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Effects of Recombinant Human Epidermal Growth Factor (rhEGF) on Experimental Radiation-Induced Oral Mucositis in Rats  

Jung Kwon-Il (Departments of Toxicology and Pharmacology, Institute of Bioscience and Biotechnology, Daewoong Pharmaceutical Co., LTD.)
Kim Sun-Hee (Departments of Protein Drug Development, Institute of Bioscience and Biotechnology, Daewoong Pharmaceutical Co., LTD.)
Moon Soo-Young (Departments of Radiation Oncology, University of Ulsan College of Medicine, Asan Medical Center)
Kim Yeon-Wha (Departmants of Plastic Surgery Oncology, University of Ulsan College of Medicine, Asan Medical Center)
Hong Joon-Pio (Departmants of Plastic Surgery Oncology, University of Ulsan College of Medicine, Asan Medical Center)
Kim Hyun-Sook (Department of Radiology, Gyeongsang National University College of Medicine)
Lee Sang-Wook (Departments of Radiation Oncology, University of Ulsan College of Medicine, Asan Medical Center)
Publication Information
Radiation Oncology Journal / v.24, no.1, 2006 , pp. 67-76 More about this Journal
Abstract
Purpose: Oral mucositis is a common toxicity of radiation or chemotherapy, which is used a treatment for head and neck cancer. We investigated effects of recombinant human epidermal growth factor (rhEGF) on radiation-induced oral mucositis in rat model. Materials and Methods: Spraque-Dawley rats (7 per group) exposed to a single dose of 25 Gy (day 0) on their head, except for one group, were randomly divided into un-treated, vehicle-treated, and two rhEGF-treated groups. Rats were topically applied with rhEGF (15 or $30{\mu}g/oral$ cavity/day) or vehicle to their oral mucosa. Survival rate of rats, weight changes, and food intakes were examined from day 0 to 18 after radiation. Histology study was performed from oral mucosa of rats at day 7 and 18 after radiation. Results: rhEGF-treated groups (15 or $30{\mu}g/oral$) showed all survival rate 33%, whereas un-treated and vehicle-treated groups showed all survival rate 0% at the end of experiment. rhEGF-treated groups statistically had less weight loss compared to vehicle-treated group from day 2 to 7 after radiation. Food intake of rats with rhEGF treatment turned to increase at day 14 after radiation. At 7 day after radiation, un-treated and vehicle-treated groups showed severe pseudomembraneous or ulcerative oral mucositis. On the other hand, rhEGF-treated groups had no more than cellular swelling and degeneration of epidermal cells in oral mucosa of rats. Conclusion: These results suggest that rhEGF has significantly positive effects on radiation-induced oral mucositis in rats. rhEGF display a therapeutic potential on a clinical level.
Keywords
Oral mucositis; Radiation; Epidermal growth factor; Rat model;
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