• Title/Summary/Keyword: 방해 최소화

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Comparision of Preparation Methods for Water Soluble Vitamin Analysis in Foods by Reversed-Phase High Performance Liquid Chromatography (역상 고속 액체 크로마토그래피에 의한 식품 중 수용성 비타민 분석을 위한 전처리법의 비교)

  • Kim, Hyung-Soo;Jang, Duck-Kyu;Woo, Dong-Kyun;Woo, Kang-Lyung
    • Korean Journal of Food Science and Technology
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    • v.34 no.2
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    • pp.141-150
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    • 2002
  • Owing to a need for simple extraction and purification for analysis of water soluble vitamins in food samples by RP-HPLC with UV-detector, the methods of bromelain and protease hydrolysis and $C_{18}$ Sep-Pak solid phase extraction were employed. The recoveries of standard water soluble vitamins by the bromelain and protease hydrolysis and $C_{18}$ Sep-Pak solid phase extraction were significantly high compared to AOAC methods in most of vitamins. The contents of pyridoxal determined with protest in the pork was similar, but in the bromelain hydrolysis and AOAC method, was high compared to the results of reference. The niacinamide, thiamin and riboflavin determined with bromelain and protease hydrolysis showed similar values to the results of references. In the potato, pyridoxamine was detected in the AOAC method, which was not detected in the bromelain and protease hydrolysis methods. Pyridoxal contents in the protease hydrolysis and AOAC methods were very similar to the results of references. The recoveries of fortified standard vitamins in food samples were significantly high and accurate compared to those of AOAC methods. The extraction and purification with $C_{18}$ Sep-Pak solid extractor might be considered superior method for the determination of water soluble vitamins in food samples.

An approach to capture travelers' choice behaviour in response to unexperienced transportation modes: A case study of Personal Rapid Transit (미경험 교통수단에 대한 이용자 선택행태 분석: Personal Rapid Transit 사례를 중심으로)

  • Yu, Jeong-Whon;Shin, Seung-Kwon;Choi, Jung-Yoon
    • Proceedings of the KSR Conference
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    • 2011.10a
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    • pp.1730-1738
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    • 2011
  • Personal Rapid Transit (PRT) has emerged as a promising alternative transportation mode for transit-oriented sustainable communities by creating compact and walkable environments with competitive construction and operational costs. This study seeks to capture the changes in travel mode choice behavior in response to the introduction of PRT to travelers who have no previous experience of using it. A critical issue in modeling the PRT mode choice is how to capture travelers' perception and evaluation of the unexperienced travel mode. The data used come from questionnaire surveys, in which RP (Revealed Preference) and SP (Stated Preference) data were collected in relation to travel mode choices with and without PRT systems. The questionnaire was designed especially for mitigating the potential bias in favor of or against choosing PRT. In addition, an efficient approach was proposed to reduce the number of SP questions by avoiding the complex fractional factorial design which tends to make it difficult for respondents to keep their attention throughout the survey. The analysis results show that the proposed approach is able to realistically capture the effects of explanatory variables on the travel mode choice. Discrete choice models are developed to predict travelers' mode choices under different choice scenarios by varying PRT system specifications and operational characteristics. PRT patronages are projected for two different test sites using the developed PRT mode choice models.

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Development of Portable-Type Electrode for the Determination of Highly Concentrated Hydrogen Peroxide (휴대용 고농도 과산화수소 측정 전극의 개발)

