• Journal of Embryo Transfer
• /
• v.21 no.4
• /
• pp.323-330
• /
• 2006

본 연구의 목적은 3주 동안 장기간 냉각상태로 보관된 개 정자의 기능적 특성을 알아보고자 두 종류의 정자 희석액, Glucose-BSA(G-BSA), Dimitropoulos-II(BIMI)가 정자의 기능적 특성에 미치는 영향을 알아보고자 한다. 개 정액을 수지법에 의해 채취하여 원심분리한 후 정장은 제거하였다. 정자를 G-BSA나 DIMI으로 희석하여 최종 농도를 $100\times10^6$ sperm/ml로 하였다. 희석된 정자를 정자 운송 시스템을 이용하여 루지애나 주립 대학에서 뉴올리언즈 실험실까지 운송하였다. 정자 운송 시스템은 $20^{\circ}C$에서 $9^{\circ}C$까지 분당 $0.08^{\circ}C$ 냉각율로 설정하였다. 희석된 정자는 $4^{\circ}C$에서 3주간 보관하였다. 보관 1일부터, 매주 단위로 정자의 활력, 정자 원형질막 고유성(생존성), 정자 첨체의 고유성을 검사하였다. 또한 체외조건하에서의 정자 번식 능력을 평가하기 위해 zona binding assay를 실시하였다. 실험 결과 냉각 상태로 3주 동안 보관된 개 정자는 여전히 정자의 기능적 특성을 나타냈다. 냉각기간이 길어질수록 정자의 활력 및 생존성은 감소하였으며(P<0.01), 첨체의 고유성은 냉각기간에 따라 감소하였으나 유의성은 없었다. 정자의 특성 중 활력이 다른 특성에 비해 냉각처리에 민감한 반응을 보였다. G-BSA배지에 보관된 정자의 활력 및 생존성이 DIMI에 비해 높게 나타났으며, 1 주 동안 보관된 정자의 생존성의 비교시 유의성이 높게 나타났다(P<0.05). 그러나 첨체의 고유성은 1일 동안 DIMI에 보관된 정자에서 높게 나타났다(P<0.05). 3주 동안 냉각상태로 보관된 개 정자는 여전히 난자의 투명대에 결합하는 능력을 보였으며, G-BSA에 보관된 정자의 경우 난자에 부착되는 평균 정자 수가 보관일수에 따라 유의적으로 감소하였다(P<0.05). 그러나 희석액 종류에 따른 난자에 부착되는 평균 정자 수의 유의적 차이는 없었다. 정자의 활력 및 생존성과 난자의 투명대에 결합하는 정자 수와의 상관관계를 본 결과, 활력 및 생존성이 높은 정자일수록 난자의 투명대에 많이 결합하여 정자의 잠재적 번식력이 증가함을 보였다. 결론적으로 G-BSA나 DIMI의 희석액으로 희석된 후 $4^{\circ}C$에서 냉각 보관된 정자는 3주 동안 정자의 기능적 특성을 유지하였으며 냉각 보관 2주까지 좋은 성적의 정자 특성을 나타냈다. 개 정자 냉각 보존에 대안적인 희석액으로 G-BSA도 사용 가능한 것으로 사료된다.

• Journal of Animal Science and Technology
• /
• v.46 no.6
• /
• pp.957-966
• /
• 2004

The objective of this study was to investigate the effects of BPA on reproductive characteristic and blood hematological and chemical values in mice. The male mice were intraperitoneally injected BPA in native control(no treatment), positive control(corn oil, 3$m\ell$/kg B.W), 0.05, 0.5 and 5.0mg BPA/kg B.W and female mice were injected BPA in control(corn oil, 3$m\ell$/kg B.W), 0.05, 0.5 and 5.0mg BPA/kg B.W with 5 times at 3 days interval for 14 days. The administration of BPA in male mice didn't affect the body and reproductive organ weight such as testis, epididymis, vesicular gland and coagulating gland. We found that the 5.0mg BPA group was significantly reduced the sperm concentration and increased the sperm abnormality compared to native, positive control and 0.05mg BPA groups(P<0.05), but any other effects were not found in sperm viability and motility in BPA treatment groups. The RBC, HB, HT, MCV, MCH, MCHC, albumin, BUN and total protein of blood hematological and chemical values in male were not different in all experimental groups(P>0.05). However, the value of WBC was slightly lower in BPA treatment groups than that of control groups and PLT was slightly higher in BPA groups than that of control groups, but not significantly defference among the experimental groups(P>0.05). In female mice, the effects of BPA on body weight didn’t affect in all experimental groups, but ovary weights in 0.5 and 5.0mg BPA groups were significantly increased compared to those in control and 0.05mg BPA group(P<0.05). The uterine weight in BPA groups was slightly higher than that of control group, but not significantly different in all experimental groups(P>0.05). The RBC, Hb, HT, MCV, MCH, MCHC, albumin and total protein of blood hematological and chemical values in female were not different in all experimental groups(P>0.05). The values of WBC and PLT in BPA groups were slightly higher than that of control, but not significantly different among the experimental groups. The concentration of BUN was the higher in BPA groups than that of control group. The histological evaluation of testis, ovary and uterus were not different in all experimental groups.

