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http://dx.doi.org/10.7742/jksr.2020.14.5.553

Evaluating The Radioprotective Effect of Avocado Peel Extracts upon Rat Exposed to 6 MV X-Ray  

Kim, Jang-Oh (Department of Emergency and Disaster Management, Inje University)
Shin, Ji-Hye (Department of Emergency and Disaster Management, Inje University)
Jung, Do-Young (Department of Emergency and Disaster Management, Inje University)
Jeon, Chan-hee (Department of Emergency and Disaster Management, Inje University)
Lee, Ji-Eun (Department of Emergency and Disaster Management, Inje University)
Lee, Yoon-Ji (Department of Emergency and Disaster Management, Inje University)
Min, Byung-In (Department of Emergency and Disaster Management, Inje University)
Publication Information
Journal of the Korean Society of Radiology / v.14, no.5, 2020 , pp. 553-561 More about this Journal
Abstract
In this study aims to investigate the radiation protection effect of avocado peel extracts on the Sprague-Dawely rats. 52 male rats were randomly classified into 4 groups. NC Group was a normal control group, PA Group was a group injected avocado peel extracts, IR Group was irradiated group, and lastly PA+IR Group was set as an irradiated group after injected of avocado peel extracts. Avocado peel extract was administered orally at 200 mg/kg once a day for 14 days before irradiation, and the radiation dose was systemically irradiated with 6 MV X-ray of 7 Gy. On the 4 and 21 days after irradiation, the experimental animals were sacrificed to evaluate the change in blood cell composition, spleen index, and histopathological evaluation of the liver and small intestine. As a result, the PA+IR Group showed a significantly greater recovery of lymphocytes(p<0.01), red blood cells(p<0.01), and platelets(p<0.05) than the IR Group. It was also confirmed that the activation of Superoxide Dismutase(SOD) was further increased. Histopathologically, observed that nuclei aggregation and cytoplasmic expansion were slightly reduced in the PA+IR Group in the liver. and the damage was significantly reduce(p<0.01) in the change of villi length due to damage to the small intestine cells. Based on the above results, avocado peel extract can be expected to act as a radiation protection agent that can reduce damage to blood cells and major organs caused by irradiation.
Keywords
RadioProtection; Avocado; Antioxidant; Irradiation; Liver; Villi length;
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Times Cited By KSCI : 7  (Citation Analysis)
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1 K. C. Chang, D. U. Lee, "Vasodilatory effect of the alkaloid component from the roots of Cynanchum wilfordi Hemsley", Korean Journal of Life Science, Vol. 10, No. 6, pp. 584-590, 2000. KMID : 0613820000100060584
2 S. Mahgoub, A. O. Sallam, H. K. A. Sarhan, A. A. A. Ammar, S. H. Soror, "Role of Diosmin in protection against the oxidative stress induced damage by gamma-radiation in Wistar albino rats", Regulatory Toxicology and Pharmacology, Vol. 113, 104622, 2020. http://dx.doi.org/10.1016/j.yrtph.2020.104622   DOI
3 J.G Sohn, "Effect of trace elements in alcohol beverages on the type of radiation-induced cell death", Journal of Radiation Protection, Vol. 35, No. 2, pp. 49-56, 2010.
