in saline group than in other two groups, but not reached the significant difference between groups. On the other hand, the pathologic findings of pericardial tissues showed marked destructuction in GA group compared to the other two groups. Conclusions: In this study, covalently PEO-SO3 bound to bovine pericardium decreased calcifications and the anti-calcification effect of BP-PEO-SO3 could be augmented by the washing out the residual aldehyde groups using saline after GA fixation. Conclusively, the PEO-SO3 modified bovine pericardium is highly resistant to calcification and can be useful for the development of calcification-resistant cardiovascular patches and valves.

• The Journal of the Petrological Society of Korea
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• v.6 no.3
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• pp.244-244
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• 1997

The Janggunbong area(this study area) at the central-south part in the North Sobaegsan Massif, Korea, consists mainly of Precambrian(Wonnam and Yulri Formations)-Paleozoic [Joseon Supergroup(Jangsan Quarzite, Dueumri Formation and Janggum Limestone) and Pyeongan Group(Jaesan and Dongsugok Formations)] metasedimentary rocks and Mesozoic granitoid(Chunyang granite.) This study is to interpret geological structure of the North Sobaegsan Massif in the Jang-gunbong area by analysing rock-structure and microstructure of the constituent rocks. It indicates that its geological structure was formed at least by four phases of deformation after the formation of gneissosity(S0) in the Wonnam Formation and bedding plane(S0) in the Paleozoic metasedimentary rocks. The first phase deformation(D1) formed tight isoclinal fold(F1). Its axial plane(S1) strikes east-west and steeply dips north. Its axis (L1) subhorizontally plunges east-west. The second phase deformation(D2), which was related to ductile shear deformation, formed stretching lineation(L2) and shear foliation(S2). The sense of the shear movement indicates dextral strike-slip shearing(top-to-the east shearing). The third phase deformation(D3) formed open inclined fold(F3). Its axial plane(S3) strikes east-west and moderately or gently dips north. Its axis(L3) subhorizontally plunges east-west. The F3 fold reoriented the original north-dipping S1 foliation and D2 shear sense into south-dipping S1 foliation(top-to-the west shear sense on this foliation) at its a limb. The four phase of deformation(D4) formed asymmetric-type open inclined fold(F4) of NE-vergence with NW striking axial plane(S4) and NW-NNW plunging axis(L4). The F4 fold partly reoriented pre-D4 structural elements with east-west trend into those with north-south trend. Such reorientation is recognized mainly in the Paleozoic metasedimentary rocks.

• The Journal of the Petrological Society of Korea
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• v.6 no.3
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• pp.224-259
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• 1997

The Janggunbong area(this study area) at the central-south part in the North Sobaegsan Massif, Korea, consists mainly of Precambrian(Wonnam and Yulri Formations)-Paleozoic [Joseon Supergroupuangsan Quarzite, Dueumri Formation and Janggun Limestone) and Pyeongan Group (Jaesan and Dongsugok Formations)l metasedimentary rocks and Mesozoic granitoid(Chunyang granite). This study is to interpret geological structure of the North Sobaegsan Massif in the Janggunbong area by analysing rock-structure and microstructure of the constituent rocks. It indicates that its geological structure was formed at least by four phases of deformation after the formation of gneissosity(S0) in the Wonnam Formation and bedding plane(S0) in the Paleozoic metasedimentary rocks. The first phase deformation(D1) formed tight isoclinal fold(F1). Its axial plane(S1) strikes east-west and steeply dips north. Its axis(L1) subhorizontally plunges east-west. The second phase deformation(D2), which was related to ductile shear deformation, formed stretching lineation(L2) and shear foliation(S2). The sense of the shear movement indicates dextral strike-slip shearing(topto-the east shearing). The third phase deformation(D3) formed open inclined fold(F3). Its axial plane(S3) strikes east-west and moderately or gently dips north. Its axis(L3) subhorizontally plunges east-west. The F3 fold reoriented the original north-dipping S1 foliation and D2 shear sense into south-dipping S1 foliation(top-to-the west shear sense on this foliation) at its a limb. The four phase of deformation(D4) formed asymmetric-type open inclined fold(F4) of NE-vergence with NW striking axial plane(%) and NW-NNW plunging axis(L4). The F4 fold partly reoriented pre-D4 structural elements with east-west trend into those with north-south trend. Such reorientaion is recognized mainly in the Paleozoic metasedimentary rocks.