  • Lee, Jin Seo;Cui, Gang;Kim, Sang Jin;Cha, Geun Sig;Nam, Hakhyun;Rho, Kyung Lae;Kim, Jin Doo
    • Analytical Science and Technology
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    • v.12 no.2
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    • pp.125-129
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    • 1999
  • Portable-type two electrode system for the determination of highly concentrated hydrogen peroxide (10-75%) has been studied. The electrode body was made with teflon rod (length=10 cm, diameter=1.5 cm) to withstand the highly corrosive power of hydrogen peroxide. Glass carbon rod (diameter=3 mm) was used as the working electrode and a carbon cylinder (i.d.=5 mm; o.d.=9 mm) was used as counter electrode. The applied voltage for the determination of $H_2O_2$ was 0.8 V. Diluting the highly concentrated samples taken from the industrial batch to 10% or less, it was possible to make quantitative determinations, while eliminating the interference from the stabilizer contained in the sample and preventing the surface of the electrode from oxidative corrosion. Employing hydrogen peroxide permeable membrane (teflon membrane${\leq}100{\mu}m$) for the electrode system, it was possible to measure the content of $H_2O_2$ in highly concentrated samples directly, quantitatively and reproducibly with no extra dilution step. However, it was necessary to change the internal electrolyte frequently to maintain the analytical performance of the electrode.

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The Evaluation of Patients' Radiation Dose During TACE of Interventional Radiology (TACE의 중재적 시술시 환자의 피폭선량 평가)

  • Lee, Seung-Youl;Lim, Hyun-Soo;Han, Man-Seok
    • Journal of radiological science and technology
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    • v.34 no.3
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    • pp.209-214
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    • 2011
  • Goal of this study was to measure effective radiation dose of highly exposed patients who were treated by TACE, interventional radiology from June to September 2010. The effective radiation dose was approximately measured by weighted DAP (dose area product) with the ionization chamber which is inserted in angiography equiment (Philips Allura Xper FD 20). Radiation dose was measured by TLD which was attached to patients' thyroid and genital gland. The average of ED (effective dose) was 18.43${\pm}$6.63 mSv per person and the average of radiation dose of thyroid and genital gland was 0.37 mSv, 0.77 mSv, respectively. The mean radiation dose of operators who wear the protector was 0.07 mSv for thyroid, and 0.01 mSv for genital gland, respectively. All staffs involved in TACE treatment, have to keep them aware and use the appropriate protectors to reduce the radiation dose of patient.

Characteristics of Wastewater Treatment in Applying RBC Modified Dephanox Process (회전원판법을 적용한 Modified Dephanox 공정의 하수처리특성)

  • Kang, Min-Koo;Kim, Keum-Yong;Ryu, Hong-Duck;Lee, Sang-Ill
    • Journal of Korean Society of Environmental Engineers
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    • v.32 no.5
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    • pp.477-486
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    • 2010
  • This study was performed with the object in which it improves the nitrification by using RBC, that is one of the biological waste water treatment process. By applying the Modified Dephanox process to RBC in this research in order to evaluate the improvement of the nitrification by RBC a research was conducted. There is the most conspicuous feature of the process of using RBC. it is that the nitrification can be smoothly performed even if the suspended solid of the high concentration as the interference factor in the nitrification tank is flowed in. Moreover, as a result of experiment, TCOD removal efficiency of the process showed up more than about 90%. when influent TCOD loading rate was 0.04~0.1 kg / $day{\cdot}m^3$. and T-N removal efficiency is high at about 75% in spite of the process operating of the laboratory scale was observed. Also, As increasing influent ${PO_4}^{3-}$-P, T-P loading rate, ${PO_4}^{3-}$-P, T-P removal efficiency was increased. Finally, it was elucidated that the utilization of RBC in external nitrification system resulted in not only high nitrification performance but also stable system operation by minimizing inhibitory effect of overflowed suspended solid (SS).

Separatipon of Oryzanol from the Refining By-Product of Rice Bran Oil (미강유 정제 부산물로부터 오리자놀 분리)

