• Title/Summary/Keyword: 방사선학적 평가

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Evaluation of DNA Damage Induced by Mercury Chloride (II) and Ionizing Radiation in the Earthworm (염화수은(II)과 이온화 방사선 처리에 따른 토양 내 환형동물의 DNA 손상 측정)

  • Ryu, Tae-Ho;Nili, Mohammad;An, Kwang-Guk;Kim, Jin-Kyu
    • Korean Journal of Environmental Biology
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    • v.28 no.4
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    • pp.212-217
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    • 2010
  • Soil pollution by heavy metals has become a significant environmental concern due to a variety of human activities. Specially toxicity caused by excessive mercury exposure is now being recognized as a widespread environmental problem and is continuing to attract a great deal of public concerns. The earthworms are very important animals that aerate the soil with their burrowing action and enrich the soil by decomposing organic matters. Especially the earthworm Eisenia fetida is routinely used in ecotoxicological studies. The levels of DNA damage in earthworms treated with HgCl2 and ionizing radiation were investigated in this study. Genotoxic effects were evaluated in the earthworm's coelomocytes using the comet assay (Single Cell Gel Electrophoresis; SCGE). The results showed that the mercury chloride and radiation were responsible for the genotoxic effects on earthworms. The level of DNA damage significantly increased after the treatment of mercury chloride combined with ionizing radiation. The combined treatment of $HgCl_2$ and ionizing radiation had a greater genotoxicity. This study is amenable to further study such as enzyme activation assay.

Effects of Radiation Mutant Perilla frutescens var. crispa in Preventing and Alleviating Symptoms in a Monosodium Iodoacetate-Induced Osteoarthritis Rat Model (MIA로 퇴행성관절염을 유도한 랫드에 방사선 형질전환 차조기가 증상 예방 및 완화에 미치는 효과)

  • Sim, Boo-Yong;Joo, In-Hwan;Kim, Sung-Kyu;Ji, Joong-Gu
    • Journal of the Korean Applied Science and Technology
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    • v.37 no.4
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    • pp.830-838
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    • 2020
  • The present study aimed to evaluate the effect of radiation mutant Perilla frutescens var. crispa on bone metabolism and the inflammatory response in a monosodium iodoacetateinduced rat model of osteoarthritis. radiation mutant Perilla frutescens var. crispa was administered orally at doses of 25, 50 or 100 mg/kg/day for 2 weeks before direct injection of monosodium iodoacetate (3 mg/50 μl of 0.9% saline) into the intra-articular space of the rats' right knees. The rats subsequently received the same doses of oral radiation mutant Perilla frutescens var. crispa for another 4 weeks. It was evaluated that the treatment effects based on serum biomarkers, and morphological and histopathological analysis of the knee joints. Compared with those in control rats, the radiation mutant Perilla frutescens var. crispa treatments significantly reduced the serum levels of inflammation, bone metabolism markers (i.e., COX-2, LTB4, MMP-3, and COMP), and the amount of fibrous tissue. Otherwise, it was significantly increased the concentration of TIMP-1 and calcitonin. In addition, the radiation mutant Perilla frutescens var. crispa treatments effectively preserved the knee cartilage and synovial membrane. As a result, it indicates that radiation mutant Perilla frutescens var. crispa prevented and alleviated osteoarthritis symptoms. Thus, radiation mutant Perilla frutescens var. crispa can be used in food and drug material for the management of osteoarthritis.

Radiation Protection Effects of Schizonepeta Tenuifolia Water Extracts by Gender in SD Rats (SD rats의 성별에 따른 형개 열수추출물의 방사선 방호효과)

  • Lee, Ji-Eun;Kim, Jang-Oh;Lee, Yoon-Ji;Jeon, Chan-hee;Heo, Sung-Hoe;Min, Byung-In
    • Journal of the Korean Society of Radiology
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    • v.15 no.3
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    • pp.313-320
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    • 2021
  • This study evaluates the radiation protection effects of Schizonepeta tenuifolia water extract on white rat by gender. Schizonepeta tenuifolia contains polyphenol and flavonoid, which are typical substances that remove free radical. Thus, to determine the effectiveness of radiation protection, the Schizonepeta tenuifolia water extract was administered to Sparaqu-Dawely (SD) rat males and females for two weeks, followed by hematological analysis, analysis of changes in the length of the small intestine villi length, and SOD activity evaluation. The male rat in ST+IR group showed a slightly greater recovery of platelets than the IR group. The ST+IR group also showed a higher platelet recovery capability than the IR group. lymphocyte showed that male and female rat ST+IR groups have higher resilience than IR groups likewise platelet. After irradiation, the villi length of the male rat and female rat decreased less in ST+IR group than in the IR group, indicating that the villi length of all genders was less damaged. This result confirmed that the Schizonepeta tenuifolia water extract had a radiation protection effect.