• Journal of Korea Water Resources Association
• /
• v.41 no.4
• /
• pp.423-432
• /
• 2008

The local migration or movement behavior of fishes in streams are related to feeding, spawning, growing, dispersing, and refuging. The pale chub (Zacco platypus) is a dominant species that migrates locally and inhabits in river and stream in Korea. However, dams, weirs, culverts and other regulatory structures are physical barriers that limit fish movement and fragment habits and populations. If main stream and off-channel habitats are connected with culverts, they would restrict the small fish as pale chub movement due to the high flow velocities and low depths. But in Korea, there is no experimental study to evaluate the swimming performance of species in Korea. Therefore, it is difficult to proposed that design guidelines for pass fishes through culverts. The purpose of this experimental study is to evaluate the swimming performance of pale chubs. A series of swimming performance test has been used in both of the fixed velocity and the incremental velocity methods in an experimental flume. As a result, the critical swimming speed for pale chub(body length 8.9 cm) was found to be about 0.7 m/s. Therefore, the flow velocity for culvert design in the low flow condition should not be exceed the its swimming ability, especially 0.7 m/s for pale chubs(body length 8.9 cm). And the minimum depth for culvert design in the low flow condition should not be lower than the fish body height add a dorsal fin height.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
• /
• 2018.11a
• /
• pp.63-65
• /
• 2018

조류는 조석현상에 따라 해수가 이동하기 때문에 일어나는 흐름으로 유향, 유속이 주기적으로 변하며, 일반적으로 반일의 주기를 가지고 있으나 장소에 따라 약 하루의 주기로 유동하는 곳도 있다. 조류에 의한 해수의 이동은 대량의 열량과 수량을 운반 할 뿐만 아니라 그 속에 함유된 각종 성분 및 기타 여러 가지 부유물을 운반함으로써 각종 성분의 분포, 어류의 회유, 번식 등에 관계가 있으며 어류생활에 직접, 간접적으로 많은 영향을 미치고 있다. 본 연구에서는 해수유동의 흐름이 활발한 서해 중부 연안의 관측자료를 이용하여 계절별 조류특성을 파악하였다.

• Horticultural Science & Technology
• /
• v.33 no.3
• /
• pp.448-452
• /
• 2015

'Seeberry' is a short-day $F_1$ hybrid strawberry for seed propagation, that was released by the Protected Horticulture Experiment Station (RDA, Republic of Korea) in 2013. Inbred lines derived from domestic germplasms and selected varieties were used to develop this cultivar. 'Seeberry' originated from a cross of two inbred lines: a male parent 'Wongyo 3115' with high firmness and a female parent 'Wongyo 3116' with excellent fruit shape and high yield. 'Seeberry' cultivar should be grown with a crown diameter of over 10 mm to reveal its unique characteristics. 'Seeberry' has an upright plant shape with elliptic leaves, 12-15 flowers per cluster, vigorless growth habit, and average flower bud differentiation compared to vegetatively propagated cultivars. Fruits of 'Seeberry' are conical, having light skin color and approximately 15-16 g in average weight. Yield of this cultivar is about 92% lower than that of 'Akihime', but 'Seeberry' has excellent taste, sugar content of $9.7^{\circ}Bx$, acidity of 0.6%, and good texture. With regard to disease and pest resistance, 'Seeberry' is sensitive to powdery mildew, aphids, and the two-spotted spider mite, and is resistant to anthracnose.

