• Korean Journal of Environmental Biology
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• v.29 no.4
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• pp.241-250
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• 2011

The safety of human exposure to an ever-increasing number and diversity of electromagnetic field (EMF) sources both at work and at home has clearly become a public health issue. To date many $in$ $vivo$ and $in$ $vitro$ studies revealed that EMF exposure can alter cellular metabolism, endocrine function, immune activity, reproductive function, and fetal development in animal system. The major parameters found to be altered in cells or individuals following EMF exposure include an increase of free radicals, DNA damage, cancer risk, developmental defect, and reproductive dysfunctions. Epidemiological studies reported EMF can increase life-threatening illnesses such as leukemia, brain cancer, amyotrophic lateral sclerosis, clinical depression, suicide, and Alzheimer's disease has been identified. These effects of EMF exposure differ according to duration of exposure, frequency of waves, and strength (energy) of EMF. In the present review, we briefly introduced the physical properties of EMF and summarized the effect of EMF on human and wildlife animals according to types of EMF, duration of exposure at cellular and organism levels.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2002.11a
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• pp.114-114
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• 2002

포유동물에서 뇌와 부신에서 합성.분비되는 카테콜아민(Catecholamine, CA)계 신경전달물질인 dopamine(DA), norepinephrine(NE), epinephrine(E)은 체내 각종 생리현상의 조절에 필수적이며, 생식과 관련지어서는 시상하부-뇌하수체 간 GnRH-gonadotropin 호르몬 축의 활성을 조절하는 기능 외에도 번식과 관련된 여러 행동양식을 조절함이 잘 알려져 있다. 본 연구는 CA 생합성 효소들인 tyrosine hydroxylase(TH), dopamine beta-hydroxylase(DBH), phenylethanolamine-N-methyltransferase(PNMT)의 유전자 발현에 미치는 sex steroid의 영향을 조사하였다. 성숙한 암컷 횐쥐(SD strain)의 난소를 제거하고 1주 경과 후 vehicle(sesame oil; OVX+Oil 실험군) 또는 estradiol 17$\beta$(235ug/m1; OVX+E$_2$실험군)이 든 silastic capsule(길이 14mm; 내경 1.55mm; 외경 3.125mm)을 48 시간 동안 처리한 뒤 희생시켰다. 적출된 조직으로부터 RNA를 추출한 후 semi-quantitative RT-PCR을 시행하였다. (i) TH의 발현 정도는 OVX+Oil 군에서는 시상하부) substantia nigra(SNc)) 부신 순으로, OVX+E$_2$군에서는 SN글 부신) 시상하부 순으로 나타났다. TH 발현에 미치는 estradiol의 효과로 SNc과 부신에서는 유의한 증가를 보인데 비해 시상하부에서는 유의한 감소를 관찰하였다. (ii) DBH 발현 정도는 OVX+Oil군에서는 SNc> 부신> 시상 하부 순으로, OVX+E$_2$군에서는 부신＞ SNc＞ 시상하부 순이었다. DBH 발현에 미치는 estradiol의 효과로 SNc에서는 유의한 감소, 부신에서는 유의한 증가, 그리고 시상하부에서는 통계적 유의성은 없으나 감소하는 경향을 보였다. (iii) PNMT의 발현의 경우 SNc와 시상하부에서는 기보고된 바와 같이 alternative splicing에 의해 110bp 차이의 크고 작은 두 형태의 cDNA(PNMTI & PNMTs)가 증폭되었으나 부신에서는 작은 cDNA 만이 관찰되었다. PNMTs의 발현 정도는 OVX+Oil군과 OVX+E$_2$군에서 공히 부신＞ 시상하부＞ SNc 순이었고, PNMTI의 발현은 SNc가 시상하부 보다 다소 높은 경향이었으나 유의성은 없었다. PNMTs 발현에 미치는 estradiol의 효과로 SNc에서는 유의한 감소, 부신에서는 유의한 증가, 그리고 시상하부에서는 통계적 유의성은 없으나 증가하는 경향을 보였다. 본 연구에서는 CA 생합성 효소들의 유전자 발현의 조절에 미치는 estrogen 의 영향이 세포 기원이 neural crest cell인 부신 수질은 물론 뇌의 상이한 지역간에서도 조직특이적임을 관찰하였다. 이러한 결과는 각 조직에서의 estrogen 수용체 유형의 차이 혹은 작용 모드와 각 효소 유전자 발현 사이에 중요한 상관관계가 있음을 시사한다.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.23 no.1
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• pp.97-106
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• 2020