Dosimetric Comparison of Intensity Modulated Radiation, Proton Beam Therapy and Proton Arc Therapy for Para-aortic Lymph Node Tumor (대동맥림프절 종양에 대한 세기조절방사선치료, 양성자치료, 양성자회전치료의 선량 비교평가)

  • Kim, JungHoon
    • Journal of radiological science and technology
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    • v.37 no.4
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    • pp.331-339
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    • 2014
  • To test feasibility of proton arc therapy (PAT) in the treatment of para-aortic lymph node tumor and compare its dosimetric properties with advanced radiotherapy techniques such as intensity modulated radiation therapy (IMRT) and conventional 3D conformal proton beam therapy (PBT). The treatment plans for para-aortic lymph node tumor were planned for 9 patients treated at our institution using IMRT, PBT, and PAT. Feasibility test and dosimetric evaluation were based on comparisons of dose volume histograms (DVHs) which reveal mean dose, $D_{30%}$, $D_{60%}$, $D_{90%}$, $V_{30%}$, $V_{60%}$, $V_{90%}$, organ equivalent doses (OEDs), normal tissue complication probability (NTCP), homogeneity index (HI) and conformity index (CI). The average doses delivered by PAT to the liver, kidney, small bowel, duodenum, stomach were 7.6%, 3%, 17.3%, 26.7%, and 14.4%, of the prescription dose (PD), respectively, which is higher than the doses delivered by IMRT (0.4%, 7.2%, 14.2%, 15.9%, and 12.8%, respectively) and PBT (4.9%, 0.5%, 14.12%, 16.1% 9.9%, respectively). The average homogeneity index and conformity index of tumor using PAT were 12.1 and 1.21, respectively which were much better than IMRT (21.5 and 1.47, respectively) and comparable to PBT (13.1 and 1.23, respectively). The result shows that both NTCP and OED of PAT are generally lower than IMRT and PBT. This study demonstrates that PAT is better in target conformity and homogeneity than IMRT and PBT but worse than IMRT and PBT for most of dosimetric factor which indicate that PAT is not recommended for the treatment of para-aortic lymph node tumor.

Development of the Database System for Managing Decommissioning Information from Research Reactor (연구로 해체정보 관리를 위한 데이터베이스 시스템 개발)

  • 정관성;이동규;박희성;이근우;오원진
    • Proceedings of the Korean Radioactive Waste Society Conference
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    • 2004.06a
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    • pp.58-71
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    • 2004
  • Korea Research Reactor (KRR) 1&2 has shut down and through preparatory activities, its decommissioning activities are underway. As its decommissioning activities have been done over several years, its relevant data have been spreaded with various type and media all over the discrete place and inadvertently managed, As a result, managing and assessing data of KRR 1&2 has been difficult and complex and its data cannot be evaluated. By developing the decommissioning database system of KRR 1&2 the data of decommissioning activities can be correctly evaluated, being systematically managed and objectively assessed. Finally, the decommissioning project of KRR 1&2 have come to more effectively accomplished. As the data of decommissioning activities can be at last systematically analyzed and objectively assessed, the decommissioning project of KRR 1&2 would be more effectively accomplished.

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Development and evaluation of MR compatible patch antenna for hyperthermia (온열치료를 위한 MR호환 평판가열안테나 개발 및 성능평가)

  • Kim, D.H.;Chun, S.I.;Jang, M.Y.;Yoon, M.S.;Kim, Y.B.;Jung, B.D.;Nam, S.H.;Mun, C.W.
    • Journal of the Korean Society of Radiology
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    • v.3 no.2
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    • pp.17-21
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    • 2009
  • The thermal treatment have been combined with MRI which is able to acquire both an anatomical image with high-contrast and a thermal image, and have recently used for removing the tumor effectively. This study is to make a patch antenna which is designed to operate at 2.45GHz that has compatibility with MRI. The characteristic and specific absorption rate(SAR) were confirmed using computer simulation and confirmed a possibility of hyperthermia by performing experiment.

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Evaluating The Radioprotective Effect of Avocado Peel Extracts upon Rat Exposed to 6 MV X-Ray (6 MV X-ray에 피폭된 흰쥐에 대한 아보카도 껍질 추출물의 방사선방호효과 평가)

  • Kim, Jang-Oh;Shin, Ji-Hye;Jung, Do-Young;Jeon, Chan-hee;Lee, Ji-Eun;Lee, Yoon-Ji;Min, Byung-In
    • Journal of the Korean Society of Radiology
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    • v.14 no.5
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    • pp.553-561
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    • 2020
  • In this study aims to investigate the radiation protection effect of avocado peel extracts on the Sprague-Dawely rats. 52 male rats were randomly classified into 4 groups. NC Group was a normal control group, PA Group was a group injected avocado peel extracts, IR Group was irradiated group, and lastly PA+IR Group was set as an irradiated group after injected of avocado peel extracts. Avocado peel extract was administered orally at 200 mg/kg once a day for 14 days before irradiation, and the radiation dose was systemically irradiated with 6 MV X-ray of 7 Gy. On the 4 and 21 days after irradiation, the experimental animals were sacrificed to evaluate the change in blood cell composition, spleen index, and histopathological evaluation of the liver and small intestine. As a result, the PA+IR Group showed a significantly greater recovery of lymphocytes(p<0.01), red blood cells(p<0.01), and platelets(p<0.05) than the IR Group. It was also confirmed that the activation of Superoxide Dismutase(SOD) was further increased. Histopathologically, observed that nuclei aggregation and cytoplasmic expansion were slightly reduced in the PA+IR Group in the liver. and the damage was significantly reduce(p<0.01) in the change of villi length due to damage to the small intestine cells. Based on the above results, avocado peel extract can be expected to act as a radiation protection agent that can reduce damage to blood cells and major organs caused by irradiation.