  • Kim, In-Hwan;Kim, Chul-Jin
    • Korean Journal of Food Science and Technology
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    • v.23 no.1
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    • pp.76-80
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    • 1991
  • To isolate oryzanol from the by-product of rice bran oil refinning, experiment of solvent fractional crystallization was carried out at various conditions with the dark oil obtained by acidifying the soap stock of micella refinning process and the pitch obtained from vacuum distillation of the dark oil. The impurity interfering the crystallization process such as waxes can be removed as precipitates by cooling the 1:1 mixtrue of acetone and dark oil to $0^{\circ}C$, From the dewaxed dark oil, oryzanol concentrate with 51.3% purity was obtained by fractional crystallization at$0^{\circ}C$ with the mixture of 8 part volume of hexane and 1 part of the dewaxed dark oil. The concentrate was recrystallized at room temperature with 20 part volume of methanol to yield oryzanol crystal of 98.3% purity. The optimum condition of vacuum distillation was temperature of $180^{\circ}C\;at\;0.2{\sim}0.4\;torr$ with 2% steam sparging. At this condition, the free fatty acid in the dark oil was removed as distillate without thermal deomposition to yield 82.3% of oryzanol as the pitch of 27.3% purity. After concentration from the pitch with 20 part volume of hexane to yield yellow powder of 75.4% purity, the yellow powder was recrystallized in methanol at room temperature to obtain the crystal containing 99.0% oryzanol. The overall oryzanol yield from the dark oil and the pitch was 9.5 and 28.5%, respectively. The change of the composition of sterols and triterpenoid alcohols in the compounds isolated during fractionation was analyzed by GC-MS.

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Efficacy of Arthroscopic Diagnosis and Treatment for Acute Complete Metacarpophalangeal Ulnar Collateral Ligament Tears of the Thumb (무지 중수 수지 관절 척측 측부 인대 급성 완전 파열의 진단 및 치료에 대한 관절경의 유용성)

  • Chun, Churl-Hong;Kim, Dong-Chul;Chin, Byoung-Soo;Kim, Chae-Geun
    • Journal of Korean Orthopaedic Sports Medicine
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    • v.4 no.1
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    • pp.49-54
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    • 2005
  • Purpose: Complete rupture of metacarpophalangeal ulnar collateral ligament of thumb needs surgical exploration and repair, owing to the interposition of the adductor aponeurosis (Stener lesion) which interferes in healing process. We performed arthroscopic diagnosis and treatment on ulnar collateral ligament injury of thumb and evaluated it's efficiency. Materials and Methods: Arthroscopy was perfomed on 13 patients of whom injured on complete ruture of metacarpophalangeal ulnar collateral ligament. Follow-up period was over 1 year and mean age was 35.6 years old. Ulnar collateral ligament tears and Stener lesion were diagnosed and treated by arthroscopy procedure. Results were interpreted by joint instability, pinch power, grip power and range of motion on metacarpophalangeal joint of thumb. Results: We found 5 Stener lesions in 13 cases. There was no appreciable postoperative instability. Pinch and grip power were recovered to 92%, 94% of uninjured thumb respectively. Range of motion on metacarpophalangeal joint was mean $52^{\circ}$, almost equal to uninjured thumb. Conclusions: Arthroscopic treatment in metacapophalangeal ulnar collateral ligament injury of thumb is useful method that Stener lesion can be clearly comfirmed and treated. Also soft tissue injuries can be minimized, thereby early functional recovery can be expected.

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Binding of the His-tagged Tail Protein J of Bacteriophage Lambda with Escherichia coli K-12 (히스티딘으로 표지된 람다 박테리오파아지 꼬리 단백질 J와 대장균 K-12와의 결합)

  • Shin, Hae Ja
    • Journal of Life Science
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    • v.28 no.1
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    • pp.78-82
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    • 2018
  • Detection of pathogenic microorganisms takes several days by conventional methods. It is necessary to assess microorganisms in a timely manner to reduce the risk of spreading infection. For this purpose, bacteriophages are chosen for use as a biosensing tool due to their host specificity, wide abundance, and safety. However, their lytic cycle limits their efficacy as biosensors. Phage proteins involved in binding to bacteria could be a robust alternative in resolving this drawback. Here, a fragment of tail protein J (residues 784 to 1,132) of phage lambda fused with 6X His-tag (6HN-J) at its N-terminus was cloned, overexpressed, purified, and characterized for its binding with microorganisms. The purified protein demonstrated a size of about 38 kDa in sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE) and bound with anti-His monoclonal antibodies. It bound specifically to Escherichia coli K-12, and not Salmonella typhimurium, Bacillus subtilis, or Pseudomonas aeruginosa in dot blotting. Binding of the protein to E. coli K-12 inhibited about 50% of the in vivo adsorption of the phage lambda to host cells at a concentration of $1{\mu}g/ml$ 6HN-J protein and almost 100% at $25{\mu}g/ml$ 6HN-J. The results suggest that a fusion viral protein could be utilized as a biosensing element (e.g., protein chips) for detecting microorganisms in real time.