Urban parks provide a variety of ecosystem services and are an important means of providing positive functions to urban ecosystems. Recently, various studies on wildlifes in urban parks have been conducted. However, there is a lack of research on habitat use in urban parks at important times such as before and after fledging in bird ecology. This study analyzed habitat use and home-range before and after fledging on Cheongsa park, a neighborhood park located in Cheonan city. An artificial nest was set up to check and capture great tit in fledging time. One female was captured and attached to the NTQB-2 (0.4g) radio transmitter, the location was tracked using SIKA Radio Tracking Receiver, hand-held three element Yagi antenna and GPS. Location information was recorded for 10 minutes for 3 hours each morning and afternoon for 12 days from May 17 to May 31, 2019. As a result, the home-range of the target species was 1.776 ha (MCP) and the core area was 499 ㎡ (KD 50%). The average daily home-range was 0.513 ha for the entire period, 0.688 ha before fledging, 0.339 ha after fledging based on MCP. The bird moved about 29.9 m on average and moved up to 131.7 m. For the most of the time, the great tit stayed inside the park, but the bird also used small green spaces such as street trees, tree flower beds, and green areas of unused lands. The results of this study could be applied to the study of habitat use and the greenery management policy of the urban park considering wild birds.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.23 no.4
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• pp.97-110
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• 2020

This study conducted observations at 72 traffic lights and road signs in Sejong-si, Korea from March to June 2019 in order to analyze nest-site, nest-site characteristics and feeding behavior in Tree sparrow (Passeer montanus) in urban. Feeding behavior during breeding season in 2019 was investigated at 10 Town representing traffic facilities installed on the road located in the center of the city and 10 Riverside Geumgang river's riverside road. We fitted both general linear model and regression model to our field data and estimated the spatial variation of initial date of the first feeding (i.e., being date of breeding) and the total number of caterpillar fed by parents per hour. Our results showed that the frequency of nest building on artificial structures, such as traffic lights, was about 26% higher and about 8 days earlier in the riverside. In particular, the first feeding was significantly faster when the nearest distance between nest to green field as foraging site was shorter whereas there was no significant effect of the number of nest within 50 meters on the first feeding. Number of caterpillars fed by parents was higher in the Riverside than in the Urban, and especially the number in the second brood was sharply decreased in the Urban. As we estimated average amount of caterpillars per hour to 446 traffic safety facilities in Sejong-si, it was estimated that Tree sparrows fed 18,742.8 caterpillars per hour during the breeding season. In this study, we showed that characteristics of nests built in artificial structures and feeding behavior in order to analyze effects of environmental condition in urban on life-history traits during the breeding season. Finally, we discussed the conservation of birds in urban and pest control effects.

• Ocean and Polar Research
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• v.41 no.3
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• pp.147-157
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• 2019