• Korean Journal of Environment and Ecology
• /
• v.22 no.5
• /
• pp.471-480
• /
• 2008

This study was conducted to investigate the characteristics of breeding bird community in relation to altitude and vegetation in Jirisan National Park. The survey was carried over 4 study sites by point counts method to figure out habitat environment and breeding bird community from March to August in 2006. The study results are summarized as follows: Total 32 species were recorded, and 27 species and density of 37.31 ea/ha in low altitude mixed forest, 23 species and 34.99 ea/ha in low altitude deciduous forest, 18 species and 23.95 ea/ha in high altitude mixed forest, 19 species and 20.21 ea/ha in high altitude deciduous forest, respectively. Eleven species were observed only in the low altitude sites, 4 species were observed only in the high altitude sites. Number of species and density were high in the low altitude sites, and they were high in the mixed forests. In nesting guild analysis, the low altitude sites are similarly found species number of three types but canopy nesting species in the high altitude sites are advent less. In foraging guild analysis, the species number of canopy foraging appeared most highly in all study sites. In the difference analysis of each species density. Four species which are showed the difference in the low altitude sites, owing to vegetation. Long-tailed Tit(Aegithalos caudatus) and Great tit(Parus major) are difference because of difference in volume of canopy layer, and Coal Tit(Parus ater) was difference because of coniferous forest preference quality. Four species(Hazel Grouse, Winter Wren, Pale Thrush, Yellow-throated Bunting) which are showed the difference of the density in the high altitude sites because of thick growth of the bush layer. Ten species which are showed the difference in study sites, owing to altitude. Oriental Cuckoo(Cuculus saturatus), Winter Wren(Troglodytes troglodytes), Siberian Blue Robin(Luscinia cyane), Arctic Warbler(Phylloscopus borealis), Coal Tit(Parus ater), and Yellow-throated Bunting(Emberiza elegans) appeared highly in the high altitude sites, Pale Thrush(Turdus pallidus), Long-taild Tit(Aegithalos caudatus), Varied Tit(Parus varius), and Eurasian Nuthatch(Sitta europaea) appeared highly in the low altitude sites. It seems that bush layer coverage volume and canopy layer total coverage volume do influences on the breeding bird community, because the bush layer was thick growth, and canopy layer coverage volume was difference. It would be needed the management and maintenance of bush layer coverage volume and canopy layer with multi-layer structure to increase foliage height diversity and total coverage volume for the protection and management of bird community in Jirisan National Park.

• Korean Journal of Weed Science
• /
• v.3 no.1
• /
• pp.23-28
• /
• 1983

To study ecological characteristics of Eleocharis kuroguwai occuring in Korea its propagules were collected from 6 locations from the northern part to the southern part of Korea (Chuncheon, Suweon, Iri, Jeonju, Gwangiu and Milyang) in 1981, cultured and replanted 3 times (May 20, June 5, June 20) in 1982. They flowered from August 5 to August 27 when they were planted on May 20, and from August 20 to August 27 when they were planted on Tune 20. Plant height, number of tillers and top fresh weight/$m^2$ were 50-90cm, 500-875, and 175-750g, respectively when they were planted on May 20, and 40-70cm, 250-625, 325-625g, respectively when they were planted on June 20. Number of tubers per plant were 0.98-1.98 when they were planted on May 20, and 1.81-2.87 when they were planted on June 20. Eleocharis kuroguwai from Chuncheon or Suweon was more open in plant type, shorter in plant height, narrower in diameter of pedicel and shorter in inflorescence than those from Iri, Jeonju or Gwangju. Each of the local collections may be regarded as different ecotype, based on the above differences in morphology and responses in growth and flowering to the planting dates. The results appear to imply that Eleocharis kuroguwai weeds occurring in various locations of Korea are different one another in competitive avility with rice crop.