A Study on the Application Methodology of Set-based Design Approach of Outrigger System based on Lean Process (린 프로세스 기반 아웃리거 시스템의 Set-based Design 적용 방안에 관한 연구)

  • Lee, Seung-Il;Cho, Young-Sang
    • Korean Journal of Construction Engineering and Management
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    • v.12 no.4
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    • pp.50-58
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    • 2011
  • Lean concept is management philosophy that defines a customer's value and eliminates wasteful and impeditive factors. Management philosophy of Lean in the construction industry is referred to as "Lean Construction". Now this concept has expanded to achieve effective productivity during the design phase. Currently the norm of the domestic design process has been Point-based Design(PBD). It involves selecting a single structurally-feasible design option early and then refining that single design as more information becomes available throughout the design process. This single design is then re-worked until a solution is found that is feasible for all parties. On the contrary, Set-based Design(SBD) is based on lean processes to eliminate waste and improve project productivity. It focuses on keeping the design space as open as long as possible, to allow "subdesign" to advance and not labeling them as secondary in importance. Preserving the maximum number of feasible designs as long as possible reduces the likelihood that rework will be necessary and allows all project participants to utilize their unique expertise to make the project successful. This study proposes that the design methodology of minimizing waste and increasing productivity through SBD of AHP, one of the decision making process so as to compare PBD with SBD and tries to find decision making process and then suggest that application methodology through performs case study of SBD process.

A Study on the Noise Reduction Method for Data Transmission of VLBI Data Processing System (VLBI 자료처리 시스템의 데이터 전송에서 잡음방지에 관한 연구)

  • Son, Do-Sun;Oh, Se-Jin;Yeom, Jae-Hwan;Roh, Duk-Gyoo;Jung, Jin-Seung;Oh, Chung-Sik
    • Journal of the Institute of Convergence Signal Processing
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    • v.12 no.4
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    • pp.333-340
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    • 2011
  • KJJVC(Korea-Japan Joint VLBI Correlator) was installed at the KJCC(Korea-Japan Correlation Center) and has been operated by KASI(Korea Astronomy and Space Science Institute) from 2009. KJNC is able to correlate the VLBI observed data through KVN(Korean VLBI Network), VERA(VLBI Exploration of Radio Astrometry), and JVN(Japanese VLBI Network) and its joint network array. And it is used exclusively as computer in order to process the observed data for the scientific purpose KJJVC used the VSI(VLBI Standard Interface) as the VLBI international standard at the data input-output specification between each component. Especially, for correlating the observed data, the data is transmitted with 1024Mbps speed between Mark5B high-speed playback and RVDB(Raw VLBI Data Buffer). The EMI(Electromagnetic lnterference), which is occurred by data transmission with high-speed, cause the data loss and the loss occurrence is frequently often for long transmission cable. Finally it will be caused the data recognition error by decreasing the voltage level of digital data signal. In this paper, in order to minimize the data loss by measuring the EMI noise level in transmission of the VSI specification, the 3 methods such as 1) RC filtering method, 2) lmpedance matching using Microstrip line, and 3) Signal buffering method using Differential line driver, were proposed. To verify the effectiveness of each proposed method, the performance evaluation was conducted by implementing and simulations for each method. Each proposed method was effectively confirmed as the high-speed data transmission of the VSI specification.