Our study was conducted to examine differences in incubation behavior among breeding sites and the relationship between factor affecting environmental change and incubation behavior of the Black-faced Spoonbill (Platalea minor). We set up the remote sensor cameras at three breeding sites (Mae-do, Namdongji, Guji-do) to observe incubation behavior in Gyeonggi Bay, South Korea from 2015 to 2018. We analyzed effects of breeding year, day of incubation started, day of incubation, the time of incubation exchanges and sex on incubation bout length. Mean incubation bout length of females (Mae-do: $7.19{\pm}0.23$ hours, Namdongji: $6.08{\pm}0.23$ hours, Guji-do: $7.96{\pm}0.30$ hours) was longer than males (Mae-do: $6.14{\pm}0.21$ hours, Namdongji: $5.45{\pm}0.28$ hours, Guji-do: $7.38{\pm}0.29$ hours). Mean incubation bout length was longer in Guji-do than other study sites. Incubation bout length tended to increase with the clutch initiation date. Males incubated their eggs at day time while female did at night time, these tendencies were observed more clearly in Guji-do. The proportion of time spent incubating of females was higher than males. Males' proportion increased as incubation progressed and increased rate in Guji-do was higher than other study sites. Our results showed that incubation rhythm of the Black-faced Spoonbill differed among breeding sites and varied with the environmental cycle.

• Korean journal of applied entomology
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• v.61 no.1
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• pp.15-28
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• 2022

Neuropeptides produced in neurosecretory cells are the largest group of insect hormones. They regulate various physiological functions, such as fat body homeostasis, feeding, digestion, excretion, circulation, reproduction, metamorphosis, and behavior throughout all life stages. The PRXamide peptide family (X, a variable amino acid) is a well-characterized neuropeptide component with a common amino acid sequence, PRXamide (NH₂), at the C-terminal end conserved across Insecta. The PRXamide peptides are classified into three subfamilies, each having diverse biological roles in insects: (1) pyrokinin (PK) includes the pheromone biosynthesis activating neuropeptide (PBAN) and the diapause hormone (DH), (2) the capability (CAPA) peptides, and (3) the ecdysis-triggering hormone (ETH). PBAN as a member of PK subfamily was first identified to stimulate pheromone biosynthesis in moths three decades ago. Since then, PBAN peptides have been extensively studied by various research groups from a broad spectrum of arthropods. In this paper, we briefly review insect PBAN molecules with emphasis on gene structure and expression, signal transduction, physiological mechanism in sex pheromone biosynthesis, and application for pest management.

• Korean Journal of Environment and Ecology
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• v.10 no.1
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• pp.1-13
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• 1996

This study was conducted to investigate the avifauna and to suggest the management discipline for the protection of bird community in Odaesan National Park. Field survey was carried over 2 main trails by line transect method from mid June to early December in 1996. The 1st section included the area from Woljongas to Sangwonsa 7km distance. The 2nd section survey started from Sangwonsa via Bukdaesa and the summit of Odaesan to Sangwonsa 9.8 km distance. The study results were as follows ; The observed birds belonged to 9 orders 22families 52species, they also had Black Woodpecker(Dryocopus martius)designated as natural monument No. 242, Chinese Sparrow Hawk(Accipiter soloensis) and Kestrel(Falco tinnunculus) as natural munumet No. 323, Scops Owl(Out scops)and Korean Wood Owl(Strix aluco) as No. 324. These birds also were classified into 25 species for residents, 16 species for summer visitors, 8 species for passage migrants, 3 species for winter visitors, respectionely. The 2nd section showed a high species richness and individuals in every season, however, had a difference in species composition with 1st section. Nesting guild of breeding bird community used highly in order of bush, hole, and canopy as a nest resources. It is suggested that high bush-nesting guild had a deep relationship with bush layer located in the ecotone of 1st section and that located in the high elevated zone in 2nd section. Hole-nesting guild such as Black Woodpecker(Dryocopus martius), Gray-headed Woodpecker(Picus canus) and Great Spotted Woodpecker(Dendrocopos major) were surveyed only in 2nd section, so it could be attribute to the small fragmentation and the growing of high diameter at breast height(D.B.H) tree in 2nd section. It is urgent that the management of camping ground and people for the conservation of brook in 1nd section, trail protection for the prevention from trail enlargement in 2nd section for the bird protection. Artificial food in snowy winter will provide the good breeding condition with the residents and migrants. And, the endeavor to lessen the habitat fragmentation will be beneficial to the birds who have a large home range such as Black Woodpecker(Dryocopus martius) and Korean Wood Owl(Strix aluco). For the control of Domestic Dove(Columba livia) populations, it could be recommended that the elimination of their nesting resources by net.