• Journal of the Korean Institute of Landscape Architecture
• /
• v.46 no.6
• /
• pp.111-119
• /
• 2018

This study was carried out to investigate the growth characteristics and propagation methods of the Korean native Amelanchier asiatica, A. arborea, and A. alnifolia as fruit trees for gardens. Due to the lack of recent research on Amelanchier spp., their superficial classification is still unclear and the names are being used interchangeably. The results are obtained as follows : A. arborea and A. alnifolia were globular type multi-stemmed shrubs. A 20-year-old tree of A. asiatica was 7.8m in height, with a 5.2m crown width, with one trunk. As for the morphological characteristics, leaves of A. asiatica were oblong, with an acuminate of, 6.1cm and 3.6cm width, but A. arborea and A. alnifolia had acute obovate leaves. The leaf size of A. alnifolia was the largest among the three species. The flower size of A. asiatica was bigger than that of A. arborea and A. alnifolia. In addition, its petals and flower clusters were also the largest among the three species. The flowering of A. asiatica initiated on April 21 and then bloomed for a duration of 24 days in Osan, while that of A. arborea and A. alnifolia initiated flowering on April 12 and then bloomed for a duration of 22 days in the same location. The fruit of A. arborea and A. alnifolia were green on May 10~12, it changed into purplish red on May 24~26, and its matured on June 1~3. The duration of fruit persistence of A. arborea and A. alnifolia were 48~50 days. On the other hand, A. asiatica showed greenish fruit on May 20, it became red on September 4, and had fallen by October 3. The fruit size was the largest at 1.03cm of height and 1.12cm of diameter in the A. arborea, followed by the big berry of A. alnifolia and the smallest fruit in the native, A. asiatica. It was difficult to root due to the hardwood cutting of A. arborea at a 40% rate of rooting. In the softwood cutting, the rooting rate of A. arborea was increased by the treatment with concentrated IBA, especially at 5,000 and 7,000ppm. The optimum date for cutting was on June 27, when the rooting rate was more than 80%. The most effective method for rooting of A. arborea was rootone or 7,000 ppm IBA treatment on June 27 softwood cuttings, which showed a rooting rate of over 80%.

• Korean Journal of Ecology and Environment
• /
• v.50 no.1
• /
• pp.116-125
• /
• 2017

Alpine plants with a scarcity of pollinators in harsh environments have been believed to undergo selfing for reproductive assurance; however, contradictory evidence is also available. Snowmelt regimes in alpine areas function to change life history characteristics of alpine plants such as flowering time and duration; yet the effects of snowmelt regimes have never been tested in alpine plants in Korea. This study was conducted to investigate the dichogamy, mating systems, and flowering characteristics of Megaleranthis saniculifolia populations [early and late snowmelt plots (ESP and LSP, respectively)] in a subalpine area of Sobaeksan Mountain in Korea. M. saniculifolia exhibited incomplete protogyny in that despite early maturation of pistils, maturation times of pistils and stamens within flowers were partly overlapped. Control and hand-outcrossing treatments produced significantly higher number of follicles and seeds per flower than autonomous and hand-selfing treatments. Based on the aggregate fruit set, the auto-fertility index (AI) and self-compatibility index (SI) were 0.33 and 0.50, respectively. Snowmelt occurred 10 days earlier in ESP than in LSP, thereby ESP and LSP showed distinct differences with regard to flower longevity and season, but showing no difference in peak flowering dates. We concluded that M. saniculifolia is an incomplete protogynous and largely outcrossing plant requiring pollinator service. Temporal variation in snowmelt time and subsequent changes in flowering characteristics under climate change may further threaten the population persistence of M. saniculifolia which has already been designated as endangered species in Korea.

• Journal of the Korean Society of Marine Environment & Safety
• /
• v.12 no.2 s.25
• /
• pp.75-79
• /
• 2006

In order to study the characteristics of water quality in coastal water and mechanism of phytoplankton blooms, factors of water quality were investigated in diatom bloom area due to rainfall event and no diatom bloom area Diatom blooms occurred after heavy rain and the dominant species were Skeletonema costatum($1,200{\sim}5,000cells/mL$) and Thalassiosira spp.($750{\sim}1,200cells/mL$). In diatom bloom area, water temperature, pH, and dissolved oxygen were observed at higher level than in no diatom bloom area Although these two areas were only 20 meters apart from each other, sharp difference in coastal water quality between two areas was observed. In diatom bloom area, concentrations of nitrogen, phosphorus, and silicate were observed at lower level than in no diatom blooms area. This seems to be due to inflow of much trace metal such as Fe, Mo, Se and so on than nitrogen, phosphorus, and silicate by rainfall events. However, distinct differences in DIN/DIP and $DIN/SiO_2-Si$ between these two areas were not observed.