Effects of Recombinant Human Epidermal Growth Factor (rhEGF) on Experimental Radiation-Induced Oral Mucositis in Rats (Rat의 방사선 조사성 구내염에 대한 Recombinant Human Epidermal Growth Factor (rhEGF)의 효과)

  • Jung Kwon-Il;Kim Sun-Hee;Moon Soo-Young;Kim Yeon-Wha;Hong Joon-Pio;Kim Hyun-Sook;Lee Sang-Wook
    • Radiation Oncology Journal
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    • v.24 no.1
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    • pp.67-76
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    • 2006
  • Purpose: Oral mucositis is a common toxicity of radiation or chemotherapy, which is used a treatment for head and neck cancer. We investigated effects of recombinant human epidermal growth factor (rhEGF) on radiation-induced oral mucositis in rat model. Materials and Methods: Spraque-Dawley rats (7 per group) exposed to a single dose of 25 Gy (day 0) on their head, except for one group, were randomly divided into un-treated, vehicle-treated, and two rhEGF-treated groups. Rats were topically applied with rhEGF (15 or $30{\mu}g/oral$ cavity/day) or vehicle to their oral mucosa. Survival rate of rats, weight changes, and food intakes were examined from day 0 to 18 after radiation. Histology study was performed from oral mucosa of rats at day 7 and 18 after radiation. Results: rhEGF-treated groups (15 or $30{\mu}g/oral$) showed all survival rate 33%, whereas un-treated and vehicle-treated groups showed all survival rate 0% at the end of experiment. rhEGF-treated groups statistically had less weight loss compared to vehicle-treated group from day 2 to 7 after radiation. Food intake of rats with rhEGF treatment turned to increase at day 14 after radiation. At 7 day after radiation, un-treated and vehicle-treated groups showed severe pseudomembraneous or ulcerative oral mucositis. On the other hand, rhEGF-treated groups had no more than cellular swelling and degeneration of epidermal cells in oral mucosa of rats. Conclusion: These results suggest that rhEGF has significantly positive effects on radiation-induced oral mucositis in rats. rhEGF display a therapeutic potential on a clinical level.

DIGITAL SUBTRACTION RADIOGRAPHIC EVALUATION OF THE STANDARDIZED PERIAPICAL INTRAORAL RADIOGRAPHS (규격화된 구내 표준 방사선사진의 계수 공제 방사선학적 평가)

  • Cho Bong-Hae;Nah Kyung-Soo
    • Journal of Korean Academy of Oral and Maxillofacial Radiology
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    • v.23 no.1
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    • pp.125-136
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    • 1993
  • The geometrically standardized intraoral radiographs using 5 occlusal registration materials were taken serially from immediate, 1 day, 2, 4, 8, 12, and 16 weeks after making the bite blocks. And the resultant images were digitally subtracted using the immediately taken film as reference images. The qualities of those subtracted images were evaluated to check the degree of reproducibility of each impression material. The results were as follows: 1. The standard deviations of the grey scales of the overall subtracted images were 4.9 for Exal1ex, 7.2 for Pattern resin, 9.0 for Tooth Shade Acrylic, 12.2 for XCP only, 14.8 for Impregum. the lesser the standard deviation, the better the quality of the subtracted images. 2. The standard deviation of the grey scales of the overall subtracted images were grossly related to those of the localized horizontal line of interest. 3. Exaflex which showed the best subtracted image quality had 15 cases of straight, 14 cases of wave, 1 case of canyon shape. Impregum which showed the worst subtracted image quality had 4 cases of straight, 8 cases of wave, 18 cases of canyon shape respectively.

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Cell Image Acquisition and Position Control of the Electron Microbeam System for Individual Cell Irradiation (마이크로 전자빔 개별 세포 조사장치의 세포 영상 획득 및 위치 제어)

  • Park, Seung-Woo;Lee, Dong-Hoon;Hong, Seung-Hong
    • Journal of the Institute of Electronics Engineers of Korea SC
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    • v.42 no.6
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    • pp.49-56
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    • 2005
  • An electron microbeam system has been developed to investigate the biological effect of cells by irradiating cell-nuclei with low-energy and low-flux electrons. It is essential to discern the cell nucleus from its cytoplasm and the culture medium and to locateit exactly onto the beam exit. The irradiation speed at more than 10,000 cells per hour is another requisite for the observations on cellular response to have good statistics. Long-time labor with patience and high concentration is needed since the frames of $320{\times}240{\mu}m^2$ should be moved more than 500 times for irradiating more than 10,000 cells per an hour. This paper describes the electron microbeam system with a focus on the user interfaces concerning the process of automatically recognizing the cell nuclei and injecting electron beam into the target cell nuclei at the irradiation speed of more than 10,000 cell nuclei per hour.