4 International Commission on Radiological Protection, "Annuals of the ICRP Publication 103", pp. 125-129, 2007.
5 M.Z. Kamran, A. Ranjan, N. Kaur, S. Sur, V. Tandon, "Radioprotective Agents: Strategies and Translational Advances", Medicinal Research Reviews, Vol. 36, No. 3, pp. 461-93, 2016. http://dx.doi.org/10.1002/med.21386   DOI
6 M.I. Koukourakis, "Radiation Damage and Radioprotectants: New Concepts in The Era of Molecular Medicine", The British Journal of Radiology, Vol. 85, No. 1012, pp. 313-330, 2012. http://dx.doi.org/10.1259/bjr/16386034   DOI
7 S. Golfier, G. Jost, H. Pietsch, P. Lengsfeld, F. Eckardt-Schupp, E. Schmid, M. Voth, "Dicentric Chromosomes and Gamma-H2AX Foci Formation in Lymphocytes of Human Blood Samples Exposed to a CT Scanner: A Direct Comparison of Dose Response Relationships", Radiation Protection Dosimetry, Vol. 134, No. 1, pp. 55-61, 2009. http://dx.doi.org/10.1093/rpd/ncp061   DOI
8 H. B. Forrester, R. F. Yeh, W. C. Dewey, "A Dose Response for Radiation-induced Intrachromosomal DNA Rearrangements Detected by Inverse Polymerase Chain Reaction", Radiation Research, Vol. 152, pp. 232-238, 1999. http://dx.doi.org/10.2307/3580322   DOI
9 S. J. Hosseinimehr, "Trends in The Development of Radioprotective Agents", Drug Discovery Today, Vol. 12, No. 19, pp. 794-805, 2007. http://dx.doi.org/10.1016/j.drudis.2007.07.017   DOI
10 M. P. Hande, T. V. Azizova, C. R. Geard, L. E. Burak, C. R. Mitchell, V. F. Khokhryakov, E. K. Vasilenko, D. J. Brenner, "Past Exposure to Densely Ionizing Radiation Leaves a Unique Permanent Signature in The Genome", The American Journal of Human Genetics, Vol. 72, No. 5, pp. 1162-1170, 2003. http://dx.doi.org/10.1086/375041   DOI
11 S. V. Gudkov, N. R. Popova, V. I. Bruskov, "Radioprotective Substances: History, Trends and Prospects", Biophysics, Vol. 60, No. 4, pp. 659-667, 2015. http://dx.doi.org/10.1134/S0006350915040120   DOI
12 J. H. Lee, "The Radioprotection Effect of Aronia on Livers of Laboratory RatsRadiated to 6 MV X-ray of Linear Accelerator", Journal of the Korean Society of Radiology, Vol. 14, No. 2, pp. 97-104, 2020. https://doi.org/10.7742/jksr.2020.14.2.97
13 S. I. Jang, J. H. Lee, "Radioprotective Effects of Blueberry on the Liver of Radiation Irradiated Rats," Journal of the Korean Society of Radiology, Vol. 7, No. 3, pp. 239-244, 2013. http://dx.doi.org/10.7742/jksr.2013.7.3.239   DOI
14 Q. Y. Lu, J. R. Arteaga, Q. Zhang, S. Huerta, V. Liang, D. Heber, "Inhibition of Prostate Cancer Cell Growth by an Avocado Extract: Role of Lipid-soluble Bioactive Substances", Journal of Nutritional Biochemistry, Vol. 16, No. 1, pp. 23-30, 2005. http://dx.doi.org/10.1016/j.jnutbio.2004.08.003   DOI
15 W. B. Storey, B. Bergh, G. A. Zentmyer, "The Origin, Indigenous Range, and Dissemination of the Avocado", California Avocado Society, Vol 70, pp. 127-133, 1986.
16 J. A. Vinson, X. Su, L. Zubik, P. Bose, "Phenol Antioxidant Quantity and Quality in Foods: Fruits", Journal of Agricultural and Food Chemistry, Vol. 49, No. 11, pp. 5315-5321, 2001. http://dx.doi.org/10.1021/jf0009293   DOI
17 A. F. Vinha, J. Moreira, S. V. P. Barreira, "Physicochemical Parameters, Phytochemical Composition and Antioxidant Activity of the Algarvian Avocado (Persea americana Mill.)", Journal of Agricultural Science, Vol. 5, No. 12, pp. 100-109, 2013. http://dx.doi.org/10.5539/jas.v5n12p100
18 P. Jimenez, P. Garcia, V. Quitral, K. Vasquez, C. P. Ruiz, M. R. Farias, D. F. G. Diaz, P. Robert, C. Encina, J. S. Covasich, "Pulp, Leaf, Peel and Seed of Avocado Fruit : A Review of Bioactive Compounds and Healthy Benefits", Food Revies International, 2020. http://dx.doi.org/10.1080/87559129.2020.1717520
19 M. A. Tremocoldi, P. L. Rosalen, M. Franchin, A. P. Massarioli, C. Denny, E. R. Daiuto, J. augusto, R. Paschoal, P. S. Melo, S. M. de Alencar, "Exploration of Avocado by-Products as Natural Sources of Bioactive Compounds", PLoS One, Vol. 13, No. 2, pp. 1-12, 2018. http://dx.doi.org/10.1371/journal.pone.0192577