• Journal of Animal Science and Technology
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• v.49 no.5
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• pp.677-688
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• 2007

돼지를 포함한 대부분의 동물은 일정한 발정주기를 가지고 일정한 시기에 배란을 하는 자연배란동물이지만, 토끼, 고양이, 밍크 등의 암놈은 교미자극에 의해 배란이 일어나는 유기배란동물이다. 또한 1년에 한 번만 발정하는 단발정동물과 1년에 수차례 발정하는 다발정동물이 있다. 이 중에서 모돈은 1년에 수차례 발정하는 다발정 동물로서 발정기에 들면 비발정기와는 다른 행동을 나타낸다(Diehl 등, 2001). 양돈가의 수익을 최대화하기 위해서는 비생산일수를 최소로 줄여야 한다. 모돈의 비생산일수를 줄일 수 있는 한 가지 방법은 성공적으로 교배를 시키는 것이다. 이처럼 성공적으로 교배를 시키기 위해서는 수정적기를 정확히 예측해야 한다. 만약 수정적기를 정확히 판단하지 못하여 수태가 되지 않으면, 비생산일수가 늘어나 손실을 입게 된다. 따라서 수정적기를 정확히 판단하는 것은 모돈의 성공적인 인공수정에 있어서 중요한 요소이다. 수정적기는 배란이 일어나기 전 10시간에서 12시간 사이이며, 발정이 시작되는 시점을 기준으로 하였을 때 경산돈의 경우 26시간에서 34시간 사이이고 미경산돈의 경우는 18시간에서 26시간 사이이다(Evans 등, 2001). 현재 하루에 두 번 모돈의 발정을 확인하는 것이 일반화되어 있으며, 이 때 웅돈을 접촉시키거나 육안관찰을 통하여 발정 유무를 판단한다. 이러한 방법에는 숙련된 기술과 풍부한 경험이 요구될 뿐만 아니라 총 소요노동력의 30% 정도가 요구된다(Perez 등, 1986). 하루에 두 번밖에 발정을 감지하지 않기 때문에 발정이 언제 시작되었는지를 정확히 알 수 없으며, 또한 발정의 대부분이 새벽에 시작되므로 수정적기를 정확히 판단하기란 매우 어렵다. 만약 발정을 감지했더라도 적기에 인공수정을 하지 못한다면, 수태율이 낮아지므로 경제적 손실이 초래된다. 현재 이러한 문제점 때문에 2회에서 3회에 걸쳐 인공수정을 하고 있으나 이에 따른 소요비용과 소요노동력 등은 양돈가의 부담을 가중시키는 요인이 되고 있다. 돼지는 발정기가 되면 비발정기에 나타내지 않던 외음부의 냄새를 맡는 행동, 귀를 세우는 행동 및 승가허용 행동 등을 나타낸다(Diehl 등, 2001). 또한 돼지는 비발정기에 비하여 발정기에 더 많은 활동량을 나타낸다(Altman, 1941; Erez and Hartsock, 1990). Freson 등(1998)은 스톨에서 개별적으로 사육되고 있는 모돈의 활동량을 적외선센서를 이용하여 측정함으로써 발정을 86%까지 감지하였다고 보고하였다. 그러나 이 연구는 단지 모돈의 발정을 감지하였을 뿐 번식관리에 있어서 가장 중요한 수정적기의 판단 기준을 제시하지 못하였다. 따라서, 본 연구는 스톨에서 사육되는 모돈의 활동량을 측정함으로써 발정시작시각을 감지하고 이를 기준으로 인공수정적기를 예측할 수 있는 인공수정적기 예측 장치를 개발한 후 이의 성능을 농장실증실험을 통하여 시험하고자 수행되었다.