• Korean Journal of Food Science and Technology
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• v.30 no.4
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• pp.862-870
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• 1998

This study shows the application of Ci-ELISA method for monitoring the denaturation of myosin by the frozen treatment in order to differentiate thawed beef from chilled. Hanwoo M.Semitendinosus (n=25) was treated under the two different frozen process as follows; simple frozen treatment (Exp-1) at 4 different temperatures, -10, -20, -50 and $-80^{\circ}C$, respectively, and repeated thawing-refreezing treatment (Exp-2) stored at 4 different temperatures, -10, -20, -50 and $-80^{\circ}C$, respectively. Antibodies (Abs) were produced from rabbits immunized with myosin whole molecule (MWM) isolated from beef round, heavy meromyosin S-1 (S-1) and light meromyosin (LMM) prepared by digestion of MWM. Each immunoglobulin G (IgG) was separated from antiserum. At 6 month storage, IA of anti-MWM IgG for myosin was decreased to 32.67, 32. 23, 51.52 and 34.27% in Exp-1 and to 14.82, 15.61, 25.3 and 23.7% in Exp-2 at -10, -20, -50 and $-80^{\circ}C$, respectively (P<0.05). In Exp-1, the reactivities of anti-LMM IgG were decreased to 25.12, 21.42, 49.05 and 28.96%, and those of Exp-2 were to 11.88, 9.56, 20.63 and 12.64% at -10, -20, -50 and $-80^{\circ}C$, respectively, at 6 times thawing (P<0.05). Conclusively, myosin was denaturated by freezing treatment and LMM or myosin rod part might have suffered from more extreme demage than HMM S-1, and samples at $-50^{\circ}C$ were slightly injured less than others by freezing treatment.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.57 no.6
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• pp.1017-1021
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• 2008

A calculable potential transformer(PT) with nominal ratio error in wide range of -10% to +10% has been developed on basis of theoretical calculation of ratio error by the number of windings. The developed PT can be used to evaluate the linearity and accuracy of the PT comparator by comparing both the theoretical and experimental values of the PT which have exactly same ratio errors in nominal and calculated values. The PT has been applied for calibration and correction of the PT comparator up to wide ratio error range of -10% to +10%. This portable PT is very convenient to carry to the power industry for the on-site calibration of the PT comparator.

• Proceedings of the Korean Information Science Society Conference
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• 2006.06d
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• pp.22-24
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• 2006

유선 환경의 초고속 인터넷 서비스는 일반적으로 PC와 같은 고정형 단말을 대상으로 한다. 휴대폰이나 네비게이션용 GPS 단말 같은 이동 단말은 비교적 정확하게 단말의 위치를 찾을 수 있어 이를 활용한 응용 서비스가 확대되는 추세이다. 유선 인터넷 단말의 경우 IP주소의 할당 내역을 바탕으로 비교적 넓은 영역의 지역 정보를 찾아서 이용하는 Geo Targeting이 미국 등 해외에서 활성화 되고 있으나 국내에서는 IP에 대한 지역정보를 얻을 수 있는 적절한 방법이 없어 관련 서비스가 크게 활성화되고 있지 못하다. 본 논문에서는 유선 인터넷 환경 하의 Geo Targeting 구현에 대해 설명하고 이를 활용한 응용 서비스를 소개한다.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2009.11a
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• pp.232-232
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• 2009

In this paper, we investigated the sensitivity characteristics of $Bi_{12}BiO_{20}$ (BSO) voltage transformer (VT) by utilizing optical output measured according to the variation of ambient temperature and applied voltage. Fabricated BSO VT slightly showed the decrease of the accuracy in range of from $-20^{\circ}C$ to $50^{\circ}C$, on the other hand, the variation of the optical output result was not observed at the variation of applied voltage. We could finally confirm the temperature stability, applied voltage range, and the possibility that BSO could be applied for optical sensors in GIS system.