20 D. Haiming, H. Chunhua, G. Dongmei, C. Y. Won, Y. Ding, A. D. Kinghorn, S. M. D. Ambrosio, "Selective Induction of Apoptosis of Human Oral Cancer Cell Lines by Avocado Extracts Via a ROS-Mediated Mechanism", Nutrition and Cancer, Vol. 61, No. 3, pp. 348-356, 2009. http://dx.doi.org/10.1080/01635580802567158   DOI
21 J. A. O. Ojewole, G. J. Amabeoku, "Anticonvulsant effect of Persea americana Mill (Lauraceae) (Avocado) leaf aqueous extract in mice", Phytotherapy Research, Vol. 20, No. 8, pp. 696-700, 2006. http://dx.doi.org/10.1002/ptr.1940   DOI
22 J. J. Guzman-Rodriguez, R. Lopez-Gomez, L. M. Suarez-Rodriguez, R. Salgado-Garciglia, L. C. Rodriguez-Zapata, A. Ochoa-Zarzosa, J. E. Lopez-Meza, "Antibacterial Activity of Defensin PaDef from Avocado Fruit (Persea Americana Var. Drymifolia) Expressed in Endothelial Cells against Escherichia Coli and Staphylococcus Aureus", Biomed Research International, Vol. 2013, pp. 1-9, 2013. http://dx.doi.org/10.1155/2013/986273
23 O. P. Mendez, L. G. Hernandez, "High-density Lipoproteins(HDL) Size and Composition are Modified in the Rat by a Diet Supplemented with "Hass" Avocado (Persea Americana Miller)", Archivos de Cardiologia de Mexico, Vol. 77, No. 1, pp. 17-24, 2006. PMID : 17500188
24 U. S. Mahadeva Rao, "Salutary Potential of Ethanolic Extract of Avocado Fruit on Anomalous Carbohydrate Metabolic Key Enzymes in Hepatic and Renal Tissues of Hyperglycaemic Albino Rats", Chinese Journal of Integrative Medicine, 2017. http://dx.doi.org/10.1007/s11655-017-2784-2
25 S. G. Lee, M. H. Yu, S. P. Lee, I.S. Lee, "Antioxidant Activities and Induction of Apoptosis by Methanol Extracts from Avocado", Journal of Korean Society of Food Science and Nutrition, Vol. 37, No. 3, pp. 269-275, 2008. http://dx.doi.org/10.3746/JKFN.2008.37.3.269   DOI
26 O. Ilochi, A. N. Chuemere, "Neuroprotective Potential of Avocado Peel Correlates with Antioxidant Status in Starvation and Refeeding In Wistar Rats", World Wide Journal of Multidisciplinary Research and Development, Vol. 5, No. 1, pp. 70-74, 2019.
27 D. Y. Jung, H. S. Choi, J. O. Kim, J. H. Shin, J. H. Kim, G. J. Park, B. I. Min, "Radiation Protective Effect of Selenium and Folic Acid Mixtures in the Development of Congenital Anomalies Following Radiation Exposure to the Fetus of Perinatal Female White Rats", Journal of Radiological Science and Technology, Vol. 41, No. 2, pp. 157-162, 2018. http://dx.doi.org/10.17946/JRST.2018.41.2.157   DOI
28 M. J. Cires, P. Navarrete, E. Pastene, C.Carrasco-Pozo, R. Valenzuela, D.A. Medina, M. Andriamihaja, B. Martin, B. Francois, M. Gotteland, "Effect of a proanthocyanidin-rich polyphenol extract from avocado on the production of amino acid-derived bacterial metabolites and the microbiota composition in rats fed a high-protein diet", Food & Function, Vol 10, No. 7, pp. 4022-4035, 2019. http://dx.doi.org/10.1039/C9FO00700H   DOI
29 O. Gencel, M. Naziroglu, O. Celik, K. Yalman, D. Bayram, "Selenium and Vitamin E Modulates Radiation-Induced Liver Toxicity in Pregnant and Nonpregnant Rat: Effects of Colemanite and Hematite Shielding", Biological Trace Element Research, Vol. 135, pp. 253-263, 2010. http://dx.doi.org/10.1007/s12011-009-8513-8   DOI
30 M. J. Cheong, K. A. Chung, "Effects of Lespedeza Caneata ethanol extract on the liver of alcohol administered Mice", Journal of the Korea Academia-Industrial cooperation Society, Vol. 17, No. 10, pp. 432-438, 2016. http://dx.doi.org/10.5762/KAIS.2016.17.10.432   DOI
31 G. P. Joshi, W. J. Nelson, S. H. Revell, C. A. Shaw, "X-ray-induced Chromosome Damage in Live Mammalian Cells, and Improved Measurements of Its Effects on Their Colony-forming Ability", International Journal of Radiation Biology, Vol. 41, No. 2, pp. 161-242, 1982. http://dx.doi.org/10.1080/09553008214550171
32 J. H. Kim, S. B. Sohn, S. K. Kim, Y. B. Hur, "Effects on hematological parameters, antioxidant and immune responses, AChE, and stress indicators of olive flounders, Paralichthys olivaceus, raised in bio-floc and seawater challenged by Edwardsiella tarda", Fish and Shellfish Immunology, Vol. 97, pp. 194-203, 2020. http://dx.doi.org/10.1016/j.fsi.2019.12.011   DOI