• Journal of Embryo Transfer
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• v.22 no.1
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• pp.15-19
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• 2007

The objective of this study was to investigate the relationship between body condition score (BCS), blood urea nitrogen (BUN) and estrous expression for the purpose of improving reproductive performance. In total, 37 ovulations and 28 estrous detection were observed among 51 Holstein-Friesian dairy cows. The estrous inducement rate and estrous expression rate were significantly lower for cows with BCS below 2.0 than for cows with BCS above 2.0. There was 0% of rate of standing heat in cows with BCS below 2.0 whereas the rate of standing heat was markedly increased in cows with BCS above 2.0 (46.7% and 64.7% for BCS $2.0{\sim}2.49$ and BCS $2.5{\sim}3.0$ cows, respectively). The estrous expression rate was significantly lower for cows with BUN below 10mg/dl than for cows with BUN above 10mg/dl. There was no significant difference among duration time of estrus, estrous behavior patterns and BUN concentration. The rate of estrous expression and concentration of BUN was not significantly different between primiparous and multiparous cows. This result shows that the level of BCS and BUN affect the estrous expression. Considering the situation that estrous expression is decreased in recent years, effective nutritional management should be accompanied to improve reproductive performance.

• Journal of Aquaculture
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• v.4 no.1
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• pp.31-66
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• 1991

This paper deals with the reproductive ecology e.g., number of the pre-spawning moults, morphological characteristics of the pre-spawning moult the common moult, daily ration druing a molting cycle mating behavior, structures of spermatozoa and spermatophore, structure of vas deferens, mechanisms of the oviposition and brooding into the egg-chambers, a suitable time for the artificial mating and fertilization, time sequence of the oviposition and brooding into egg-chambers from the copulation, responses to temperature and chlorinity on the egg development and hatching, effect of temperatures on duration of egg development, physical mechanism of the egg hatching, to make an attempt for the artificial spawning and brooding to establish a suitable system of the artificial seedling-production for the aquaculture. 1. Females molted commonly $8{\~}10$ times at an interval of $17{\~}18$ days at $28^{\circ}C,\;3.26\~4.35\%_{\circ}$ while the prespawning moltings were $4{\~}5$ times at an interval of $13{\~}14$ days. The suitable state for artificial copulation was within 14 hours elapsed from the prespawning moltings (most suitable state was within 8 hours). Males discharged a gelatinous spermatophore and placed it on the females sternum during copulation. Oviposition was seen $6{\~}17$ hours after copulation. External fertilization was considered to take place at oviposition. Fertilized eggs held in egg-chambers forming between pleopods were about $5000{\~}6000$ in females those sizes about 6.5 cm in body length. 2. Eggs immediately after oviposition were elliptic shape, measuring $0.58{\times}0.48$ mm up to hatching. Their sizes increased with egg development and finally reached $0.85{\times}0.54$ mm up to hatching. The relationship between the long axis of the egg(Y in U) and days elapsed(X) was expressed as Y= 5.60194 + 0.007358X. The eggs performed superficial cleavage and their cleavage furrows became visible at the 4-daughter-nucleus stage. The eggs showed normal development up to hatching at water temperature range of $22{\~}30^{\circ}C$ (optimum temperature : $26{\~}28^{\circ}C$) and at chlorinity range of $0.00\~6.64\%_{\circ}$ (optimun chlorinity : $2.21{\%}_{\circ}$). The relationship between incubation period (Y in days) and water temperature(X in $^{\circ}C$) could be expressed as Y= 50.803-1.3555X. The eggs hatched $12{\~}13$ days after oviposition at $28.0{\~}28.6^{\circ}C$ 3. The pre-spawning moltings were appreciably different in the morphologic structure from those of common moltings. Breeding setae and dresses were formed on the thoracic regions, abdominal epimerae and the bases of the first to fourth pleopods in order to prepare and support oviposition, transfering and supporting eggs in egg-chambers up to hatching. These supplementary breeding organs were observed only at reproductive seasons.